Leica DMi8 Instructions For Use Manual
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Leica DMi8 Instructions For Use Manual

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Leica DMi8
Instructions for Use Gebrauchsanweisung Mode d'emploi
Leica Microsystems CMS GmbH Instructions 11934056, Revision 1.1 from 2015-07-15

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  • Page 1 Leica DMi8 Instructions for Use Gebrauchsanweisung Mode d’emploi Leica Microsystems CMS GmbH Instructions 11934056, Revision 1.1 from 2015-07-15...
  • Page 2 COPYRIGHT/ CONTENTS...
  • Page 3 Leica DMi8 Instructions for Use Leica Microsystems CMS GmbH Instructions 11934056, Revision 1.1 from 2015-07-15...

  • Page 4: Table Of Contents

    COPYRIGHT/ CONTENTS Copyright Contents All rights to this documentation are held by Leica Micro- Important Notes about this Manual ......4 systems CMS GmbH. Reproduction of text or illustrations Text symbols and their meanings ......4 (in whole or in part) by print, photocopy, microfilm or other Intended Purpose of the Microscope ......
  • Page 5 Filter ................62 5.11.1 Transmitted Light filter ..........62 5.11.2 Fluorescence Cubes ..........63 5.11.3 Leica RFID Module............65 5.11.3.1 Product Description ..........65 5.11.3.2 Specifications LID Module 1.0 .......65 5.11.3.3 Specifications RFID Tag D6.7 special ..... 65 5.11.3.4 Ports ................65 5.11.3.5 FCC / IC Compliance ..........

  • Page 6: Important Notes About This Manual

    1. IMPORTANT NOTES ABOUT THIS MANUAL 1 Important Notes about this Manual Caution! These Instructions for Use are an essential component of the product; they must be read carefully before the product is assembled, put into operation or used, and must be kept for later reference.
  • Page 7 1. IMPORTANT NOTES ABOUT THIS MANUAL Warning of hazardous electrical voltage! Risk of electrical shock! Danger due to hot surface Warning of optical radiation! Never look directly into the light beam! Wear safety goggles! Warning of electromagnetic fi eld Warning of UV radiation! Wear safety goggles! Warning of hazardous laser radiation –...

  • Page 8: Intended Purpose Of The Microscope

    Council Directives 2006/95/EC and 2014/35/EC concerning electrical apparatus and 2004/108/ EC and 2014/30/EC con- The Leica DMi8 series can be used universally. All con- cerning electromagnetic compatibility. trast methods, such as Brightfield, Darkfield, Phase Contrast, DIC, fluorescence or modulation contrast, are integral components of the microscope and can be adapted or exchanged quickly and easily.
  • Page 9 Leica Microsystems CMS GmbH offers special micro- scope variants with additional safety features. Laser couplings require corresponding safety devices that have to be inspected and installed by trained personnel. For further information, please contact your authorized...

  • Page 10: Safety Notes

    61326-1 Electrical equipment junction with non-Leica components that are outside of for measurement, control and laboratory use – EMC re- the scope of this manual. quirements . Unauthorized alterations to the instrument or noncom-...
  • Page 11 3. SAFETY NOTES Advanced/Advanced+ Leica CTR electronics box Leica DMi8 automated For indoor use only. For indoor use only. Supply voltage: 100 – 240 VAC Supply voltage: 100 – 240 VAC ( Leica CTR) Frequency: 50 / 60 Hz Frequency:...
  • Page 12 For con- nections without a ground protection conductor, Leica Microsystems CMS GmbH Service must be consulted. WARNING By definition, the main circuit breaker of this instrument...

  • Page 13: Laser Safety

    3. SAFETY NOTES Laser safety Two equivalent interlock connectors, which lead to the microscope's interlock switch, are attached to the back of 3.3.1 Safety switch (interlock) on the microscope the microscope. The interlock connector of the microscope Two safety switches are integrated into the microscope us- must be connected to your laser beam interrupter.

  • Page 14: Screwed-On Filter Turret Cover

    Because of a danger for the user due to laser radiation, the fluorescent turret disk cover is secured using 2 Leica DMi8 microscopes in the standard version are not suitable without additional laser safety measures for cou- DIN 912 M4x6 lens head Allen screws.

  • Page 15: Safety Hazard Due To Folded Back

    3. SAFETY NOTES 3.3.3.1 Safety hazard due to folded back Transmitted Light arm If you fold back the Transmitted Light arm, the laser radia- tion escaping from the lenses is beamed directly into the open space. Important safety note As a safety measure, we recommend the microscope models with integrated interlock connectors as men- tioned in Section 3.3.3.7.

  • Page 16: Safety Hazard Above The Condenser

    3. SAFETY NOTES 3.3.3.4 Safety hazard above the condenser The laser beam path between the condenser base and the illuminated field diaphragm is without obstruction. Laser beam path between condenser base and illuminated field dia- phragm without obstruction 3.3.3.5 Safety hazard in the specimen area The laser radiation is accessible without obstruction in the specimen area of the microscope! Specimen area...

  • Page 17: Safety Hazard Due To Openings At Microscope

    3. SAFETY NOTES 3.3.3.6 Safety hazard due to openings at microscope Furthermore, the terminals that are not occupied by objec- Make sure, as part of your risk assessment, that all open- tives have to be closed with caps at the objective nose- ings at the microscope are tightly sealed and can only be piece.

  • Page 18: Note Regarding The Safety Hazards In Sections 3.3.3.1 And Section 3.3.3.2

    Leica Microsystems CMS GmbH listed below in laser beam coupled into the microscope when opening the conjunction. Within the context of your system composi- interlock switch or folding back the illumination arm.

  • Page 19: Exception For Class 1 Lasers

    3. SAFETY NOTES When doing so, note the electrical characteristic values of 3.3.4. Exception for Class 1 lasers the integrated interlock switches in the microscope. You Coupling a laser of Class 1 in accordance with IEC/EN must not exceed these values by connecting a laser beam 60825-1 into a microscope does not result in any danger to interrupter, as otherwise the interlock switches will be the user.

  • Page 20: Storage And Transport

    3. SAFETY NOTES Storage and transport Weight CAUTION Transport At a full load, the microscope weighs more than 18 Caution! kg. The user must take corresponding precautions for transporting the instrument. Transport and storage at -20 °C – +85 °C and max. 90% humidity.

  • Page 21: Notes On Handling Light Sources

    During the toggling procedure, the maximum radiant power of the light source may be present on the eye- pieces for a short time and can cause blindness in the user! +) Up to 40°C in microscope climate chambers sold by Leica Microsystems GmbH...

  • Page 22: Notes On Handling The Touch Screen

    Disposal CAUTION Once the product has reached the end of its service life, please contact Leica Microsystems GmbH Service or Sales Lamps and lamp housing may be hot! about disposal. They must be placed at least 10 cm away from the wall and away from flammable substances.

  • Page 23: Leica Dmi8 Configurations Instrument Overview

    4. Leica DMi8 configurations instrument overview Microscope configurations (examples) Leica DMi8 with manual components Leica DMi8 with manual components With a fixed Transmitted Light arm With a tiltable Transmitted Light arm Leica DMi8 with encoded components Leica DMi8 with at least one motorized component...

  • Page 24: Dimensions (Specified In Mm)

    4. OVERVIEW OF THE INSTRUMENT Dimensions (specified in mm) Leica CTR compact Weight: approx. 2 kg Leica CTR Advanced/Advanced+ Weight: approx. 4 kg...
  • Page 25 4. OVERVIEW OF THE INSTRUMENT Leica DMi8 (with tiltable Transmitted Light arm) Weight: approx. 19 kg Height compensation plate* In order to enlarge the viewing height by 23 mm, raise the side camera ports for very large cameras or spinning disks,...

  • Page 26: Specifications +)

    4. OVERVIEW OF THE INSTRUMENT Specifications Leica DMi8 series - depending on Transmitted Light arm Contrast methods IPH (Integrated Phase Contrast) Leica DMi8 with motorized condenser and motorized fluorescence Transmitted Light arm Tiltable Transmitted Light arm micromanipulators ≤ S40 condensor with an integrated manual or motorized illuminated field dia-...
  • Page 27 4. OVERVIEW OF THE INSTRUMENT Fluorescence axis Manual, external (Reduces the light intensity of the incident light illumination) Manually integrated (Reduction of light intensity from the incident light illumination) Motorized (Reduction of light intensity from the incident light illumination) Infinity port and T-Houses With 2 possible positions: 50% rear port/50% left/right port 80% rear port/20% left/right port...
  • Page 28 4. OVERVIEW OF THE INSTRUMENT Magnification Changer Motorized Magnification factors 1x; 1.6x; 2x or 1x; 1.6x or 1x; 2x Manual Magnification factors 1x; 1.6x Objective turret motorized objectives with M25 threads and a 45mm parfocalizing distance encoded Manual For DIC: manual Wollaston prism turntable Stages Heated stage plate (3°C above room temperature up to 60°C) (248 x 212 mm) Size of inserts:160 mm x 110 mm (compatible with scanning stages)
  • Page 29 4. OVERVIEW OF THE INSTRUMENT Condensers For tiltable Transmitted Light arms: For the fixed Transmitted Light arm Z-focus Leica DMi8 motorized ≤ 20 nm Leica DMi8 manual Observation ports Leica DMi8 motorized Leica DMi8 manual...

  • Page 30: Controls

    The Touch Screen is available as a control element on the Leica DMi8 with at least one motorized component. The external controls are the Leica SmartMove control and the Leica STP4000 and Leica STP8000 control panels.

  • Page 31: Control Panel Fields

    The number of buttons may vary depending on the configu- ration. Control panel fields on the front side Swiveling Touch Screen for adjusting all automated compo- nents on the Leica DMi8. Adjustable angle for better readability. Refer also to the chapter titled "The Touch Screen" S. 34...
  • Page 32 4. OVERVIEW OF THE INSTRUMENT Encoded objective nosepiece 6 LEDs for indicating the current objective Label fields Encoded objective nosepiece and encoded fluo turret 6 LEDs for indicating the filter cube Label fields 6 LEDs for indicating the current objective Motorized objective nosepiece 6 objective changer buttons with LEDs for indicating the current objective...

  • Page 33: Additional Control Elements

    4. OVERVIEW OF THE INSTRUMENT 4.4.2 Additional control elements Left side of the Leica DMi8 with a fixed Transmitted Light Filter receptacle Aperture Diaphragm Adjustment Stop lever for condenser height adjustment IMC or light ring slide Bertrand lens centering Toggling the top port and Bertrand lens...
  • Page 34 Selecting the objective prisms Manual DIC objective prism disk Motorized DIC objective prism disk Right side of the Leica DMi8 with a tiltable Transmitted Light arm Switching the Transmitted Light filter on and off The front position is assigned at the factory using a shutter (dark stop), which is interchangeable with a fil-...
  • Page 35 4. OVERVIEW OF THE INSTRUMENT Right side of Leica DMi8 with manual integrated fluores- cence axis Manual Magnification Changer Focusing Manual filter change Receptacle for analyzer Toggling the left side port Stage movement On/off switch (only on manual stands) Illuminated field diaphragm adjustment Manual setting for FIM 10 Centering illuminated field diaphragm...

  • Page 36: The Touch Screen

    4.4.3 The Touch Screen The Touch Screen adjusts all motorized components of the Leica DMi8 automatically via buttons. After the device is switched on, the display shows the current microscope status. The display and functions that can be operated us- ing the Touch Screen depend on the features of the indi- vidual microscope.
  • Page 37 4. OVERVIEW OF THE INSTRUMENT Note: Before working with the touch screen, we recommend cali- brating it first. Calibration button. button. working area using your finger. Press and hold the cali- bration cross until the next cross is displayed. This ad- justs the sensitivity of the Touch Screen and the pressure surface to the user.

  • Page 38: The Leica Smartmove Control Element

    To lock the controls, press and hold the two front keys (5) simultaneously for 5 seconds. Repeat this process to unlock the controls. The status LED (6) indicates whether the Leica SmartMove is in normal mode or locked mode. Red LED...

  • Page 39: Leica Stp4000/8000 External Control Panel

    The Leica STP4000 enables remote control of all automated microscope functions using an external Touch Screen. The Leica STP8000 control panel also enables monitoring in X, Y and Z and has user-definable function keys. Separate instructions are provided for operation of the Leica STP4000/8000.

  • Page 40: Operation

    For information about using the LAS X software, refer to the The microscope may only be operated by personnel who corresponding online help. have been instructed by an authorized Leica employee! Note: If at all possible, avoid touching optical surfaces such as the lens surfaces on the objective.

  • Page 41: Switching On The Microscope

    The indicator lamp is illuminated in green during operation. Leica DMi8 with at least one motorized component and Leica CTR electronics box indicator lamp is illuminated in green during operation. Note: If you have connected a PC, switch on the electronics box first, and then the computer.
  • Page 42 After the initialization is completed, the Touch Screen shows the status screen with the current microscope set- ting. For fluorescence: Leica EL6000 compact light source on the on/off switch. The indicator lamp is illuminated during op- eration. CAUTION Do not switch on the light source until the light guide is connected to the microscope.

  • Page 43: Illumination

    To switch it on again, press the rotary knob for the light in- tensity. Leica DMi8 with automated fluorescence the TL/Fluor key (TL). stand.

  • Page 44: Fluorescence

    The intensity is individually adjusted and stored for each objective and contrast method. 5.2.2 Fluorescence Leica DMi8 with manual encoded fluorescence (Fluor) by opening the shutter (pull out switch rod at the manual integrated fluorescence axis or on the manual ex- ternal fluorescence axis).
  • Page 45 The brightness is adjusted in 5 defined increments (FIM) using a slider: 100% / 55% / 30% / 17% / 10% Note: If the Leica DMi8 is equipped with a 2 port infinity port ( S. 44) switch over to the current port . S. 58 Manual integrated fluorescence axis...

  • Page 46: Infinity Port

    5. OPERATION - ILLUMINATION Fluorescence Intensity Manager (FIM): The brightness is adjusted in 5 defined increments on the brightness adjusting knob: 100% / 55% / 30% / 17% / 10% Setting the fluorescence using the Touch Screen: with the IL button. The brightness is adjusted in 5 defined increments on the brightness adjusting knob: 100% / 55% / 30% / 17% / 10%...

  • Page 47: Shutter

    5. OPERATION - ILLUMINATION 5.2.4 Shutter Leica DMi8 with manual shutter on the manually integrated fluorescence axis or using the switch rod on the manual external fluorescence axis. Manual integrated fluorescence axis Manual external fluorescence axis Leica DMi8 with motorized shutter side of the stand.

  • Page 48: Objectives

    Keypad for encoded objective nosepiece b Keypad for encoded objective nosepiece and coded fluo turret Leica DMi8 with motorized objective nosepiece and front operating panel with function keys jective changer button on the keypad of the front side of the microscope.
  • Page 49 Leica DMi8 - automated variants The objectives can be swung into the beam path by mo- torized action using variable function keys on the stand or Leica SmartMove or using the Touch Screen. Note: The positions of the objectives in the objective turret have been specified at the factory and must be observed when...
  • Page 50 5. OPERATION - OBJECTIVES Immersion objectives immersion objectives use the appropriate immersion medium. OIL: Use optical immersion oil in accordance with DIN/ ISO standards only Cleaning S. 89 Water immersion IMM: Universal objective for water, glycerin, Oil immersion Caution! Follow safety data sheet for immersion oil! The objective nosepiece is lowered to the lower thres- hold.

  • Page 51: Tube

    5. OPERATION - TUBE Tube There are also Leica DMi8 without tube. Note: Close any unused tube openings, as otherwise stray light can interfere with observation. Adjusting the interpupillary distance a congruent total image is seen. Adjusting the viewing angle from 30°...

  • Page 52: Eyepieces

    5. OPERATION - EYEPIECES Eyepieces Note: The eyepiece's aperture protector must be removed or folded back during microscopy while wearing eyeglasses. Protection screen We recommend removing bifocals and spectacles with pro- gressive-addition lenses when using the microscope. Adjusting the eyelens choose the 100% VIS position.

  • Page 53: Stages And Object Displacement

    Motorized 3-plate cross-stage Object displacement in the x- and y-direction can be per- formed by the Leica SmartMove control element, the Leica STP8000 main switch board or LAS X software. Move in x-direction Scanning stage/ linear motorized stage Also read the separately provided instruction manual.

  • Page 54: Adjusting The Step Sizes On Motorized Stages

    5. OPERATION - STAGES AND OBJECT DISPLACEMENT 5.6.2 Adjusting the step sizes on motorized stages key to switch to stage and focus controls. The X/Y coordinates of the cur- rent stage position are displayed. Stage menu using the key. travel speed of the stage by switching between the Coarse and Fine step sizes.
  • Page 55 5. OPERATION - STAGES AND OBJECT DISPLACEMENT Up to 5 stage positions and one load position (L) can be stored. + and – keys the key If a stage position was previously saved in this location, it will be overwritten. When driving to the load position, the objective nose- piece is raised to the lower threshold.

  • Page 56: Focusing

    Leica DMi8 manual focus left and right sides of the stand. Coarse focusing Fine focusing Leica DMi8 with motorized focus/closed loop Focusing Leica SmartMove control element. focus dials on the left and right sides of the stand can also be used.

  • Page 57: Focus Thresholds

    LAS X software. Note: Right side of Leica DMi8 with motorized focus The focus position and stops are stored by the microscope 1 Focusing and retained from one session to the next when power is 2 Focus stop switched off.

  • Page 58: Adjusting The Step Sizes

    Set or Delete key. Focus position Lower stop 5.7.3 Adjusting the step sizes Leica DMi8 with motorized focus/closed loop Coarse and Fine to switch between coarse and fine focusing at the stand handwheel or the focus knob of the Leica SmartMove as applicable.

  • Page 59: Afc (Adaptive Focus Control)

    5. OPERATION - FOCUSING 5.7.4 AFC (Adaptive Focus Control) Leica DMi8 with motorized focus/closed loop The AFC can be used to actively hold a defined focus posi- tion and automatically readjust this position regularly. This is useful if, for example, a temperature change is caused by opening the climate chamber during living cell experiments at 37°C.

  • Page 60: Selecting The Ports

    Left side port Bottom port Note: The "Select the bottom port" function can be assigned to one of the variable function keys on the stand or Leica SmartMove. Note: Side ports not in use must by covered with cover caps.

  • Page 61: Magnification Changer

    5. OPERATION - MAGNIFICATION CHANGER Magnification Changer Leica DMi8 with manually encoded Magnification Changer As an option, a mechanical Magnification Changer can be used. Magnification factors: 1x; 1.6x tion factor. Note: The mechanical Magnification Changer works on the eye- piece and the top port.

  • Page 62: Aperture Diaphragm And Field Diaphragm

    5. OPERATION - APERTURE AND ILLUMINATED FIELD DIAPHRAGM 5.10 Aperture diaphragm and field diaphragm Leica DMi8 with manual aperture diaphragm Leica DMi8 with a fixed Transmitted Light arm For a tiltable Transmitted Light arm: denser. Manual aperture diaphragm For a fixed Transmitted Light arm: Leica DMi8 with manual illuminated field diaphragm...
  • Page 63 5. OPERATION - APERTURE AND ILLUMINATED FIELD DIAPHRAGM Leica DMi8 with motorized aperture and illuminated field diaphragm Both diaphragms have been set to suitable values for the current objective and contrast method at the factory. Aperture (aperture diaphragm) or Field (field diaphragm) keys, the motorized diaphragms can be changed at any time.

  • Page 64: Filter

    Note: The front position is assigned at the factory using a shutter (dark stop), which is interchangeable with a filter. For a fixed Transmitted Light arm: Leica DMi8 with a fixed Transmitted Light arm: Transmitted Light filter ted Light arm.

  • Page 65: Fluorescence Cubes

    The LED allocated to the filter cube and located on the front side of the microscope is illuminated. Leica DMi8 with motorized fluorescent turret disk and front operating panel with function keys ter cube changer button on the keypad of the front side of the microscope.
  • Page 66 (Order No. 11 703 121 630 000) Detach both screws. WARNING Store the screws in a safe place. Never operate the microscope when the cover is not screwed into place! Change the filter cubes as described for the manual Leica DMi8.

  • Page 67: Leica Rfid Module

    D=6,7mm TH=2,6mm Changes or modifications made to this equipment not ex- RFID Protocol ISO 15693 pressly approved by Leica Microsystems CMS GmbH may void the FCC authorization to operate this equipment. Transmit frequncy 13,56 MHz Read write type 2kbit EEProm...

  • Page 68: The Memory Function On The Touch Screen

    Leica DMi8 with manual Transmitted Light method sic microscope settings. The contrast method on the manual or encoded Leica DMi8 can be operated using the manual condenser, the manual objective nosepiece, or the rotary knobs and slide bars on Memory Function menu using the key.

  • Page 69: Brightfield (Tl)

    PI-TL" filter cube using LAS X software. Transmitted Light contrast methods are possible with this filter cube. 5.13.1 Brightfield (TL) Leica DMi8 with manual Transmitted Light method Toggling Transmitted Light/fluorescence position on the fluo turret, or select the "DAPI-TL" filter cube.
  • Page 70 5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD illumination. Leica DMi8 with motorized Transmitted Light method BF (Brightfield). The Brightfield position is approached for the motorized Condenser setting for tiltable Transmitted Light arm - con- condenser. figuring BF position An empty position or the filter cube "DAPI-TL" on the fluo- rescent turret disk is approached automatically.

  • Page 71: Phase Contrast (Tl)

    5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD 5.13.2 Phase Contrast (TL) (Integrated Phase Contrast, see 5.13.6) Leica DMi8 with manual Transmitted Light method position on the fluo turret, or select the "DAPI-TL" filter cube. suitable for Phase Contrast are engraved with PH.
  • Page 72 5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD lyzer, polarizer or IC prisms, from the beam path. Leica DMi8 with motorized Transmitted Light method PH contrast method (Phase Contrast). The correct light ring on the motorized condenser is swung inwards. Objectives that are suitable for Phase Contrast are en- graved with PH.

  • Page 73: Darkfield (Tl)

    5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD 5.13.3 Darkfield (TL) Leica DMi8 with manual Transmitted Light method position on the fluo turret, or select the "DAPI-TL" filter cube. condenser. lyzer, polarizer or IC prisms, from the beam path. Condenser setting for tiltable Transmitted Light arm - con- figuring Darkfield stop (light ring)

  • Page 74: Polarization (Tl)

    5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD 5.13.4 Polarization (TL) Leica DMi8 with manual Transmitted Light method position on the fluo turret, or select the "DAPI-TL" filter cube. Swinging in polarizer polarizer on the condenser into the beam path. analyzer on the right side of the stand up to the click stop.
  • Page 75 5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD Leica DMi8 with motorized Transmitted Light method POL (Polarization) contrast method Motor procedure: POL contrast method has been selected, the polarizer is swung in (if the microscope is equipped with these components). The analyzer cube is also automati- cally brought into the beam path.

  • Page 76: Differential Interference Contrast - Dic (Tl)

    5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD 5.13.5 Differential Interference Contrast – DIC (TL) Leica DMi8 with manual Transmitted Light method position on the fluo turret, or select the "DAPI-TL" filter cube. Swinging in polarizer the condenser. the objective nosepiece.
  • Page 77 5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD Leica DMi8 with motorized Transmitted Light method DIC (Differential Interference Contrast) con- trast method. denser prisms are automatically brought into the beam path. The corresponding objective prism and the analyzer cube are also approached automatically.

  • Page 78: Integrated Phase Contrast - Iph (Tl)

    5.13.6 Integrated Phase Contrast – IPH (TL) Note: The Integrated Phase Contrast requires a condenser on the tiltable Transmitted Light arm. Leica DMi8 with manual Transmitted Light method position on the fluo turret, or select the "DAPI-TL" filter cube. Receptacle for Phase Contrast module...
  • Page 79 5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD Leica DMi8 with motorized Transmitted Light method PH (Phase Contrast) contrast method. The correct light ring on the motorized condenser is swung inwards. (eyepoint B or C). the Phase Contrast module evenly from the front on the left side of the microscope.

  • Page 80: Integrated Modulation Contrast - Imc (Tl)

    5.13.7 Integrated Modulation Contrast – IMC (TL) IMC module for tiltable Transmitted Light arm Position for objectives with eyepoint C Leica DMi8 with manual Transmitted Light method Position for objectives with eyepoint B Brightfield position position on the fluo turret, or select the "DAPI-TL" filter cube.
  • Page 81 5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD Setting the IMC slit diaphragms: For a tiltable Transmitted Light arm: Swinging in polarizer nation for the current magnification. polarizer on the condenser into the beam path. Note: The position of the polarizer influences the appearance of the relief.
  • Page 82 5. OPERATION - TRANSMITTED LIGHT CONTRAST METHOD Fine adjustment: For a tiltable Transmitted Light arm: IMC module for tiltable Transmitted Light arm on the IMC modulator and the polarizer. just the slit diaphragms to the focal plane. This setting depends on the specimen being observed. Note: The position of the polarizer influences the appearance of the relief.
  • Page 83 5. OPERATION - FLUORESCENCE Leica DMi8 with motorized Transmitted Light method able the DIC button! The correct slit diaphragm is configured and the polarizer is automatically swung inwards on the motorized con- denser. (eyepoint B or C). IMC module for tiltable Transmitted Light arm...

  • Page 84: Fluorescence

    Manual external fluorescence axis Manual shutter Note: Manual setting for FIM If the Leica DMi8 is equipped with a 2 port infinity port ( S. 44) switch over to the current port . S. 58 Connection for light guide...
  • Page 85 5. OPERATION - FLUORESCENCE Leica DMi8 with manually encoded fluorescent turret disk filter cubes are swiveled manually into the beam path by turning the fluorescent turret disk. For encoded fluorescence For motorized fluorescent The LED allocated to the filter cube and located on the...
  • Page 86 5. OPERATION - FLUORESCENCE incident light shutter. To do so, push the IL shutter button. SmartMove or using the focus dial. Leica EL6000 compact light source. (See separate Instructions for Use). Fluorescence Intensity Manager (FIM): The brightness is adjusted in 5 defined increments on the...

  • Page 87: Combination Method

    5. OPERATION - COMBINATION METHOD 5.15 Combination method Leica DMi8 with motorized Transmitted Light method and motorized fluorescence: Depending on the configuration of your microscope, up to two combination methods are possible: FLUO/PH and FLUO/DIC key to configure the contrast method.

  • Page 88: Troubleshooting

    6. TROUBLESHOOTING 6. Troubleshooting Problem Cause/remedy Stand The microscope does not respond. Ensure that the AC outlet has power. Make sure that the electronics box or the microscope is connected to the power supply. Check the cable connections. Inform Service and have the supply unit fuse checked. Illumination The image is completely dark.
  • Page 89 6. TROUBLESHOOTING Problem Cause/remedy Darkfield No definite DF contrast is possible. Be sure that a DF objective is being used. The objective aperture setting is too high: Maximum 0.7 for condenser S1 Maximal 0.4 for condenser S23/28/40 If necessary, reduce the objective aperture using the iris diaphragm on the objective.
  • Page 90 6. TROUBLESHOOTING Problem Cause/remedy Transmitted-light interference contrast No Transmitted Light interference contrast is possible. The specimen is too thick or too thin. The mounting medium or specimen are of birefringent material. Rotate the specimen. The difference in the refractive indices of the specimen and the mounting medium is too small.

  • Page 91: Care Of The Microscope

    7. CARE OF THE MICROSCOPE 7. Care of the Microscope Cleaning Caution! Caution: Unplug the power supply before performing cleaning Residual fiber and dust can create unwanted background and maintenance work! fluorescence during fluorescence microscopy. Protect electrical components from moisture! Cleaning coated parts Microscopes in warm and warm-damp climatic zones re- Dust and loose dirt particles can be removed with a soft...

  • Page 92: Handling Acids And Bases

    7. CARE OF THE MICROSCOPE Cleaning glass surfaces and objectives Removing immersion oil The cleaning of glass surfaces and objectives in particular should be carried out exclusively as outlined in the bro- Caution! chure "Cleaning of Microscope Optics". The information can Follow safety notes for immersion oil! be downloaded at: First, wipe off the immersion oil with a clean cotton cloth,...

  • Page 93: Essential Consumable And Spare Parts

    8. ESSENTIAL CONSUMABLE AND SPARE PARTS 8. Essential Consumable and Spare Parts Order No. Material No. Name Used for Screw cap for unused objective receptacles 020-422-570-000 Screw cap M 25 Objective nosepiece Cover for unoccupied objective-DIC-disk opening 11 090-144-020-088 DIC cover microscope stand Dust and light stop for analyzer opening 11 020-437-101-013...

  • Page 94: Appendix

    – ASSIGNING THE VARIABLE FUNCTION KEYS 9. APPENDIX 9. Appendix Assigning the variable function keys The variable function keys can always be defined by the user via the LAS X software. Button 2 Button 1 Depending on the configuration, useful functions are al- ready assigned to the function key upon delivery.
  • Page 95 – ASSIGNING THE VARIABLE FUNCTION KEYS 9. APPENDIX Possible assignments of the variable function keys to the stand and SmartMove: Function button Function Brightfield Transmitted Light Phase Contrast Transmitted Light Interference contrast, Transmitted Light Darkfield Transmitted Light Polarization Transmitted Light CHANGE TL Switches through all Transmitted Light contrast methods Increases the brightness (Transmitted Light)

  • Page 96: Abbreviation Glossary

    – ABBREVIATION GLOSSARY 9. APPENDIX Abbreviation glossary Adaptive Focus Control Aperture diaphragm Brightfield COMBI Combination methods CUBE Fluorescence cube Darkfield incident / Transmitted Light Differential Interference Contrast ExMan Excitation manager Field diaphragm Fluorescence intensity manager FLUO/Fluor Fluorescence axis (incident light) Interference contrast, incident light Interference contrast, Transmitted Light Internal filter wheel...

  • Page 97: Overview Of Touch Screen Pictograms

    – OVERVIEW OF TOUCHSCREEN MENUS 9. APPENDIX Overview of Touch Screen pictograms Observation ports Navigation bar 100% eyepiece Basic microscope settings 100% camera Contrast method Magnification Eyepiece/camera splitting Stage and focus controls 100% to lower, left or right port Touch screen settings Top port: eyepiece/camera splitting Menus Lower port/left or right port split...

  • Page 98: Additional Information

    9. APPENDIX Additional information General microscopy Objectives For information on the objectives such as the objective Refer to the Leica Science Lab classes and the marking of objectives, refer to http://www.leica-microsystems.com/science-lab/ http://www.leica-microsystems.com/de/produkte/objektive/ to find additional useful information and instructions on the microscope methods.

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