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Nanonics Imaging MultiView 4000 User Manual

Hybrid controller with nws software
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MultiView 4000™
User Guide
For the Hybrid Controller with NWS software
March 2013
Version 6

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Summary of Contents for Nanonics Imaging MultiView 4000

  • Page 1 MultiView 4000™ User Guide For the Hybrid Controller with NWS software March 2013 Version 6...
  • Page 2 Published 2010 Notice Information in this document is subject to change without notice. Nanonics Imaging Ltd. assumes no responsibility for any errors that may appear in this manual. Companies, names and data used in examples herein are fictitious unless otherwise noted. No part of this...

  • Page 3: Table Of Contents

    The Standard Hardware Set-up ............... 11 Additional Hardware for NSOM Measurements ..........14 The Two-Tower Set-up ................... 15 A Closer Look ....................16 The MultiView 4000 Tip-Mount Assembly ............ 19 Probe Parameters ..................... 20 The Integra Controller System ..................22 SPMH Controller ..................... 22 High-Voltage Piezo Driver ................
  • Page 4 MultiView 4000™ User Guide Laser Safety Adjusting the Height of the Upper Microscope ..........49 Preparing the Sample & Tips ..................51 Preparing the Sample ..................51 Placing the Sample ..................51 Mounting the Tip ..................... 51 Mounting Two Probes ..................54 Aligning Two Probes ..................
  • Page 5 MultiView 4000™ User Guide Laser Safety 16.3 Installation ..................... 102...

  • Page 6: Laser Safety

    MultiView 4000™ User Guide Laser Safety 1 Laser Safety HEALTH & SAFETY ADVISORY: 1. It is mandatory to read this section before operating Nanonics MultiView™ products. 2. Always wear the appropriate laser safety goggles as designated by the wavelength or wavelengths being used.

  • Page 7: Eye Protection

    MultiView 4000™ User Guide Laser Safety Eye Protection  1. If the eye is exposed to laser beams the damage may not be immediately noticed. In addition, damage to the eye can be cumulative as a result of repeated exposure.

  • Page 8: Introduction

    MultiView 4000™ User Guide Introduction 2 Introduction The MultiView 4000 is a multi-probe AFM, NSOM and SPM system, consisting of up to four probes. It has an open architecture which allows for flexible integrations with different analytical tools. The modular design of the MultiView 4000 allows for the following combinations: ...

  • Page 9: Optical & Other Complementary Integration

    Optical & Other Complementary Integration The MultiView 4000 system has a number of advantages in the area of optical microscopy and it can also be integrated with other equipment, as described below:  Full integration with upright, inverted or dual (4 Pi) optical microscopes.

  • Page 10: Probe Solutions

    Environmental Control 2.4.1 The MultiView 4000 can be placed inside an environmental chamber (shown below). This allows SPM scanning to be performed in conjunction with confocal microscopy under controlled conditions. The chamber allows you to have precise control over humidity,...

  • Page 11: The Multiview 4000 System

    MultiView 4000™ User Guide The MultiView 4000 System 3 The MultiView 4000 System The Standard Hardware Set-up A typical two-tower MultiView 4000 System is comprised of the following equipment:  MultiView 4000 Tower (2 units)  Dual Microscope  SPMH Controller* (2) ...
  • Page 12 BX51 microscopes. Other host microscopes are also compatible with the system. The microscopes are installed on the Nanonics dual frame stand and the MultiView 4000 is placed on a custom-made interface plate. Dimensions: 50 x 50 x 80 cm SPMH Controller p. 22 Controls the scan and feedback mechanism;...
  • Page 13 MultiView 4000™ User Guide The MultiView 4000 System Stepper Motor Driver p. 34 Provides signals for the lateral stepper motors. It is controlled by the SMC software and interfaces with the NI card installed on the computer. Computer Nanonics supplies a desktop computer with two DT cards, an NI card and the NWS software.

  • Page 14: Additional Hardware For Nsom Measurements

    MultiView 4000™ User Guide The MultiView 4000 System Additional Hardware for NSOM Measurements The following equipment is required for carrying out NSOM measurements. NSOM scanning is described on page 94. Counter & Power Supply p. 36 The APD (Avalanche Photo Diode) Counter &...

  • Page 15: The Two-Tower Set-Up

    The Two-Tower Set-up The two-tower MultiView 4000 system on a dual microscope is shown below. Figure 3: The MultiView 4000 System with two probes and integrated Dual Microscope A complete two-tower MultiView 4000 system scheme appears below: Figure 4: MultiView 4000 - Scheme for a Two- probe System...

  • Page 16: A Closer Look

    The MultiView 4000 Head 3.4.1 A schema of the MultiView 4000 head in a two tower configuration appears below: Figure 5: MultiView 4000 Head Assembly Each tower contains a tip-scanning stage, a tip mount, a pre-amplifier, a Z stepper motor, XY stepper motors and electrical connectors.
  • Page 17 MultiView 4000™ User Guide The MultiView 4000 System MultiView 4000 Table 3.4.2 MultiView 4000 Tower 3.4.3 The parts of the MultiView 4000 tower are shown in the diagrams below. Figure 6: MV4000 Tower Assembly...
  • Page 18 MultiView 4000™ User Guide The MultiView 4000 System Upper Scanner 3.4.4 Each tower houses an upper flat-scanner, as shown below. Figure 7: MV4000 Upper Scanner Electroblock Unit 3.4.5 Figure 8: MV4000 Electroblock Unit The electroblock unit includes:  Tip Mount (see next) ...

  • Page 19: The Multiview 4000 Tip-Mount Assembly

    The MultiView 4000 System Tip Mount 3.4.6 The MultiView 4000 tip mount is shown below. Figure 9: MV4000 Tip Mount The MultiView 4000 Tip-Mount Assembly As shown above, the tip-mount attaches to the electroblock via an electrode panel at its back, and tapers at its front, where a tuning fork is mounted, to which the AFM probe is attached.

  • Page 20: Probe Parameters

    MultiView 4000™ User Guide The MultiView 4000 System The two tines of the tuning fork are connected via wires, indicated by red arrows in Figure 10, to the two outer electrodes on the tip-mount electrode pad. These electrodes transmit the signals generated by the tuning fork response.
  • Page 21 MultiView 4000™ User Guide The MultiView 4000 System This value changes after mounting the tip due to the mass change and due to the mechanical properties of the added glue. Q Factor: The Q factor (Quality Factor) compares the time constant for decay of an oscillating physical system’s amplitude to its oscillation period.

  • Page 22: The Integra Controller System

    MultiView 4000™ User Guide The Integra Controller System 4 The Integra Controller System The Integra Controller system is comprised of the SPM Controller, the High Voltage Piezo Driver, the Low-Voltage Adaptor, the DT Interface, the System Power Supply and the Multi-Channel Stepper Motor Driver.
  • Page 23 MultiView 4000™ User Guide The Integra Controller System Control Description And Settings (in bold) Mirrors Determines the polarity of the output signal from PSD. For the MV4000 this is always set to the “One” position. Monitor Turn the dial to “DC-In”. The monitored signal can be accessed through the “Monitor”...
  • Page 24 MultiView 4000™ User Guide The Integra Controller System Table 3 SPM Rear Panel - Controller Ports Controller Port Function Monitor Connects to the “Height1 Out” BNC on DT Interface via a BNC cable and provides the height signal to the FPGA.

  • Page 25: High-Voltage Piezo Driver

    MultiView 4000™ User Guide The Integra Controller System Lock-in Port Function To PC The DT Interface box is connected to this port to provide software control of both the controller card and the lock-in control. SPM 2 Connects to SPM 1. This connects the Controller ports to the Lock-In ports.
  • Page 26 MultiView 4000™ User Guide The Integra Controller System Scan & Slope Compensation 4.2.1.3 Scan & Slope Compensation mode is the mode normally used when scanning. This mode allows for Hardware Compensation of sample or scanner tilt encountered during a scan by providing linear voltage offset to the Z-channel.
  • Page 27 MultiView 4000™ User Guide The Integra Controller System Control Description Scan Mode Allows a choice of voltage mode to be applied to scanner: Slope-compensation: Used to correct Z-tilt during  a scan. The offset is provided only on the Z-axis.
  • Page 28 MultiView 4000™ User Guide The Integra Controller System Control Description setting high-voltage ranges for Z Divider for Z Range Divider scanning axis 1:1 . Used for samples with high topographic  variations (from hundreds of nanometers to microns) where it may be necessary to make use of the full Z range).
  • Page 29 MultiView 4000™ User Guide The Integra Controller System Rear Panel 4.2.3 Figure 17: HV Piezo Driver - Rear Panel Specifications 4.2.4 The High Voltage Piezo Driver can be driven by either an internal or a variable external voltage source. It provides three bipolar outputs of ±145 V with currents of up to 1 mA, high stability and low noise output voltages.

  • Page 30: Low Voltage Adaptor

    MultiView 4000™ User Guide The Integra Controller System Low Voltage Adaptor Figure 18: Low Voltage Adapter - Front Panel The Low Voltage Adaptor is used to select which scanners will be used for a scan on the Z- and X-Y axes. See Table 7 below for available scanning modes.
  • Page 31 MultiView 4000™ User Guide The Integra Controller System Front Panel 4.4.1 Figure 19: DT Front Panel Table 8 DT Front Panel - Connectors Port Description Slave Connects Master and Slave Digital DT Interfaces. This connection is used when 2 tower are connected to the same FPGA card.

  • Page 32: System Power Supply

    MultiView 4000™ User Guide The Integra Controller System AUX 1 Analog input port (-10 to +10v). Monitor Outputs analog signal correlating to monitor knob setting on front panel of SPM controller. Delayed Trigger Outputs Trigger signal on each substep during the scan Rear Panel 4.4.2...

  • Page 33: Computer

    MultiView 4000™ User Guide The Integra Controller System WARNINGS: 1. Ensure that the correct voltage is selected for your location. 2. Make sure the System Power Supply is off before connecting any of the devices. 3. Do not turn the System Power Supply ON or OFF until 3 to 5 minutes have passed since previous on/off action.

  • Page 34: Stepper Motor Driver

    The Integra Controller System Stepper Motor Driver The MultiView 4000 system uses a number of stepper motors to move the upper scanners (on the towers) and the sample stage. The Multi-channel Stepper Motor Driver (SMD), together with the Stepper Motor Control software, control the stepper motors.
  • Page 35 MultiView 4000™ User Guide The Integra Controller System The following table describes the SMD LEDs. Table 10: LEDs on the Stepper Motor Driver Front Panel SMD Indicator Description Station 1 These LEDs relate to the XY movement of Tower I.

  • Page 36: Counter & Power Supply

    MultiView 4000™ User Guide The Integra Controller System SMD Connector Description SM5…SM7 25-pin connector for sending the commands to the sample stage. This connects 9-pin connectors on the sample stage. SM1…SM4 25-pin connector for sending the commands to Towers I and II. A split cable connects to the 15-pin ports on Towers I and II respectively.
  • Page 37 MultiView 4000™ User Guide The Integra Controller System Port/Control Description Measurement Select the signal to be displayed: Out: Monitors the output frequency, which can be up to 16 MHz. “Out” is the usual setting for this knob. See “Display” below for extra information on reading the value that is displayed in the APD Counter &...
  • Page 38 MultiView 4000™ User Guide The Integra Controller System Port/Control Description Power Terminals Connectors for the APD supply voltage to the APD (red connector, the black connector is ground). The cable ending with the dashed lines goes to the red connector.
  • Page 39 MultiView 4000™ User Guide The Integra Controller System Specifications 4.7.1 Table 13: APD Counter/Power Supply - Specifications Parameter Specification Output Voltage 5.15 V ± 2% Maximum output 1.9 A current Frequency range 10 Hz – 16 MHz counter Sensitivity ranges 1000;...

  • Page 40: Multiview 4000 Connectors

    MultiView 4000™ User Guide MultiView 4000 Connectors 5 MultiView 4000 Connectors This section describes the connectors in the MultiView 4000 system. The Tower Connectors Figure 26: The MultiView 4000 Tower Each tower has four connectors:  Z stepper motor connector: (9 pin) Connects to the SPM controller (To Head). It moves the Z stepper motor up and down allowing the tip to approach the sample surface.

  • Page 41: Sample Stage

    MultiView 4000™ User Guide MultiView 4000 Connectors Sample Stage Figure 27: The Sample Stage The sample stage has a single connector:  Scanner connector: (9 pin) This connector transmits the high voltage signals to the X, Y and Z axes of the scanner. It connects either to the High Voltage Adaptor...

  • Page 42: Tip/Sample Scanning Tower Schema

    MultiView 4000™ User Guide MultiView 4000 Connectors Tip/Sample Scanning Tower Schema Figure 28: Tip/Sample Scanning Tower Schema...

  • Page 43: Tip Scanning Tower Schema

    MultiView 4000™ User Guide MultiView 4000 Connectors Tip Scanning Tower Schema Figure 29): Single Tower Schema with one HV Piezo Driver w/o sample scanner...

  • Page 44: Two Towers Connection Schema

    MultiView 4000™ User Guide MultiView 4000 Connectors Two Towers Connection Schema Figure 30: Tip/Sample Scanning Tower Schema...

  • Page 45: Scanning Modes

    MultiView 4000™ User Guide Scanning Modes 6 Scanning Modes Single Tower The following scanning modes are possible with the sample scanner and a single tower configuration:  Full sample scanning: All X, Y and Z movement is carried out through the lower scanner.

  • Page 46: The Dual Microscope

    Nanonics MultiView systems are designed to integrate fully with this dual configuration: the MultiView 4000 head can be placed in between the upright and inverted microscopes with full view of the Z axis from the top and bottom. This allows for multi-mode NSOM operations such as transmission and reflection.

  • Page 47: Opening And Closing The Dual Microscope

    MultiView 4000™ User Guide The Dual Microscope Opening and Closing the Dual Microscope To open the microscope >> 1. Loosen the holding screw of the upper microscope. 2. Switch off the magnet in order to release the upper turret. 3. Gently push the upper microscope around the pole in order to gain access to the interface plate.
  • Page 48 MultiView 4000™ User Guide The Dual Microscope To align the objectives >> 1. Place a sheet of plain white paper on the interface plate, in the sample position. 2. Turn on the illumination spots of the two microscopes. 3. For coarse alignment of the two microscopes, release both magnets, both holding screws, and rotate one or both microscopes around their axes until the two illumination spots visually coincide.

  • Page 49: Focusing The Microscopes

    MultiView 4000™ User Guide The Dual Microscope Focusing the Microscopes The holding magnets restrict free movement of the microscope optics and allow for fine focusing in the micron range, using the focusing knobs. To carry out coarse focusing in the centimeter range, turn off the holding magnets in order to release the turret.
  • Page 50 MultiView 4000™ User Guide The Dual Microscope Tighten the holding screw. Loosen the support clasp using the M8 metric key. Lower the support clasp to the desired position and tighten it. Loosen the holding screw, and while holding the microscope with both hands lower the screw and microscope to the support clasp.

  • Page 51: Preparing The Sample & Tips

    MultiView 4000™ User Guide Preparing the Sample & Tips 8 Preparing the Sample & Tips Preparing the Sample >> To prepare the sample Select a sample mount. There are several versions of the sample mount supplied with the MV4000 – a flat sample mount, and several with elevated rims of differing height. These mounts allow variable gross Z-positioning depending upon sample thickness and the measurement to be made.
  • Page 52 32. The tip parameters are listed on the back of the tip mount box, as depicted in Figure 33 below. Refer to Section Probe Parameters, page 20, for tip structure and geometry. Figure 32: MultiView 4000 AFM tip mounts in case Figure 33: Back of AFM tipmount case, showing tip parameters The tip mount must be installed on the upper scanner.
  • Page 53 MultiView 4000™ User Guide Preparing the Sample & Tips Follow the guidelines below when mounting the tips, as they are extremely delicate. Contact between the tip and the sample can damage the tip and render it unusable. Before installing the tip mount, rotate the tower(s) away from the lower scanner. This is to prevent the tip from coming into contact with the sample or the lower scanner and causing damage.

  • Page 54: Mounting Two Probes

    MultiView 4000™ User Guide Preparing the Sample & Tips using the Z-stepper motor up to a safe Z distance, as explained above (.Clearing the Working Area around Upper Scanner). After installing the tip mount, rotate the tower back to its initial position.
  • Page 55 MultiView 4000™ User Guide Preparing the Sample & Tips When the tips are close to each other, use the 50x objective in order to carefully monitor the distance between the tips. The fine adjustment of the two tips’ distance has to be done by supplying offset voltages through the High Voltage Piezo Driver to move the respective upper scanners.

  • Page 56: Powering Up The System

    MultiView 4000™ User Guide Powering Up the System 9 Powering Up the System It is very important to follow carefully the steps below when powering up the MV 4000 MultiView system. Failure to follow the instructions may well result in inaccurate measurements and even could damage the system.
  • Page 57 MultiView 4000™ User Guide Powering Up the System In this case check the connection between the computers (see NWS Software manual) The NWS main window will open. Figure 35: NWS Software Main Window ...

  • Page 58: Phase Feedback Settings

    MultiView 4000™ User Guide Phase Feedback Settings 10 Phase Feedback Settings This section explains how to configure the Nanoworkshop (NWS) software to carry out an AFM scan using Phase Feedback. Phase Feedback is the default setting in the NWS software. For an overview of the NWS software and its capabilities, see the NWS User' Manual.
  • Page 59 MultiView 4000™ User Guide Phase Feedback Settings Click LockIn, on the left, to open the Lock-in Controller. The LockIn Controller window opens in the bottom half of the screen. Figure 36: The Lock-in Controller Setting the Total Gain and Oscillation Amplitude 10.2.2...
  • Page 60 MultiView 4000™ User Guide Phase Feedback Settings Setting Feedback Mode 10.2.3 Overview: There are two Feedback modes available on a Nanonics SPM – Phase Feedback and Amplitude Feedback. In Phase Feedback, the Error signal is "recorded" according to the changes of the tuning fork phase. Phase feedback has been found to be much more sensitive than the amplitude feedback.
  • Page 61 MultiView 4000™ User Guide Phase Feedback Settings Setting Sweep Frequency 10.2.4 >> To set the Sweep Frequency Set the minimum (From:) and ,maximum (To:) values for the frequency range to be swept. The range should be chosen according to the resonance frequency of the probe being used.
  • Page 62 MultiView 4000™ User Guide Phase Feedback Settings Figure 38: Sweep Graph Window Move the yellow cursors on the left and right side of the displayed curve to zoom in on the curve's peak. Alternatively, insert frequency values in From and To on the right panel.
  • Page 63 MultiView 4000™ User Guide Phase Feedback Settings  If the curve peak exceeds 8 V. reduce the 'Oscillation Out 1' value and repeat steps 1 and steps 2 – 4 as needed.  If the curve peak is less than 8 V., increase the 'Oscillation Out 1' value and repeat steps 1 and 2 –...
  • Page 64 MultiView 4000™ User Guide Phase Feedback Settings Figure 40 Graph Display upon completion of Autophasing Generating the Phase Graph 10.2.7 To generate the Phase Graph >> On the center panel, click Sweep Signal and select Squared in the pop-up menu (Figure 41).
  • Page 65 MultiView 4000™ User Guide Phase Feedback Settings Figure 42: Obtaining the Phase Curve Move the red vertical cursor along the X axis (the frequency axis) so that it intersects the graph at the vertical center of the curve (see Figure 42 above.).
  • Page 66 MultiView 4000™ User Guide Phase Feedback Settings NOTE: This adjustment should only be carried out once Advanced settings tab 10.2.8 The signal from the tuning fork is sent to a preamplifier and then to the Ext-In Input of the Lock-in board. Hence, in Advanced Settings tab, always use the Ext-In option for the MV4000.
  • Page 67 MultiView 4000™ User Guide Phase Feedback Settings Q factor 10.2.10 Use the ‘Calculate Q factor’ option for checking the Q factor of the probe that is mounted. Q factor calculation is performed through the Phase graph. Use the same procedure as in 10.2.7 to generate the phase graph.
  • Page 68 MultiView 4000™ User Guide Phase Feedback Settings Figure 44: Adjusting the Setpoint in the NWS Software >> To locate the Setpoint Click the SPM Control tab. Enter “0” in the Setpoint field. From the Read Channel drop down list, select “Error”.

  • Page 69: Amplitude Feedback Settings

    MultiView 4000™ User Guide Amplitude Feedback Settings 11 Amplitude Feedback Settings This section explains how to configure the Nanoworkshop (NWS) software to carry out an AFM scan using Amplitude Feedback. Amplitude Feedback uses tuning fork amplitude changes to generate the Error signal for feedback. The default setting in the NWS software should be set to Phase Feedback.
  • Page 70 MultiView 4000™ User Guide Amplitude Feedback Settings Figure 45: The Lock-in Controller Setting the Total Gain and Oscillation Amplitude 11.2.2 ‎ Please refer to 1 0.2.2 for an overview of this section To set the Total Gain and Oscillation Amplitude >>...
  • Page 71 MultiView 4000™ User Guide Amplitude Feedback Settings 2. In Sweep Signal, select Magnitude. 3. In Averaging, set value to 1. (Averaging sets the displayed curve averaging for each point on the swept signal. The available range is 1 – 5).
  • Page 72 MultiView 4000™ User Guide Amplitude Feedback Settings value, and To: to 1 kHz above that value. For example, if the listed value for a probe's resonance frequency (f.) is 32 kHz, then From: should be set to 31 kHz and To: should be set to 33 kHz.
  • Page 73 MultiView 4000™ User Guide Amplitude Feedback Settings Figure 47: Sweep Graph Window Move the yellow cursors on the left and right side of the displayed curve to zoom in on the curve's peak. Alternatively, insert frequency values in From and To on the right panel.
  • Page 74 MultiView 4000™ User Guide Amplitude Feedback Settings  If the curve peak is less than 8 V, increase the ‘Oscillation Out 1’ value and repeat steps 1 and 2 – 4 as needed. NOTE: In some cases, the resonance curve is asymmetrical or has very low amplitude. This is often caused by improper tip mounting, resulting in poor electrode contact.
  • Page 75 MultiView 4000™ User Guide Amplitude Feedback Settings Figure 49: Graph Display upon completion of Autophasing Click Finish, on the lower right, to exit the LockIn controller. Advanced Settings tab. 11.2.7 Please refer to section ‎ 1 0.2.8 in the Phase Feedback chapter. The advanced settings for Amplitude Feedback are the same as for Phase Feedback.
  • Page 76 MultiView 4000™ User Guide Amplitude Feedback Settings Figure 50: Adjusting the Setpoint in the NWS Software >> To locate the Setpoint 1. Click the SPM Control tab. 2. Enter “0” in the Setpoint field. 3. From the Read Channel drop down list, select “Error”.

  • Page 77: Carrying Out An Afm Scan

    Carrying Out an AFM Scan 12 Carrying Out an AFM Scan When you have configured the MultiView 4000 system and NWS software for Phase (p. 56) or Amplitude Feedback (p. 69) scanning, you are ready to carry out the actual AFM scan, as described below.

  • Page 78: Setting The Feedback Gains

    MultiView 4000™ User Guide Carrying Out an AFM Scan Figure 51: Default Divider Values Figure 52: Z Divider Values for Piezo Versions Setting the Feedback Gains 12.2 The Feedback system of the Hybrid controller is based on 2 gains – Proportional and Integrator.
  • Page 79 MultiView 4000™ User Guide Carrying Out an AFM Scan Figure 53: Setting the Feedback Gains Gains adjustment. The algorithm for feedback gains adjustment is quite simple. There are 2 main criteria for properly adjusted gains: trace and retrace topography (Height) curves matching and a minimization of the Error signal.
  • Page 80 MultiView 4000™ User Guide Carrying Out an AFM Scan Figure 55: Error profile of the trench. The saturation happens at the level of the Error signal, which is set before approach. In order to increase the feedback reaction speed we need to increase the proportional gain.

  • Page 81: Approaching The Sample Surface

    MultiView 4000™ User Guide Carrying Out an AFM Scan Approaching the Sample Surface 12.3 >> To approach the sample surface  In the SPM Control tab, click Approach. The stepper motor brings the upper scanner, with the tip toward the sample on the lower scanner and stops when feedback is established.
  • Page 82 MultiView 4000™ User Guide Carrying Out an AFM Scan Figure 56: FB ON Indicator on SPM Figure 57: Feedback Indicator on Controller NWS Software A Closer Look at the Approach Process: When you click Approach, as directed above, the engagement of the tip with the surface is always accomplished using a Safe Approach mode.

  • Page 83: Setting The Scan Parameters

    MultiView 4000™ User Guide Carrying Out an AFM Scan Figure 58: Safe Approach Parameters on the Miscellaneous Settings Pane Setting the Scan Parameters 12.5 To set the scan parameters >> On the Actions & Settings upper tab, select the lower Scan Window tab.
  • Page 84 MultiView 4000™ User Guide Carrying Out an AFM Scan Explaining the Parameters on the Scan Window Tab  Fast Axis refers to the direction of scan (see below).  Slow Axis refers to the perpendicular direction.  Speed can be set to values normally from 8ms to 12ms.

  • Page 85: Starting The Scan

    MultiView 4000™ User Guide Carrying Out an AFM Scan  To choose different lengths for the X and Y axes, clear the Force Square check box (under Scan Parameters) and adjust the settings in the Window Size fields. Starting the Scan 12.6...
  • Page 86 MultiView 4000™ User Guide Carrying Out an AFM Scan  You can adjust the setpoint using the slider between the two scopes (see Figure 61 below), or by entering a value in the numerical field and pressing the Tab key. You can also change the setpoint in the SPM Control tab during the scan.

  • Page 87: Adjusting The Tilt

    MultiView 4000™ User Guide Carrying Out an AFM Scan  Use ‘Subtract Linear Fit’ to display the data in the ‘upper scope window’ with linear fit subtraction. This option is useful when small features are present on a large background and the user wants to observe only the small features.

  • Page 88: Saving A Scanned Image

    MultiView 4000™ User Guide Carrying Out an AFM Scan To stop the scan >>  In the Status Indicator area at the top of the NWS screen, click Stop Scan. When a scan has been stopped you cannot restart it but you can save it, as explained below.

  • Page 89: Retracting The Probe

    MultiView 4000™ User Guide Carrying Out an AFM Scan Figure 62: The Files Tab To save in WSxM format >> When you save the image in the WSxM format you can use it in the WSxM image processing software. On the Files tab, click the WSxM tab.
  • Page 90 MultiView 4000™ User Guide Carrying Out an AFM Scan 1. If the “Retract tip after scan” option was selected then the tip will be retracted automatically when the scan has finished. The option is located in Actions & Settings >...

  • Page 91: Feedback Gains

    MultiView 4000™ User Guide Feedback Gains 13 Feedback Gains The Function and Importance of PID Gains 13.1 in the AFM Feedback Loop The settings of the three feedback gains are very important to ensure a proper feedback for preventing tip crash or removal of the fibers. These gains operate directly on the “Error”...
  • Page 92 MultiView 4000™ User Guide Feedback Gains The Integrator (I) Gain 13.2.2 The Integrator gain sets the integration time or the time response of the scanner. That is, it sets the time for the scanner to respond for any change of the Error signal. The larger the integrator gain, the faster the scanner responds.

  • Page 93: Summary: An Afm Checklist

    MultiView 4000™ User Guide Summary: An AFM Checklist 14 Summary: An AFM Checklist Here is a summary of all the steps explained in detail in the preceding sections. Use this summary as a checklist to ensure you haven't omitted anything as you become more familiar with the various procedures.

  • Page 94: Nsom Scanning

    MultiView 4000™ User Guide NSOM Scanning 15 NSOM Scanning This section describes the following topics:  Connecting a Detector, below.  Mounting the NSOM Probe, p. 95.  Preparing the NSOM Probe, p. 96.  Positioning the Tip, p. 98.

  • Page 95: Mounting An Nsom Probe

    However, due to the NSOM probe’s tail, note the points below. The NSOM tip is shown in its case below. The tail is wound in a coil. Figure 69: The MultiView 4000 NSOM tip When you have moved the towers away from the lower scanner and raised the upper scanner, continue below.

  • Page 96: Preparing The Nsom Probe

    MultiView 4000™ User Guide NSOM Scanning Make sure that the fiber tail passes through the groove on the upper scanner (underneath the plate). Tighten the fixing screw to create electrical contact between the upper scanner and the tip mount. If necessary, place the second tip mount on the other upper plate.
  • Page 97 MultiView 4000™ User Guide NSOM Scanning 5. Press down on the cleaver Do not pull the fiber in order to snap it. 6. Bend the rubber base of the cleaver in order to snap the fiber. 7. The stripped part of the fiber will now be approximately 2 mm long.

  • Page 98: Positioning The Tip

    MultiView 4000™ User Guide NSOM Scanning 3. Make sure that the stripped end of the fiber is at the focus of the objective lens. This should be 2 mm away from the objective lens, so that the fiber will be in the area of the most concentrated light.

  • Page 99: Carrying Out An Nsom Scan

    MultiView 4000™ User Guide NSOM Scanning If the monitor shows a zero reading then: (iv) Check that the APD Counter & Power Supply cable is connected properly to the Detector. See page 94. Check that the power supply gives a 5 V reading by moving the Measurement knob to “Power”.
  • Page 100 MultiView 4000™ User Guide NSOM Scanning Figure 71: APD Counter and Power Figure 70: APD Amp (Software) Supply (Hardware) If the signal is in saturation, change the range of the APD/PMT counter (or misalign the APD/PMT) and restart the scan.

  • Page 101: Appendix: The Vibration Isolation Table (Minus K Table)

    MultiView 4000™ User Guide Appendix: The Vibration Isolation Table (Minus K Table) 16 Appendix: The Vibration Isolation Table (Minus K Table) Overview 16.1 The Minus K Vibration Isolation Table (model 150BM-1) is an integral element of the MV systems. The Minus K table enables vibration-free operation of the system in a wide range of situations.
  • Page 102 MultiView 4000™ User Guide Appendix: The Vibration Isolation Table (Minus K Table) Installation 16.3 Attaching Table Legs 16.3.1 The Minus K vibration isolation table is supplied with three legs. These legs will help to adjust the height of the table as well as to insure that it is perfectly level. These legs are threaded and screw into three base plates on the underside of the table.
  • Page 103 MultiView 4000™ User Guide Appendix: The Vibration Isolation Table (Minus K Table) The plate should now rest centered just above the base. 2 . Place the four square pads, found between the plate and the base, in the four corners between the plate and the base.
  • Page 104 MultiView 4000™ User Guide Appendix: The Vibration Isolation Table (Minus K Table) Figure 77: Rubber Pads on Table Plate 2. Then place the breadboard (Figure 78, below), centered, on the table plate. Figure 78: Breadboard Leveling the Table 16.3.4 Leveling the table is done by adjusting the height of the three legs using an open key wrench.
  • Page 105 MultiView 4000™ User Guide Appendix: The Vibration Isolation Table (Minus K Table) Figure 79: Adjusting Leg Height Figure 80: Level Indicator Setting Up the Microscope on the Table 16.3.5 Figure 81: MV4000 on Minus K Table Please note that the microscope apparatus is rather heavy (see Figure 81, above). It is much preferable that two people transport and carefully set the microscope on the breadboard.
  • Page 106 MultiView 4000™ User Guide Appendix: The Vibration Isolation Table (Minus K Table) Figure 82: Cable Management System 1. Connect the Cable Management System (Figure 82, above) to the breadboard. 2 . Thread the cables through the Cable Management System and close it.
  • Page 107 MultiView 4000™ User Guide Appendix: The Vibration Isolation Table (Minus K Table) Table 14: Minus K Table Load Range Load Adjustment 16.3.6 1. Adjust the load of the Minus K table using the handle on the front panel. a) If the position indicator is too high, decrease the load setting by turning the handle counter clockwise.
  • Page 108 MultiView 4000™ User Guide Appendix: The Vibration Isolation Table (Minus K Table) The vibration frequency should be between ½ to 1 Hz. 2. To confirm that during this movement the plate is not touching and of the side walls of the base., gently push the Minus K plate forward and try to feel if it touches any of the walls.
  • Page 110 When contacting the Nanonics Support Dept. please indicate the following: 1. Name of company/university 2. MultiView product 3. Software (NWS or Quartz) Shipping Address: Nanonics Imaging Support Dept., Manhat Technology Park, Building 1 Floor 2, Malcha, Jerusalem 91487, Israel Tel: +972-2-678 9573 Fax: +972-2-648 0827 E-mail: support@nanonics.co.il...

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