Page 1 — ABB MEASUREMENT & ANALYTICS | USER MANUAL User Manual | ICOS GLA132 Ultraportable Liquid Water Isotopic Analyzer...
Page 2 Only trained service personnel are authorized to open the housing or service the laser. Using this analyzer in a manner not specified by ABB-LGR may result in damage to the analyzer, and render it unsafe to operate. Only authorized persons may open the analyzer cover or perform internal maintenance.
Page 3: Table Of Contents
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Table of Contents Disclaimer ............................7 Cybersecurity ..........................7 Patent .............................. 7 Copyright ............................7 Safety .............................. 8 Class of Laser Equipment ......................8 Certification ..........................8 WEEE Directive ........................... 8 Labels ............................
Page 4 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 The PAL3 LSI Autoinjector ......................29 Unpack the Autoinjector ......................30 Assemble the Autoinjector ....................31 Install the Septum ........................38 Function Keys ........................... 39 Adjust Software Settings ...................... 40 Install the Syringe ........................
Page 5 Injected Volume Correction ..................145 Appendix B: About Gas Analyzers and Laser Absorption Spectroscopy ......149 Conventional Laser Absorption Spectroscopy ..............149 ABB-LGR’s Off-Axis Integrated-Cavity Output Spectroscopy (Off-Axis ICOS) ....151 Appendix C: Accessing Data Using the Ethernet ..............152 Additional Notes ........................153...
Page 6 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Appendix I: Cables ........................... 228...
Page 7: Disclaimer
ABB and its affiliates are not liable for damages and/or losses related to such security breaches, any unauthorized access, interference, intrusion, leakage and/or theft of data or information.
Page 8: Safety
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Safety The following pages provide important safety precautions. Class of Laser Equipment The analyzer is a Class 1 laser instrument when the case cover is closed for normal operation, and the lock is installed. Certification The analyzer certifications are listed in Table 1.
Page 9: Labels
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Labels The following labels are at specific locations on or in the analyzer to identify hazardous areas. (Figure 1) Figure 1: Radiation Labels These labels are located on the enclosure covering the ICOS cell. The fiber laser is visible only when the insulated enclosure is removed from the ICOS cell.
Page 10: Laser Hazards
Only trained service personnel are authorized to open the housing or service the laser. Using this analyzer in a manner not specified by ABB-LGR may result in damage to the analyzer and render it unsafe to operate. Only authorized persons may open the analyzer cover or perform internal maintenance.
Page 11: Text Formats And Warning Icons
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Text Formats and Warning Icons Text Formats This section describes text formats and warning icons used in this manual. Italicized text is used for emphasis in text and also to emphasize the names •...
Page 12: Transportation And Storage Of Boxed Analyzers
Save the original shipping materials to use when returning the analyzer to ABB-LGR if factory service or repair is needed. Table 3 lists and describes the safety icons on ABB-LGR shipping boxes. Follow these instructions when transporting and storing boxed analyzers.
Page 13: Warranty
5. No-charge repair parts may be sent at ABB-LGR’s sole discretion to the purchaser for installation by purchaser. 6. ABB-LGR’s liability is limited to repair or replace any part of the analyzer free of charge if ABB-LGR’s examination discloses the part to be defective.
Page 14: Warranty & Returns
• The company-issued RMA number must be prominently displayed on the return package. No returns will be accepted collect or C.O.D. On all warranty returns, ABB-LGR will pay the shipping charges on the return of the merchandise to the customer.
Page 15: Analyzer Overview
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Analyzer Overview The ABB-LGR Ultraportable Liquid Water Isotopic Analyzer measures O and D/H in liquid water samples with high accuracy. An autoinjector enables long-term liquid water isotope monitoring studies without user intervention. Due to its rugged packaging, this analyzer is ideal for a wide variety of hydrological, analytical, and biological science applications that involve measurements of fresh water or sea water.
Page 16: Performance Specifications
• 10 Amps • Fast-blow fuse • Cable Plugs and Voltage for EC Countries See page 228. • Always use the power supply cord provided by ABB-LGR. See page 228 for a description of power cords for a specific country.
Page 17: Standard Components
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Standard Components This section describes the analyzer components. Verify that each of the system components has arrived before installation. GLA132 Liquid Water Series Analyzer Analyzer power cord • User guide (this document) •...
Page 18: Optional Components
Battery power to the analyzer is interrupted • If any of these failures occur, you will need a mouse, keyboard and monitor to restart the analyzer. A back up hard drive is recommended. If you have not received all of these components, contact ABB-LGR at icos.support@ca.abb.com.
Page 19 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Figure 2 shows the left side of the analyzer. Figure 2: Left Panel Figure 3 shows the right side of the analyzer. Figure 3: Right Panel...
Page 20: Power Connections
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Power Connections The power connector is on the left side panel of the analyzer. (Figure 4) The analyzer can be powered two ways: Using the AC/DC power supply to the DC power inlet •...
Page 21: Data Interface Connection Ports
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Data Interface Connection Ports This section describes the data interface connections as shown in Figure 5. These connections vary from analyzer to analyzer depending on the ordered configuration. USB ports – Used for transferring data to a USB memory device, or to connect a •...
Page 22: Plumbing Diagram
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Plumbing Diagram When flushing dry air into the cavity, valve #2 is closed and valve #1 is open. When the analyzer is pumping down, valve #1 is closed and valve #2 is open. When the analyzer is measuring a sample, the cavity is pumped down and stable at ≈1 Torr, and both valves are closed.
Page 23: Inlet/Outlet Connections
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Inlet/Outlet Connections The inlet and outlet ports are located on the left side panel of the analyzer. (Figure 7) The unit ships with inlets and outlets capped for protection. The connections use Swagelok fitting ISO thread sizes 1/8”, 1/4”...
Page 24: Warning Labels And Descriptions
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Warning Labels and Descriptions This section describes the warning labels shown on the analyzer. Table 4 gives a description of the warning labels. • Figure 8 shows the location of the labels on the analyzer. •...
Page 25: Specifications
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Specifications This section provides the weight and dimensions of the analyzer. (Figure 9) External Dimensions 18.5” W x 14” D x 7” H Weight 15 kg Cell Volume 345 cc cavity •...
Page 26: Analyzer Setup
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Analyzer Setup Connect the Power Cords Analyzer • Connect the AC/DC power supply power cord from the power supply to a grounded outlet. Connect the power connector cord on the power supply to the 11-30 VDC port on the left side of the analyzer.
Page 27 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Figure 10: Drierite® Laboratory Gas Drying Unit When using dry gas from a cylinder or a house dry gas system, make sure that the inlet pressure is between 0-5 psig. 3.
Page 28: Attach And Tighten The Swagelok Connectors
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Attach and Tighten the Swagelok Connectors Tighten the Swagelok connections to between 1/4 and 1/2 turn past finger tight. Leave a gap of at least 3.5 mm as shown in Figure 12. 2.
Page 29: The Pal3 Lsi Autoinjector
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 The PAL3 LSI Autoinjector The PAL3 autoinjector allows a throughput of 800 injections per day. It communicates with the analyzer through a local network communication. The PAL3 LSI User Manual contains additional diagrams, configuration data, and function control data to assist you when using the autoinjector with the analyzer.
Page 30: Unpack The Autoinjector
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Unpack the Autoinjector The PAL3 autoinjector is shipped in a box separate from the analyzer. It contains the PALbase, PAL arm, PALterminal, power supply, attached tray holder, attached injector port, trays, safety shield and provided accessories.
Page 31: Assemble The Autoinjector
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Assemble the Autoinjector To assemble the autoinjector: Install the PALhead (vertical arm). a. Using the provided T-25 screwdriver, unscrew the 2 screws on the back side of the autoinjector. Save these screws! (Figure 14) i.
Page 32 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 d. Lift the vertical arm onto the Y-axis by setting the arm on the two locating pins. (Figure 16) Have a second person hold the arm in place while the screws are being inserted.
Page 33 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 g. Re-attach the cover, using the original screw. (Figure 18) Figure 18: Attach the cover 2. Install the Safety Guard. a. Install the provided safety guard brackets onto both sides of the autoinjector.
Page 34 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 b. Attach the safety guard to both brackets. Carefully hold the safety guard in the proper position as you screw it into the brackets. Alternate screwing the safety guard into the left and right side until it is secure. (Figure 20) Figure 20: Attach the safety guard For safety reasons, it is mandatory to install the Safety Guard to the PAL3 autoinjector.
Page 35 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 The keypad and holder can be installed on either side of the autoinjector. b. Insert the display screen into the holder. (Figure 22) Figure 22: Display Screen mounted in holder 4.
Page 36 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 a. Connect the black cord from the POWER port to the autoinjector power supply. (Figure 23 and Figure 24) Figure 24: Electrical Connections to PALrobot b. Connect the grey display screen cord to the TERMINAL port. (Figure 23 and Figure 24) 5.
Page 37 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 The Dynamic Host Configuration Protocol (DHCP) is enabled on the PAL3 autoinjector to automatically assign an IP address to the autoinjector. To enter a unique static IP address, see Enter a Unique Static IP Address on page 201.
Page 38: Install The Septum
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Refer to Connect the Analyzer Inlet/Outlet Connections on page 26 for attaching the transfer tubing from the autoinjector to the analyzer. 10. Turn the autoinjector on by pressing the POWER ON switch on the power supply. 11.
Page 39: Function Keys
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Function Keys The button functions are as follows: Menu Button A (Options) : Options for a particular menu are assigned to the • corresponding function key on the left side. Menu Button B (Default Action) : Reserved for default actions, such as Save, •...
Page 40: Adjust Software Settings
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Adjust Software Settings The autoinjector software is pre-installed. Do NOT change the parameters unless the autoinjector software is not functioning properly. This section details each step: Check the tray holder reference position •...
Page 41 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 d. Scroll to Check Teaching (Figure 29) Figure 29: Check Teaching i. This confirms the x, y and z axes ii. Press Enter iii. The autoinjector will beep and the arm will move to the Teach point. This disk is referred to as a Lunette.
Page 42 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 iv. Verify that the needle guide is centered on the lunette. (Figure 31) Figure 31: Needle guide is centered on the lunette v. Press Next (Button B) vi. Press OK (Button B) to move the PALhead back to the Home position Figure 32: Move PALhead to home position vii.
Page 43 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Check the position of the three vial trays The vial tray parameters are pre-set, using the provided vials and caps. If you use a different style vial and cap, the parameters may need to be reset. Refer to Appendix H: The PAL3 LSI Autoinjector on page 193 for details on how to teach vial positions.
Page 44 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 3. Enter the sequence of commands using the keypad dial wheel and center button as shown in Figure 27. a. On the Home Screen, scroll to Tray Holder 1. Press Enter. (Figure 35) Figure 35: Tray Holder 1 b.
Page 45 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 d. Select Rack 1 . Enter. (Figure 37) Figure 37: Rack 1 e. Press Options (Button A) Scroll to Check Teaching. This will confirm the x, y and z-axes. (Figure Figure 38: Check Teaching...
Page 46 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 g. Press Enter. h. Press Check (Button B). The arm will move to Vial #1 in Tray #1. Confirm that the needle is centered over the vial. The needle guide must be centered over the vial in position 1 to prevent syringe breakage.
Page 47 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Check the Position of the Injection Port Place the provided Syringe Alignment Tool onto the septum cap. (Figure 40) This tool remains on the septum cap during injections to guide the syringe into the proper location.
Page 48 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 3. On the Main Menu , scroll to Injector LC 1. (Figure 41) Figure 41: Injector LC 1 a. Press Enter b. Press Options (Button A) Scroll to “ Check Teaching ”. (Figure 42) i.
Page 49 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 The arm will move to the teach point (TP) of the heated injector block. (Figure 43) Confirm that the black conical syringe guide sits securely within the syringe alignment tool. (Figure 43) Figure 43: Syringe Guide h.
Page 50 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Check the Needle Penetration Depth This step needs to be performed anytime that a new vial type or vial insert is used. Switching vial types can result in syringe breakage. Remove trays #2 and #3 from the Tray Holder .
Page 51 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 d. On the Home Screen, scroll to Tray Holder 1. Press Enter. (Figure 44) Figure 44: Tray Holder 1 e. Scroll to Slot 1. (Figure 45) Figure 45: Slot 1 Press Enter.
Page 52 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 g. Select Rack 1 . (Figure 46) Figure 46: Rack 1 h. Press Options (Button A) Scroll to Check Needle Penetration. (Figure 47) Figure 47: Check Needle Penetration Press Enter. k.
Page 53 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Carefully, turn the dial to insert the needle in millimeter increments until the desired depth has been reached. The user will add this value to the analyzer software in the following steps. Example: 32mm is an appropriate setting for the provided vials.
Page 54: Install The Syringe
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Install the Syringe Manual priming is recommended for 1.2 µL Hamilton syringes before installation. Priming the syringe: Actuate the syringe in deionized water, or NMP syringe lubricant (1-Methyl-2-pyrrolidinone, Sigma Aldrich). If syringe lubricant is used, refer to Caring for Syringes on page 131 for proper use.
Page 55 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 b. Slide the flat side of the syringe into the groove of the upper section of the holder. Position the tip of the needle into the upper and lower needle guide by slowly lowering the syringe.
Page 56 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 d. Push the Plunger Coupling Adapter towards the Syringe Plunger Adapter so that it touches the coupling device. The Plunger Coupling Adapter should connect with the Syringe Plunger . (Figure 53) Figure 53: Lower the Plunger Coupling Adapter 8.
Page 57 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 d. Slowly remove the syringe from the Syringe Adapter by lifting it upwards. (Figure 55) Figure 55: Lift the syringe out of the Syringe Adapter 9. Press Next (Button B) 10.
Page 58: Pair The Autoinjector With The Liquid Water Isotopic Analyzer
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Pair the Autoinjector with the Liquid Water Isotopic Analyzer The PAL3 autoinjector communicates with the Liquid Water Isotopic Analyzer through the provided 5-port Ethernet Switch. (Figure 25) To pair the PAL3 autoinjector with the analyzer: Within the Setup menu on the Liquid Water Isotopic Analyzer, click on the Find auto injector(s) tab.
Page 59: Prepare Samples For Analysis
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Prepare Samples for Analysis To prepare water samples: If the sample contains visible particulates, filter the sample using very fine filter paper (Advantec MFS® grade No. 235 or similar). a. Samples that contain high concentrations of contaminants (for example, volatile organics or proteins) may require additional sample preparation.
Page 60: Prepare Biological Samples For Analysis
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Prepare Biological Samples for Analysis This procedure applies to sample preparation and measurement of plasma, urine and saliva samples for deuterium and O content versus known standards on the TLWIA. The proteins in plasma and saliva samples must be precipitated by zinc sulfate.
Page 61: Initialize And Run The Analyzer
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Initialize and Run the Analyzer To initialize the analyzer: Press the power switch on the left side of the analyzer to the ON position. (0 = OFF / - = ON) The internal computer initializes, and a screen (Figure 58) displays as the program loads.
Page 62: File System Integrity Check
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 File System Integrity Check Once a month, the analyzer automatically performs a file system integrity check following initialization. Figure 59 shows the screen you see while the integrity check runs. The integrity check runs for one to two minutes before launching the analyzer’s control software.
Page 63: The Launch Service Screen
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 The Launch Service Screen The Launch Service screen displays when initialization is complete. From this interface, you can: Bypass the auto launch countdown by clicking LWIA. • Open the auto launch window by clicking Service. •...
Page 64: The Auto Launch Screen
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 The Auto Launch Screen The Auto Launch and Maintenance settings are available when you click the Service button on the Launch Service screen. From this interface, you can: Change the auto launch delay timing. •...
Page 65: Main Panel
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Main Panel After the software launches, the Main Panel is displayed. (Figure 62) This panel contains the User Interface Control Bar (Figure 63) and the Configuration Display . (Figure 64) Figure 62: Main Panel User Interface Control Bar Use the control bar to operate the analyzer.
Page 66 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Display – Toggles through the three Main Panel display formats: Configuration Display – Default display. Displays configuration settings and • available controls to set up analyses. (Figure 64) Spectrum Display – Displays the raw and fitted spectral scans. (Figure 70) •...
Page 67: Main Panel Display Screens
Samples to Measure pane • Standards to Measure pane • The Example Run Configurations section on page 100 shows example configurations created by ABB-LGR that you can use as a starting point to build your own custom configurations. Figure 64: Configuration Display...
Page 68 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Tray Population The Tray Population Pane contains information about the samples and standards to measure. Use this pane to: Assign custom names • Assign trays and positions within the tray •...
Page 69 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Table 6 explains how to use the Tray Population pane. Table 6: Using the Tray Population Pane Task Procedure Available Samples 1. Select the tab. Add samples to population. Add Item 2.
Page 70 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Samples To Measure The Samples To Measure pane lists the items you select as samples for the measurement run. (Figure 66) Figure 66: Samples To Measure Table 7 describes how to use this pane. Table 7: Using the Samples To Measure Pane Task Procedure...
Page 71 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Add item to Samples to Available Samples 1. Select an item from the in the Measure Tray Population pane . 2. Click the upper green arrow To add multiple measurement sets for selected samples, use the spinner control below the green arrow button.
Page 72 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Task Procedure Available Select an item from the Standards Add an item to the Standards Tray Population in the pane. to Measure list. Click the lower green arrow Add multiple injection sets for To add multiple injection sets for selected selected standards.
Page 73 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Additional Run Configuration Actions Use the four highlighted buttons shown in Figure 68 to import, load, save, or set run configurations. Figure 68: Additional Run Configuration Button Controls Table 9 explains how to use these buttons. Table 9: Using Additional Run Configuration Buttons Task Procedure...
Page 74: Run Display
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Run Display Interface Control Bar Run Display . Click the Display button on the User to switch to the Figure 69 The Run Display shows the sequence of injections that were populated in the Configuration Display .
Page 75 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Table 10 describes the columns within the Run Display . Table 10: Run Display Column Fields and Descriptions Column Name Description Name Sample/Standard name Serial number Tray Tray number (1-4) Position of the vial within the tray (1-54) Comment Optional comment added to a...
Page 76 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Within this screen, you can start and stop a configured run. To start a run: Start Click the button. a. The analyzer goes through a flush cycle, filling the measurement cavity with dry air and then purging for approximately one minute to remove residual water.
Page 77: Spectrum Display
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Spectrum Display Click the Display button on the User Interface Control Bar to switch to Spectrum Display . The top plot shows the voltage from the photo-detector as the laser scans across the three main isotopomers of H O absorption features (near 1.38 microns).
Page 78: File Transfer Menu
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 File Transfer Menu Use the File Transfer menu to access data collected by the analyzer. Each time the analyzer is re-started, three files are created (f, l and s) with the most •...
Page 79: File Type Details
Long Format Data File: lwiaDDMMYYYY_lxxxx.txt This file is for diagnostic purposes and may be requested by ABB-LGR to diagnose an issue. Spectra Data File: lwiaDDMMYYYY_sxxxx.txt This file is for diagnostic purposes and may be requested by ABB-LGR to diagnose an issue.
Page 80 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 describes the standard data file columns. Table 11 Table 11: Standard Data File Column Names and Descriptions Column Name Description Timestamp of recorded injection Time Injection number Injection No. Vial Name Sample name Vial S/N Sample serial number...
Page 81: Transfer Data Files
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Transfer Data Files To transfer data files from the analyzer hard drive to a USB storage device: Click the Files button on the User Interface Control Bar (Figure 63) to access the File Transfer Menu .
Page 82 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 When you have finished transferring files: 5. Click the Unmount USB button. Wait for the Safe to Remove USB Memory Device message before removing the USB memory device. 6. Click Close to exit the File Transfer Menu . Removing the USB memory device before seeing the Safe to Remove pop- up message may result in loss of data.
Page 83: Types Of Directories In The Local Hard Drive
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Types of directories in the local hard drive The analyzer hard drive contains two types of directories: Daily Directory • Archive Directory • Daily Directory The local hard drive (Figure 72) creates a daily folder containing new data files for each day that the analyzer operates.
Page 84 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Archive Directory The local hard drive (Figure 72) creates an archived folder containing zipped files organized by date. (Figure 74) To access the archived files, double-click the Archive folder. (Figure 72) Each file is a single zipped .txt file, using the following convention: YYYY-MM-DD.zip.
Page 85: File Transfer Error Screen
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 File Transfer Error Screen The File Transfer Error screen (Figure 75) displays when: The USB Key does not have enough storage space. • The device is not recognized. • Try again with a correctly inserted USB device. Figure 75: File Transfer Error...
Page 86: Setup Menu
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Setup Menu The Setup menu allows access to additional configurations and services. To enter Setup mode: Click the Setup button on the User Interface Control Bar . (Figure 76) Figure 76: Setup button on the User Interface Control Bar 2.
Page 87: Time/Files Tab
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 The Setup menu has function tabs at the top of the screen that allows you to configure the analyzer mode and settings. (Figure 77) These tabs let you: Manage file saving settings •...
Page 88 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Local Time Zone The Local Time Zone menu lets you adjust the current local time zone by selecting an option from the drop-down selection box. Clock The Clock menu lets you manually adjust the current time and date settings. File Output The File Output menu lets you adjust the timestamp format of the data files.
Page 89 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 LWIA/LIMS State LIMS (Library Information Management System) users can designate a unique ID for the Liquid Water Isotopic Analyzer. This ID can be changed upon receipt of the analyzer if the current ID is already in use in their laboratory.
Page 90 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 To change the Instrument ID: Click the Instrument ID drop-down box to select an analyzer specific ID. (Figure a. Click on the designated letter. The options within the box are alphabetical: A-Z.
Page 91: Autoinjector
Use the tab to make adjustments to the autoinjector settings. For optimal performance, ABB-LGR recommends the default settings shown below. (Figure 81) Figure 81: Autoinjector Settings Menu The options within the autoinjector tab are as follows: Prep Fill Settings •...
Page 92 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 The following tables provide a description of the options within the autoinjector tab. Prep Fill Settings Table 13: Prep Fill Settings Dialog Item Function Sample Volume The injected volume for samples and standards (nL) Fill Speed (nL/s) The rate the syringe is filled...
Page 93 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Measure Fill Settings Table 15: Measure Fill Settings Dialog Item Function Sample Volume (nL) The injected volume for samples and standards Fill Speed (nL/s) The rate the syringe is filled Eject Speed (nL/s) The rate the syringe is withdrawn from the vial Pull up Delay (ms)
Page 94 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Syringe Idle Position Table 18: Syringe Idle Position Dialog Item Function Syringe idle enable This checkbox enables/disables the syringe idle feature. When enabled, at the end of a run, the syringe will move to a designated vial and hold 1.2uL of water to prevent the syringe from drying out.
Page 95: Laser Adjust Tab
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Laser Adjust Tab Use the Laser Adjust tab to manually adjust the laser’s wavelength to compensate for any cumulative drift. (Figure 82) Laser adjustment may be needed for the following reasons: The laser’s wavelength has drifted beyond the target range of the analyzer.
Page 96 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Manually Adjust the Laser Offset Click the Setup button on the User Interface Control Bar . (Figure 76) 2. Select the Laser Adjust tab at the top of the screen. (Figure 82) 3.
Page 97: Septum Tab
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Septum Tab The Septum tab (Figure 84) includes a step-by-step process to change the septum. The septum should be changed after ≈ 750-1000 injections. The screen has five fields showing the progress of each step. Follow the sequence correctly to prevent contamination of the cavity.
Page 98: Shutting Down The Analyzer
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Shutting Down the Analyzer To shut down the analyzer: Click the Exit button on the User Interface Control Bar . (Figure 85) Figure 85: User Interface Control Bar Exit Button 2.
Page 99 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 5. When the “You may turn off the instrument” message displays (Figure 88), shut off power to the analyzer by pushing the OFF switch on the front of the analyzer. (Figure 2) Figure 88: Final Shutdown Screen Failure to wait for the power down command to display before shutting...
Page 100: Example Run Configurations
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Example Run Configurations This section describes several example run configurations to use as starting points to define more complex measurement sequences. These configurations are pre-installed on the analyzer. The configurations are organized based on the samples’ expected δD range: Standard Natural Range: -150 ‰...
Page 101 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 4. Click on the Examples folder. (Figure 90) Figure 90: Click on the Examples Folder 5. Within the Examples folder are 3 additional folders containing examples of: (Figure a. Enriched example run configurations (Enriched folder) b.
Page 102 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 6. Click one of the 3 folders to access the files. Files of type: (*.vials) d. The Enriched folder contains these run configurations: i. EnrichedHPExample.vials (high precision) ii. EnrichedHPSplineExample.vials (high precision optimized for spline) iii.
Page 103 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 8. The Configuration Display will be populated with the selected run configuration. Figure 93 Figure 93: Configuration Display with an example run configuration 9. Click on Make Run. (Figure 68) 10.
Page 104: Injection Pattern Conventions
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Injection Pattern Conventions An interleaved run is defined as having standard and sample injection sets measured in alternating order. Figure 94 shows an example of an interleaved data set with rotating standards in between samples.
Page 105: Ignored Injections
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Ignored Injections: Ignored injections are the initial injections which will be excluded in the Post Analysis Software each time a standard or sample is changed to mitigate analyzer memory effects, regardless of any other issue with those injections.
Page 106: Spline Fitting Run Configuration
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Spline Fitting Run Configuration When using any of the spline calibration functions in the Post Analysis Software, it is recommended to include in the LWIA run configurations additional standard measurements at the beginning and end of the run. This setup allows the benefits of the spline calibration to extend to all samples in the run.
Page 107: Injection Patterns For Standard Natural Range, Full Natural Range, And Enriched Samples
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Injection Patterns for Standard Natural Range, Full Natural Range, and Enriched Samples A measurement is defined as 6 injections of the same sample. High performance is defined as the average of 10 measurements of the same sample. The length of time for a run is dependent on the user configuration and how often standards are used for referencing.
Page 108 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Standard Natural Range Standard Natural Range High Precision Injection Patterns Figure 96: Standard Natural Range High Precision Injection Patterns...
Page 109 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Standard Natural Range High Throughput Injection Patterns Figure 97: Standard Natural Range High Throughput Injection Patterns...
Page 110 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Full Natural Range Choose the appropriate standards for the full natural range. Example: Standard 1: VSMOW2 • Standard 2: GISP • Standard 3: SLAP2 • Full Natural Range High Precision Injection Patterns Figure 98: Full Natural Range High Precision Injection Patterns...
Page 111 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Full Natural Range High Throughput Injection Patterns Figure 99: Full Natural Range High Throughput Injection Patterns...
Page 112 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Enriched Choose the appropriate standards for the enriched range. Example: Standard 1: LGR5C • Standard 2: ER2 • Standard 3: ER4 • High Precision Injection Pattern Figure 100: Enriched High Precision Injection Patterns...
Page 113 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 High Throughput Injection Patterns Figure 101: Enriched High Throughput Injection Patterns...
Page 114: Data Analysis
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Data Analysis Use the provided Post Analysis Software for data analysis. (Figure 102) The software calculates adjustments to the measured values of δD and δ O based on the differences between known and measured values of water isotope standards, and it reports a processed value.
Page 115: Maintenance
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Maintenance Daily Operation Checklist Table 19 describes routine maintenance tasks that keep your analyzer operating smoothly. Table 19: Maintenance Checklist Frequency Task Every 1-2 days Replace the septum as described on page 122. •...
Page 116: Mirror Ring-Down Time And Maintenance
Periodically note the ring-down time. If a significant reduction in ring-down time occurs: Request a mirror cleaning kit from ABB-LGR. • If further maintenance is required, contact ABB-LGR for service. • Technical Support: icos.support@ca.abb.com Only authorized persons may open the analyzer cover or perform internal maintenance.
Page 117: Routine Maintenance Of The Septum, Sample Transfer Line And Injector Block
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Routine Maintenance of the Septum, Sample Transfer Line and Injector Block ALWAYS vent the cavity when cleaning the sample transfer line or injector block. Failure to do this may cause contamination of the cavity. Vent the Analyzer Before Routine Maintenance Before the septum can be changed or the transfer tube and injector block can be cleaned, the analyzer cavity must be vented.
Page 118 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 5. When the Venting Complete step reaches 100%, the screen will display Change Septum, Click NEXT when done. Figure 104) Begin the cleaning process/septum change process. Refer to these sections for cleaning instructions: Clean the Sample Transfer Line and the Attached Filter Assembly (Refer to Page 119) Remove Septa Bits from the Injector Block (Refer to page 121)
Page 119: Clean The Sample Transfer Line And The Attached Filter Assembly
Assembly Small pieces of septa accumulate over time in the sample transfer line and injector block. ABB-LGR recommends that the sample transfer line and filter assembly be cleaned each time the septum is changed. Before the sample transfer tubing can be cleaned, the analyzer cavity must be vented.
Page 120 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 2. Cap the port on the analyzer immediately, using the provided ¼” Swagelok cap and finger-tighten firmly. This step is critical in preventing dust from entering the analyzer. 3. Remove transfer tube from heater block using 9/16” wrench at the ¼” Swagelok connector.
Page 121: Remove Septa Bits From The Injector Block
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Remove Septa Bits from the Injector Block Before cleaning the injector block, the analyzer cavity must be vented. Refer to Vent the Analyzer Before Routine Maintenance on page 117 before proceeding with this procedure.
Page 122: Replace The Septum
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Replace the Septum Before the septum can be replaced, the analyzer cavity must be vented. Refer to Vent the Analyzer Before Routine Maintenance on page 117 before proceeding with this procedure.
Page 123 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 For septa that are either partially or not pre-drilled: Verify that the black septum support is positioned inside the injector block. (Figure 108) 2. Slide the septum nut onto the provided blunt 22-gauge needle ( Hamilton AS top style, or similar).
Page 124 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 4. Without puncturing the septum with the needle, place the septum nut with needle onto the injector block and hand-tighten the septum nut firmly, using the provided heat-resistant gloves when hot. (Figure 112) Figure 112: Tighten Septum Nut Failure to adequately hand-tighten the septum nut may cause air leakage and contamination during pump out.
Page 125 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 5. Manually actuate the blunt 22-gauge needle five times to confirm that the septum is adequately pre-drilled. (Figure 113) Figure 113: Actuate Needle 6. Remove the needle fixture from the septum nut. If the septum continuously shreds/deteriorates: Check the position of the septum injection port.
Page 126: Clean The Injector Block
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Clean the Injector Block Salt deposits and small pieces of septa accumulate over time in the injector block. After approximately 1– 2 months of continuous use (15000 – 20000 injections), clean the injector block to remove deposits.
Page 127 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 To clean the injector block Soak the injector block in an ultrasonic bath of deliming solution (35% phosphoric acid) diluted 1:1 for 1 hour (Nyco brand Low Foam Delimer, Product # NL352, recommended).
Page 128 Failure to do so may cause air leakage and contamination to the cavity. Refer to Initiate Cavity Pump Out on page 129 to continue. ABB-LGR recommends using an additional injection block to minimize down time from cleaning.
Page 129: Initiate Cavity Pump Out
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Initiate Cavity Pump Out When the routine maintenance is complete, click NEXT to initiate the cavity pump out. (Figure 114) Figure 114: Septum Install Complete The filter assembly, sample line, and injector block MUST be attached before clicking NEXT.
Page 130 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 2. The cavity will pump out for one minute. (Figure 115) Figure 115: Analyzer pumps out cavity The indicator box displays Septum Ready when the process is complete . (Figure 116) Figure 116: Septum Ready 4.
Page 131: Caring For Syringes
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Caring for Syringes Examine the syringes before each run on the Liquid Water Isotopic Analyzer. Salts and septum bits can deposit inside the syringe over time and impede its’ performance. Regular inspection and cleaning of the syringe will extend syringe lifetime and performance.
Page 132: Maintain The Drierite® Laboratory Drying Unit
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Maintain the Drierite® Laboratory Drying Unit Dry air is used to flush the measurement cell. The lifetime of the provided Drierite® laboratory drying unit is determined by ambient humidity levels; therefore, the desiccant may need to be regenerated or replaced more frequently in humid areas.
Page 133 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 3. If the blue region is less than 1”, regenerate or replace the desiccant. a. Remove the top of the column, using the provided wrenches. Carefully set aside the lid, metal spring, metal plate, and felt filter. b.
Page 134: Replace The Fuse
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Replace the Fuse If the fuse blows or is otherwise damaged, the analyzer automatically turns off. To replace the fuse: Flip the Power switch to OFF on the left side of the analyzer. (Figure 2) 2.
Page 135 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 5. The fuse is located on the upper portion of the analyzer on the left side above the internal pump. (Figure 120) Figure 120: Analyzer Fused Inlet 6. Pull the fuse out of the fuse fixture. (Figure 121) Figure 121: Remove the Fuse...
Page 136 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 7. Push the new fuse into the fuse fixture. (Figure 122) Figure 122: Install the New Fuse in the Fuse Holder 8. Carefully close the case top and push the latches to lock the case. 9.
Page 137: Troubleshooting Guide
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Troubleshooting Guide The Liquid Water Isotopic Analyzer should provide accurate isotope ratios. High throughput mode: • H to within ± 0.6‰ (600 per meg) δ O to within ± 0.2 ‰ (200 per meg) δ...
Page 138 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Table 20: Troubleshooting Injection Performance Flags Flag Description and Suggested Solutions norm The analyzer is operating within normal parameters. No action is required. prep A preparatory injection is in progress. No action is required. The temperature of the water vapor is changing too rapidly during a single temp measurement.
Page 139: Confirmation Of Measured Spectrum
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Confirmation of Measured Spectrum The Spectrum Display should look similar to the spectra shown in Figure 123. Figure 123: Confirmation of Spectrum Display, Laser A After an injection, the Transmitted Intensity (top plot) should show a maximum transmission signal of 0.3 –...
Page 140 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Contact ABB-LGR section on page 144 before contacting ABB-LGR for further • assistance. High number of Pres flags: Over time, small bits of septa can accumulate in the • block, resulting in incomplete evaporation of the water before the measurement begins.
Page 141: Confirmation Of Injected Volume
Clean the Injector Block on page 126. If the injected volume continues to fluctuate or exceed bounds, follow steps • described in Contact ABB-LGR on page 144 before contacting ABB-LGR for further assistance. Confirmation of Measured Isotope Ratios...
Page 142: How To Perform An Lgr Diagnostic Run
The ABB-LGR customer service department uses the LGR Diagnostic run to troubleshoot performance issues with your analyzer. Running this test before contacting ABB-LGR will help you to receive a faster diagnosis. ABB-LGR will use the Standard Data File and Long Format Data File for troubleshooting: Standard Data File: lwiaDDMMYYYY_fxxxx.txt •...
Page 143 8. Toggle the Display button on the User Interface Control Bar (Figure 63) to switch to the Run Display . (Figure 69) 9. Click Start. The test is ≈ 12 hours long. 10. When the test is complete, send the Standard Data File (F file) and the Long Format Data File (L file) to ABB-LGR.
Page 144: Contact Abb-Lgr
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Contact ABB-LGR For faster service, before contacting ABB-LGR to help troubleshoot an issue with the analyzer, prepare the following information to send to the customer service department: Record the analyzer’s serial number (located within the inside of the analyzer) •...
Page 145: Appendix A: Injected Volume Correction
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Appendix A: Injected Volume Correction Measured delta values can have a dependence on the water concentration in the analyzer. When a user measures this dependence, the Post Analysis Software can correct the measured delta values for small variations in the water concentration due to syringe instability.
Page 146 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 d. A pop-up window will appear. (Figure 127) Click on the ULWIA_Configuration_Files folder. Figure 127: InjVolCorr.lims.csv File e. Single click on the correct InjVolCorr.lims_LGR3.csv file that corresponds with your water standard and press Open to load the injected volume configuration.
Page 147 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 g. Click Display on the User Interface Control Bar (Figure 126) to cycle to the Run Display. h. The Run Display will be populated with the Injected Volume Correction run configuration. (Figure 129) Press Start.
Page 148 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 4. Plot the graphs: a. Measured isotope ratios (not delta values) of the isotopic reference water vs. normalized injected volumes i. Repeat for each isotope. The column name in the data file will vary depending on the version of your analyzer.
Page 149: Appendix B: About Gas Analyzers And Laser Absorption Spectroscopy
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Appendix B: About Gas Analyzers and Laser Absorption Spectroscopy Conventional Laser Absorption Spectroscopy For gas measurements based on conventional laser-absorption spectroscopy (Figure 131), a laser beam is directed through a sample, and the mixing ratio (or mole fraction) of a gas is determined from the measured absorption using Beer’s Law, which may be expressed: Where: is the transmitted intensity through the sample at frequency...
Page 150 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Integration of the measured spectra with the measured values of: Gas temperature • Gas pressure • Path length • Line strength of the probed transition • Enables you to determine the mole fraction directly from the relation: Use this equation to determine gas concentrations, even in hostile environments without using calibration gases or reference standards.
Page 151: Abb-Lgr's Off-Axis Integrated-Cavity Output Spectroscopy (Off-Axis Icos)
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 ABB-LGR’s Off-Axis Integrated-Cavity Output Spectroscopy (Off-Axis ICOS) Off-Axis ICOS uses a high-finesse optical cavity as an absorption cell as shown in Figure 132. Unlike multi-pass detectors, which are typically limited to path lengths of less than two hundred meters, an Off-Axis ICOS absorption cell effectively traps the laser photon so that, on average, they make thousands of passes before leaving the cell.
Page 152: Appendix C: Accessing Data Using The Ethernet
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Appendix C: Accessing Data Using the Ethernet Appendix B explains how to access the analyzer data directory as a Windows Share using an Ethernet connection on a local area network (LAN). The data files stored on the internal hard disk drive of the analyzer can be accessed as a Windows Share over a Local Area Network (LAN) Ethernet connection.
Page 153: Additional Notes
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Additional Notes The analyzer shared data directory is in the LGR workgroup. If it is not visible, browse for it in the Windows Network Neighborhood by entering the IP address of the analyzer. Figure 78 shows the location of the IP address.
Page 154: Appendix D: Wireless Router Setup For Liquid Water / Pal3 Autoinjector Compatibility
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Appendix D: Wireless Router Setup for Liquid Water / PAL3 Autoinjector Compatibility The PAL3 autoinjector includes a GL-MT300N-V2 Mini Smart Router for use when the Local Area Network (LAN) is not available. When Wi-Fi is ON, the analyzer and PAL3 autoinjector will obtain a Dynamic Host Configuration Protocol (DHCP) address from the router.
Page 155 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 The Dynamic Host Configuration Protocol (DHCP) is enabled on the PAL3 autoinjector to automatically assign an IP address. 4. Reboot the analyzer and autoinjector. 5. The analyzer IP address will be in the format 192.168.8.XXX, and will be displayed on the Time/Files screen.
Page 156 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 7. Click on the Autoinjector tab at the top of the screen to access the Autoinjector settings menu. (Figure 137) Figure 137: Autoinjector screen 8. Click on the Find auto injector(s) tab. (Figure 137) 9.
Page 157: Connect To A Windows Computer
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Connect to a Windows Computer On your personal Windows computer: Click on the network icon at the bottom right corner of the screen. (Figure 139) Figure 139: Wireless connection 2. From the list of wireless networks on the Windows Wireless Networks dialog-box (Figure 140) select the router.
Page 158: Wireless Control Using Remote Device
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 5. Type the password listed on the router into the Security key box: The password is goodlife. (Figure 142) Figure 142: Security key box 6. Press OK. (Figure 142) Wireless Control Using Remote Device For wireless control of the analyzer using a remote device, install the appropriate Virtual Network Client (VNC) software on your remote device.
Page 159: Reconfiguring The Wireless Router
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Reconfiguring the Wireless Router If the router is to be used with a different analyzer, a new IP address will need to be assigned. To setup the wireless router: When the analyzer software is active, hover the mouse in the top, left corner of the screen and click on the icon.
Page 160 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 3. Mozilla Firefox will open. Type 192.168.8.1. Press ENTER. (Figure 144) Figure 144: Mozilla Firefox screen 4. Type the password into the box: Password: 123456789 (Figure 145) Figure 145: Enter password...
Page 161 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 5. Click Advanced Settings in the top right corner. (Figure 146) Figure 146: Advanced settings 6. Re-enter the password, and click Reset. Password: 123456789 (Figure 147) Figure 147: Enter password 7.
Page 162 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 8. In the dropdown menu, select DHCP and DNS. (Figure 149) Figure 149: DHCP and DNS 9. Scroll down to Static Leases to set up the MAC-Address and IPv4 address for the analyzer.
Page 163 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 The terminal window will display the MAC address of the analyzer. (Figure 152) Figure 152: MAC address d. Return to the Static Leases section in the Web Brower (Figure 153). Click on the Mac-Address drop-down selection box and choose the same address that is listed in the Terminal Window.
Page 164 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 10. Select the Network tab at the top of the web browser. (Figure 154) a. Scroll to Firewall and press ENTER. Figure 154: Network tab 11. Set Port Forwards for VNC, SSH, MODBUS, and SMB: a.
Page 165 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 b. Scroll to New port forward and set VNC: (Figure 156) Type Name: VNC Set External port to 5900 iii. Set Internal port to 5900 Select the Internal IP address drop down box and select the analyzer’s IP address.
Page 166 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 e. Set SMB: (Figure 159) xi. Type Name: SSH xii. Set External port to 445 xiii. Set Internal port to 445 xiv. Select the Internal IP address drop down box and select the analyzer’s IP address.
Page 167 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 12. Re-type 192.168.8.1 and press ENTER to return to the Main Window . (Figure 161) Figure 161: Main Window 13. The SSID written on the router should match the SSID within Settings . (Figure 161) 14.
Page 168: Appendix E: Set Up Devices For Remote Access Using Vnc Software
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Appendix E: Set Up Devices for Remote Access Using VNC Software Listed below are three types of devices that can be connected to the analyzer through the wireless router to access information: Android OS based devices (smart phones and tablets) •...
Page 169 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Complete instructions for installing the Android-vnc-viewer can be found online http://code.google.com/p/android-vnc-viewer/wiki/Documentation Figure 162: Android-vnc-viewer Install Screen 9. Open the VNC application on the Android device by selecting the VNC application icon.
Page 170 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 11. In the Address field, enter the address of the analyzer (192.168.100.100 or 192.168.100.101). The IP address of the analyzer will be whichever address the Android device is not. For example, if the IP address of the Android device that was displayed in Step 7 is 192.168.100.101, then the IP address of the analyzer will be 192.168.100.100.
Page 171 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Set up VNC Software on iOS Devices On the iOS device, go to Settings > WiFi, then select the network from the list. 2. Connect to the wireless SSID network listed on the router sticker. 3.
Page 172 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 9. Install the VNC software by searching and installing it from the App store. a. Mocha VNC Lite for iOS is the software used in this example. (Figure 168) b. An Internet connection is required for this step. Complete instructions for installing Mocha VNC Lite for iOS can be found online at: http://www.mochasoft.dk/iphone_vnc_help2/help.htm.
Page 173 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 12. Enter the analyzer’s address in the VNC server address field (192.168.100.100 or 192.168.100.101). The IP address of the analyzer will be whichever address the iOS device is not. For example, if the IP address of the iOS device that was displayed in Step 8 is 192.168.100.101, then the IP address of the analyzer will be 192.168.100.100.
Page 174 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 15. To connect the iOS device to the analyzer, tap the IP Config you set up. The analyzer software will display on the device. (Figure 172) The screen size is adjustable to fit the screen of the device.
Page 175 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Set up VNC Software on Windows Devices On the Windows device, open Wireless Router options. 2. Locate the sticker on the router. (Figure 142) 3. Click on the Wireless Network Connections icon in the bottom left of the screen (Figure 173) to open the Windows Wireless Networks dialog-box.
Page 176 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 7. Type the password listed on the router into the Security key box: The password is goodlife. (Figure 142) Figure 176: Security key box 8. Press OK. (Figure 142) 9. Install the VNC software by going to the RealVNC website and downloading the RealVNC Viewer “EXE”...
Page 177: Appendix F: Mirror Cleaning Procedure
• Mirror cleaning kits are included as part of the (recommended) Maintenance kits (MTN-CLEAN-M). See Optional Components. If further maintenance is required, contact ABB-LGR for service. • Technical Support: icos.support@ca.abb.com Only authorized persons may open the analyzer cover or perform internal maintenance.
Page 178 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Figure 178 shows the mirror cleaning kit. Figure 178: Mirror Cleaning Kit...
Page 179 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Remove the Mirrors Place the analyzer on a flat, stable surface in a clean area. Perform this procedure in a clean, non-carpeted room. Airborne particles (particularly carpet fibers) are attracted to freshly cleaned mirrors and can re-contaminate the mirror before it can be replaced onto the cavity.
Page 180 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 5. Open the case top. Locate the cavity as shown in Figure 180. Figure 180: Ultra-Portable Case Open 6. Remove the safety shield by un-screwing the two flat-head screws and carefully lifting the shield off of the laser assembly.
Page 181 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 7. Loosen the detector assembly by un-screwing and removing the Philips head screws on either side of the bracket. (Figure 182) Figure 182: Remove the screws from the Detector Bracket 8.
Page 182 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 10. The flange at the front of the optical fiber launch tray has a wire connection. Unplug the wires by separating the connectors as shown in Figure 184. The detector flange does not have this connector. Figure 184: Separate Connectors 11.
Page 183 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 12. Remove any dust or particles from the inside of the measurement cell by releasing a puff of compressed gas through the measurement cell from a distance of six to eight inches.
Page 184 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 15. Place the mirror flange on top of the Teflon tube (provided) and carefully push the flange downwards until the mirror pops out of the flange O-ring. (Figure 188) Figure 188: Releasing the Mirror...
Page 185 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Clean the Mirrors This section explains how to clean the mirrors, using a technique called drag wiping . Place the mirror on the mirror cleaning block with the arrow on the mirror edge pointing down (anti-reflection side up) as shown in Figure 189.
Page 186 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 3. Transfer a very small amount of fresh acetone from the Acetone Screw-Cap Bottle to the Acetone Dropper Bottle . Use just enough to rinse the dropper bottle. 4. With the dropper top closed, shake the dropper bottle to rinse the interior of the bottle and discard the rinse acetone.
Page 187 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 8. Using the acetone dropper bottle , place 4 drops of Acetone onto the tissue at the center of the mirror. The solvent will wick to the edge of the mirror. (Figure 192) Figure 192: Drag Wipe Step 1 9.
Page 188 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 10. Discard the used tissue. 11. Lay a clean tissue on top of the mirror 12. Place 4 drops of Methanol on a clean tissue and repeat the drag wipe process. 13.
Page 189 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Reassemble the Analyzer Place the mirror back into the flange with the HR side down (arrow down), so that the HR side will be facing towards the measurement cell. When placed into the flange, the mirror sits on the outer O-ring.
Page 190: Reorder Information
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Reorder Information 30ml LDPE Screw Top Bottle (Nalgene Part #: 2003-0001) • 30ml LDPE Dropper Bottle (Nalgene Part #: 2411-0030) • Acetone HPLC Plus, ≥ 99.9% (Sigma-Aldrich Part #: 650501) •...
Page 191: Appendix G: Ultra-Portable Accessories
This section lists the optional items that are available for Ultra-Portable analyzers. Verify that you have received your accessories before you begin installation. For price and please availability, contact icos.support@ca.abb.com and your local sales representative. Optional accessories include: Maintenance Kit: MTN-ULWIA •...
Page 192 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Ultra-Portable Back Pack: ACC-UP-BP • Figure 198: Ultra-Portable Field Backpack Battery Hook-up Kit ACC-UP-12V • Figure 199: Battery Hook-Up Kit for Lead - Acid Batteries Replacement Fuses, In-line Filters, Side Covers: 915-9300-0000 •...
Page 193: Appendix H: The Pal3 Lsi Autoinjector
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Appendix H: The PAL3 LSI Autoinjector The PAL3 LSI User Manual is provided with the autoinjector and provides diagrams, configuration data, and function control data to assist you when using the autoinjector with the analyzer.
Page 194: Function Keys
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Function Keys The button functions are as follows: Menu Button A (Options) : Options for a particular menu are assigned to the • corresponding function key on the left side. Menu Button B (Default Action) : Reserved for default actions, such as Save, •...
Page 195: Set Regional Settings
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Set Regional Settings To set the regional settings: Press A and B Menu Buttons simultaneously to change Access Level 2. Scroll to Extended User Level 3. Press Enter Note: Steps 1-3 will allow the user to enter Service mode. 4.
Page 196 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 7. Scroll to Installation . (Figure 206) Figure 206: Installation 8. Press Enter. 9. Scroll to Set Regional Settings . (Figure 207) Figure 207: Scroll to Set Regional Settings 10. Press Enter.
Page 197 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 11. Press Language and scroll to the language of your choice (example: English ). (Figure 208) Figure 208: Select Language 12. Press Enter. 13. Press Region and choose a region of your choice (example: English (United States) ). (Figure 208) 14.
Page 198: Set Date And Time
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Set Date and Time To set the date and time: Press A and B Menu Buttons simultaneously to change Access Level 2. Scroll to Extended User Level 3. Press Enter a.
Page 199 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 7. Scroll to Installation . (Figure 211) Figure 211: Installation 8. Press Enter. 9. Scroll to Set Date and Time . (Figure 212) Figure 212: Set Date & Time 10. Press Enter.
Page 200: Locate Ip Address
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 11. Scroll to Time Zone . (Figure 213) Figure 213: Time Zone 12. Press Enter. 13. Scroll to -8:00 Pacific Time (US & Canada). (Figure 213) 14. Press Enter 15. Press OK (Button B) Locate IP address To locate the IP address of the PAL3 autoinjector: Press Options (Button A)
Page 201: Enter A Unique Static Ip Address
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Enter a Unique Static IP Address The Dynamic Host Configuration Protocol (DHCP) is enabled on the PAL3 autoinjector to automatically assign an IP address to the autoinjector. A unique static IP address can be set by de-selecting DHCP and manually entering an IP address.
Page 202 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 5. Scroll to Service . (Figure 215) Figure 215: Scroll to Service 6. Press Enter. 7. Scroll to Installation . (Figure 216) Figure 216: Installation 8. Press Enter. 9. Scroll to Setup Network . (Figure 217)
Page 203 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Figure 217: Setup Network 10. Press Enter. 11. Scroll to DHCP . (Figure 218) Figure 218: Set the IP Address...
Page 204 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 12. Press Enter to uncheck the box. When the DHCP box is checked, an IP address will be automatically assigned to the PAL3 autoinjector. 13. Scroll to IP Address . (Figure 218) 14.
Page 205: Firmware Version
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Firmware Version On the Home screen, press Options (Button A). (Figure 220) Figure 220: Options 2. Scroll to About and press Enter. (Figure 221) Figure 221: About 3. Scroll to Product Version to see the latest version. (example: 2.4.1)
Page 206: Adjust Location Settings
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Adjust Location Settings The autoinjector firmware is pre-installed. Do NOT change the parameters unless the autoinjector is not functioning properly. This section details each step: Teach vial positions • Teach the position of the injection block •...
Page 207 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Teach Vial Positions If other trays and vials are used besides the ones provided, the tray positions may need to be re-taught. Place 3 trays onto the tray holder. (Figure 222) 20.
Page 208 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 3. Enter the sequence of commands using the keypad dial wheel and center button as shown in Figure 203. a. Press A and B Menu Buttons simultaneously to change Access Level b.
Page 209 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 g. Select Rack 1 . (Figure 226) Figure 226: Rack 1 h. Press Options (Button A) Scroll to Teach PALmodule. This will define the x, y and z-axes. (Figure 227) Figure 227: Teach PALmodule...
Page 210 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Manually move the vertical arm to vial #1 in tray #1. (Figure 228) k. Press Save (Button B) Figure 228: Move vertical arm to vial #1 Press Enter to fine tune adjustment of x, y or z position. (Figure 229) Figure 229: Fine Tune Adjustment The needle guide must be centered over the vial in position 1.
Page 211 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 m. Press Next (Button B) n. Manually move the vertical arm to vial #46 in tray #2. (Figure 230) Figure 230: 2nd Teach Point in Front Corner Left o. Press Save (Button B) Figure 231: Move vertical arm to vial #46...
Page 212 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 p. Press Enter to fine tune adjustment of x, y or z position. (Figure 232) Figure 232: Fine tune adjustment q. Press Next (Button B) Manually move the vertical arm to vial #54 in tray #1. (Figure 233) s.
Page 213 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Press Enter to fine tune adjustments of x, y or z positions. (Figure 234) Figure 234: Fine tune adjustment u. Press Next (Button B) Press OK to move the PALhead back to Home position (Button B). (Figure 235) Figure 235: Move PALhead back to Home w.
Page 214 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Teach the Position of the Heated Injection Port Unplug the power cord to the injector block and allow the block to cool to prevent injury. Remove the protective blue cap from the heated injector block. (Figure 236) Figure 236: Remove the cap from the injector block Place the alignment tool on top of the septum cap.
Page 215 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 4. Enter the sequence of commands using the keypad dial wheel and center button as shown in Figure 203. 5. Press A and B Menu Buttons simultaneously to change Access Level 6.
Page 216 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 13. Manually move the vertical arm to the heated injector block so that the conical syringe guide sits securely within the alignment tool. (Figure 240) Figure 240: Syringe Guide 14. Press Save (Button B) Figure 241: Move the head to the teach point...
Page 217 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 15. Press Enter to fine tune adjustment of x, y or z positions. Verify that the arm position is directly over the injector port. Figure 242: Fine tune adjustment 16. Press Next (Button B) 17.
Page 218: Changing To A Different Type Of Syringe
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Changing to a different type of syringe ABB-Los Gatos Research recommends using the provided 1.2uL Hamilton syringes. However, syringe types can be changed by following these steps: Enter the sequence of commands using the keypad dial wheel and center button as shown in Figure 203.
Page 219: Backup
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Backup To create a backup: Insert the USB into the port on the back panel of the autoinjector. A USB icon will appear on the screen. (Figure 244) Figure 244: USB icon 2.
Page 220 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 4. Scroll to Create Configuration Backup and press Enter. (Figure 246) Figure 246: Configuration Backup 5. Verify the Serial Number on the screen is the same as what is printed on the right side of the autoinjector arm.
Page 221 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 6. Press Start (Button B). The configuration backup will begin. The screen displays the percentage of completion. (Figure 248) Figure 248: Completion % 7. When complete, the screen will show the message “ The configuration backup has been created successfully”...
Page 222: Shutdown Process
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Shutdown Process To shutdown the autoinjector: 1. Press Options (Button A) 2. Scroll to Shutdown and press Enter. (Figure 250) Figure 250: Shutdown 3. The “ Are you sure you want to shut down ?” prompt will appear on the screen. (Figure 251) 4.
Page 223 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 5. When the “ Shutdown Completed! ” message appears on the screen, switch the power button to OFF. (Figure 252) Figure 252: Shutdown completed!
Page 224: Error Messages
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Error Messages Pending Message (Blinking Envelope) Pending Messages typically derive from a collision during the homing process. Check the homing path of all axes and reboot the system again. If there is a blinking envelope at the top of the screen: Press Options (Button A) 2.
Page 225 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 X Drive 1 is not homed. This message appears on the PAL3 display screen. It may be due to an improper shutdown. See page 222 for details on how to properly shut down/reboot the analyzer. To re-home the X position: Scroll to RobotArmLeft 2.
Page 226 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Error Penetration Depth If the Error Execute [PenetrateObject] ActivityError!Runtime Error ‘General_Movement Timeout Occu rred’ message appears on the analyzer Run Screen, the penetration speed is set too low. The PAL3 autoinjector times out if it takes too long to reach the penetration depth.
Page 227 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 2. Select the Autoinjector tab at the top of the screen. (Figure 257) Figure 257: Autoinjector tab Increase the Penetration speed in the Prep Fill Settings pane and the Measure Fill Settings pane.
Page 228: Appendix I: Cables
USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Appendix I: Cables Table 21 describes the power cables shipped with your analyzer. Table 21: Power Cables Region Cable Specifications Australia and New Zealand United Kingdom...
Page 229 USER MANUAL | ICOS | INSTRUCTIONS | UM/ICOS-EN REV. B.2 Europe United States...
Page 230 We reserve the right to make technical changes or modify the contents of this document without prior notice. With regard to purchase orders, the agreed particulars shall prevail. ABB does not accept any responsibility whatsoever for potential errors or possible lack of information in this document.

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