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Siemens ADVIA 2120 Operator's Manual

Hematology systems

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ADVIA

2120 / 2120i Hematology Systems

®

Operator's Guide

10708774 Rev. 04, 2021-03

ADVIA

2120

®

ADVIA

2120i

®

H e m a t o l o g y S y s t e m s

Table of Contents

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Table of Contents

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Summary of Contents for Siemens ADVIA 2120

Page 1 ADVIA 2120 ® ADVIA 2120i ® H e m a t o l o g y S y s t e m s ADVIA 2120 / 2120i Hematology Systems ® Operator’s Guide 10708774 Rev. 04, 2021-03...
Page 2 If the system is used in a manner differently than specified by Siemens Healthineers, the protection provided by the equipment may be impaired. See warning and hazard statements. 10708774 Rev. 04...
Page 3 How the ADVIA 2120/2120i Hematology System Works . . .48 How the ADVIA 2120/2120i Software Works ... .49 Status lines......... . . 50 Menus .
Page 4 Turning the system off: Routinely ......62 Exiting the ADVIA 2120/2120i Software ....62 Working with the Software Working with the ADVIA 2120/2120i Shell.
Page 5 ADVIA 2120/2120i Operator’s Guide Analyzer Status Tab ........100 Analyzer Status Screen .
Page 6 ADVIA 2120/2120i Operator’s Guide Creating Workorders ........337 Listing the Pending Workorders.
Page 7 ADVIA 2120/2120i Operator’s Guide Adjusting the Pneumatic Regulators ..... . 383 Replacing the Perox Lamp ......384 Maximizing the Perox Lamp Output .
Page 8 ADVIA 2120/2120i Operator’s Guide Atypical Lymphocytes (ATYP) ......423 Blasts (BLASTS)........425 HGB Concentration Variance (HCVAR).
Page 9 ADVIA 2120/2120i Operator’s Guide Retic – Plt Interference Error (RTCint, CT) ....470 Retic Absorption Distribution Abnormal (RTCADA, CA) ..470 Retic Absorption Flatness (RTC-FL, RF) .
Page 10 ADVIA 2120/2120i Operator’s Guide Anisocytosis - Alarm........489 Anisocytosis - Stop .
Page 11 ADVIA 2120/2120i Operator’s Guide Autosampler - Centering Collar Failed to Extend, Reset Autosampler......... 500 Autosampler - Centering Collar Failed to Retract, Reset Autosampler.
Page 12 ADVIA 2120/2120i Operator’s Guide Autosampler - Needle Failed to Extend, Reset Autosampler..515 Autosampler - Needle Failed to Retract, Reset Autosampler..516 Autosampler - Needle Jammed ......517 Autosampler - Needle Motion Denied, Reset Autosampler.
Page 13 ADVIA 2120/2120i Operator’s Guide BASO Reagent Expired ....... . . 532 BASO Reagent Low - Alarm .
Page 14 ADVIA 2120/2120i Operator’s Guide GETEQUAL ......... . 543 GlobalLock or GlobalUnlock.
Page 15 ADVIA 2120/2120i Operator’s Guide Large Platelets - Stop ........553 Laser Power Low - Alarm .
Page 16 ADVIA 2120/2120i Operator’s Guide Non WBC Interference - Stop ......561 Nucleated Red Blood Cells - Alarm......562 Nucleated Red Blood Cells - Stop .
Page 17 ADVIA 2120/2120i Operator’s Guide PEROX Temperature Out - Stop ......573 Platelet Clumps - Alarm ....... . 573 Platelet Clumps - Stop .
Page 18 ADVIA 2120/2120i Operator’s Guide RETIC Noise Origin - Alarm......584 RETIC Noise Origin - Stop ....... 584 RETIC - PLT Interference/Error - Alarm .
Page 19 Basophil / Lobularity Temperature Control ....601 ADVIA 2120/2120i BASO Reagent ......601 ADVIA 2120/2120i SHEATH / RINSE.
Page 20 Cytochemical Reactions ....... . 647 ADVIA 2120/2120i RBC / PLT ......647 Measurement .
Page 21 Cytochemical Reactions ....... . 673 ADVIA 2120/2120i autoRETIC ......673 Measurement.
Page 22 ADVIA 2120/2120i Operator’s Guide Running Body Fluid Specimens Using the MOTS and MCTS ..703 Running Body Fluid Specimens Using the Autosampler ..704 Materials Provided ....... . 704 Materials Required but not Provided .
Page 23 ADVIA 2120/2120i Operator’s Guide System Method Information: Procedure ....725 System Method Information: Quality Control ....726 System Method Information: Gain Adjustment .
Page 24 ADVIA 2120/2120i Operator’s Guide Expected Values ........749 Performance Characteristics: Precision .
Page 25 ADVIA 2120/2120i Operator’s Guide Performance Characteristics: Analytical Range ....770 WBC Data Summary....... 771 CBC Method Data.
Page 26 ADVIA 2120/2120i Operator’s Guide Sample / System Flags ....... . . 801 Doctor.
Page 27 ADVIA 2120/2120i Operator’s Guide Appendix A: Legal Information Serious Incident Reporting ......857 Limited Instrument Warranty and Service Delivery Policy.
Page 28 ADVIA 2120/2120i Operator’s Guide Appendix C: Orderable Parts Consumable Parts........877 Customer Replaceable Parts .
Page 29 This technical information is not part of the instructions for use, and is intended for the information technology or security professional. The ADVIA 2120/2120i Hematology Systems are for professional use only. 10708774 Rev. 04...
Page 30: Summary And Explanation
Summary and Explanation The ADVIA 2120 and ADVIA 2120i Hematology Systems with Autoslide are for use to identify patients with hematologic parameters within and outside of established reference ranges.
Page 31 ADVIA 2120/2120i Operator’s Guide Overview Input queue (Autosampler Sample, sheath, and inside) diaphragm pumps Manual open-tube sampler Reagents, sheath, wash, and rinse Manual closed-tube sampler 10 Vacuum and pressure regulator knobs Unifluidics technology 11 Touchpad Perox optical assembly 12 Manual barcode reader...
Page 32 Overview ADVIA 2120/2120i Operator’s Guide Table 1: Allowable tube sizes Tube size (mm) Manufacturer Draw Minimum Vol. diameter x Vol. Required height (mL) Dead Vol. (mL) 10 x 50 Becton-Dickinson – VACUTAINER 10 x 64 Venoject – 11 x 65...
Page 33 You must use the additional Sarstedt carrier tube with the 1.2 mL Sarstedt Monovette tubes. The 1.2 mL tube is significantly smaller in dimension and is required for use the ADVIA 2120/2120i Hematology system. Some of the allowable tube closure types: •...
Page 34 Overview ADVIA 2120/2120i Operator’s Guide Unified Fluids Circuit (UFC) The UFC assembly uses Unifluidics ™ technology. Hgb Reaction Chamber Perox Reaction Chamber (Not Visible) Baso Reaction Chamber Shear Valve RBC Reaction Chamber Reagent Pump Assembly Retic Reaction Chamber 10708774 Rev. 04...
Page 35 ADVIA 2120/2120i Operator’s Guide Overview Shear Valve Assembly The shear valve is made up of two ceramic disks. The rear disk is stationary. The front disk rotates to shear or divide the sample into appropriate aliquots for analysis. The front disk also rotates to allow aspiration for direct cytometry.
Page 36 Overview ADVIA 2120/2120i Operator’s Guide Reaction Chambers The reaction chambers are machined pockets in the UFC assembly where the sample and reagents are mixed and the cytochemical reaction takes place. Hgb reaction chamber (not visible) Baso reaction chamber RBC reaction chamber...
Page 37 ADVIA 2120/2120i Operator’s Guide Overview Optical Assemblies The perox optical assembly directs light from a tungsten-halogen lamp to the perox flowcell. The use of a sheath stream in the flowcell allows a cell- by-cell measurement of light scatter and absorption.
Page 38 Overview ADVIA 2120/2120i Operator’s Guide Perox Optical Assembly The perox optical assembly measures scattering and absorption of a tungsten halogen lamp as the scattering and absorption passes through a stream of prepared white blood cells in a flowcell. A Tungsten halogen lamp...
Page 39 ADVIA 2120/2120i Operator’s Guide Overview Laser Optical Assembly The laser optical assembly consists of the illuminator, flowcell, and detector assemblies. A laser diode, housed in the illuminator assembly, is used as the light source. Illuminator assembly Flowcell location Detection assembly The image of a slit illuminated by light from the laser diode is focused into the flowcell.
Page 40 Overview ADVIA 2120/2120i Operator’s Guide Laser diode driver board Absorption detector Laser diode Asymmetrical dark stop Sample stream Low angle scatter detector Hi-NA lens Beam divider Beam splitter High angle scatter detector 10708774 Rev. 04...
Page 41 ADVIA 2120/2120i Operator’s Guide Overview Hemoglobin Colorimeter Assembly The hemoglobin colorimeter assembly straddles the UFC block (C) at the top. A Lamp assembly B Hgb reaction chamber C UFC assembly The hemoglobin colorimeter contains a light source set at 3.5 Vdc and a 565 nm or 546 nm interference filter, according to the HGB method selected.
Page 42 Overview ADVIA 2120/2120i Operator’s Guide Sample, Sheath, and Diaphragm Pumps The perox sample and sheath pumps are located on the left side of the analyzer. Perox sample pump Sheath pumps RBC/baso/retic sample pump Sheath pump The RBC/baso/retic sample and sheath pumps are located on the right side of the analyzer.
Page 43 ADVIA 2120/2120i Operator’s Guide Overview The diaphragm pumps for sheath, rinse, and wash (5 in all) are located above the reagent containers. Sample pump Sheath Reacted sample Sheath filters Reaction chamber Sheath pumps Flowcells Reacted sample stream Sheath diaphragm pumps...
Page 44 Overview ADVIA 2120/2120i Operator’s Guide Reagents, SHEATH / RINSE, Wash, and DEFOAMER Reagent is mixed with a sample for cytochemical analysis and measurement. Sheath is a fluid that envelops the sample stream as the sheath passes through the optics, ensuring cell-by-cell analysis.
Page 45 ADVIA 2120/2120i Operator’s Guide Overview Touchpad The touchpad, located on the lower right of the analyzer, is the means by which you operate the analyzer. Eject rack Standby Rack in sampler Start / Stop sampler Power off • Provides power to the analyzer assemblies and power supplies. After pressing On, samples through the manual open- and closed-tube samplers can be processed within 2.5 minutes, while samples through...
Page 46 Overview ADVIA 2120/2120i Operator’s Guide On the touchscreen monitor: • Use your finger, with or without gloves, to select all window controls, including function buttons and operations in the online Help. • Move a window by holding your finger on the title bar of the window, and dragging the window to the new location.
Page 47 ADVIA 2120/2120i Operator’s Guide Overview Waste removal system All analyzer waste is evacuated to an 11-liter waste container that can be stored in a stand-alone container, which must be emptied manually, or part of the automatic waste removal system. When the fluid level in either...
Page 48 Overview ADVIA 2120/2120i Operator’s Guide How the ADVIA 2120/2120i Hematology System Works Blood samples can be aspirated through the: • Autosampler • Manual closed-tube sampler • Manual open-tube sampler After a sample is aspirated, the sample is drawn into the shear valve. As the shear valve rotates, the shear valve shears or divides the sample into aliquots for different types of tests.
Page 49: Table Of Contents
Test results are sent to the computer to be reviewed and edited. How the ADVIA 2120/2120i Software Works The ADVIA 2120/2120i system software is a special-purpose program that runs on the Windows operating system. Use the mouse to navigate through the software and operate the system.
Page 50: Status Lines
Overview ADVIA 2120/2120i Operator’s Guide Status lines There are two status lines that display messages about the system. Both lines consist of 2 parts, each displaying different information. The system saves all messages in the message log. Select the message icon to view the last 10 messages.
Page 51: Menus
ADVIA 2120/2120i Operator’s Guide Overview Second Status Line Icon Description Left side Current system state; for example, Ready to Run. Right side System messages and icons that indicate the message severity. Information accompanies a message that provides information only. No operator action is necessary.
Page 52: Main Display Area
Help button When you need detailed Help that provides step-by-step instructions for operating the ADVIA 2120/2120i, select the Info button. To get brief (What's This) Help, select the ? icon on the status line or press the Shift and F1 keys at the same time, then select the item for which you want help.
Page 53 ADVIA 2120/2120i Operator’s Guide Overview Results The ADVIA 2120/2120i system can run 5 types of whole blood selectivities: CBC, CBC/DIFF, RETIC, CBC/RETIC, and CBC/DIFF/RETIC. Select these tests by: • Setting the selectivity to default • Requesting the type from the Manual Sample ID tab •...
Page 54 Overview ADVIA 2120/2120i Operator’s Guide Specifications Models • ADVIA 2120/2120i Hematology System with Dual Aspirate Autosampler (Model 11219529) • ADVIA 2120/2120i Hematology System with Single Aspirate autosampler (Model 11219530) • ADVIA 2120/2120i Hematology System without Autosampler (Model 11219531) • ADVIA 2120/2120i Hematology System with Autoslide Slide Maker Stainer (Model 10282177) •...
Page 55: Data Management
ADVIA 2120/2120i Operator’s Guide Overview Data Management • Database storage capacity of 10,000 records, including graphics • Review and edit capability User-defined windows ○ User-defined reports ○ User-defined ranges based on age and sex for Normal, Rerun, Panic, ○ and Delta Check criteria •...
Page 56 Overview ADVIA 2120/2120i Operator’s Guide Parameters CBC Results WBC, RBC, HGB, HCT, MCV, MCH, MCHC, CHCM, RDW, HDW, CH, CHDW, PLT Differential Results NEUT, LYMPH, MONO, EOS, BASO, LUC (Large (absolute and %) Unstained Cells) Platelet Results PLT, MPV, PDW, PCT...
Page 57: Performance Specifications
ADVIA 2120/2120i Operator’s Guide Overview Performance Specifications Analytical Ranges (Linearity) 0.02–400 x 10 /µ 0.0–7.0 10 /µ 5.0–3500 X 10 /µ 0–22.5 g/dL Retic 0.2–24.5% Within Run Precision Mean 0.06 15.0 0.14 0.93 0.78 2.93 Retic% 0.25 12.5 Carryover < or = to 1% for all parameters...
Page 58 Azide-free reagents drain into the waste container with automatic level-sensor shutoff. Waste per CBC/diff.retic cycle, including rinse 23 The ADVIA 2120/2120i Hematology System is for indoor use only. Operation of the instrument at altitudes of over 2000 meters (6000 feet) is not recommended.
Page 59: Turn On And Off
2 Turn On and Off Overview The ADVIA 2120/2120i Hematology system consists of 2 main components: computer and analyzer. Although there is a main power switch for the system, you must also power on and off each component individually. Powering On the System Check that the main power switch is set to On.
Page 60 Turn On and Off ADVIA 2120/2120i Operator’s Guide 5. Turn on the Analyzer. NOTE: When restarting the analyzer, ensure at least 60 seconds have elapsed since powering off the analyzer. a. Select On at the analyzer touchpad. b. When the computer finishes loading the software, the Log On/Off tab displays.
Page 61: Powering Off The System
CAUTION You must follow the proper procedure when shutting down the ADVIA 2120/2120i Hematology System. Failure to follow the proper procedure can result in corruption of the database. Turning the system off: In an Emergency 1. Select Off at the analyzer touchpad.
Page 62 Wait 1–2 minutes while the software shuts down. 3. Set the computer power switch to Off. Exiting the ADVIA 2120/2120i Software Exit the ADVIA 2120/2120i software without turning off the computer power: 1. At the Operations menu, select Log On / Off.
Page 63: Working With The Software
Windows 2000 functions. Select Logoff only if the system software does not start properly and you cannot shut down directly from the ADVIA 2120/2120i LogOn / Off tab. Supervisor When you log on to Windows 2000 using a supervisor name and password, the supervisor shell opens.
Page 64: Time And Date
6. Log on to the system using an operator password. To start the ADVIA 2120/2120i software directly from the ADVIA 2120/2120i Shell, select ADVIA 2120/2120i. If you do so, the Supervisor or Field Service permissions will remain active. 10708774 Rev.04...
Page 65: Adding A Printer
ADVIA 2120/2120i Operator’s Guide Working with the Software Exiting the ADVIA 2120/2120i Software To exit the ADVIA 2120/2120i software without turning off the computer power: 1. At the Operations menu, select Log On / Off. 2. Select Shut Down ADVIA.
Page 66: Setting Paper Size
Select OK. Setting up the system To set up the system so you can create a file: 1. Exit the ADVIA 2120/2120i software. 2. In the Logon Information box, log on to the system using a supervisor or field service password.
Page 67: Creating The File
ADVIA 2120/2120i Operator’s Guide Working with the Software 12. If the driver is already installed, you will be asked if you want to keep the driver. 13. Ensure Keep existing driver is selected. 14. Select Next >. • If the driver is not installed, contact your local technical support provider for assistance.
Page 68: Turning On The Analyzer
4. To log off the system, select Log Off. Exiting the system: 1. Turn off the system. 2. Exit the ADVIA 2120/2120i software without turning the computer power off. Standby Mode Place the analyzer in the Standby mode during periods of inactivity.
Page 69 Once you have logged on to Windows using an operator name and password, the computer automatically starts the software. NOTE: If you log on using a supervisor name and password, the system opens the ADVIA 2120/2120i Shell. 5. Turn on the analyzer. Startup Tab...
Page 70 Working with the Software ADVIA 2120/2120i Operator’s Guide 7. Optional: Select Startup Log to view previous startup information. 8. Optional: On systems equipped with an ADVIA® Autoslide Slide Maker Stainer, select the Autoslide button to view startup information for the autoslide module.
Page 71 ADVIA 2120/2120i Operator’s Guide Working with the Software If any result is out of range, select Refresh at the Startup tab to run another background count cycle. The refresh button is unavailable when the system is in Standby mode. If any result is still unacceptable, perform a system wash.
Page 72 The sample selectivity displays on the first status line. Running CSF Samples To run CSF samples on your ADVIA 2120/2120i Hematology System, perform the following procedure: 1. If the Standby indicator is lit, press the Standby button to put the system into Ready to Run mode.
Page 73 10. After appropriate incubation, mix the sample again, either by inverting it 5-10 times or by vortexing it for 5 seconds immediately before aspiration on the ADVIA 2120/2120i Hematology System. Cells in prepared CSF samples will settle quickly due to low viscosity.
Page 74 Working with the Software ADVIA 2120/2120i Operator’s Guide 11. Aspirate the sample: a. Position the tube so that the sample probe is immersed into the well-mixed sample. The sample probe should not contact test tube surface, which could block the free flow of sample into the probe.
Page 75 Working with the Software Body Fluids Method: Running Body Fluid Specimens (For use with ADVIA 2120/2120i systems only) NOTE: Ensure adequate sample volume. A minimum of 1 ml is required for the Manual Closed Tube Sampler (MCTS) and Autosampler. At least 300 µL is needed for the Manual Open Tube Sampler (MOTS).
Page 76 Working with the Software ADVIA 2120/2120i Operator’s Guide 8. At the green aspirate light, aspirate the well-mixed and particulate-free body fluid sample through the MOTS or MCTS. NOTE: Ensure that the MOTS sample probe is completely immersed in the sample, especially when the sample volume is low. Most body fluid samples have less viscosity and color compared to whole blood samples resulting in very fast aspiration that may not be visible.
Page 77 ADVIA 2120/2120i Operator’s Guide Working with the Software To view the run status: 1. From the Autosampler run list box located in the upper left corner of the window, select the run you want to view. Each run is identified by its starting date and time.
Page 78 Working with the Software ADVIA 2120/2120i Operator’s Guide The Service History window enables you to view and print a cumulative record of all service activities for a specific period. Select Service History to view the window. Service History Window The following information displays for each procedure: The Service History window enables you to view and print a cumulative record of all service activities for a specific period.
Page 79 ADVIA 2120/2120i Operator’s Guide Working with the Software Unscheduled Service Window The following information displays for each unscheduled service procedure: Procedure User Code Date Performed Comments To enter an unscheduled intervention: 1. Select New Entry. 2. In the dialog box, select or define the procedure to add to the service log.
Page 80 Working with the Software ADVIA 2120/2120i Operator’s Guide Service History Window The following information displays for each procedure: Procedure User Code Logged Sample Count Category Sample Interval Intervention Type Due Date Comments Date Performed To search the service log: The History Query dialog box displays when you open the Service History window.
Page 81 ADVIA 2120/2120i Operator’s Guide Working with the Software Exporting the Service History Log to a File NOTE: Do not attempt to export a log while the system is processing samples. 1. Select Export to open the Export to File window.
Page 82: Deleting Messages
Working with the Software ADVIA 2120/2120i Operator’s Guide The system searches the log and displays the messages. Searching Message Log by Message Status Level 1. Select Message Status. 2. Select one or more message status levels. 3. Select OK. The system searches the log and displays the messages.
Page 83 ADVIA 2120/2120i Operator’s Guide Working with the Software To delete multiple messages: 1. Select the first message you want deleted. 2. Remove the empty TIMEPACou have the following options: • Hold down the Shift key while selecting the last message to delete.
Page 84 Working with the Software ADVIA 2120/2120i Operator’s Guide 3. Select OK when asked to confirm. Exporting the Message Log to a File NOTE: Do not attempt to export a log while the system is processing samples. To export the entire Message Log to a file: 1.
Page 85: Reagent Inventory Window
ADVIA 2120/2120i Operator’s Guide Working with the Software Reagent Inventory Window The following information displays for each of the current reagents: Cycles Remaining Days Remaining Alarm Criteria Lot # Stop Criteria Expiration Date To prime a specific reagent line: 1. Select the name of the reagent line you want to prime.
Page 86 Working with the Software ADVIA 2120/2120i Operator’s Guide NOTE: A new CUBITAINER filter is packaged with every container of SHEATH/RINSE. 5. Replace the CUBITAINER filter at the end of the straw and immediately insert the assembly into the new bottle.
Page 87 ADVIA 2120/2120i Operator’s Guide Working with the Software 12. Alternately, enter the information by typing in the data. NOTE: If the new reagents are a different lot number, the gains may need to be adjusted and the channels recalibrated. 13. Run a whole-blood control.
Page 88 Working with the Software ADVIA 2120/2120i Operator’s Guide 2. Remove the straw/filter assembly or individual straw from the bottles to be replaced. To avoid contamination, be careful that droplets do not splatter from the end of the straws. 3. Remove the empty TIMEPAC® or bottle from the analyzer and discard it in an environmentally safe manner.
Page 89 ADVIA 2120/2120i Operator’s Guide Working with the Software 13. If bubbles remain in the reagent lines, prime the lines again by using the Reagent Inventory window. 14. Alternately, enter the information by typing in the data. NOTE: If the new reagents are a different lot number, the gains may need to be adjusted and the channels recalibrated.
Page 90 Working with the Software ADVIA 2120/2120i Operator’s Guide Reagent locations DIFF TIMEPAC CBC TIMEPAC autoRETIC EZ Wash DEFOAMER SHEATH/RINSE is in a 10-L or 20-L CUBITAINER A liquid-level monitoring alarm alerts you to replace the SHEATH/RINSE. All other reagents, except DEFOAMER, have a cycles remaining alarm.
Page 91 ADVIA 2120/2120i Operator’s Guide Working with the Software Open-bottle Stabilities (59°F to 86°F) (15°C to 30°C) BASO 90 days 30 days 90 days CN-free HGB 45 days RBC/PLT 45 days PEROX 1 90 days PEROX 2 90 days PEROX 2...
Page 92 Working with the Software ADVIA 2120/2120i Operator’s Guide 3. To confirm select OK. 4. When prompted to prime lines, select Yes. 5. Enter the necessary number of prime cycles, then select OK. 6. The system primes the lines for the new reagent and all associated reagents.
Page 93 ADVIA 2120/2120i Operator’s Guide Working with the Software Cumulative Inventory Window To determine the number of reagent containers used over a specific period of time: 1. In Start Date box, enter in the beginning date directly, or select the down arrow, then select the date from the calendar.
Page 94 Working with the Software ADVIA 2120/2120i Operator’s Guide Exporting the Startup Log to a File Do not attempt to export a log while the system is processing samples. 1. Select Export to open the Export to File window. 2. At the Export to File window, select a drive and, if necessary, a subdirectory to export the log to.
Page 95 ADVIA 2120/2120i Operator’s Guide Working with the Software Searching for Notes by Date 1. Select QC Comments or User Notes. 2. Enter the starting date for the search. 3. Enter the ending date for the search. Searching for Notes by Date 1.
Page 96 Working with the Software ADVIA 2120/2120i Operator’s Guide 3. You can: • Select the Date / Time table heading to sort notes chronologically. The most recent note displays first. • Select the User Code table heading to sort notes alphanumerically by the logon user code.
Page 97 ADVIA 2120/2120i Operator’s Guide Working with the Software 3. In the Export - All or Selected Records list, select All. 4. Select Start. Export the selected entries to a file 1. In the QC Comments list or User Notepad, select the entries to export.
Page 98 Working with the Software ADVIA 2120/2120i Operator’s Guide Searching for a Slide You can search for slides in the Slide Log using one of four available methods: • Aspiration Date and Time • Sample ID • Rack and Position Number •...
Page 99 ADVIA 2120/2120i Operator’s Guide Working with the Software Deleting Slide Log Entries You can delete all the entries in the slide log or selected entries. 1. From the pull-down menu next to the Delete button, select the deletion method to use: All or Selected.
Page 100: Utilities Menu
Working with the Software ADVIA 2120/2120i Operator’s Guide Utilities Menu Analyzer Status Tab Analyzer Status tab for systems with v 6.10.9 and higher. Vacuum and Pressure Readings Each gauge displays a current vacuum or pressure reading on a color- coded background. The correct value and acceptable set-point tolerance display above each gauge.
Page 101 ADVIA 2120/2120i Operator’s Guide Working with the Software The UFC "Hg gauge indicates the vacuum in the UFC. If the reading displays on a red background when the system is idle, the difference between the compressor vacuum and the UFC vacuum is greater than 5-inch Hg. Check for a loose cap or fittings on the waste container and check the vacushield filter for a leak or clog and replace it filter if necessary.
Page 102 Working with the Software ADVIA 2120/2120i Operator’s Guide Turn knobs clockwise to increase values and counterclockwise to decrease values. You have to first pull the 20-psi and 40-psi pressure regulator knobs, then turn to adjust. Regulator Acceptable Reading Set to 20 in.
Page 103 ADVIA 2120/2120i Operator’s Guide Working with the Software • The correct temperature and acceptable range display next to each thermometer. NOTE: The system continually updates the readings to reflect changes while running samples or hydraulic cycles. These fluctuations are normal and do not indicate an analyzer malfunction.
Page 104 Working with the Software ADVIA 2120/2120i Operator’s Guide Hydraulic Functions Tab Use the Hydraulic Functions tab to start one of the analyzer’s hydraulic operations.The analyzer must be in the Ready-to-Run mode. On systems equipped with an ADVIA Autoslide Slide Maker Stainer, select the Autoslide button to open the Hydraulic Functions tab for the autoslide module.
Page 105 ADVIA 2120/2120i Operator’s Guide Working with the Software 2. Enter the number of consecutive cycles to run. • The range is 1 to 15. • Use the up arrow in the Number of Cycles area to increase the number. •...
Page 106 Regular strength household bleach is 5% sodium hypochlorite. Extra- strength household bleach is 6% sodium hypochlorite. Either strength may be used with the ADVIA 2120/2120i Hematology System. When using or handling ADVIA EZ Wash, bleach, any cleaning or antiviral agent, or any other potentially hazardous liquids, wear protective clothing, gloves, and safety glasses.
Page 107 Semi-automatic Hydraulic Pathway Wash NOTE: Recommended frequency to conduct the Vent Line Wash is monthly. Performing the semi-automatic hydraulic pathway wash: 1. Let one bottle of ADVIA 2120/2120i Vent Line Wash stand for 15–20 minutes to come to room temperature. 10708774 Rev.04...
Page 108 Working with the Software ADVIA 2120/2120i Operator’s Guide 2. Open the bottle of ADVIA 2120/2120i Vent Line Wash and remove the vent overflow tubing from overflow bottle. 3. Insert tubing into the RBC and Perox shuttle chamber vent line fittings.
Page 109: Indicators - Lights
ADVIA 2120/2120i Operator’s Guide Working with the Software Supply Voltage Colorimeter voltage Hgb Chamber Reading Voltage reading from the empty chamber Indicators - Lights Test the lights: 1. Select the circle representing a light. The circle turns green and the corresponding light on the analyzer should go on.
Page 110 Working with the Software ADVIA 2120/2120i Operator’s Guide Sensors This section of the Indicators window indicates the status of the following sensors: Waste Container The indicator reads “not full” unless the waste container level is above the sensor. SHEATH/ RINSE The indicator reads “not empty”...
Page 111 ADVIA 2120/2120i Operator’s Guide Working with the Software Voltages Voltage Acceptable Range +5.1 Volts Switched 4.92 to 5.270 +5.1 Volts Unswitched 4.87 to 5.330 +12.1 Volts Switched 11.20 to 13.010 -12.1 Volts -11.20 to -13.010 +24.0 Volts 22.46 to 25.580 +15.1 Volts Switched...
Page 112 Working with the Software ADVIA 2120/2120i Operator’s Guide Valves - Hydraulic and Dome Valves 1. Select a valve symbol to open its corresponding hydraulic or dome valve. The valve symbol will change to indicate its position. Open Closed Fluid can move through the valve Fluid cannot move through the valve 2.
Page 113 ADVIA 2120/2120i Operator’s Guide Working with the Software Pneumatics Sample Shear Valve Test the sample shear valve: 1. If the compressor is not on, at the Compressor section of the Pneumatics window, select the Turn On button. The sample shear valve will be in the aspirate position.
Page 114 Working with the Software ADVIA 2120/2120i Operator’s Guide Probe Sleeve (wash block) Test the probe sleeve: 1. If the compressor is not on, at the Compressor section of the Pneumatics window, select the Turn On button. 2. Select the probe sleeve symbol to lower the probe sleeve (wash block) mechanism.
Page 115: Syringe Pumps
ADVIA 2120/2120i Operator’s Guide Working with the Software Compressor To turn the compressor on, select Turn On. To turn the compressor off, select Turn Off. The vacuum and pressure indicators display the current readings. The correct value and acceptable tolerance displays above each indicator.
Page 116: Selector Valve
Working with the Software ADVIA 2120/2120i Operator’s Guide 5. Select the Home button to move the pump through its range of motion and back to the home position. The pump moves and the symbol changes to reflect its position. Each pump symbol displays on a green background when you open the Syringe Pumps window.
Page 117 ADVIA 2120/2120i Operator’s Guide Working with the Software Centering Collar The centering collar holds the top of the tube so the needle punctures the center of the stopper. Input Queue The input queue is the area where the operator puts racks so they can be processed by the system using the Autosampler.
Page 118: Utilities Menu
Working with the Software ADVIA 2120/2120i Operator’s Guide Buttons Enter Diagnostic Mode Puts the Autosampler in diagnostic mode. Enter Operating Mode Puts the Autosampler in the operating mode. The Autosampler must be in operating mode before you select Autosampler Start or Eject Racks.
Page 119 ADVIA 2120/2120i Operator’s Guide Working with the Software NOTE: The customizable font and type size can affect the amount of data that will be visible on the Run Screen. Ensure the Run Screen configuration meets your printing needs and includes all necessary information before running patient samples.
Page 120 Working with the Software ADVIA 2120/2120i Operator’s Guide In addition to the items available on the menu bar, you can also select the Run Screen to display another menu. This menu has these options: • Configure • Save Positions Viewing an Existing Configuration By selecting Open on the menu bar, you can view sample information in any screen configuration.
Page 121 ADVIA 2120/2120i Operator’s Guide Working with the Software 4. Select an entry in the list. You can select more than one. You can select any entry again to deselect the entry. • Select Add to add the selected parameter windows to the configuration.
Page 122 Working with the Software ADVIA 2120/2120i Operator’s Guide c. If you want to associate the configuration with a selectivity, select the Associate Run Screen checkbox and then select the appropriate selectivity from the pull-down list. This causes the system to use this configuration as the default for all samples with the associated selectivity.
Page 123 ADVIA 2120/2120i Operator’s Guide Working with the Software • To create a new header, enter the name of the new header in the Window Title field and then select Add New. Names used for headers are not case sensitive. To avoid overwriting an existing file, make sure your file name is unique.
Page 124 Working with the Software ADVIA 2120/2120i Operator’s Guide NOTE: The customizable font and type size can affect the amount of data that is visible on the Run Screen. Ensure the Run Screen configuration meets your printing needs and includes all necessary information before running patientsamples.
Page 125 ADVIA 2120/2120i Operator’s Guide Working with the Software Delete the old window name, enter the name of the new ○ window, and then select Add New. The new window will contain the same fields as the window ○ you copied.
Page 126 Working with the Software ADVIA 2120/2120i Operator’s Guide 5. Completing the parameter window: • To save the parameter window and close the Header/Parameter Configuration window, select OK. • To save the parameter window and leave the Header/Parameter Configuration window open, select Apply.
Page 127 ADVIA 2120/2120i Operator’s Guide Working with the Software NOTE: If necessary, you can restore the default settings of the system's defaultparameter windows. Select the parameter window you want to format from the Window Title pull-down menu. Select Restore Defaults and then select Apply or OK to save the settings.
Page 128: Backup/Restore Tab
Working with the Software ADVIA 2120/2120i Operator’s Guide • To return to the Run Screen, select the Run Screen tab on the Utilities menu. Turn off the Run Screen display: Select Display Off on the Run Screen menu bar. The Run Screen will not display again until you select the Run Screen tab on the Utilities menu.
Page 129 ADVIA 2120/2120i Operator’s Guide Working with the Software • If you want to delete the entire subdirectory, select the subdirectory you want to delete. • If you want to delete only some of the files in a subdirectory, select individual data files from the Select Files list or select Select All Files to select them all.
Page 130 Working with the Software ADVIA 2120/2120i Operator’s Guide 5. When the system prompts you, insert the first backup disk and select If more than one disk is necessary, the system prompts you again for additional disks. NOTE: Ensure you use new or freshly formatted disks.
Page 131 Working with the Software 2. After the system has completed the repair process, you must shut down the ADVIA 2120/2120i software and restart the computer for the repair to take effect. NOTE: If you do not restart the computer immediately...
Page 132 Working with the Software ADVIA 2120/2120i Operator’s Guide 4. Each time you aspirate the OPTIpoint, slowly move the alignment micrometer knob in one direction until the appearance of the cytogram indicates that the flowcell is properly aligned. If the cytogram moves further out of alignment, turn the knob in the other direction.
Page 133 ADVIA 2120/2120i Operator’s Guide Working with the Software 5. When you have aligned the flowcell, run another aspiration (repeat steps 2–3), without making any thumb wheel adjustments, to confirm the alignment. The cytogram should continue to indicate proper alignment. •...
Page 134 Working with the Software ADVIA 2120/2120i Operator’s Guide To adjust the gains: Using the Gain Wizard: Select Gain Wizard, then follow the instructions. Adjusting gains without the Gain Wizard: 1. Set up the adjustment using the Gain Definition window. 2. Enter reference information for the gain samples in the Reference Data window.
Page 135 ADVIA 2120/2120i Operator’s Guide Working with the Software Installing reagents with a different lot number. ○ When indicated by quality control data, or frequent false positive or ○ false negative morphology flags. What to check before adjusting gains Perform the following before adjusting gains: 1.
Page 136 Working with the Software ADVIA 2120/2120i Operator’s Guide Gain Reference Data You can enter reference data using either the barcode reader or the keyboard. To enter reference data using the barcode reader: 1. Enter values for highlighted parameters only. 2. Hold the barcode reader against the barcode label on the ADVIA OPTIpoint or ADVIA SETpoint®...
Page 137 ADVIA 2120/2120i Operator’s Guide Working with the Software To accept or reject gain factors: • If the gain factors procedure has passed, the word Passed displays in the Gain Status box and in the Status column beside each parameter. Accept all gain factors.
Page 138 Working with the Software ADVIA 2120/2120i Operator’s Guide Gain Acceptance (final acceptance) NOTE: You must accept gain factors before they can be used. NOTE: Because the system deletes the gain data points after you select Finished, open the Run / Data Review window and select Print Report if you want to print them.
Page 139 ADVIA 2120/2120i Operator’s Guide Working with the Software To position the cell signatures within the normal cytogram regions, the gain factors have to be increased or decreased manually. To adjust the gain factors, at the Adjust Gains tab, select the Gain Factors button.
Page 140 Working with the Software ADVIA 2120/2120i Operator’s Guide To omit gain sample data: A check in the box in the Apply column indicates that the row is included in the data set. Select the box to remove the check and omit the row. You cannot omit a single data point;...
Page 141 2. Enter reference information for the calibration samples at the Calibration Reference window. 3. Run the calibration samples on the ADVIA 2120/2120i system using the primary sampler. When you calibrate Baso WBC and Perox WBC, also aspirate samples using the other samplers to calibrate the parameters across all modes of aspiration.
Page 142 Working with the Software ADVIA 2120/2120i Operator’s Guide • If there is a significant shift in control values after: Replacing a critical hydraulic or optical component ○ Installing reagents with a different lot number ○ • Any time commercial control products and/or moving averages are out...
Page 143: Calibration Factor
CLSI or ICSH or laboratories using certain flow cytometric methods, could observe a significant bias when comparing to the ADVIA 2120/2120i method. In this event, use the calibration procedure to obtain agreement with the other method. Calibration Factor The number obtained from the calibration procedure that adjusts the results to agree with another analyzer or a reference method.
Page 144 Working with the Software ADVIA 2120/2120i Operator’s Guide Use the Calibration tab of the Procedures menu to recalibrate the ADVIA 2120/2120i system. 10708774 Rev.04...
Page 145 Enter the MCHC value in the CHCM box if using a method or instrument that is not distributed by the local authorized service provider or distributor to calibrate the ADVIA 2120 / 2120i Hematology System. • Use the up and down arrow keys to move from field to field.
Page 146 Working with the Software ADVIA 2120/2120i Operator’s Guide Using the keyboard to enter data for commercial calibration: 1. Enter in the Expiration Date and Lot # from the calibrator product insert. 2. In the Ref Values boxes, enter the System Specific Values (SSV) from the calibrator product insert.
Page 147: Data Review
ADVIA 2120/2120i Operator’s Guide Working with the Software • If calibration has failed, “Failed” displays in the Calibration Status box when at least one parameter fails the precision specification limits, cal factor range check, or has insufficient data points. •...
Page 148 Working with the Software ADVIA 2120/2120i Operator’s Guide Calibration Factors Log To change an existing cal factor: 1. Position the cursor in the column next to the cal factor you want to change. 2. Enter a new value for the cal factor.
Page 149 ADVIA 2120/2120i Operator’s Guide Working with the Software To adjust the Hgb baseline: 1. Select Start Cycle. 2. A/D values display after the Hgb reaction chamber is filled with SHEATH/RINSE. • If Current value is between 3.9 and 4.1, proceed to step 3.
Page 150 Working with the Software ADVIA 2120/2120i Operator’s Guide 2. Check temperature reading. • If the Baso temperature value is between 32°C and 34°C, proceed to step 4. • If not within range, adjust the perox reaction chamber temperature. 3. Select Exit to return to the Adjust Lamp / Temp Introduction window.
Page 151: Customize Menu
Most of the windows are available in Tools View and Tools Modify. NOTE: Windows in the Tools look and behave differently from the rest of the ADVIA 2120/2120i software. They contain a menu bar of their own and you must manually close each window before you open another window or tab.
Page 152 If you select the Incomplete check box, you will get a sample list corresponding to the Incomplete status, including any Unmatched samples. Run number Not for use with the ADVIA 2120 Hematology System. Date/Time Area • Extracts all the samples for the selected list regardless of creation or aspiration date and time.
Page 153 ADVIA 2120/2120i Operator’s Guide Working with the Software • Format Box Lists the formats available for displaying or printing the selected list. Formats must have been previously defined in the Format window. The system will not generate a list if the format does not exist or is improperly defined.
Page 154 Working with the Software ADVIA 2120/2120i Operator’s Guide The content of each list depends on the format defined in the Format window. The list can contain: • A header inserted at the beginning of a page or screen, including: type of list ○...
Page 155 ADVIA 2120/2120i Operator’s Guide Working with the Software • The bottom of a page can indicate either the page number or the same type of information that is displayed in the header. Selecting a Sample List 1. Select the check boxes corresponding to the sample lists you want.
Page 156 Working with the Software ADVIA 2120/2120i Operator’s Guide 2. Select the Date/Time option you want. If you select Partial, enter the required information in the From and To fields, or, if applicable, use the default date and time displayed. Use the mouse or arrow keys.
Page 157 ADVIA 2120/2120i Operator’s Guide Working with the Software Work Area • Run Parameters Routine Sample Type ○ Hold Status ○ Position Mode ○ • Host Transmission • Report Routine Sample Type Identifies the sample associated with the Order Entry tab.
Page 158 Working with the Software ADVIA 2120/2120i Operator’s Guide • Position Mode: the computer uses the rack and position number of a sample on the analyzer for identification. When the system is in position mode, do not use bar code labels on patient samples.
Page 159 ADVIA 2120/2120i Operator’s Guide Working with the Software 2. If the selected printer is directed to a file, you must enter the file name and path in the File field. 3. Select the Report Format options you want: Final Patient Report: The system automatically prints/transmits patient results in the Current Complete status using the report.par format.
Page 160 Working with the Software ADVIA 2120/2120i Operator’s Guide 2. Enter the code that corresponds to the Type Table you want, then select Enter. The default value is WB (whole blood). Closing the Window Select Exit. The Routine Parameters window closes and the Tools list displays.
Page 161 ADVIA 2120/2120i Operator’s Guide Working with the Software MOV. AVG. Close Generates a cumulative point for each test, using those data points available in the daily file with a date earlier than date in the Up to field. You can define the default date using Customer Parameters in Tools Modify.
Page 162 Working with the Software ADVIA 2120/2120i Operator’s Guide Stop Stops the selected procedures at the end of the current procedure, and cancels any additional selections. Clear Cancels the procedures selected in the End Of Day window. Save Saves the selections made in the End Of Day window. The selected procedures remain selected until you change them or cancel them using the Clear button.
Page 163 ADVIA 2120/2120i Operator’s Guide Working with the Software Consolidating the Quality Control (QC) NOTE: You must perform a QC Close Out before changing the Control lot. The QC Close Out function performs the calculations on daily entries for cumulative Quality Control. The cumulative calculation is performed using all QC results except those with a D disposition.
Page 164 Working with the Software ADVIA 2120/2120i Operator’s Guide Database The disks must be formatted, but they do not need to be labeled.The system will automatically label them during the backup procedure. NOTE: When several disks are required, the label for each disk must be the same.
Page 165 ADVIA 2120/2120i Operator’s Guide Working with the Software 3. After the disk is inserted, select OK in the message box. A message box is displayed to indicate the disk label and the date of the previous backup performed on this disk. The message also warns that the previous contents will be destroyed.
Page 166 Working with the Software ADVIA 2120/2120i Operator’s Guide Label: (none) Destroy contents ? The inserted disk has no name but contains some files. Selecting OK labels the disk and replace its contents with the current backup. Last backup date: MM/DD/YY...
Page 167 ADVIA 2120/2120i Operator’s Guide Working with the Software 6. Select OK in the dialog box. A message box displays asking you to confirm the purge. 7. To run the purge select OK. 8. To cancel the purge select Cancel. When the purge is started, the Stop button becomes available. If you select Cancel, the purge ends after the current sample is deleted.
Page 168 Working with the Software ADVIA 2120/2120i Operator’s Guide Modifying the Preselected Procedures To define the options that are automatically selected in the End of Day window the next time you open the window: 1. Delete all selected procedures select Clear.
Page 169 ADVIA 2120/2120i Operator’s Guide Working with the Software Menu Bar Profiles Allows access to the Profile Tables. A Profile Table is used to define the individual tests (defined in the Test Dictionary) that are included in a profile. Other Provides the following: Types Allows access to the Type Tables.
Page 170 Working with the Software ADVIA 2120/2120i Operator’s Guide After you select the List command, the work area displays the tests and ratios defined in the Test Dictionary. Tests and ratios display in alphabetical order, with up to 15 tests displayed at one time. Use the scroll bar to view and select additional tests.
Page 171 ADVIA 2120/2120i Operator’s Guide Working with the Software 3. Type in a code to identify the profile you want to create, then press Enter or Tab. The cursor displays in the first of the 70 fields available for entering the test codes.
Page 172 Working with the Software ADVIA 2120/2120i Operator’s Guide • Select the List command on the menu bar. Test codes defined in the Test Dictionary display in alphabetical order at the right-hand side of the test entry area. Use the scroll bar to view and select additional test codes.
Page 173 ADVIA 2120/2120i Operator’s Guide Working with the Software 7. To store this table in the dictionary, select OK. NOTE: If a test included in an order is not defined in the Test Order Table, this test is displayed in the Review / Edit tab after the ones defined in the Test Order Table.
Page 174 Working with the Software ADVIA 2120/2120i Operator’s Guide Type Table codes are composed of up to 3 alphanumeric characters. Table Dictionaries NOTE: To avoid deleting all prior test results, do not modify the Test Order Table while tests are being processed for a particular sample.
Page 175 ADVIA 2120/2120i Operator’s Guide Working with the Software 2. Enter the code of the control you want to view, then select Enter. Codes are case sensitive; a code typed in upper case letters is different from one typed in lowercase letters.
Page 176 Working with the Software ADVIA 2120/2120i Operator’s Guide 15. Type the target value for the control and select Enter. The cursor moves to the 2 SD box. 16. Type the standard deviation value and select Enter. 17. Optional: If you are defining control 0000(MOVAVG), enter the Min and Max values, selecting Enter after each.
Page 177 ADVIA 2120/2120i Operator’s Guide Working with the Software Editing a Control NOTE: To edit a control, the system must be in Tools Modify. 1. If the cursor is not in the Code box of the Control area, select the box to insert the cursor.
Page 178 Working with the Software ADVIA 2120/2120i Operator’s Guide 7. When you are finished editing the values for that test, select OK. The cursor displays in the Code box. • Enter the code for another test if you want to continue editing the same control.
Page 179 ADVIA 2120/2120i Operator’s Guide Working with the Software Menu Bar Doctors Selects the Doctors command to add, change, or delete entries in the Doctor Dictionary. Locations Selects the Locations command to add, change, or delete entries in the Location Dictionary.
Page 180 Working with the Software ADVIA 2120/2120i Operator’s Guide Doctor and Location Codes These codes can contain up to 6 alphanumeric characters and are always stored in capital letters in the dictionary. Comment Codes These codes can contain up to 5 alphanumeric characters. A code written with capital letters differs from a code written with lower case letters.
Page 181 ADVIA 2120/2120i Operator’s Guide Working with the Software Deleting an Entry from the Text Dictionaries 1. At the Text Dictionaries menu bar, select the command corresponding to the dictionary you want: • Doctors • Locations • Comments The corresponding dialog box opens. The cursor displays in the Code field.
Page 182 Working with the Software ADVIA 2120/2120i Operator’s Guide Test Dictionaries Use the Test Dictionary window to define the characteristics and ranges for each test processed by the system. Ratios are considered tests and must be defined in the dictionary as well as the tests included in ratios.
Page 183 ADVIA 2120/2120i Operator’s Guide Working with the Software Exit Closes the Test Dictionary window and returns to the Tools list. Next Displays the next test in the dictionary. Previous Displays the previous test in the dictionary. Tools Contains the following two items:...
Page 184 Working with the Software ADVIA 2120/2120i Operator’s Guide Work Area Buttons Update, Delete, OK, Cancel. Code Mandatory entry. Identifies the test within the dictionary and elsewhere (for example, Review/ Edit tab, Order Entry tab). NOTE: A code written with capital letters is different from the same code written with lower case letters.
Page 185 ADVIA 2120/2120i Operator’s Guide Working with the Software Active Optional entry. Improves the system performance by reducing the time required to read test characteristics. Since the number of tests that can be active is limited to 60, select this check box for the most frequently requested tests.
Page 186 Working with the Software ADVIA 2120/2120i Operator’s Guide CV% Max (P) Optional entry. Indicates the maximum value for the test’s coefficient of variation (CV%) that is defined by laboratory staff to apply limit comparison to the "Precision" control. When this value is entered on the system and the CV of a test result exceeds the value, the corresponding test results display on a color-coded background in the Quality Control window.
Page 187 ADVIA 2120/2120i Operator’s Guide Working with the Software Constants • 5 characters maximum including the decimal point • Up to 4 constants can be used in one formula NOTE: Do not create a formula containing only constants. Mathematical functions •...
Page 188 CV max%(C), Manual Dil. not applied, No. dec, Sex, Age Min, Age Max, Low/High, and Active fields, and the AutoRequest check box. NOTE: Dilution Ratio 1 is not for use with the ADVIA 2120/2120i Hematology System. 5. Define an image test without a reduced code.
Page 189 ADVIA 2120/2120i Operator’s Guide Working with the Software Platelet Results PLT, MPV, PDW, PCT Reticulocyte Results % RETIC, # RETIC, MCVr, CHCMr, CHr Morphology Results (user definable) WBC: Left Shift, Atypical Lymph, Blasts, Immature Granulocytes, Myeloperoxidase Deficiency, NRBC RBC: ANISO, MICRO, MACRO, HC VAR, HYPO, HYPER, RBC Fragments,...
Page 190 Working with the Software ADVIA 2120/2120i Operator’s Guide • Formula Field Entries are optional for the Reduced code, Name, D. Check %, CV max%(P), CV max%(C), Manual Dil. not applied, No. dec, Sex, Age Min, Age Max, and Low/High fields, and the AutoRequest check box.
Page 191 ADVIA 2120/2120i Operator’s Guide Working with the Software Test Name Test # dHGB RBC Channel CHCM RBC-vc RBC-ct RBC%DT %MACRO %MICRO %HYPER %HYPO RBC-FN RBCcoL RBCcoC CHDW #NEUTu #LYMPHu #MONOu #EOSu #BASOu #LUCu RBC-F RBC-G %M/%H PLT-n PLT-X PLT-Y 10708774 Rev.04...
Page 192 Working with the Software ADVIA 2120/2120i Operator’s Guide Test Name Test # PLT ID MPV ID PDW ID PLT-ct #L-PLT PCDW PMDW Perox Channel %NEUT %LYMPH %EOS %LUC %MONO #NEUT #LYMPH PX-d/D #EOS #LUC #MONO MPXI %NRBC #NRBC %HPX %MNu...
Page 193 ADVIA 2120/2120i Operator’s Guide Working with the Software Test Name Test # PX%DT PX-vc PX-tot PX-acq PX unIDed Clusters PX-Val LYMmod PX%Sat PX-FN NEUT-X NEUT-Y %Annormal cells WBCP WBCu %NEUTu %LYMPHu %MONOu %EOSu Baso Channel WBCB #BASO %BASO %PMN B%Sat...
Page 194 Working with the Software ADVIA 2120/2120i Operator’s Guide Test Name Test # %BASOu %LUCu %Blasts B-FN B%NO PMNrat Morphology ATYP BLASTS NRBC LPLT PLTCLM ANISO MICRO MACRO HC-VAR HYPO HYPER RBCF RBCG Retic Channel %RETIC #RETIC RTC-ct %L-RTC %M-RTC %H-RTC #L-RTC 10708774 Rev.04...
Page 195 ADVIA 2120/2120i Operator’s Guide Working with the Software Test Name Test # #M-RTC #H-RTC MCVr CHCMr RDWr HDWr CHDWr %H-CHr %HIPEr %HIPOr %L-CHr %MACr %MICr MCVg CHCMg RDWg HDWg CHDWg %H-CHg %HYPEg %HYPOg %L-CHg %MACg %MICg MCVm CHCMm RDWm HDWm...
Page 196 Working with the Software ADVIA 2120/2120i Operator’s Guide Test Name Test # %HYPOm %L-CHm %MACm %MICm %RBCm #RBCm RTCRBC RTCacq RTCana RTCg RTCabs RTC%NO RTC-FN RTC%DT RTC-vc IRF-H IRF-MH dMCV dCHCM dHDW dCHDW dRDW SLOPEm RTC-X RTC-Y PX Valley Count...
Page 197 ADVIA 2120/2120i Operator’s Guide Working with the Software Host Number Mandatory entry if the system is connected to a Host Computer or if the Active box is checked. Indicates the test number on the Host Computer. The Host number to enter depends on the system/Host communication protocol used.
Page 198 Working with the Software ADVIA 2120/2120i Operator’s Guide • Neperian (natural) logarithm (L), exponential (E), square root (S). Only one of these can be used per formula. This type of function must be placed at the beginning of the formula, and the function applies to the entire formula (no parentheses () needed).
Page 199 ADVIA 2120/2120i Operator’s Guide Working with the Software D. Check % Optional entry. Indicates the maximum percentage of acceptable deviation between the current result and the previous one. Any value exceeding this percentage triggers a Delta Check alarm. Depending on the alarm type defined in the Alarm Dictionary, this alarm can generate a rerun, or the display of the sample in the Review / Edit tab.
Page 200 Working with the Software ADVIA 2120/2120i Operator’s Guide Deleting Test Characteristics NOTE: You must validate all workorders in the database before you delete a test that is included in those workorders. Before deleting a test, do the following: • Check if this test exists in the Control Dictionary and the Table Dictionary.
Page 201 ADVIA 2120/2120i Operator’s Guide Working with the Software Opening the Ranges Area 1. Select the Ranges item to open the Ranges area. 2. Select the range type: Review, Report, Rerun, Panic, Dilution 1. The Characteristics and Ranges areas display on the screen. The cursor is positioned in the Code field of the Ranges area.
Page 202 Working with the Software ADVIA 2120/2120i Operator’s Guide 5. Type the minimum limit of the range in the Low field, then select Enter. 6. Type the maximum limit of the range in the High field, then select Enter. 7. Select the OK button to save the new range values.
Page 203 ADVIA 2120/2120i Operator’s Guide Working with the Software Mandatory entry if ranges are required. Specifies the lower limit value of the range. Any result less than this value is out-of-limit. High Mandatory entry if ranges are required. Specifies the higher limit value of the range. A result higher than this value is out-of-limit.
Page 204 Working with the Software ADVIA 2120/2120i Operator’s Guide Closing the Window To close the Alarm Dictionary window and open the Tools list select Exit at the menu bar. Work Area You can enter the following information in the Alarm Characteristics area: •...
Page 205 ADVIA 2120/2120i Operator’s Guide Working with the Software Indicates the action to be taken by the system for any result flagged with this alarm. Select one of the option buttons. If no option is selected in this field, the alarm is considered as an indicator;...
Page 206 Working with the Software ADVIA 2120/2120i Operator’s Guide 2. Enter the characteristics applicable to this alarm: Description and Type. 3. Select OK. The information is entered in the dictionary and the window is cleared and ready for your next entry.
Page 207: Port Configuration
ADVIA 2120/2120i Operator’s Guide Working with the Software Delete (C) The result is automatically dispositioned for deletion in the Review / edit tab. This alarm has priority over any other disposition taken by the system on the result. Changing the Characteristics of an Alarm 1.
Page 208 Working with the Software ADVIA 2120/2120i Operator’s Guide • The name of the software program resident on the computer that controls the communication port. Use the Port Configuration window in Tools View or Tools Modify as follows: • Tools View: View parameters •...
Page 209 ADVIA 2120/2120i Operator’s Guide Working with the Software Port Defines the communication port (Com1, Com2, and so on) used to connect the device. Setting Used to define the transmission characteristics (Baud, Parity, Number of databits, Number of stopbits), or used to display the NETWORK indication when the computer is connected to the device via a network.
Page 210 Working with the Software ADVIA 2120/2120i Operator’s Guide NOTE: Instrument connections (test requests and result transmissions) must be set upon lines Inst1, Inst2, and Inst3 of the Port Configuration window. • Serial or Network options Used to define whether the connection is asynchronous or via a network.
Page 211 ADVIA 2120/2120i Operator’s Guide Working with the Software Program Area • Name Mandatory entry. ○ The name of the program file that controls the communication ○ port. Enter in up to 8 alphanumeric characters, then select Tab. ○ • Maximum message size Mandatory entry.
Page 212 Working with the Software ADVIA 2120/2120i Operator’s Guide Deleting a Device 1. Open the Port Configuration window in Tools Modify. 2. Select the device you want to delete, then the Delete command at the Settings menu. A dialog box displays to indicate that all the device characteristics will erase.
Page 213 ADVIA 2120/2120i Operator’s Guide Working with the Software Allows access to the keywords that can be inserted in the formatting file. This command is available only when a file is loaded. File Management Format Keywords Patient keywords @PATNUM/14 Patient identification number...
Page 214 Working with the Software ADVIA 2120/2120i Operator’s Guide @F/1 Analytical flag associated with the result @N/1 Analyzer number Sample keywords @IDEE/14 Sample identification number (without leading spaces) @IDEZ/14 Sample identification number (with leading zeros) @COLDT/18 Collection date and time processed...
Page 215 ADVIA 2120/2120i Operator’s Guide Working with the Software @CPCOM/5 Patient coded comment @TPCOM/30 Patient comment text Indicates start of Result area. < Indicates the end of the information in the header that displays on each page of a multiple page report Result keywords @LOW./5...
Page 216 Working with the Software ADVIA 2120/2120i Operator’s Guide Patient Results Report Format Keywords Test keywords The test codes defined in the Test Dictionary with an @ character added in front. Patient keywords @PATNUM/14 Patient identification number @PATNAM/33 Patient name @SSEX/1...
Page 217 ADVIA 2120/2120i Operator’s Guide Working with the Software @SYSDT/18 System date and time @CLOC/6 Location identification code @TLOC/30 Location text or name @CDOC/6 Doctor identification code @TDOC/30 Doctor text or name @COCOM/5 Order coded comment @TOCOM/30 Order comment text @STYPE/3...
Page 218 Working with the Software ADVIA 2120/2120i Operator’s Guide @SYSDT/18 System date and time @ASPDT/18 Aspiration date and time @STYPE/3 Sample Type Creating a New Format File 1. Select New from the File menu. A dialog box displays allowing you to enter the name of the file management report to create.
Page 219 ADVIA 2120/2120i Operator’s Guide Working with the Software Entering keywords Keywords begin with the @ character. 1. Place the cursor at the position where the keyword should begin. 2. Select the option you want from the Key menu. • The Test item lists the test codes defined in the Test Dictionary.
Page 220 Working with the Software ADVIA 2120/2120i Operator’s Guide Then, while holding down the left mouse button, drag the cursor to the end of the text you want to select (several rows of text can be highlighted at one time). The selected text is highlighted and can now be copied and pasted, moved, or deleted.
Page 221 ADVIA 2120/2120i Operator’s Guide Working with the Software Deleting a Part of a Format File 1. Select the text you want to delete. 2. Select Cut from the Edit menu. The selected text is removed. Defining File Management List Formats You can define several list formats.
Page 222 Working with the Software ADVIA 2120/2120i Operator’s Guide 2. On the next lines, enter text and select the Key menu bar item to insert the keywords related to the necessary data. Only the following keywords are available in this area:...
Page 223 ADVIA 2120/2120i Operator’s Guide Working with the Software Resulting display or printout: PENDING 08/10/92 10:48 AM FILE LIST IDEE PAT#/NAME TEST 233 EVANS, R RBC HYPER ANISO RBC 237 ROGERS, G CHCM MCHC HGB MICRO Defining the Patient Result Report Format The following format files are used to define patient result reports: •...
Page 224 Working with the Software ADVIA 2120/2120i Operator’s Guide Test Result Layout 1. This area begins with the * character. On this line you can insert the following keywords (separated by a space): RESCOM, CRCOM, TRCOM, DISP, FLAG, NOT_REQ_TEST, PENDING_TEST. Refer to the Patient Result Report Format Keywords.
Page 225 ADVIA 2120/2120i Operator’s Guide Working with the Software • If you have inserted the @LOW and @HIGH keywords in place of the limits, the Report ranges defined for the patient age and sex in the Test dictionary will be printed in the result report.
Page 226 Working with the Software ADVIA 2120/2120i Operator’s Guide Defining the Control Result Report Format The control result report format file is QCREPORT.PAR. 1. Open a file as described in Opening a Format File. 2. Edit the file as described in the following topics: •...
Page 227 ADVIA 2120/2120i Operator’s Guide Working with the Software Header Layout 1. Insert the keywords related to control sample information in the header. To avoid generating an error, do not reuse a keyword in the header. Refer to the Control Result Report Format Keywords.
Page 228: Custom Parameters
Working with the Software ADVIA 2120/2120i Operator’s Guide Insert the image keyword before the 32nd column. The @ character marks the upper left corner of the image on the report. No other information must be set on the same line. If the image keyword is improperly set, the image will be centered in the document.
Page 229 ADVIA 2120/2120i Operator’s Guide Working with the Software The Customer Parameters window can be used in Tools View or Tools Modify as follows: • Tools View - To display parameters and their values • Tools Modify - To display parameters and modify their values File Menu •...
Page 230: Image Configuration
Working with the Software ADVIA 2120/2120i Operator’s Guide 3. Move the cursor onto the text box. 4. Use the Backspace or Del keys to erase the current value, then enter in the new value. Acceptable ranges and values are provided within brackets in the description of the selected parameter.
Page 231 ADVIA 2120/2120i Operator’s Guide Working with the Software Save Used to save the currently displayed configuration. The name of the configuration must not exceed 20 characters. Save as Used to duplicate the currently displayed configuration. The name of the configuration must not exceed 20 characters.
Page 232 Working with the Software ADVIA 2120/2120i Operator’s Guide • A subportion corresponds to a half size elementary bitmap. • The zoom function supported by the Review / Edit tab is allowed only on 7 of the 9 portions. The image printed by the Result Report function may be one of three types:...
Page 233: Screen Configuration
ADVIA 2120/2120i Operator’s Guide Working with the Software 3. To select an elementary bitmap by its name select the Image list box. 4. Double-select the Image list box to select and insert an elementary bitmap in the currently selected target portion of the image configuration area (equivalent to Insert on the Edit menu).
Page 234 Working with the Software ADVIA 2120/2120i Operator’s Guide Field Keyword Doctor Text @TDOC Sample Comment Code @COCOM Sample Comment Text @TOCOM Patient Comment Code @CPCOM Patient Comment Text @TPCOM Status @URGENT Dilution Dilution Sample Type @STYPE S.Blk @OPT Date of Birth...
Page 235 ADVIA 2120/2120i Operator’s Guide Working with the Software 3. Hold down the mouse button and move the selected field to the place where you want the selected field to be displayed. 4. Release the mouse button. When you select anywhere outside of the selected field, the field name is restored.
Page 236 Working with the Software ADVIA 2120/2120i Operator’s Guide 5. Select the selected field, then move the field to an appropriate location. 6. Select anywhere outside of the selected field. Canceling and Edit To cancel the previous action select the Undo command from the Edit menu.
Page 237 ADVIA 2120/2120i Operator’s Guide Working with the Software • Table dictionaries • Alarm dictionary • Text dictionaries You can select more than one check box. Selected printer indicates the printer on which printing is performed. If this printer is no longer available, the message "Not available!" displays instead of the printer name.
Page 238 Working with the Software ADVIA 2120/2120i Operator’s Guide Title Bar The title bar indicates "Spy View - file name" and the format of the open file (text or hexadecimal format). Menu Bar File Menu including the following items: Open Opens a dialog box used to select the file to be viewed.
Page 239 ADVIA 2120/2120i Operator’s Guide Working with the Software Command Buttons The line Selected printer in the command button area indicates the printer on which the selected printouts are performed. If the printer is no longer available, the message "Not available!" displays instead of the printer name.
Page 240 Working with the Software ADVIA 2120/2120i Operator’s Guide 3. To accept the deletion select OK. A message displays and asks you to confirm the deletion of the file. To cancel the operation and close the dialog box select Cancel. Renaming a .SPY File 1.
Page 241: Other Utilities
ADVIA 2120/2120i Operator’s Guide Working with the Software 3. In the text box, enter the character string you are searching for. The Match Case check box is available when searching for (a) character(s) in text format; the check box allows you to search for the necessary character(s) exactly as you have typed them (upper/lower cases).
Page 242 Working with the Software ADVIA 2120/2120i Operator’s Guide Initialize Menu This menu contains the following commands: • Patient & Sample Database Erases the database contents and all patient and sample information stored on the computer. When you select this command, the message "Patient &...
Page 243 NOTE: Restoring the dictionaries using files from another system can create inconsistencies between the Data Manager and the rest of the ADVIA 2120/2120i software. These inconsistencies will cause the system to malfunction. To avoid this, whenever you restore dictionaries using files from another system, also use the Backup / Restore tab on the Utilities menu to restore the settings from the same system.
Page 244 Working with the Software ADVIA 2120/2120i Operator’s Guide Extract Customized Parameter Files Allows you to extract copies of .INI, .PAR, and .LST files from the QC Dictionary backup file created during the End of Day procedure (dictqc.zip). These files may be useful in customizing or troubleshooting your system.
Page 245 ADVIA 2120/2120i Operator’s Guide Working with the Software View Menu This menu contains the following commands: • Toolbar Displays or hides the button bar. • Status bar Displays or hides the status bar. System Setup Tab NOTE: If Autoslide is installed the tab name is Analyzer Setup.
Page 246 Working with the Software ADVIA 2120/2120i Operator’s Guide Database Conditions This window determines the alarm/stop criteria for the percentage of disk space that has already been used. Use the Analyzer Status tab to check the current percentage of disk space used.
Page 247 ADVIA 2120/2120i Operator’s Guide Working with the Software Operating in rack and position mode When operating the Autosampler in rack and position mode, the system automatically assigns Rack = 0 and Tube = 99 to any sample for which the Autosampler cannot read the barcode.
Page 248 Working with the Software ADVIA 2120/2120i Operator’s Guide 2. For each sample/system flag: a. In the Alarm Criteria field, type the number of consecutive occurrences (0 to 100) at which an alarm message will display. Enter 0 to disable the alarm feature.
Page 249: Automation Screen
Responds to add queue requests by starting the AutoSampler. Status Line Automation Icon An Automation icon on the top-right-hand side of the ADVIA 2120/2120i status line may display the following colors: Green The AutoSampler will start in response to a request from the LAS.
Page 250 On the Startup screen there is a new LAS Online checkbox. If the checkbox is selected, the LAS is online and available for LAS queries. If the checkbox is not selected, the LAS can still query the ADVIA 2120/2120i instrument, but the instrument will not start the AutoSampler in response to an LAS request.
Page 251 ADVIA 2120/2120i Operator’s Guide Working with the Software • Primer samples • Control samples Patient samples NOTE: The Data Manager records only one aspiration for each patient sample. In the default setting, the Data Manager retains the first result for a sample and ignores additional results.
Page 252 Working with the Software ADVIA 2120/2120i Operator’s Guide Control samples To set the number of aspirations of each control sample run on the Autosampler: 1. Position the cursor in the Control text box and enter a number from 1 to 20.
Page 253 ADVIA 2120/2120i Operator’s Guide Working with the Software Auto Cycles Overview Use the Auto Cycles Setup window to schedule automatic analyzer cycles: • Auto Wash • Auto Rinse • Auto Standby • Auto Startup • Autoslide The system must be in the correct operating mode for an automatic cycle to occur.
Page 254 Working with the Software ADVIA 2120/2120i Operator’s Guide 2. Press the Tab key to move the cursor to the Minute field and enter the minute you want the wash cycle to begin. You can type the minute or use the arrow keys to increase or decrease the number.
Page 255 ADVIA 2120/2120i Operator’s Guide Working with the Software Auto Standby The system automatically switches into Standby mode whenever the analyzer is idle for a specified period of time. To set idle time before standby 1. In the Idle Time Before Standby field, enter the number of minutes the analyzer can remain idle before the analyzer automatically switches into Standby mode.
Page 256 Working with the Software ADVIA 2120/2120i Operator’s Guide Autoslide On systems equipped with an ADVIA Autoslide, you can set the autoslide idle time before the autoslide module performs a prime cycle or ejects the current rack. To set the Autoslide autocycles: 1.
Page 257 ADVIA 2120/2120i Operator’s Guide Working with the Software The Shortcut Label column lists the name that will display on the shortcut button for each selected window or tab. You can rename the shortcut button. The name must contain 17 or fewer characters.
Page 258 Working with the Software ADVIA 2120/2120i Operator’s Guide To use your own severity-level trigger values: 1. In the Customized Flags column, select the severity-level box (+, ++,+++) you want to change. 2. Enter the new value. 3. Repeat steps 1 and 2 for each value you want to change.
Page 259: Sample Identification
To print the Morphology Flagging Configuration Report, select Print Report. Report NRBC Results The ADVIA 2120/2120i reports a %NRBC and #NRBC result if the presence of nucleated red blood cells are detecting in the sample. The WBC result and differential are corrected for NRBCs.
Page 260 Working with the Software ADVIA 2120/2120i Operator’s Guide No Acquisition for Complete or Absent Workorders When this option is selected, any sample with no workorder or with no pending tests automatically skips aspiration by the analyzer and goes directly to the Autoslide for aspiration and slide production.
Page 261 . (period) as primers. Security Overview Use the Security window to display a list of all authorized ADVIA 2120/2120i system users and user codes. You can: • Add a new user •...
Page 262 Working with the Software ADVIA 2120/2120i Operator’s Guide Editing a User Edit an existing user name, user code, or password: 1. Select the user you want to edit by selecting the user name in the List of Users. 2. Select Edit User.
Page 263: System Options
The system name can be up to 10 alphanumeric characters long. 2. Select Save. NOTE: The change will not take effect in the Run Screen header until you restart the ADVIA 2120/2120i software. Autosampler On Select this option when the Autosampler is a part of your system configuration 1.
Page 264 Working with the Software ADVIA 2120/2120i Operator’s Guide CN Free HGB This option is normally selected to use the CN Free HGB reagent. 1. Unselect if you are using the HGB reagent 2. Select Save Ensure you have the correct hardware to use the HGB reagent.
Page 265 ADVIA 2120/2120i Operator’s Guide Working with the Software NOTE: Use Windows to add a new printer. You can also purge the buffer of the printer listed in the box. This cancels any pending print orders on that printer. Purge the Printer Buffer To purge the printer buffer 1.
Page 266 Working with the Software ADVIA 2120/2120i Operator’s Guide 2. Enter the path and directory in which you want to save the raw data. The default is C:\raw_data. 3. Select Save. NOTE: Selecting Save creates the raw data folder. If you enter a subfolder name make sure the parent folder exists before selecting Save.
Page 267 ADVIA 2120/2120i Operator’s Guide Working with the Software Each of the following lines contains the research parameters for a single sample. The first 3 columns contain the sample ID, the sequence number, and the aspiration date and time for sample identification. The next...
Page 268 Working with the Software ADVIA 2120/2120i Operator’s Guide Cluster Description Parameter Area Area of located cluster. Weight Calculated final weight of cluster used for final identification of all cytogram positions File Export Set the system to save result data to a file: 1.
Page 269 ADVIA 2120/2120i Operator’s Guide Working with the Software Run Screen Options Auto Print Run Screen The Run Screen has 3 auto print options: Print All Select this option to set the system to automatically print the Run Screen for every sample.
Page 270 Working with the Software ADVIA 2120/2120i Operator’s Guide NOTE: If you select a Morphology Flag at the + or++ levels, the system automatically prints the Run Screen for samples that trigger the higherlevels as well. Example: When you enter a check mark in the + column, the Run Screen for samples with ++ and +++ for that flag also print.
Page 271 ADVIA 2120/2120i Operator’s Guide Working with the Software 5. Select OK in the Save Unit Set Settings box if you are sure you want to save the new unit set. Select Cancel to close the Save Unit Set Settings box without saving the new unit set.
Page 272 Body Fluids Sample/System Flags (For use with ADVIA 2120/2120i systems only) A selected group of ADVIA 2120/2120i sample/system flags are used by the Body Fluid Method to identify samples that may have invalid TNC or RBC counts. The first table below lists the sample/system flags modified for the Body Fluid Method and their triggering conditions.
Page 273 ADVIA 2120/2120i Operator’s Guide Working with the Software Sample/System Flags Modified for the Body Fluid Method Sample/System Flag Triggering Condition WBC-CE TNC 100-2000 cells/µL AND WBC Comparison Error %Perox Noise > 50 and the ABS (WBCB-WBCP) ≥ 40% WBCB TNC ≤ 2000 cells/µL AND %Perox Noise ≤...
Page 274 Working with the Software ADVIA 2120/2120i Operator’s Guide Sample/System Flags Not Modified for the Body Fluid Method Sample/System Flag Triggering Conditions LAS-PL (PL) The laser light intensity in the RBC/Baso/Retic Laser Power Low channel is less than 150. PX-PL (PX)
Page 275 Principles of the Procedure (For use with ADVIA 2120/2120i systems only) Assay Principle The ADVIA 2120/2120i Body Fluid Method uses the Basophil/Lobularity and RBC/Plt channels to enumerate the TNC and RBC counts. The TNC count is derived from the Basophil/Lobularity channel. The...
Page 276 Working with the Software ADVIA 2120/2120i Operator’s Guide Using the the MOTS and MCTS 1. If the Standby indicator is lit, select Standby to place the system in Ready to Run mode. 2. Ensure that the system passed Startup. NOTE: Ensure the system is not in the Body Fluid mode before you select Refresh at the Startup screen.
Page 277 ADVIA 2120/2120i Operator’s Guide Working with the Software 9. When running multiple body fluid specimens, select the Body Fluid Rinse button at the bottom of the screen to run the Body Fluid Rinse cycle between samples to reduce the effects of carryover.
Page 278 ADVIA 2120/2120i Operator’s Guide NOTE: Windows in the Tools look and behave differently from the rest of the ADVIA 2120/2120i software. They contain a menu bar of their own and you must manually close each window before you open another window or tab.
Page 279 NOTE: Restoring the dictionaries using files from another system can create inconsistencies between the Data Manager and the rest of the ADVIA 2120/2120i software. These inconsistencies will cause the system to malfunction. To avoid this, whenever you restore dictionaries using files from another system, also use the Backup / Restore tab on the Utilities menu to restore the settings from the same system.
Page 280 Working with the Software ADVIA 2120/2120i Operator’s Guide Program backup Restores the programs using a backup made using the End of Day window. This command should be performed with the help of a Field Service Engineer. When you select this command, the message "Programs are going to be copied from backup diskette to hard disk!"...
Page 281 ADVIA 2120/2120i Operator’s Guide Working with the Software Status bar Displays or hides the status bar Data Manager Menu Sample Control Panel Tab The Sample Control Panel tab gives you an overview of the system’s operation and lets you open other tabs to perform tasks or get more detailed information.
Page 282 Working with the Software ADVIA 2120/2120i Operator’s Guide The default communication links are: Link to the host computer The indicator changes color only when the system processes a sample. As a result, if the link with the host is broken, the indicator does not turn red until the system processes the next sample.
Page 283 ADVIA 2120/2120i Operator’s Guide Working with the Software 2. To view the samples in the Review/Edit tab, select Rev/Edit at the bottom of the tab. 3. To view the samples in File Management, select File Mgt. You cannot view samples using either button if the count for the status you have selected is zero.
Page 284 Working with the Software ADVIA 2120/2120i Operator’s Guide Sample Status (Sample Control Panel) 10708774 Rev.04...
Page 285 ADVIA 2120/2120i Operator’s Guide Working with the Software Sample Status (Sample Control Panel) Pending (dark Samples with no test results, missing results, or that grey) are scheduled for a rerun. Samples counted in the Rerun status are included in this number.
Page 286 Working with the Software ADVIA 2120/2120i Operator’s Guide Viewing Color-Coded Control Results Viewing Results for All Control Samples If you do not select any control samples in the Sample List, the color-coded Test Table Grid represents the results for all control samples. The background color for each test represents the largest deviation (positive or negative) among the samples.
Page 287 ADVIA 2120/2120i Operator’s Guide Working with the Software Releasing Samples from the Hold Status 1. Select a control sample or batch of samples in the Hold status by selecting a control sample or batch of samples in the Sample List.
Page 288 Working with the Software ADVIA 2120/2120i Operator’s Guide 7. To close the dialog box without applying a disposition select Cancel. Dispositions you apply using the Sample Control Panel have priority over all dispositions automatically proposed by the system. They also have priority over dispositions you apply using the Review/Edit tab except for Edit and Exchange.
Page 289 ADVIA 2120/2120i Operator’s Guide Working with the Software 2. In the Pat# box, type the patient number, then select Enter or select 3. If there is more than one workorder for this patient, they are listed. 4. To make a new workorder for this patient select Create.
Page 290 Working with the Software ADVIA 2120/2120i Operator’s Guide 5. Select tests. 6. To confirm the entries select OK. 7. The entries are cleared, and the SID# increments by one. 8. If necessary, you can change the proposed SID#. With Auto R&P# To automatically increment the sample rack and position number (R&P#)
Page 291 ADVIA 2120/2120i Operator’s Guide Working with the Software 5. Optional: Enter sample and patient information. Use the Tab key to move from field to field. 6. Select tests. 7. To confirm the entries select OK. The entries are cleared. The SID#, and the rack and position number increment by one.
Page 292 Working with the Software ADVIA 2120/2120i Operator’s Guide 4. To move the cursor to the next field select the Tab key. 5. Enter the dilution factor (1–5000) in the Dilution Factor box and select the Tab key. The tests you selected for the Dil.Sample disposition display in the Selected tests box.
Page 293 ADVIA 2120/2120i Operator’s Guide Working with the Software Rerun Selecting Rerun from the Disposition menu requests a repeat of the test with no dilution. • An “R” disposition code displays in the D column. • The first result for the test moves to the PREV.RUN column.
Page 294 Working with the Software ADVIA 2120/2120i Operator’s Guide Insert (Optional) You can set the software to display tests that were not selected in the workorder, but were run by the analyzer as part of the default profile. Selecting Insert from the Disposition menu changes these non-requested results into inserted results that are reported to the host computer.
Page 295 ADVIA 2120/2120i Operator’s Guide Working with the Software • You can update the manual dilution factor only while the sample is in Pending status. • You cannot select ratios for diluted samples. • Changes to a patient’s age and/or sex are not applied retroactively for the range checking of existing results, but they are used for any newly processed results.
Page 296 Working with the Software ADVIA 2120/2120i Operator’s Guide 6. To restore the original workorder entries select Reset. Deleting To delete a workorder: Guidelines for deleting workorders 1. Find the workorder: • by sample identification number (SID#) • by patient number (pat#) •...
Page 297 ADVIA 2120/2120i Operator’s Guide Working with the Software 3. Select the correct patient, then select Update. If there is more than one workorder for the patient, they display in a list. 4. Select the workorder you want, then select Update.
Page 298 Working with the Software ADVIA 2120/2120i Operator’s Guide Managing Baseforms Creating Create a workorder baseform: 1. At the Parameter menu, select Baseform definition, then select New on the submenu. 2. Enter sample information and select tests. NOTE: Leave the Asp/Time/Date field empty when entering the sample information.
Page 299 ADVIA 2120/2120i Operator’s Guide Working with the Software Deleting Delete a workorder baseform: 1. At the Parameter menu, select Baseform definition, then select Open at the submenu. 2. Type or select the filename for the necessary baseform, then select OK.
Page 300 Working with the Software ADVIA 2120/2120i Operator’s Guide • If the existing sample is in the All Complete status, the All Complete sample is not modified and a new workorder is created in the Pending file. • If the workorder update corresponds to an Unmatched sample, the matching occurs automatically (regardless of the update indicator value).
Page 301 ADVIA 2120/2120i Operator’s Guide Working with the Software 6. To confirm the workorder and exit the Diluted Sample - Order window, select OK. 7. Select Next to accept the workorder and order another dilution. You may order up to 10 for each original sample. Each workorder must have a different sample ID number.
Page 302 Working with the Software ADVIA 2120/2120i Operator’s Guide • The records with the earliest date and time of aspiration display first. • If no sample result is in the status file you select, an “End of file” message displays. Select Enter, or select OK to close the message.
Page 303 ADVIA 2120/2120i Operator’s Guide Working with the Software 5. The sample status displays on the title bar. You can use the Next and Previous arrow buttons on the Review/Edit tab toolbar to view other samples in the same status file.
Page 304 Working with the Software ADVIA 2120/2120i Operator’s Guide By Patient Name To view sample results by Patient Name: 1. At the Review/Edit tab Access menu, select PatName. 2. In the PatName dialog box, enter the name of the patient for the sample you want to view and select OK.
Page 305 ADVIA 2120/2120i Operator’s Guide Working with the Software 4. To move among the samples in the selected status that were aspirated later than the date and time you entered use the Next and Previous arrow buttons on the Review/Edit tab toolbar.
Page 306: Adding Tests
Working with the Software ADVIA 2120/2120i Operator’s Guide If you edit a test included in the 100% Table defined in the Table Dictionary, the system checks to make sure that the total for the tests equals 100%.If the total does not equal 100%, a message displays to inform you of the error.
Page 307 ADVIA 2120/2120i Operator’s Guide Working with the Software 5. The Result Entry dialog box opens automatically for you to add the appropriate result. • If you are only adding one test, enter the result and select End. • If you are adding multiple tests, enter the result for the first additional test and select Next.
Page 308 Working with the Software ADVIA 2120/2120i Operator’s Guide 6. At The Review/Edit tab toolbar, select Validate to validate the results. Once you validate the results, the record automatically moves to the Current Complete file and is transmitted to the host computer and/or printer.
Page 309 ADVIA 2120/2120i Operator’s Guide Working with the Software 2. Select OK. A message displays asking if you want to match the results to the workorder with the sample ID number you entered. • To match the results with the workorder select OK.
Page 310 Working with the Software ADVIA 2120/2120i Operator’s Guide Ordering and Consolidating Sample Dilutions Creating Diluted Sample Workorders 1. View the original record for the sample you want to dilute. 2. At the Sample menu, select Global Disposition, and then select the Dil.Sample disposition.
Page 311 ADVIA 2120/2120i Operator’s Guide Working with the Software 2. Select Dil.Cons. The Diluted sample - Consolidation window opens. If necessary, use the scroll bars to see all the results. 3. Select the results you want by double-selecting them. • The number of results you select displays in the Kept box on the status bar.
Page 312 Working with the Software ADVIA 2120/2120i Operator’s Guide Printing Image Results 1. View the sample for which you want to print the images. • If the images are not visible when the sample record displays, select the Toggle Image button on the Review/Edit tab toolbar.
Page 313 ADVIA 2120/2120i Operator’s Guide Working with the Software Validating Results 1. Before running samples through the analyzer, select a validation mode from the Review/Edit tab Parameter menu. You can change the validation mode at any time, but the change only affects sample results received after the change.
Page 314 Working with the Software ADVIA 2120/2120i Operator’s Guide Each of these groups of information can contain two types of sample results: Control Control sample results compared to known values for the control material in order to establish analyzer accuracy and reliability Moving Average Patient sample results compared to each other in order to track drifts in analyzer performance over time.
Page 315 ADVIA 2120/2120i Operator’s Guide Working with the Software 10. If you are viewing the 3D bar graph or the scatter chart, select a sample line in the List box to highlight the bar or data point for that sample on the graph.
Page 316 Working with the Software ADVIA 2120/2120i Operator’s Guide Printing QC Reports There are two ways you can print using the QC tab: • Print screen • Print a report Printing: The Screen To print the information displayed in the QC tab, including the graph, select Print Screen from the File menu (QC tab).
Page 317 ADVIA 2120/2120i Operator’s Guide Working with the Software The system recognizes a control sample and generates an appropriate record of the results if the barcode label is in the format %YYYY. The system assigns the control sample record a sample ID number in the format C-YYYY-HHMMSS.
Page 318 Working with the Software ADVIA 2120/2120i Operator’s Guide NOTE: The system default settings require you to review all control results in the Review/Edit tab. To disable this requirement, use Customer Parameters in Tools Modify to set Review None mode for control samples= Y.
Page 319 ADVIA 2120/2120i Operator’s Guide Working with the Software • The default settings include flagged results in the QC calculations. If you want to set your system to exclude flagged results, contact your local technical support representative for assistance. If you have any questions or concerns regarding this document, contact your local technical support provider or distributor.
Page 320 Working with the Software ADVIA 2120/2120i Operator’s Guide 3. Select the test or all tests for the QC record. • To select a single test, double-select the data point in the table. • To select all tests, double-select the time and date of the QC Close Out.
Page 321 ADVIA 2120/2120i Operator’s Guide Working with the Software Cumulative reports include daily data if the control has not been closed out. All Controls Daily Summary The daily summary report includes daily quality control information for all tests and all controls defined in the Control Dictionary.
Page 322 NOTE: When you open the Remote Quality Control tab, the Remote Quality Control fills the screen, covering the rest of the ADVIA 2120/2120i software. Resize the window to expose the menu bar, status lines, and tabs. Remote Quality Control can work as a stand-alone application.
Page 323 4. In the Path field, enter the system name you want to access and the path of the shared directory as follows: \\Remote_system_name\Directory_to_share\ Example • To be connected to the ADVIA 2120-2 system’s QC database stored in the directory C:\WST2 enter: \\ADVIA 2120-2\C\WST2\. • To view all the accessible directories select Browse. 10708774 Rev.04...
Page 324 Working with the Software ADVIA 2120/2120i Operator’s Guide 5. Select one then select OK. Your selection displays in the Access Path field. The system checks that a database exists in this directory.A message is displayed if the database cannot be accessed.
Page 325: Terminal Window
ADVIA 2120/2120i Operator’s Guide Working with the Software Printing the Quality Control for Several Systems When more than one system is selected in the File Access list, only the All Controls options are available. Select the Daily summary or the Cumulative summary and select OK.
Page 326 Opening the Playback window 1. Log on to the Windows operating system using a supervisor or field service password. 2. Log on to the ADVIA 2120/2120i software. 3. To display the Start menu, select Ctrl + Esc. CAUTION Do not open multiple Playback windows. Opening multiple Playback windows can cause the software to malfunction.
Page 327 Select from the following options: Playback Options • Species For use with the ADVIA 2120/2120i MultiSpecies software package: Select Stored to use the species selected when the sample was ○ originally run on the system. The Species Type field in the File Details area lists the original species.
Page 328 Working with the Software ADVIA 2120/2120i Operator’s Guide • Cal Factors Select Stored to use the original cal factors. ○ Select Current to use the current cal factors. ○ • Morphology Select Stored to use the original morphology flag trigger values.
Page 329 ADVIA 2120/2120i Operator’s Guide Working with the Software • Blend Function Select this feature to have the raw data from multiple aspirations combined into a single analysis.This feature can be used to obtain an analysis for a sample with a low cell count. The Sample ID and other header data is taken from the first sample.
Page 330 Working with the Software ADVIA 2120/2120i Operator’s Guide 10708774 Rev.04...
Page 331: Daily Routine
4 Daily Routine Emptying the Waste Container BIOHAZARD Use Hematek Stain Paks only. Other solutions may contain non-dissolved or particulate materials. BIOHAZARD All products or objects that come in contact with human or animal body fluids should be handled, before and after cleaning, as if capable of transmitting infectious diseases.
Page 332 Daily Routine ADVIA 2120/2120i Operator’s Guide 3. Disconnect the waste line and the vacuum line. a. Disconnect the lines by selecting the buttons on the quick-release connectors as you pull the lines straight up. Waste line Vacuum line Quick release buttons...
Page 333 ADVIA 2120/2120i Operator’s Guide Daily Routine Automatic Waste Removal CAUTION Ensure all cycles are complete before disconnecting the waste container. The waste container must be connected to the system when any system cycles are in progress, including the system startup cycle. If the waste container is not connected, fluid will back up into the UFC and damage the system.
Page 334 Daily Routine ADVIA 2120/2120i Operator’s Guide 4. At the waste removal assembly tray, turn the mode selector knob from NORMAL to EMPTY. Level-sensor switch connector Selector knob Discharge line 5. The waste in the container begins to empty. Completely emptying the waste container takes between 2 and 5 minutes.
Page 335 ADVIA 2120/2120i Operator’s Guide Daily Routine Waste Container Vacuum Line Pressure Control Selector Knob Fill Sensor On/Off Switch Emptying the Overflow Bottle NOTE: If liquid consistently accumulates in the overflow bottle, contact your local service provider for assistance. If you have any questions or concerns regarding this document, contact your local technical support provider or distributor 10708774 Rev.04...
Page 336 1. Use the Startup tab or the Reagent Log tab to check the supply of all reagents except the ADVIA 2120/2120i DEFOAMER. 2. Visually check the supply of ADVIA 2120/2120i DEFOAMER. 3. If you need to replace reagents, use the Reagent Log tab.
Page 337 ADVIA 2120/2120i Operator’s Guide Daily Routine Obtaining the Background Counts Run a background count cycle to obtain a BASO WBC background count, a platelet background count, and an HGB Trans. 1. At the Operations menu, select Startup. 2. Select Refresh.
Page 338: Running The Samples
Run controls in accordance with your laboratory protocol. • Run multilevel controls at the beginning of each shift. Siemens recommends using ADVIA TESTpoint Hematology Controls, ADVIA TESTpoint Reticulocyte Control (Low and High), or ADVIA TESTpoint 3-in-1 controls (Abnormal1, Normal Abnormal 2).
Page 339 ADVIA 2120/2120i Operator’s Guide Daily Routine • Optionally, you can run a retained whole blood sample periodically to monitor performance trends. • Evaluate all control results before reporting patient results. • If control results fail to meet the laboratory's established criteria for...
Page 340 Daily Routine ADVIA 2120/2120i Operator’s Guide 3. Scan the tube label or enter the sample information in the Manual Sample ID tab. CAUTION Make sure the correct sample ID displays on the status line before aspirating a sample using either the manual open-tube sampler or the manual closed-tube sampler.
Page 341 ADVIA 2120/2120i Operator’s Guide Daily Routine Viewing the Sample Run 1. At the Data Manager menu, select Sample Cont. Panel. 2. Use the database status area to select records by sample status. 3. To display a list of samples with a specific status, select the status box, then select File Mgt.
Page 342 Daily Routine ADVIA 2120/2120i Operator’s Guide 2. Select a disposition. None cancels any previous disposition. Rerun repeats test with no dilution using the same sample ID. The letter R displays in D box. Exchange swaps information in C.RES and PREV.RUN boxes. The letter X displays in D box.
Page 343 ADVIA 2120/2120i Operator’s Guide Daily Routine 2. Select System Wash, select 1 for the Number of Cycles, and then select Start. Performing the End of Day Procedure Resetting SIDs, and closing out the QC and Moving average statistics (Tools View) 1.
Page 344 To visually identify each disk, write a sequence number on the disk paper label only. Formatting a CD 1. Ensure the ADVIA 2120/2120i system interface is in Ready to Run status. 2. Insert a writable CD into the CD-ROM drive.
Page 345: Logging Off
ADVIA 2120/2120i Operator’s Guide Daily Routine Logging Off 1. At the Operations menu, select Log On / Off. 2. Select Log Off. 10708774 Rev.04...
Page 346 Daily Routine ADVIA 2120/2120i Operator’s Guide 10708774 Rev.04...
Page 347: Maintenance
5 Maintenance Schedule Maintain the operating efficiency of your analyzer by performing specific procedures according to the frequency listed below. Daily • Perform automated system washes. Perform the automated system wash procedure at the end of each shift or work period (a maximum of 8 hours). After the laboratory shift with the largest number of samples, run 3 system wash cycles;...
Page 348 Maintenance ADVIA 2120/2120i Operator’s Guide NOTE: In addition to these scheduled procedures, periodic inspections of the UFC pathways, vacuum shuttle, and reaction chambers are essential. If you find build up or dirt in any of the lines or chambers, clean those lines or chambers.
Page 349 ADVIA 2120/2120i Operator’s Guide Maintenance Inspecting the Perox-Cap Vent Hole for Buildup NOTE: For those systems running a software version prior to version 6.10.9. Overflow tube 1. Disconnect the overflow tube from the perox vent. 2. Remove the cap (2).
Page 350 Maintenance ADVIA 2120/2120i Operator’s Guide Time: 4 minutes Analyzer mode: Ready to Run NOTE: Clean 1 vent line or chamber at a time. 1. Remove the tubing from the overflow bottle that leads to the vent of the pathway or chamber to clean.
Page 351 ADVIA 2120/2120i Operator’s Guide Maintenance Flowcell Wash NOTE: For systems running software versions prior to 6.10.9.This function flushes a clogged flowcell and is available from the Hydraulic Functions tab. Wash the perox flowcell using EZ Wash. Wash the RBC/baso/retic flowcell, using a 25% solution of household bleach and water.
Page 352 Maintenance ADVIA 2120/2120i Operator’s Guide BIOHAZARD All products or objects that come in contact with human or animal body fluids should be handled, before and after cleaning, as if capable of transmitting infectious diseases. Wear facial protection, gloves, and protective clothing.
Page 353 ADVIA 2120/2120i Operator’s Guide Maintenance Cleaning the Centering Collar Clean the Autosampler Closed Tube Centering Collar (ACTS) bi-weekly or as needed. Clean the Manual Closed Tube Centering Collar (MCTS) monthly, or as needed. BIOHAZARD All products or objects that come in contact with human or animal body fluids should be handled, before and after cleaning, as if capable of transmitting infectious diseases.
Page 354 Maintenance ADVIA 2120/2120i Operator’s Guide Removing the Centering Collar from the Autosampler 1. Remove the sample line from the bottom of the needle base. Sample line Thumb screws Spring-loaded knob Centering collar CAUTION You must remove the sample line before the aspirator assembly is tilted forward.
Page 355 ADVIA 2120/2120i Operator’s Guide Maintenance Removing the Centering Collar from the Manual Closed-Tube Sampler 1. Pull the centering collar up. Nipple 2. Remove the tubing from the nipple. • Remove both tubing from both nipples. 3. Place the red needle cover over the needle.
Page 356 Maintenance ADVIA 2120/2120i Operator’s Guide 4. Using a stylet or thin wire, clean the 3 nipples and the center bore on the Autosampler centering collar; or, the nipple and center bore on the manual closed-tube sampler centering collar. 5. Attach a piece of 0.030-inch ID tubing to a syringe, then flush each port on the Autosampler collar or the waste port on the manual closed-tube sampler collar with water.
Page 357 ADVIA 2120/2120i Operator’s Guide Maintenance 8. On the Autosampler, reposition the Autosampler aspirator assembly. Ensure the assembly drops firmly in place over the guide pins, then reconnect the sample line to the base of the centering collar. CAUTION After repositioning the aspirator assembly, finger-tighten the thumb screws, being careful that they are not cross threaded.
Page 358 Maintenance ADVIA 2120/2120i Operator’s Guide 5. Select valves V1, V47, and V74 to open. Closed Valve Open Valve 6. Hold a beaker of sodium hypochlorite under the open-tube sample probe until 5 mL is aspirated. 7. Repeat step 6 using 5 mL of water.
Page 359 Starting the Automatic Hydraulic Pathway Wash The recommended frequency for these washes is weekly. 1. Allow 2 vials of ADVIA 2120/2120i RBC Flow Cell Wash and 5 vials of ADVIA 2120/2120i Aspiration Pathway Wash stand for 15-20 minutes to reach room temperature.
Page 360: Cleaning The Shear Valve
Maintenance ADVIA 2120/2120i Operator’s Guide 5. At the Utilities menu, select Hydraulic Functions. Then select clean UFC Vent Lines and Chambers. 6. Select All, then Start. 7. After completing the cleaning, place the tubing into a container with 100mL di-Water and repeat the above steps.
Page 361 ADVIA 2120/2120i Operator’s Guide Maintenance Disassembling the Shear Valve Faces NOTE: Place paper towels directly under the shear valve to prevent fluid from dripping into the analyzer. 1. Remove the knurled nut by turning counter clockwise, then remove the compression spring.
Page 362 Maintenance ADVIA 2120/2120i Operator’s Guide 3. Remove the front shear face by gently rotating the front face until the front shear face is loosened, then pull forward and remove. The rear shear face is stationary. CAUTION Avoid damaging the seal that secures the shear valve to the acrylic layer of the UFC.
Page 363 ADVIA 2120/2120i Operator’s Guide Maintenance Reassembling the Shear Valve CAUTION You can assemble the shear valve while the faces are still wet. Never use paper towels, gauze, or cotton swabs on the shear faces. These materials can leave fibers on the surface that can clog the precision grooves.
Page 364 Maintenance ADVIA 2120/2120i Operator’s Guide Inspecting the Syringe Plungers Inspect the tip of the plunger during a saline prime. as needed. The normal wear of the plunger tip (sheath 1 [1000 µL syringe] and sample 2 [50 µL syringe]) against the syringe barrel can cause small pieces to be detached.
Page 365 ADVIA 2120/2120i Operator’s Guide Maintenance Cleaning the Syringes and Plungers 1. Remove the syringe. a. Turn the thumb wheel counterclockwise until the plunger is lowered about 1.27 c ( inch). Thumb wheel Input fitting Output fitting b. Disconnect the input and output fittings from the syringe head.
Page 366 Maintenance ADVIA 2120/2120i Operator’s Guide e. Use a small hex wrench to pop the bushing out of the syringe. f. Remove the plunger from the syringe with both bushings still on the plunger stem. Do not remove the plastic bushings from the 50 µl plunger.
Page 367 ADVIA 2120/2120i Operator’s Guide Maintenance 6. Checking Analyzer Performance a. Check saline backgrounds b. Run a whole blood prime. c. Run controls. Replacing the Sampler Needles There are 2 needles in the analyzer. One needle is located in the Autosampler and the other needle is in the manual closed-tube sampler.
Page 368 Maintenance ADVIA 2120/2120i Operator’s Guide Figure 1: Locating the sampler needles and centering collars Autosampler Manual closed-tube sampler BIOHAZARD All products or objects that come in contact with human or animal body fluids should be handled, before and after cleaning, as if capable of transmitting infectious diseases.
Page 369 ADVIA 2120/2120i Operator’s Guide Maintenance 6. Install the replacement needle. a. Remove the clear plastic end of the needle cover. Needle cover, clear plastic end b. With the red needle cover in place, screw the needle clockwise into the needle base. Be careful not to cross-thread the needle.
Page 370 Maintenance ADVIA 2120/2120i Operator’s Guide 8. Carefully replace the collar over the needle. On the Autosampler centering collar, be sure to turn the spring loaded knob back to the knob’s original position. 9. On the Autosampler, reposition the aspirator assemble. Ensure that the assemble drops firmly in place over the guiding pins, then, reconnect the sample line to the base of the centering collar.
Page 371 ADVIA 2120/2120i Operator’s Guide Maintenance Perox sheath filter RBC/baso sheath filter 1. Carefully pull the filter toward you just enough to remove the filter from the mounting and gain access to the tubing. Place paper towels under the filter to catch any fluid that leaks out when you remove the filter.
Page 372 Maintenance ADVIA 2120/2120i Operator’s Guide 4. Disconnect the luer fitting from the connector at the output port. Do not disconnect the line leading from the luer fitting. 5. Discard the filter according to local environmental laws and regulations. 6. Hold the replacement sheath filter by the body and attach the luer fitting to the connector.
Page 373 ADVIA 2120/2120i Operator’s Guide Maintenance Replacing the 50 µl or 1000 µl Syringe Plungers Materials Required Hex wrench, small Sample syringe repair kit Household bleach Sheath syringe repair kit Time: 15 minutes Analyzer Mode: Off NOTE: The sheath syringe plungers will last 2-3 times longer that the sample syringe plungers.
Page 374 Maintenance ADVIA 2120/2120i Operator’s Guide BIOHAZARD All products or objects that come in contact with human or animal body fluids should be handled, before and after cleaning, as if capable of transmitting infectious diseases. Wear facial protection, gloves, and protective clothing.
Page 375 ADVIA 2120/2120i Operator’s Guide Maintenance 4. Slide the syringe barrel down toward the bottom of the carriage, then remove. CAUTION The 50 µl syringe plunger has a small plastic bushing inside the syringe. When replacing the plunger, never pull the plunger through the small bushing, because this action will cause damage to the plunger tip.
Page 376 Maintenance ADVIA 2120/2120i Operator’s Guide 7. Use the hex wrench to loosen the set screws on the metal bushing on the plunger stem. Remove and keep the bushing. Discard the plunger. Set screws Metal bushing Plunger Pin gauge 8. Inspect the syringe, if the inside surface is dirty, use a wash bottle with 25% solution of household bleach and water to clean the syringe.
Page 377 ADVIA 2120/2120i Operator’s Guide Maintenance d. Replace the metal bushing onto the plunger stem with the narrow collar of the bushing toward the plunger. Narrow collar of bushing Plunger e. Push the metal bushing up against the syringe, then tighten the set screws.
Page 378 Maintenance ADVIA 2120/2120i Operator’s Guide Cleaning the Air-Circulation Filter Time: 15 minutes 1. Remove the filter by sliding the filter to the right, out of its frame. Air circulation filter 2. Remove excess dust or lint from the filter by tapping the filter against a clean, hard surface or by vacuuming.
Page 379 ADVIA 2120/2120i Operator’s Guide Maintenance Time: 15 minutes Analyzer mode: Off 1. Turn off the analyzer. 2. Tilt the front cover down. 3. Thoroughly rinse the Autosampler aspirate assembly with deionized water. NOTE: Single aspirate assemblies have 1 needle, centering collar, and air cylinder.
Page 380 Maintenance ADVIA 2120/2120i Operator’s Guide 7. Allow the assembly to stand for 5 minutes, then wipe the assembly dry with paper towels and cotton swabs. Repeat if necessary. 8. Reposition the assembly. CAUTION After repositioning the aspirator assembly over the guide pins on either end, finger-tighten the thumb screws, being careful that they are not cross threaded.
Page 381: Troubleshooting
6 Troubleshooting If you are unable to correct a problem and require service assistance, contact your local technical support provider for assistance. If you have any questions or concerns regarding this document, contact your local technical support provider or distributor. Alignments and Adjustments Adjusting the Hgb Baseline Value •...
Page 382 Troubleshooting ADVIA 2120/2120i Operator’s Guide 5. As the analyzer is checking the baseline value, use a tiny flathead screwdriver to turn the adjustment screw so that the Current value is 4.1. • Increase the value by turning the screw clockwise.
Page 383 ADVIA 2120/2120i Operator’s Guide Troubleshooting NOTE: Be very careful to make the cut straight without burrs. Do not cut the probe on an angle. CAUTION To avoid personal injury, handle sharp items with care. 2. Replace the probe and check the length of the probe.
Page 384 Troubleshooting ADVIA 2120/2120i Operator’s Guide Regulator Acceptable Reading Set to 5 psi pressure 5 ±0.5 psi 5 +0.25 psi 40 psi pressure 40 ±2 psi 40 +1 psi NOTE: All pneumatic settings must be reached by turning the knobs in an upward or clockwise direction only.
Page 385 ADVIA 2120/2120i Operator’s Guide Troubleshooting 2. Unscrew the securing cap attached to the power cable. Perox lamp and connector securing cap Perox red lamp connector Perox lamp housing 3. Carefully pull the lamp assembly out of the optics lamp housing.
Page 386 Troubleshooting ADVIA 2120/2120i Operator’s Guide 5. Monitor the Current A/D Voltage Readings field. As you maximize the lamp, the readings number increases. 10708774 Rev. 04...
Page 387 ADVIA 2120/2120i Operator’s Guide Troubleshooting If the Current A/D Voltage screen is green, continue maximizing the lamp. Do not stop the maximizing procedure until the highest A/D number is achieved. Securing cap Red power connector Alignment screw (side) Alignment screw (top) Securing screws 6.
Page 388 Troubleshooting ADVIA 2120/2120i Operator’s Guide b. In small increments, turn the side alignment screw clockwise to move the lamp in a horizontal direction, then turn the top alignment screw clockwise to move the lamp in a vertical direction. Allow 5 to 10 seconds for the screen to update between each increment.
Page 389: Cleaning Procedures
ADVIA 2120/2120i Operator’s Guide Troubleshooting 13. Run the Normal Control and evaluate the results for Neut X and Neut Y. If the values are out of range, adjust the Gains or contact your local technical support provider for assistance. If you have any questions or concerns regarding this document, contact your local technical support provider or distributor.
Page 390 Regular strength household bleach is 5% sodium hypochlorite. Extra- strength household bleach is 6% sodium hypochlorite. Either strength may be used with the ADVIA 2120/2120i Hematology System. When using or handling ADVIA EZ Wash, bleach, any cleaning or antiviral agent, or any other potentially hazardous liquids, wear protective clothing, gloves, and safety glasses.
Page 391 ADVIA 2120/2120i Operator’s Guide Troubleshooting 4. Attach the syringe to the bottom of the filter. 5. Insert the filter into the beaker of bleach solution. 6. Gently pull the syringe plunger up, drawing fluid through the filter. Ensure the direction of flow is the opposite of the flow during normal operation.
Page 392 Troubleshooting ADVIA 2120/2120i Operator’s Guide Cleaning the Perox Chamber BIOHAZARD All products or objects that come in contact with human or animal body fluids should be handled, before and after cleaning, as if capable of transmitting infectious diseases. Wear facial protection, gloves, and protective clothing.
Page 393 ADVIA 2120/2120i Operator’s Guide Troubleshooting 2. Dip a clean, cotton-tipped swab into EZ Wash and wipe clean the inside of the perox chamber. CAUTION Avoid pushing any dirt into the drain and reagent ports at the base and back wall of the chamber. Pushing dirt into the drain and reagent ports can cause blockage.
Page 394 Troubleshooting ADVIA 2120/2120i Operator’s Guide 3. Remove the tubing from the V45 nipple on the Autosampler centering collar. Valve Valve Nipple Valve 4. Place the end of the tube into a beaker of 25% solution of household bleach and water.
Page 395 Regular strength household bleach is 5% sodium hypochlorite. Extra- strength household bleach is 6% sodium hypochlorite. Either strength may be used with the ADVIA 2120/2120i Hematology System. When using or handling ADVIA EZ Wash, bleach, any cleaning or antiviral agent, or any other potentially hazardous liquids, wear protective clothing, gloves, and safety glasses.
Page 396 Troubleshooting ADVIA 2120/2120i Operator’s Guide Inspecting the Perox-Cap Vent Hole for Buildup Overflow tube 1. Disconnect the overflow tube from the perox vent. 2. Remove the cap (2). 3. Look at the inside of the cap and look through the vent opening. Make sure that the cap and the vent are clean, dry, and free of buildup.
Page 397 ADVIA 2120/2120i Operator’s Guide Troubleshooting Waste tubing Perox flowcell at V24 White fitting at V24 EZ Wash Sample tubing Syringe Shuttle line UFC side Perox chamber cap CAUTION When performing this procedure, do not use force on the syringe at any time.
Page 398 Troubleshooting ADVIA 2120/2120i Operator’s Guide BIOHAZARD All products or objects that come in contact with human or animal body fluids should be handled, before and after cleaning, as if capable of transmitting infectious diseases. Wear facial protection, gloves, and protective clothing.
Page 399 ADVIA 2120/2120i Operator’s Guide Troubleshooting b. Remove the cap from the Perox chamber then push the syringe to fill the chamber. c. When the chamber is full, open V7 to drain the chamber. d. Close V7. e. Repeat with deionized water.
Page 400 Troubleshooting ADVIA 2120/2120i Operator’s Guide Waste tubing RBC/BASO/RETIC Flowcell White fitting at V23 Cleaning syringe Sample tubing RBC/BASO/RETIC Sample Syringe RBC Reaction Chamber BASO Reaction Chamber RETICS Reaction Chamber 1 0 Shuttle line - RBC/BASO/RETIC Vacuum Shuttle Line 1 1 Side of UFC 1.
Page 401 ADVIA 2120/2120i Operator’s Guide Troubleshooting 4. Refill the syringe with bleach solution as needed during the following steps. 5. Flush the RBC/BASO/RETIC flowcell. a. Disconnect the sample tubing from the top of the RBC/BASO/RETIC sample syringe and place the sample tubing into a beaker to catch fluid as you backflush the flowcell.
Page 402: Repair And Replacement
Troubleshooting ADVIA 2120/2120i Operator’s Guide 13. Refill the syringe with bleach solution. 14. Flush the RBC/BASO/RETIC flowcell. a. Gently push the plunger on the cleaning syringe to flush bleach solution through the RBC/BASO/RETIC flowcell. b. Repeat with deionized water. c. Reattach the shuttle line to the side of the UFC.
Page 403 ADVIA 2120/2120i Operator’s Guide Troubleshooting Fitting and tube assembly Selector valve 1. Unscrew the fitting and tube assembly from the front of the selector valve. Silicone sleeve Clot filter Filter adapter fitting 2. Gently push the silicone sleeve to release the clot filter from the filter adapter fitting.
Page 404 10. If air bubbles still appear, disconnect the filter adapter fitting and check for dust or debris in the fitting and in the port. If air bubbles persist, call your local Siemens Healthineers Customer Care Center or your local Siemens Healthineers technical support representative.
Page 405 ADVIA 2120/2120i Operator’s Guide Troubleshooting 1. Remove the reagent bottles from the side where you are replacing the diaphragm pump. 2. Pull the pump forward until the pump releases from the keyhole slots. 3. Remove the 3 tubes, then attach the tubes to the new pump.
Page 406 Troubleshooting ADVIA 2120/2120i Operator’s Guide Replacing the Drain Filters If the filter is not clogged but there is heavy buildup or discoloration, perform the backflush procedure. Materials Required Upper fitting Lower Fitting Time: 10 minutes Analyzer mode: Off WARNING The analyzer must be off; otherwise, personal injury from the needle may occur.
Page 407 ADVIA 2120/2120i Operator’s Guide Troubleshooting Replacing the Reagent Line Check Materials Required Check Valve Time: Installation and Checkout - 15 minutes Materials required: Check valve Analyzer mode: Standby. BIOHAZARD All products or objects that come in contact with human or animal body fluids should be handled, before and after cleaning, as if capable of transmitting infectious diseases.
Page 408: Replacing The Fuses
Troubleshooting ADVIA 2120/2120i Operator’s Guide Replacing the Fuses CAUTION All fuses must be replaced with same rated fuses. Replacing with an incorrect ruse can cause system damage. Fuse ratings Application Rating Number Device Type 100 and 120 220, 230, and 240...
Page 409 ADVIA 2120/2120i Operator’s Guide Troubleshooting Replacing the 20-mm fuses (F3 through F7) 1. Loosen the fuse holder by turning the holder counterclockwise. 2. Pull out the fuse holder, then remove the faulty fuse. 3. Insert the new fuse, slide the fuse holder in, and tighten the fuse holder by turning clockwise.
Page 410 Troubleshooting ADVIA 2120/2120i Operator’s Guide • the baseline value cannot be adjusted to between 3.0 and 4.1 volts. • indicated during troubleshooting. Hemoglobin lamp Adjust the baseline value: • After replacing the lamp. • If the baseline value is >4.2 volts.
Page 411 ADVIA 2120/2120i Operator’s Guide Troubleshooting Analyzer mode: Off ELECTRICAL WARNING To avoid exposure to shock hazards and/or damage to the instrument while performing this procedure, power off the analyzer before proceeding. 1. Remove the screw that secures the lamp adapter cap.
Page 412 Troubleshooting ADVIA 2120/2120i Operator’s Guide 6. Reconnect the cables. 7. Replace the lamp-adapter cap and secure the cap with the screw. 8. Turn the analyzer on. 9. Check that there are no error messages on the Status line relating to hemoglobin colorimeter baseline readings.
Page 413 ADVIA 2120/2120i Operator’s Guide Troubleshooting Replacing the Open-Tube Sampler Probe Materials Required Sample probe assembly Scalpel or single-edge razor Time: Replacement - 5 minutes Checkout - 15 minutes Analyzer mode: Off BIOHAZARD All products or objects that come in contact with human or animal body fluids should be handled, before and after cleaning, as if capable of transmitting infectious diseases.
Page 414 Troubleshooting ADVIA 2120/2120i Operator’s Guide 6. Carefully insert the probe through the opening of the probe mount and into the opening of the wash block. 7. Replace the threaded fitting and finger tighten by rotating the fitting clockwise. 8. Check the length of the sample probe and adjust if necessary. (See Adjusting the Length of the Sample Probe, page 382 for instructions.).
Page 415 ADVIA 2120/2120i Operator’s Guide Troubleshooting Analyzer mode: Off BIOHAZARD All products or objects that come in contact with human or animal body fluids should be handled, before and after cleaning, as if capable of transmitting infectious diseases. Wear facial protection, gloves, and protective clothing.
Page 416 Troubleshooting ADVIA 2120/2120i Operator’s Guide 7. At the Vacuum and Pressure area, check that the 20 in. Hg reading is between 19 and 21. 8. If the reading is not within this range, adjust the 20 in. Hg vacuum regulator knob.
Page 417: Troubleshooting Tips
ADVIA 2120/2120i Operator’s Guide Troubleshooting 5. Align the tabs on the wash block with the openings on the paddle assembly. 6. Remove the block by gently pulling the block straight out. 7. Mark the tubes with tape (the top line is rinse and the bottom line is vacuum), then remove the lines.
Page 418: Compressor Does Not Start
Troubleshooting ADVIA 2120/2120i Operator’s Guide This error sometimes displays when a rack is waiting in the input shuttle area. Move the rack back to the first position in the tray and start the Autosampler again. Checking Drain Filters for Clogs See Replacing the Drain Filters, page 406.
Page 419 ADVIA 2120/2120i Operator’s Guide Troubleshooting Data Manager and LIS At the host computer, how can I tell controls apart? In the SID that is sent to the host, the first 2 digits after the C identify the control as follows:...
Page 420 Troubleshooting ADVIA 2120/2120i Operator’s Guide 10708774 Rev. 04...
Page 421 Laboratory personnel are alerted to suspected sample abnormalities through the combined use of morphology and quantitative flags (high and low). The ADVIA 2120/2120i system morphology flags are derived from a series of complex algorithms. These algorithms are based on the identification of conditions surrounding the presence of abnormalities.
Page 422 Morphology Flags ADVIA 2120/2120i Operator’s Guide Flag Triggering Criteria CBC / CBC / CBC / RETIC DIFF DIFF / RETIC RETIC     HYPER %HYPER ≥ 4%     HYPO %HYPO ≥ 4%   [(%NEUT + %EOS) - %PMN] ≥...
Page 423 ADVIA 2120/2120i Operator’s Guide Morphology Flags Results Flagged None Possible Causes The following list of sample-related causes may not contain all conditions that could cause this flag. Multiple occurrences of this flag, especially for consecutive samples, can indicate a system problem. However, isolated instances of this flag are usually sample related.
Page 424 Morphology Flags ADVIA 2120/2120i Operator’s Guide Perox Cytogram (ATYP +++) Baso Cytogram 1 LUC area 2 BLASTS area Results Flagged None Possible Causes The following list of sample-related causes may not contain all conditions that could cause this flag. Multiple occurrences of this flag, especially for consecutive samples, can indicate a system problem.
Page 425 ADVIA 2120/2120i Operator’s Guide Morphology Flags Blasts (BLASTS) The presence of blasts is suspected. Blasts are detected in the Blasts area on the Baso cytogram and in the LUC area of the Perox cytogram. The triggering conditions for this flag are one of the following: •...
Page 426 Morphology Flags ADVIA 2120/2120i Operator’s Guide Possible Causes The following list of sample-related causes may not contain all conditions that could cause this flag. Multiple occurrences of this flag, especially for consecutive samples, can indicate a system problem. However, isolated instances of this flag are usually sample related.
Page 427 ADVIA 2120/2120i Operator’s Guide Morphology Flags Possible Causes The following list of sample-related causes may not contain all conditions that could cause this flag. Multiple occurrences of this flag, especially for consecutive samples, can indicate a system problem. However, isolated instances of this flag are usually sample related.
Page 428 Morphology Flags ADVIA 2120/2120i Operator’s Guide Multiple occurrences of this flag, especially for consecutive samples, can indicate a system problem. However, isolated instances of this flag are usually sample related. Sample Related System Related • Hereditary 1. Check RBC reagents.
Page 429 ADVIA 2120/2120i Operator’s Guide Morphology Flags Multiple occurrences of this flag, especially for consecutive samples, can indicate a system problem. However, isolated instances of this flag are usually sample related. Sample Related System Related • Iron deficiency anemia 1. Check RBC reagents.
Page 430 Morphology Flags ADVIA 2120/2120i Operator’s Guide Perox Cytogram Baso Cytogram Lymph Mono Neut MN (6) = Lymphs (1) + LUCs (2) + Monos (3) PMN (7) = Neut (4) + Eos (5) Results Flagged None Possible Causes The following list of sample-related causes may not contain all conditions that could cause this flag.
Page 431 ADVIA 2120/2120i Operator’s Guide Morphology Flags Large Platelets (LPLT) The Large Platelets flag triggers if the percentage of large platelets (%LPLT) is greater than 10% of the platelet count. The %LPLT parameter indicates the percent of platelets with volumes greater than 20 fL. This parameter is derived from the Platelet Volume histogram based on Integrated Analysis.
Page 432 Morphology Flags ADVIA 2120/2120i Operator’s Guide For normal samples, the valley separating the MN and PMN populations is well defined, as determined by its relative depth (d/D). If this valley becomes too shallow (d/D < 0.15), the MN and PMN populations cannot be separated.
Page 433 ADVIA 2120/2120i Operator’s Guide Morphology Flags Possible Causes The following list of sample-related causes may not contain all conditions that could cause this flag. Multiple occurrences of this flag, especially for consecutive samples, can indicate a system problem. However, isolated instances of this flag are usually sample related.
Page 434 Morphology Flags ADVIA 2120/2120i Operator’s Guide Multiple occurrences of this flag, especially for consecutive samples, can indicate a system problem. However, isolated instances of this flag are usually sample related. Sample Related System Related • Macrocytic anemia 1. Check RBC reagents.
Page 435 ADVIA 2120/2120i Operator’s Guide Morphology Flags Possible Causes The following list of sample-related causes may not contain all conditions that could cause this flag. Multiple occurrences of this flag, especially for consecutive samples, can indicate a system problem. However, isolated instances of this flag are usually sample related.
Page 436 Morphology Flags ADVIA 2120/2120i Operator’s Guide You must perform a manual differential on samples that trigger the Myeloperoxidase Deficiency flag. Normal Peroxidase Staining Weak Peroxidase Staining (MPO flag) Perox Cytogram Perox Cytogram Baso Cytogram Baso Cytogram Because %PMN - (%NEUT...
Page 437 ADVIA 2120/2120i Operator’s Guide Morphology Flags NEUT area EOS area PMN area Results Flagged %NEUT, #NEUT, %LYMPH, #LYMPH, %MONO, #MONO, %EOS, #EOS, %BASO, #BASO, %LUC, and #LUC Possible Causes The following list of sample-related causes may not contain all conditions that could cause this flag.
Page 438 Morphology Flags ADVIA 2120/2120i Operator’s Guide Multiple occurrences of this flag, especially for consecutive samples, can indicate a system problem. However, isolated instances of this flag are usually sample related. Sample Related System Related • Neonatal samples 1. Check perox reagents.
Page 439 ADVIA 2120/2120i Operator’s Guide Morphology Flags Perox Cytogram Perox Cytogram Normal Sample PLT-CLM flag PLT Clumps area Results Flagged WBCP, PLT, PCT, MPV, and PDW Possible Causes The following list of sample-related causes may not contain all conditions that could cause this flag.
Page 440 Morphology Flags ADVIA 2120/2120i Operator’s Guide RBC Fragments (RBCF) The presence of RBC fragments is suspected. This flag triggers if the number of events in the RBC Fragment area of the PLT Scatter cytogram is greater than 100,000 cells/ìL. The event count in this area includes red blood cell fragments with volumes less than 30fL and refractive indexes greater than 1.400.
Page 441 ADVIA 2120/2120i Operator’s Guide Morphology Flags Possible Causes The following list of sample-related causes may not contain all conditions that could cause this flag. Multiple occurrences of this flag, especially for consecutive samples, can indicate a system problem. However, isolated instances of this flag are usually sample related.
Page 442 Morphology Flags ADVIA 2120/2120i Operator’s Guide The RBC Ghost value is intended for flagging and laboratory purposes only. Results Flagged None Possible Causes The following list of sample-related causes may not contain all conditions that could cause this flag. Multiple occurrences of this flag, especially for consecutive samples, can indicate a system problem.
Page 443 ADVIA 2120/2120i Operator’s Guide Morphology Flags Flag Triggering Criteria CBC / CBC / CBC / RETIC DIFF DIFF / RETIC RETIC B-SUSP Events above the upper MN/PMN   (BC) threshold on y axis and in channels 0 to 49 on x axis . 5% BIFR Baso Flatnes >...
Page 444 Morphology Flags ADVIA 2120/2120i Operator’s Guide Flag Triggering Criteria CBC / CBC / CBC / RETIC DIFF DIFF / RETIC RETIC    NRLPLT LPLT 40 x 10 / µL (NP)   NRPXNV No valley between the NRBC and lymphocyte populations and a reportable NRBC count.
Page 445 ADVIA 2120/2120i Operator’s Guide Morphology Flags Flag Triggering Criteria CBC / CBC / CBC / RETIC DIFF DIFF / RETIC RETIC     RBCIFR RBC Flatness > 3.2 (RR)    RTCint Platelet threshold below channel 5 (CT) ...
Page 446 Morphology Flags ADVIA 2120/2120i Operator’s Guide Flag Triggering Criteria CBC / CBC / CBC / RETIC DIFF DIFF / RETIC RETIC   WBC-CE 1. (WBCB - WBCP) > 1.0 x 10 / µL (WC) when WBCB > 2.0 x 10 / µL and...
Page 447 ADVIA 2120/2120i Operator’s Guide Morphology Flags When histogram analysis is applied, the Baso Count Suspect region is located above channel 21 on the y axis and in channels 0 and 49 on the x axis. BASO Cytogram Baso Cytogram (cluster analysis)
Page 448 Morphology Flags ADVIA 2120/2120i Operator’s Guide The following list of sample-related causes may not contain all conditions that could cause this flag. There is no intention to associate the flag with specific diagnoses. Sample Related System Related • High WBC Count 1.
Page 449 ADVIA 2120/2120i Operator’s Guide Morphology Flags 3. Check sheath delivery. A partially clogged RBC/baso/retic sheath filter can produce a distinctive “ski slope” effect on the RBC, Baso, and Retic flow rate histograms. Replace the sheath filter. RBC Rate Baso Rate...
Page 450 Morphology Flags ADVIA 2120/2120i Operator’s Guide If histogram analysis is applied, the Noise area (2) is located between channels 0 and 8 on the y axis. Cluster Analysis Histogram Analysis Noise cluster Noise area Results Flagged WBC, WBCB, %BASO, #BASO...
Page 451 ADVIA 2120/2120i Operator’s Guide Morphology Flags Sample Related System Related • Malaria parasites Check sheath delivery. • Aged Blood Check the baso reaction chamber temperature. Check RBC/Baso/Retic flowcell alignment. Baso No Valley (B-NV, VB) The Baso No Valley flag triggers if there is no valid separation between themononuclear (MN) and polymorphonuclear (PMN) populations in the Baso cytogram.
Page 452 Morphology Flags ADVIA 2120/2120i Operator’s Guide Normal B-N (VB) Flag MN Peak PMN Peak A MN/PMN Valley Results Flagged Corrective Action Multiple occurrences of this flag, especially for consecutive samples, can indicate a system problem. However, isolated instances of this flag are usually sample related.
Page 453 ADVIA 2120/2120i Operator’s Guide Morphology Flags The following list of sample-related causes may not contain all conditions that could cause this flag. There is no intention to associate the flag with specific diagnoses. Sample Related System Related • Aged blood 1.
Page 454 Morphology Flags ADVIA 2120/2120i Operator’s Guide The following rules determine if the WBCB or WBCP count is reported for a CBC/Diff sample: • WBCB is the primary WBC count. • If B-NO or B-SAT flags are triggered, the WBCP count is reported if valid.
Page 455 ADVIA 2120/2120i Operator’s Guide Morphology Flags Results Flagged WBC, WBCB, %BASO, #BASO, %LYMPH, #LYMPH A 4-part WBC differential (basophils excluded) is reported with the measured BASO values displayed and flagged, and with the LYMPH and LUC values also flagged. Corrective Action 1.
Page 456 Morphology Flags ADVIA 2120/2120i Operator’s Guide Sample Related System Related Neonatal (NRBCs) a. If all results are acceptable, calibrate RBC, MCV, CHCM, and HGB parameters Hemolytic anemias b. If the HGB results are faulty, troubleshoot the hemoglobin channel. Cold agglutinins c.
Page 457 ADVIA 2120/2120i Operator’s Guide Morphology Flags The following list of sample-related causes may not contain all conditions that could cause this flag. There is no intention to associate the flag with specific diagnoses. Sample Related System Related Incomplete red cell lysis 1.
Page 458 Morphology Flags ADVIA 2120/2120i Operator’s Guide Hgb Power Low (HGB-PL, PH) The Hgb Power Low flag triggers if the HGB baseline transmission is less than 2.5 or greater than 4.1. Results Flagged HGB, MCH, and MCHC Corrective Action 1. Adjust the Hgb baseline.
Page 459 ADVIA 2120/2120i Operator’s Guide Morphology Flags No Perox NRBC / Lymph Valley (NRPXNV, NV) The No Perox NRBC / Lymph Valley flag is triggered if there is no valley between the nRBC and lymphocyte populations when the system reports an NRBC count.
Page 460 Morphology Flags ADVIA 2120/2120i Operator’s Guide The Perox Rate Histogram displays the arrival rate of the cells in the perox channel. Normal Perox Rate Histogram PXIFR Perox Rate Histogram Results Flagged WBCP, %NEUT, #NEUT, %LYMPH, #LYMPH, %MONO, #MONO, %EOS, #EOS,...
Page 461 ADVIA 2120/2120i Operator’s Guide Morphology Flags In normal samples, PEROX d/D is greater than 0.15. This flag is triggered if PEROX d/D is less than 0.15. Normal PX-NV (VX) Flag Noise-Lymph Noise-Lymph Noise Peak Lymph Peak The PX-NV (VX) flag is not triggered if agreement between the baso and perox results is indicated by both the following conditions: •...
Page 462 Morphology Flags ADVIA 2120/2120i Operator’s Guide Corrective Action Multiple occurrences of this flag, especially for consecutive samples, can indicate a system problem. However, isolated instances of this flag are usually sample related. The following list of sample-related causes may not contain all conditions that could cause this flag.
Page 463 ADVIA 2120/2120i Operator’s Guide Morphology Flags This flag is triggered if 60% or more of the PEROX events are counted in the Noise area. Normal PX-NO Perox Cytogram Perox Cytogram Noise area The PX-NO (NX) flag is not triggered if agreement between the baso and perox results is indicated by both the following conditions: •...
Page 464 Morphology Flags ADVIA 2120/2120i Operator’s Guide Results Flagged WBCP, %NEUT, #NEUT, %LYMPH, #LYMPH, %MONO, #MONO, %EOS, #EOS, %LUC, and #LUC Corrective Action Multiple occurrences of this flag, especially for consecutive samples, can indicate a system problem. However, isolated instances of this flag are usually sample related.
Page 465 ADVIA 2120/2120i Operator’s Guide Morphology Flags Perox Saturation (PX-SAT, XS) The Perox Saturation flag triggers if the events counted in the Saturation area are more than 10% of Perox signals The Saturation area of the Perox cytogram is located in channels 97 through 99 on the x axis.
Page 466 Morphology Flags ADVIA 2120/2120i Operator’s Guide The following list of sample-related causes may not contain all conditions that could cause this flag. There is no intention to associate the flag with specific diagnoses. Sample Related System Related Immature granulocytes 1. Check perox reagents.
Page 467 ADVIA 2120/2120i Operator’s Guide Morphology Flags Platelet Noise (PLT-NO, NT) The PLT-NO flag indicates the presence of sample interferences such as microcytes or RBC fragments that could be counted as large platelets The PLT-NO flag is triggered under either of the following conditions: •...
Page 468 Morphology Flags ADVIA 2120/2120i Operator’s Guide Platelet Origin Noise (PLTORN, OT) The Platelet Origin Noise flag indicates the occurrence of system-induced noise that can affect RBC and PLT results. The PLTORN flag triggers when the number of events in the Platelet Origin Noise area of the PLT cytogram exceeds 2% of the total number of events.
Page 469 ADVIA 2120/2120i Operator’s Guide Morphology Flags RBC Irregular Flow Rate (RBCIFR, RR) The RBC Irregular Flow Rate flag is triggered if the cell counting rate is erratic because of a hydraulic disturbance in the RBC/PLT channel. RBC flow rate is evaluated in terms of the cell counting rate: The flag is triggered if this value is greater than 3.2.
Page 470 Morphology Flags ADVIA 2120/2120i Operator’s Guide RBC Rate Baso Rate Retic Rate Retic – Plt Interference Error (RTCint, CT) The RTCint flag is triggered if the platelet threshold is set below scatter channel 5. If the flag is triggered, platelets may be counted in the gated mature RBC population.
Page 471 ADVIA 2120/2120i Operator’s Guide Morphology Flags The SD of the absorption histogram above Retic Abs 80% of the original histogram height is greater than 1.4. The measured mean absorption minus the Retic Abs F theoretical absorption is greater than 30%.
Page 472 Morphology Flags ADVIA 2120/2120i Operator’s Guide Corrective Action Laser oscillation (also called mode hopping) is defined as a change in the laser output frequency during the analysis. Typically, this condition triggers 3 sample/system flags. • RETIC Fit Suspect flag (RTC-FS) is triggered due to the bimodal absorption populations that appear on the RETIC Scatter Absorption cytogram and the RETIC Absorption histogram.
Page 473 ADVIA 2120/2120i Operator’s Guide Morphology Flags Results Flagged %RETIC, #RETIC, CHg, CHr, CHCMg, CHCMr, CHDWg, CHDWr, MCVg, MCVr, RDWg, RDWr Corrective Action The following list may not contain all conditions that could cause this flag, nor is there any intention to associate the flag with specific diagnoses.
Page 474 Morphology Flags ADVIA 2120/2120i Operator’s Guide 5. Check the pressure and vacuum readings. A partially clogged RBC/baso/retic sheath filter can produce a distinctive “ski slope” effect on the RBC, Baso, and Retic flow rate histograms. Replace the sheath filter. RBC Rate...
Page 475 ADVIA 2120/2120i Operator’s Guide Morphology Flags Results Flagged %RETIC, #RETIC, CHg, CHr, CHCMg, CHCMr, CHDWr, CHDWg, MCVg, MCVr, RDWg, RDWr Corrective Action Multiple occurrences of this flag, especially for consecutive samples, can indicate a system problem. However, isolated instances of this flag are usually sample related.
Page 476 Morphology Flags ADVIA 2120/2120i Operator’s Guide The following list of sample-related causes may not contain all conditions that could cause this flag. There is no intention to associate the flag with specific diagnoses. Sample Related System Related Anemia 1. Insufficient sample 2.
Page 477 ADVIA 2120/2120i Operator’s Guide Morphology Flags Corrective Action Multiple occurrences of this flag, especially for consecutive samples, can indicate a system problem. However, isolated instances of this flag are usually sample related. The following list of sample-related causes may not contain all conditions that could cause this flag.
Page 478 Morphology Flags ADVIA 2120/2120i Operator’s Guide Results Flagged %RETIC, #RETIC, CHg, CHr, CHCMg, CHCMr, CHDWr, CHDWg, MCVg, MCVr, RDWg, RDWr Corrective Action The following list may not contain all conditions that could cause this flag, nor is there any intention to associate the flag with specific diagnoses.
Page 479 ADVIA 2120/2120i Operator’s Guide Morphology Flags Suspect Large Pit Interference (NRLPLT, NP) The Suspect Lipid Interference flag is triggered if the system counts more than 40,000 large platelets /µL in the RBC/PLT channel. Results Flagged #NRBC, %NRBC, WBC, %NEUT, #NEUT, %LYMPH, #LYMPH, %MONO,...
Page 480 Morphology Flags ADVIA 2120/2120i Operator’s Guide The Baso cytogram and NRBC Enumeration Histogram below illustrate the NRLPD flag. The circled area of the Baso cytogram identifies the lipid pattern. BASO Cytogram NRBC Enumeration Histogram WBC Substitution (WBCSUB, WS) The WBC substitution rules for CBC / DIFF samples are as follows: •...
Page 481: Error Messages
8 Error Messages % Disk Space Used - Alarm The amount of disk space used has risen to the level specified as an alarm criterion, indicating available disk space is low. Increase available disk space by deleting data from any of these files: •...
Page 482 Error Messages ADVIA 2120/2120i Operator’s Guide 20 PSI Out of Range System pressure is out of range. Possible Cause Corrective Action PSI pressure Check the 20 PSI gauge and adjust the regulator if gauge is out of necessary. range SETpoint: 20 PSI ± 1.
Page 483 ADVIA 2120/2120i Operator’s Guide Error Messages 4 Key Not Found The Control Dictionary or the Alarm Dictionary is empty. If the Alarm Dictionary is empty, sample and system flags appear as exclamation points (!). Possible Cause Corrective Action The Data Manager...
Page 484 Error Messages ADVIA 2120/2120i Operator’s Guide 40 PSI Out of Range System pressure is out of range. Possible Cause Corrective Action 40 PSI pressure gauge is Check the 40 PSI gauge and adjust the out of range regulator, if necessary.
Page 485 ADVIA 2120/2120i Operator’s Guide Error Messages 5 PSI Out of Range System pressure is out of range. Possible Cause Corrective Action 5 PSI pressure gauge Check the 5 PSI gauge and adjust the is out of range regulator, if necessary.
Page 486: End Of File
Error Messages ADVIA 2120/2120i Operator’s Guide Corrective Action Wait for a few minutes and try accessing the sample again. 9 End of File The Control Dictionary is empty. Quality Control cannot be operated. Corrective Action 1. Select Control Dictionary. 2. Define at least 1 QC file.
Page 487 2. After about 1 minute, if the status line does not display Ready to Run and the green ready-to-aspirate light is still off, follow these steps to exit the ADVIA 2120/ 2120i software, turn off the analyzer and then restart.
Page 488 Ready to Run and the node, pressure node, green ready-to-aspirate light is still off, parallel node, switch follow these steps to exit the ADVIA 2120/ indicator, or selector 2120i software, turn off the analyzer and valve then restart.
Page 489 The computer Follow these steps to exit the networking ADVIA 2120/2120i software, turn off the configuration has analyzer, and then restart: been corrupted. 1. Select Shut Down ADVIA in the Log On/ Off window. 2. Select Off at the analyzer touchpad.
Page 490 Error Messages ADVIA 2120/2120i Operator’s Guide Aspiration Failed - Probe Clog - Alarm Conductivity detectors did not detect a sample within the 20 seconds of aspiration. The number of consecutive occurrences of this condition has met the criterion set for an alarm message.
Page 491 ADVIA 2120/2120i Operator’s Guide Error Messages Aspiration Failed - Probe Clog - Stop Conductivity detectors did not detect a sample within the 20 seconds of aspiration. The number of consecutive occurrences of this condition has met the criterion set for a stop message.
Page 492 Error Messages ADVIA 2120/2120i Operator’s Guide Aspiration Paused The analyzer is unable to perform a sample aspiration or to start a hydraulic cycle when certain menus or tabs are open or when certain error messages are triggered. Also the green ready-to-aspirate light is off.
Page 493 ADVIA 2120/2120i Operator’s Guide Error Messages Auto Rinse Canceled The System canceled the scheduled Auto Rinse in accordance with user cancellation. Auto Rinse Missed The scheduled Auto Rinse was missed and did not occur while the Exerciser or System Setup tab was open.
Page 494 Error Messages ADVIA 2120/2120i Operator’s Guide Auto System Wash - Exiting Standby The System moved from Standby to Ready-to-Run, enabling the scheduled Auto System Wash. Auto System Wash Canceled The System canceled the scheduled Auto System Wash in accordance with user cancellation.
Page 495 ADVIA 2120/2120i Operator’s Guide Error Messages Corrective Action 1. Replenish expired Regents. 2. Select Logs > Reagent Log > Reagent Installation. 3. Update Reagent Installation. autoRETIC Reagent Low - Alarm The number of consecutive occurrences of this condition has met the criterion specified for an alarm message, indicating that the reagent level is low.
Page 496 Error Messages ADVIA 2120/2120i Operator’s Guide Autosampler - Bad Mixer Aspiration Cal Mixer-aspiration calibration failed in diagnostic mode (Exerciser tab). NOTE: Contact your local technical support provider for assistance. If you have any questions or concerns regarding this document, contact your local technical support provider or distributor.
Page 497 ADVIA 2120/2120i Operator’s Guide Error Messages If you have any questions or concerns regarding this document, contact your local technical support provider . If you have any questions or concerns regarding this document, contact your local technical support provider or distributor.
Page 498 Error Messages ADVIA 2120/2120i Operator’s Guide Autosampler - Car Lost Steps, Reset Autosampler The Autosampler car is not in the expected position. A reset is required. Possible Cause Corrective Actions Car movement problem. 1. On the analyzer touchpad, select Off.
Page 499 ADVIA 2120/2120i Operator’s Guide Error Messages Autosampler - Car Motion Denied, Reset Autosampler The Autosampler attempted an inappropriate car motion. An Autosampler reset is required. Corrective Action 1. Select Off at the analyzer touchpad. 2. Wait 1 minute. 3. Select On to restart the analyzer.
Page 500 Error Messages ADVIA 2120/2120i Operator’s Guide Autosampler - Centering Collar Failed to Extend, Reset Autosampler The Autosampler centering collar failed to extend properly. Possible Causes Corrective Action Critical Sampler Error. 1. Open the Autosampler access door. Partial Reset is required.
Page 501 ADVIA 2120/2120i Operator’s Guide Error Messages Autosampler - Centering Collar Failed to Retract, Reset Autosampler The Autosampler centering collar failed to retract properly. Possible Causes Corrective Actions Critical Sampler Error. 1. Open the Autosampler access door. Partial Reset is required.
Page 502 Error Messages ADVIA 2120/2120i Operator’s Guide Autosampler - Collar Failed to Retract, Reset Autosampler The Autosampler centering collar failed to retract properly. Possible Causes Corrective Actions Critical Sampler Error. 1. Open the Autosampler access door. Partial Reset is required. 2. Close the Autosampler access door.
Page 503 ADVIA 2120/2120i Operator’s Guide Error Messages Autosampler - Centering Collar Motion Denied, Reset Autosampler The Autosampler attempted an inappropriate centering collar motion. An Autosampler reset is required. Corrective Action 1. Select Off at the analyzer touchpad. 2. Wait about 1 minute and select On to restart the analyzer.
Page 504 Error Messages ADVIA 2120/2120i Operator’s Guide Autosampler - Eject Rack Failed The Autosampler failed to respond to the eject rack command and is not clearing racks from the mixer and input and output shuttles. Possible Cause Corrective Action Command from 1.
Page 505 ADVIA 2120/2120i Operator’s Guide Error Messages Autosampler - Error, Reset Autosampler The Autosampler has experienced a software error and must be reset. Corrective Action 1. At the analyzer touchpad, select Off. 2. Wait about 1 minute and select On to restart the analyzer.
Page 506 Error Messages ADVIA 2120/2120i Operator’s Guide Autosampler - Initialization Required In diagnostic mode, the analyzer sent a position command before the appropriate initialization was performed. For example, a position mixer command could have been sent before a mixer initialization. Corrective Action...
Page 507 ADVIA 2120/2120i Operator’s Guide Error Messages 3. If the problem persists, contact your local technical support provider for assistance If you have any questions or concerns regarding this document, contact your local technical support provider or distributor. Autosampler - Input Queue Motion Failed The Autosampler input queue failed to move properly.
Page 508 Error Messages ADVIA 2120/2120i Operator’s Guide Autosampler - Input Queue Motion Failed, Reset Autosampler The Autosampler input queue failed to move properly. An Autosampler reset is required. Possible Cause Corrective Action Rack has jammed the 1. Remove and replace rack.
Page 509 ADVIA 2120/2120i Operator’s Guide Error Messages Corrective Action 1. Remove the rack from the shuttle position at the front of the input queue. 2. Select Start on the analyzer touchpad to restart Autosampler operation. Autosampler - Internal Communication Failure During normal operation, the Autosampler detected an internal, software- level communication failure.
Page 510 Error Messages ADVIA 2120/2120i Operator’s Guide Autosampler - Invalid Sequence Number The Autosampler sent the analyzer a sample ID sequence number that did not match the expected sequence number. Possible Cause Corrective Action The Autosampler 1. At the analyzer touchpad, select Off.
Page 511 ADVIA 2120/2120i Operator’s Guide Error Messages Autosampler - Mixer Lost Steps, Reset Autosampler The Autosampler mixer is not in the expected position. A reset is required. Possible Cause Corrective Action Car movement problem. 1. At the analyzer touchpad, select Off.
Page 512 Error Messages ADVIA 2120/2120i Operator’s Guide 4. If the problem persists, contact your local technical support provider for assistance. If you have any questions or concerns regarding this document, contact your local technical support provider or distributor. Autosampler - Mixer Motion Failed, Reset Autosampler The Autosampler failed to perform a routine mixer motion during normal rack processing.
Page 513 ADVIA 2120/2120i Operator’s Guide Error Messages Autosampler - Mode of Operation Not Set The Autosampler was unable to carry out a requested motion while the system was in the Exerciser tab. In the Exerciser tab, set the Autosampler to the correct mode for the operation you want to perform.
Page 514 Error Messages ADVIA 2120/2120i Operator’s Guide 2. Wait 1 minute. 3. Select On to restart the analyzer. If the problem persists, contact your local technical support provider for assistance If you have any questions or concerns regarding this document, contact your local technical support provider or distributor.
Page 515 ADVIA 2120/2120i Operator’s Guide Error Messages Autosampler - Needle Failed to Extend, Reset Autosampler The Autosampler needle failed to extend properly. Possible Cause Corrective Action Critical Sampler Error. 1. Open the Autosampler access door. 2. Close the Autosampler access door.
Page 516 Error Messages ADVIA 2120/2120i Operator’s Guide Autosampler - Needle Failed to Retract, Reset Autosampler The Autosampler needle failed to retract properly. Possible Cause Corrective Action Critical Sampler Error. 1. Open the Autosampler access door. 2. Close the Autosampler access door.
Page 517 ADVIA 2120/2120i Operator’s Guide Error Messages Autosampler - Needle Jammed The needle failed to extend or retract during sample aspiration. Possible Cause Corrective Action 40 PSI line out of range. 1. Check pressure and adjust if necessary. 2. Check for pinched air lines supplying needle cylinder.
Page 518 Error Messages ADVIA 2120/2120i Operator’s Guide Autosampler - Needle Retract Denied To prevent the collision of Autosampler components, the system did not execute the command to retract the Autosampler needle. Autosampler - No Tube Present Pushpin cannot find tube in current rack position. The rack spring clip is broken and holds tube incorrectly.
Page 519 ADVIA 2120/2120i Operator’s Guide Error Messages Autosampler - Output Queue Full Possible Cause Corrective Action Output queue and/or Remove racks from output queue and/or output shuttle are full. output shuttle. Output queue sensor, Only your local your local technical support...
Page 520 Error Messages ADVIA 2120/2120i Operator’s Guide Autosampler - Output Queue Motion Denied To prevent the collision of Autosampler components, the system did not execute the command to move the Autosampler output queue. Information only. Autosampler - Output Queue Motion Failed The Autosampler output queue failed to move properly.
Page 521 ADVIA 2120/2120i Operator’s Guide Error Messages Autosampler - Output Shuttle Not Empty The Autosampler has attempted to move a rack to the output shuttle, but the shuttle is not empty. Corrective Action Remove rack from the output shuttle. Autosampler - Pushpin Extend Denied To prevent the collision of Autosampler components, the system did not execute the command to extend the Autosampler pushpin.
Page 522 Error Messages ADVIA 2120/2120i Operator’s Guide Autosampler - Pushpin Failed to Extend, Reset Autosampler The Autosampler pushpin failed to extend properly. Possible Cause Corrective Action Pushpin is jammed. 1. Open the Autosampler access door. 2. Close the Autosampler access door.
Page 523 ADVIA 2120/2120i Operator’s Guide Error Messages Autosampler - Pushpin Failed to Retract, Reset Autosampler The Autosampler pushpin failed to retract properly. Possible Cause Corrective Action Pushpin is jammed. 1. Open the Autosampler access door. 2. Close the Autosampler access door.
Page 524 Error Messages ADVIA 2120/2120i Operator’s Guide Autosampler - Pushpin Jammed Pushpin is jammed and cannot extend or retract properly. Possible Cause Corrective Action Pushpin catches on 1. Check and adjust rack position in mixer. bottom of improperly If the problem persists, contact your positioned rack.
Page 525 ADVIA 2120/2120i Operator’s Guide Error Messages Autosampler - Rack Index Failed, Reset Autosampler The scanner has read a valid barcode that does not correspond to the expected rack or position numbers. This indicates that the rack has not moved to the proper position.
Page 526 Error Messages ADVIA 2120/2120i Operator’s Guide Autosampler - Rack or Position Barcode Not Read The barcode reader cannot determine a valid rack ID or position number for the current tube position. Possible Cause Corrective Action Rack barcode label 1. Ensure the barcode label is correctly improperly placed, placed on the rack.
Page 527 ADVIA 2120/2120i Operator’s Guide Error Messages Possible Cause Corrective Action Window of barcode 1. Clean barcode reader window. reader is dirty, or line of 2. Ensure there is no visual obstruction sight is obstructed. between the barcode reader window and the rack.
Page 528 Error Messages ADVIA 2120/2120i Operator’s Guide Autosampler - Shuttle Gate Open Input queue cannot advance the rack because the shuttle gate is not closed. Possible Cause Corrective Action Shuttle gate only 1. Check for an obstruction to the shuttle partially closed.
Page 529 ADVIA 2120/2120i Operator’s Guide Error Messages Autosampler - Tube Too High Tube is sitting too high in rack, or tube exceeds specifications, halting Autosampler. Possible Cause Corrective Actions Tube is improperly 1. Push tube down. positioned. 2. At the analyzer touchpad, select Start/Stop to continue operations.
Page 530 Error Messages ADVIA 2120/2120i Operator’s Guide Software communication error Possible Cause Corrective Actions This error can occur Exit the ADVIA 2120/2120i software and then during the following restart: analytical cycles: 1. Select Shut Down ADVIA at the Log On/ •...
Page 531 ADVIA 2120/2120i Operator’s Guide Error Messages Only Siemens Healthineers Customer Care Center or your local Siemens technical support representative are authorized to perform Healthineers actions for this issue for assistance. Possible Cause Corrective Action Autosampler Calibration Calibrate the mixer aspirate position again.
Page 532 Error Messages ADVIA 2120/2120i Operator’s Guide The specified alarm criterion is user definable. Select Customize > System Setup > Alarm/Stop Criteria to view or modify settings. The counter associated with the Alarm Criteria automatically resets. BASO Irregular Flow Rate - Stop The number of consecutive occurrences of this condition has met the criterion specified for a stop message.
Page 533 ADVIA 2120/2120i Operator’s Guide Error Messages The alarm criterion is user configurable. Select Customize > System Setup > Alarm/Stop Criteria > Reagent Conditions to view or modify settings. The alarm counter automatically resets after replenishing reagents and updating Reagent Installation.
Page 534 Error Messages ADVIA 2120/2120i Operator’s Guide The specified stop criterion is user definable. Select Customize > System Setup > Alarm/Stop Criteria to view or modify settings. The counter associated with the Stop Criteria resets when selecting Start/ Stop. Blasts - Alarm The number of consecutive occurrences of this condition has met the criterion specified for an alarm message.
Page 535 ADVIA 2120/2120i Operator’s Guide Error Messages Check Waste Container Vacushield The vacuum pump filter (vacushield) on the waste container may be saturated with liquid. Possible Cause Corrective Action Vacuum pump filter Replace the vacuum pump filter. (vacushield) is clogged Vacuum leak 1.
Page 536 After 1 minute, if the status line does not display Ready to Run and the green ready-to-aspirate light is still off, exit the ADVIA 2120/2120i software, turn off the analyzer, and then restart the analyzer. a. Select Shut Down ADVIA at the Log On/ Off window.
Page 537 ADVIA 2120/2120i Operator’s Guide Error Messages The counter associated with the Stop Criteria resets when selecting Start/Stop. Comparison Error WBCB/WBCP - Alarm The number of consecutive occurrences of this condition has met the criterion specified for an alarm message. The specified stop criterion is user definable. Select Customize > System Setup >...
Page 538 Error Messages ADVIA 2120/2120i Operator’s Guide Possible Cause Corrective Action Vacuum line attached to 1. Reconnect or remove any crimps from the the waste container is waste container vacuum lines. disconnected or crimped 2. On the analyzer touchpad, select Off.
Page 539 Analyzer Reset. After about 1 minute, if the status line does not display Ready to Run and the green ready aspirate light is still off, exit the ADVIA 2120/2120i software, turn off the analyzer, and then restart. 1. Select Shut Down ADVIA at the Log On/Off window.
Page 540 Error Messages ADVIA 2120/2120i Operator’s Guide Defined More Than Once In File NOTE: This message can begin with a keyword or a test code. Possible Cause Corrective Action The same keyword is set Use the Format window to correct the file several times in the layout.
Page 541 ADVIA 2120/2120i Operator’s Guide Error Messages DEFOAMER Reagent Low - Alarm The number of consecutive occurrences of this condition has met the criterion specified for an alarm message, indicating the reagent level is low. The specified alarm criterion is user definable. Select Customize > System Setup >...
Page 542 Error Messages ADVIA 2120/2120i Operator’s Guide 2. Select Select Logs > Reagent Log > Reagent Installation. 3. Update Reagent Installation. EZ Wash Reagent Low - Alarm The number of consecutive occurrences of this condition has met the criterion specified for an alarm message, indicating the reagent level is low.
Page 543 ADVIA 2120/2120i Operator’s Guide Error Messages File Not In Modifiable File List - Updating This message appears when a file is created through the Format window. The file is then stored in the database. Information only. No action is required.
Page 544 Ready to Run and the green ready-to- aspirate light is still off, follow these steps to exit the ADVIA 2120/2120i software, turn off the analyzer and then restart the system. a. Select Shut Down ADVIA at the Log On/ Off window.
Page 545 ADVIA 2120/2120i Operator’s Guide Error Messages The specified stop criterion is user definable. Select Customize > System Setup > Alarm/Stop Criteria to view or modify settings. The counter associated with the Stop Criteria resets when selecting Start/ Stop. HGB Irregular Flow Rate - Alarm The number of consecutive occurrences of this condition has met the criterion specified for an alarm message.
Page 546 Error Messages ADVIA 2120/2120i Operator’s Guide HGB Reagent Empty - Stop The number of consecutive occurrences of this condition has met the criterion specified for a stop message. The reagent is empty, and the Autosampler has halted. The specified stop criterion is user definable. Select Customize > System Setup >...
Page 547 ADVIA 2120/2120i Operator’s Guide Error Messages Hyperchromia - Alarm The number of consecutive occurrences of this condition has met the criterion specified for an alarm message. The specified alarm criterion is user definable. Select Customize > System Setup > Alarm/Stop Criteria > Reagent Conditions to view or modify settings.
Page 548 After 1 minute, if the status line does not display Ready to Run and the green ready-to- aspirate light is still off, follow these steps to exit the ADVIA 2120/2120i software, turn off the analyzer and then restart the analyzer. a. Select Shut Down ADVIA at the Log On/Off window.
Page 549 Initialization Failed - During Communication from Analyzer Software communication error. Exit the ADVIA 2120/2120i software and then restart the software. 1. Select Shut Down ADVIA at the Log On/Off window. 2. Select CTRL+ALT+DELETE and log back onto the system to restart the software.
Page 550 Initialization Failed - During Download of Cycle Definition Software communication error. Exit the ADVIA 2120/2120i software and then restart the software. 1. Select Shut Down ADVIA at the Log On/Off window. 2. Select CTRL+ALT+DELETE and log back onto the system to restart the software.
Page 551 Software communication error or possible faulty Ethernet cable (from the analyzer - J2 Workstation connection to the computer). Exit the ADVIA 2120/2120i software and then restart the software. 1. Select Shut Down ADVIA at the Log On/Off window. 2. Select CTRL+ALT+DELETE and log back onto the system to restart the software.
Page 552 Error Messages ADVIA 2120/2120i Operator’s Guide Invalid Host Test Number (=0) A test has an improper host number defined in the Test Dictionary. Verify the host number in Test Dictionary, Characteristics. 1. Select Customize > Tools View. 2. On the Tools list, select Test Dictionary > Characteristics.
Page 553 ADVIA 2120/2120i Operator’s Guide Error Messages Large Platelets - Alarm The number of consecutive occurrences of this condition has met the criterion specified for an alarm message. The specified alarm criterion is user definable. Select Customize > System Setup > Alarm/Stop Criteria to view or modify settings.
Page 554 Error Messages ADVIA 2120/2120i Operator’s Guide The counter associated with the Stop Criteria is reset when the Start/Stop button is selected. Left Shift - Alarm The number of consecutive occurrences of this condition has met the criterion specified for an alarm message.
Page 555 ADVIA 2120/2120i Operator’s Guide Error Messages The specified stop criterion is user definable in the Customize Menu, System Setup, Alarm/Stop Criteria. The counter associated with the Stop Criteria is reset when the Start/Stop button is selected. Manual Sample ID - Auto Increment Off Auto increment feature has been turned off.
Page 556 Error Messages ADVIA 2120/2120i Operator’s Guide MPO Deficiency - Alarm The number of consecutive occurrences of this condition has met the criterion specified for an alarm message. The specified alarm criterion is user definable. Select Customize > System Setup > Alarm/Stop Criteria to view or modify settings.
Page 557 ADVIA 2120/2120i Operator’s Guide Error Messages The specified stop criterion is user definable. Select Customize > System Setup > Alarm/Stop Criteria to view or modify settings. The counter associated with the Stop Criteria is reset when the Start/Stop button is selected.
Page 558 Error Messages ADVIA 2120/2120i Operator’s Guide No Help Available for this Message There is no Help available for this message at this time. If you cannot find information in the Operator's Guide, contact your local technical support provider for assistance.
Page 559 ADVIA 2120/2120i Operator’s Guide Error Messages No Type The type of test request sent by the host is not defined in the Test Dictionary. 1. Verify the accuracy of the host test table. 2. Define the test in Test Dictionary.
Page 560 No Working Buffer Initialized This error can occur during the system preparation (initialization) process. Restart the ADVIA 2120/2120i software. If the error recurs, contact your local technical support provider for assistance. If you have any questions or concerns regarding this document, contact your local technical support provider or distributor.
Page 561 ADVIA 2120/2120i Operator’s Guide Error Messages The specified stop criterion is user definable. Select Customize > System Setup > Alarm/Stop Criteria to view or modify settings. The counter associated with the Stop Criteria is reset when the Start/Stop button is selected.
Page 562 Error Messages ADVIA 2120/2120i Operator’s Guide Nucleated Red Blood Cells - Alarm The number of consecutive occurrences of this condition has met the criterion specified for an alarm message. The specified alarm criterion is user definable. Select Customize > System Setup >...
Page 563 3. If the error recurs, select Customize menu, System Setup, Tools Modify, Port Configuration. 4. Verify that the Inst1 device is listed as ADVIA 2120/2120i and correct the Inst 1 device, if necessary. 5. Check the communications port where the problem occurs (COM 1 or MCA Serial board).
Page 564 Exerciser>Indicators>Analyzer Reset. After 1 minute, if the status line does not display Ready to Run and the green ready-to- aspirate light is still off, exit the ADVIA 2120/ 2120i software, turn off the analyzer and then restart the system. a. Select Shut Down ADVIA in the Log On/Off window.
Page 565 ADVIA 2120/2120i Operator’s Guide Error Messages If you have any questions or concerns regarding this document, contact your local technical support provider or distributor. The possible settings for the parameter are: 0 = parameter not active 1 = Bull's Algorithm...
Page 566 Error Messages ADVIA 2120/2120i Operator’s Guide Increase available patient result database space by deleting data from the sample results file. Select Customize > System Setup > Tools Modify > End of Day. NOTE: Deleting files permanently erases data. If you need an electronic copy of the data, back up the files before deleting.
Page 567 ADVIA 2120/2120i Operator’s Guide Error Messages The alarm counter is automatically reset after reagents are replenished and the Reagent Installation window is updated. . 1. Replenish regents. 2. Select Logs, Reagent Log > Reagent Installation. PEROX 2 Reagent Empty - Stop The number of consecutive occurrences of this condition has met the criterion specified for a stop message.
Page 568 Error Messages ADVIA 2120/2120i Operator’s Guide PEROX 3 Reagent Empty - Stop The number of consecutive occurrences of this condition has met the criterion specified for a stop message. The reagent is empty, and the Autosampler has halted. The specified alarm criterion is user definable in the Customize Menu, System Setup, Alarm/Stop Criteria, Reagent Conditions.
Page 569 ADVIA 2120/2120i Operator’s Guide Error Messages PEROX Irregular Flow Rate - Stop The number of cycles specified for a stop message. The specified stop criterion is user definable in the Customize Menu, System Setup, Alarm/ Stop Criteria. The counter associated with the Stop Criteria is reset when the Start/Stop button is selected.
Page 570 2. After about 1 minute, if the status line does not display Ready to Run and the green ready-to-aspirate light is still off, exit the ADVIA 2120/2120i software, turn off the analyzer and then restart. a. Select Shut Down ADVIA at the Log On/ Off window.
Page 571 2. After about 1 minute, if the status line does not display Ready to Run and the green ready-to-aspirate light is still off, exit the ADVIA 2120/2120i software, turn off the analyzer and then restart. a. Select Shut Down ADVIA at the Log On/Off window.
Page 572 Error Messages ADVIA 2120/2120i Operator’s Guide PEROX SHEATH Reagent Empty - Stop The number of consecutive occurrences of this condition has met the criterion specified for a stop message. The reagent is empty, and the Autosampler has halted. The specified alarm criterion is user definable in the Customize Menu, System Setup, Alarm/Stop Criteria, Reagent Conditions.
Page 573 ADVIA 2120/2120i Operator’s Guide Error Messages PEROX Temperature Out - Stop The number of consecutive occurrences of this condition has met the criterion specified for a stop message. The Autosampler halts at the same time the stop message occurs. The specified stop criterion is user definable in the Customize Menu, System Setup, Alarm/Stop Criteria.
Page 574 Error Messages ADVIA 2120/2120i Operator’s Guide Possible Blasts or Abnormal Cells - Alarm The number of consecutive occurrences of this condition has met the criterion specified for an alarm message. The specified alarm criterion is user definable in the Customize Menu, System Setup, Alarm/Stop Criteria.
Page 575 2. After about 1 minute, if the status line does not display Ready to Run and the green ready-to-aspirate light is still off, exit the ADVIA 2120/2120i software, turn off the analyzer, and then restart. a. Select Shut Down ADVIA at the Log On/Off window.
Page 576 Error Messages ADVIA 2120/2120i Operator’s Guide Possible Cause Corrective Action Pressure out of range. Verify pressure readings and adjust if necessary. Faulty power supply, Contact local technical support provider your fuse, cable, Pressure/ for assistance. Switch Node Board, or If you have any questions or concerns...
Page 577 ADVIA 2120/2120i Operator’s Guide Error Messages Corrective Action Verify printer connections and operation. Typical printer problems include: • Paper jams • Out of paper • Printer offline • Printer disconnected Printer Problem - Stop A printer problem has occurred. The number of consecutive occurrences of this condition has met the criterion specified for a stop message.
Page 578 Error Messages ADVIA 2120/2120i Operator’s Guide Possible Cause Corrective Action Bent or damaged needle Replace the needle. Follow the steps in Replacing the Sampler Needles Faulty Conductivity Contact your local technical support provider Detector for assistance. If you have any questions or concerns regarding this document, contact your local technical support provider or distributor.
Page 579 ADVIA 2120/2120i Operator’s Guide Error Messages RBC Fragments - Alarm The number of consecutive occurrences of this condition has met the criterion specified for an alarm message. The specified alarm criterion is user definable in the Customize Menu, System Setup, Alarm/Stop Criteria.
Page 580 Error Messages ADVIA 2120/2120i Operator’s Guide RBC Irregular Flow Rate - Stop The number of consecutive occurrences of this condition has met the criterion specified for a stop message. The Autosampler halts at the same time the stop message occurs. The specified stop criterion is user definable in the Customize Menu, System Setup, Alarm/Stop Criteria.
Page 581 2. After about 1 minute, if the status line does not display Ready to Run and the green ready-to-aspirate light is still off, exit the ADVIA 2120/2120i software, turn off the analyzer, and then restart. a. Select Shut Down ADVIA at the Log On/Off window.
Page 582: Ready To Run
2. After about 1 minute, if the status line does not display Ready to Run and the green ready-to-aspirate light is still off, exit the ADVIA 2120/2120i software, turn off the analyzer, and then restart. a. Select Shut Down ADVIA at the Log On/Off window.
Page 583 ADVIA 2120/2120i Operator’s Guide Error Messages RETIC Absorption Distribution Abnormal - Alarm The number of consecutive occurrences of this condition has met the criterion specified for an alarm message. The specified alarm criterion is user definable in the Customize Menu, System Setup, Alarm/Stop Criteria.
Page 584 Error Messages ADVIA 2120/2120i Operator’s Guide RETIC Noise Origin - Alarm The number of consecutive occurrences of this condition has met the criterion specified for an alarm message. The specified alarm criterion is user definable in the Customize Menu, System Setup, Alarm/Stop Criteria.
Page 585 ADVIA 2120/2120i Operator’s Guide Error Messages RETIC RBC Count Low - Stop The number of consecutive occurrences of this condition has met the criterion specified for a stop message. The Autosampler halts at the same time the stop message occurs. The specified stop criterion is user definable in the Customize Menu, System Setup, Alarm/Stop Criteria.
Page 586 2. After about 1 minute, if the status line does not display Ready to Run and the green ready-to-aspirate light is still off, exit the ADVIA 2120/2120i software, turn off the analyzer, and then restart. a. Select Shut Down ADVIA at the Log On/Off window.
Page 587 ADVIA 2120/2120i Operator’s Guide Error Messages Sample ID Not Read - Stop Sample-tube barcode label is unreadable or missing. The number of consecutive occurrences of this condition has met the criterion specified for a stop message, and the Autosampler has halted.
Page 588 Error Messages ADVIA 2120/2120i Operator’s Guide Sample SID Not Found - Printing Aborted The sample displayed in the Review and Edit window does not exist in the database. The system cannot provide the requested printout. Corrective Action Correct the problem using the Key list in the Format window.
Page 589 2. After about 1 minute, if the status line does not display Ready to Run and the green ready-to-aspirate light is still off, exit the ADVIA 2120/2120i software, turn off the analyzer, and then restart. a. Select Shut Down ADVIA at the Log On/Off window.
Page 590 2. After about 1 minute, if the status line does not display Ready to Run and the green ready-to-aspirate light is still off, exit the ADVIA 2120/2120i software, turn off the analyzer, and then restart. a. Select Shut Down ADVIA at the Log On/Off window.
Page 591 ADVIA 2120/2120i Operator’s Guide Error Messages SHEATH / RINSE Reagent Container Empty SHEATH/RINSE level has dropped below the level sensor in the SHEATH/ RINSE container, indicating that the container is empty. When this error message is displayed, the aspirate plate will not function, nor will the Ready light illuminate.
Page 592 Error Messages ADVIA 2120/2120i Operator’s Guide Corrective Action If the analyzer is locked in the standby state, verify that the pressure and vacuum readings are within range and adjust if necessary. Select Standby to exit. Status BTRIEVE - Alarm Not Defined An alarm sent from the analyzer is unknown to the Data Manager (not defined in the Alarm Dictionary).
Page 593 System Preparation in Progress When the ADVIA 2120/2120i is first powered on or the software is restarted, the analyzer and the computer go through a preparation process which includes the following: •...
Page 594 The problem may be an acknowledgement problem at the host level. The communication line should be checked by your local Siemens Healthineers Customer Care Center or your local Siemens Healthineers technical support representative for assistance. Type Not Found The type of workorder sent by the host is unknown to the Data Manager.
Page 595 ADVIA 2120/2120i Operator’s Guide Error Messages UFC Vacuum Out of Range UFC Vacuum is out of range. The difference between the compressor vacuum and UFC vacuum is greater than 5” Hg. Possible Cause Corrective Action Vacuum Setting is out of...
Page 596 Error Messages ADVIA 2120/2120i Operator’s Guide Possible Cause Corrective Action Vacuum pump filter Replace the vacuum pump filter. (vacushield) is clogged Crimped waste line Check for a crimped waste line on the waste container. Straighten or reconnect lines as necessary.
Page 597 ADVIA 2120/2120i Operator’s Guide Error Messages Unknown Host Test Number xx A workorder from the host contains a test that is not defined in the Test Dictionary or contains an incorrect host number. Modify the Test Dictionary or correct the host test table.
Page 598 Ready to Run and the green ready-to-aspirate light is still off, follow these steps to exit the ADVIA 2120/2120i software, turn off the analyzer and then restart. a. Select Shut Down ADVIA at the Log On/Off window.
Page 599 2. After about 1 minute, if the status line does not display Ready to Run and the green ready-to-aspirate light is still off, follow these steps to exit the ADVIA 2120/ 2120i software, turn off the analyzer and then restart.
Page 600 Error Messages ADVIA 2120/2120i Operator’s Guide Waste level in the container has reached the level sensor indicating that the container is full. When this error message displays, the aspirate plate will not function, nor will the Ready light illuminate. Possible Cause...
Page 601 The system completes the following steps for the basophil/lobularity cytochemical reactions: 1. ADVIA 2120/2120i BASO reagent lyses the red blood cells and platelets. 2. ADVIA 2120/2120i BASO reagent and the increased temperature in the reaction chamber strips all white blood cells except basophils of their cytoplasm.
Page 602 • The nuclear configuration, which is a combination of the nuclear shape and cell density ADVIA 2120/2120i SHEATH/RINSE encases the sample stream as the two fluids pass through the flowcell. Photodiodes convert the optical signals from the flowcell to electrical pulses.
Page 603 ADVIA 2120/2120i Operator’s Guide Methods • BASO cytogram is formed from the paired low and high-angle light scatter data. Cluster analysis identifies the individual populations. If cluster analysis cannot be used for a particular sample, the cell populations are identified using histogram analysis.
Page 604 Methods ADVIA 2120/2120i Operator’s Guide • The Lobularity Index (LI) value provides an indication of sample abnormality. The Ll is the PMN peak channel number on the Baso X histogram divided by 14, and the PMN peak channel number is greater than 1.9 for normal samples.
Page 605 ADVIA 2120/2120i Operator’s Guide Methods The following BASO cytograms were obtained from a representative patient specimen. Noise Baso Suspect Blast cell nuclei Saturation Mononuclear WBCs Polymorphonuclear WBCs (Monocyte and (Neutrophil and Lymphocyte nuclei) Eosinophil nuclei) Basophils Noise Area This area contains platelets, RBC stroma, and other cellular debris that are not included in the total white blood cell count (WBCB).
Page 606 Methods ADVIA 2120/2120i Operator’s Guide Baso Suspect Cluster Contains unlysed cells other than basophils. Counts from this area are not included in the basophil count. Saturation Area Typically, this area contains air bubbles that are not included in the basophil or WBCB count.
Page 607 ADVIA 2120/2120i Operator’s Guide Methods Baso X histogram A Baso Noise threshold Blast cell Blast threshold Mononuclear WBCs (Monocyte and Lymphocyte nuclei) MN/PMN valley threshold 4 Basophils D Basophil threshold Saturation Baso Saturation Polymorphonuclear WBCs threshold (neutrophil and Eosinophil) Noise nuclei...
Page 608 Methods ADVIA 2120/2120i Operator’s Guide Baso Noise Threshold This threshold (shown as a red line) is set at channel 4 on the y axis. Events below this threshold are counted as noise, while cells above the threshold are included in the white cell count (WBCB).
Page 609 ADVIA 2120/2120i Operator’s Guide Methods Baso Saturation Threshold This threshold (shown as a red line) is set at channel 45 on the x axis and channel 21 on the y axis. Events within this area (microbubbles, clumped cells, and unlysed cells) are monitored for flagging purposes.
Page 610 Methods ADVIA 2120/2120i Operator’s Guide MN/PMN Threshold The “valley“ threshold (shown as a red line) separates the Mononuclear (MN) and Polymorphonuclear (PMN) populations. The valley threshold is a floating threshold that is set using the Baso X histogram. Abnormal Cell Locations If abnormal cells are present, their size and nuclear configuration determine the location of their signals on the BASO cytogram.
Page 611 ADVIA 2120/2120i Operator’s Guide Methods Location of Atypical Lymphocytes on the BASO Cytogram Atypical Lymphocytes appear within the mononuclear (MN) area on the BASO cytogram. Mononuclear area Location of Immature Granulocytes on the BASO Cytogram Immature Granulocytes appear within the MN area on the BASO cytogram.
Page 612 Methods ADVIA 2120/2120i Operator’s Guide Location of NRBCs on the BASO Cytogram Nucleated Red Blood Cells (NRBCs) appear within the PMN population on the BASO cytogram. However, NRBCs are identified for flagging purposes in the peroxidase method. The system uses data from the perox channel and the baso channel to derive the Nucleated Red Blood Cell (NRBC) count (%NRBC and #NRBC).
Page 613: Additional Parameters
ADVIA 2120/2120i Operator’s Guide Methods The following parameters are available for patient reporting: Parameter Explanation WBCB RawWBC x (BasoCalFactor ÷ [1-FracDT]) %BASO 100 x (BASO Count ÷ BASO PHA Cells) #BASO (%BASO ÷ 100) x WBCB The system uses data from the perox channel and the baso channel to derive Nucleated Blood Cells (NRBC) count (%NRBC and #NRBC).
Page 614 Methods ADVIA 2120/2120i Operator’s Guide White blood cell count from the Basophil/Lobularity method # BASO Absolute count of Basophils % BASO Percent of Basophils % BASO Suspect Percent of events from Baso Suspect area % MN Percent of events from Mononuclear area...
Page 615 ADVIA 2120/2120i Operator’s Guide Methods BASO % Dead Time Percent of analysis time when the channel is busy and cannot detect flowcell events BASO % Noise Percent of events from Noise area BASO % Saturation Percent of events from Saturation area...
Page 616 Methods ADVIA 2120/2120i Operator’s Guide BASO Count The number of cells in the Baso area of the BASO cytogram. Baso area Blasts The number of events in the Blasts area of the BASO cytogram. Blasts area 10708774 Rev.04...
Page 617 ADVIA 2120/2120i Operator’s Guide Methods The number of events in the PMN area of the BASO cytogram. PMN area The number of events in the MN area of the BASO cytogram. MN area 10708774 Rev.04...
Page 618 Methods ADVIA 2120/2120i Operator’s Guide Noise The number of events in the Noise area of the BASO cytogram. Noise area BASO Suspect The number of events in the Baso Suspect area of the BASO cytogram. Baso Suspect area RawWBC BASO Valid Cells x (BASO PHA Cells ÷ BASO PHA Total)
Page 619 CSF Samples by counting and distinguishing certain cell types. When using the ADVIA 2120/2120i CSF Assay, the CSF sample is mixed with ADVIA 2120/2120i CSF Reagent, which spheres and fixes the cells. After a minimum 4-minute to 4-hour incubation period, the prepared sample is then aspirated directly into the ADVIA 2120/2120i system.
Page 620 Methods ADVIA 2120/2120i Operator’s Guide CSF Scatter/Absorption Cytogram The CSF Scatter/Absorption Cytogram is a 100 x 100-channel, two- dimensional, pulse height distribution of the scatter high and absorption data for WBC, RBC, and noise, where the X-axis is absorption and the Y-axis is high angle scatter.
Page 621 ADVIA 2120/2120i Operator’s Guide Methods Parameter Explanation CSF RBC CSF RBC/µL = (CSF R Count / CSF PHA Total x CSF PHA Cells) / (1-Fractional Dead Time) x Perox Nominal Factor Additional Parameters The following parameters are for research or laboratory use only and not...
Page 622 Methods ADVIA 2120/2120i Operator’s Guide Parameter Explanation CSF PHA Neuts The number of neutrophils falling in the Neutrophil Region of the CSF Scatter/Scatter cytogram. Due to their scatter/scatter properties, both neutrophils and eosinophils fall in this region of the CSF Scatter/ Scatter cytogram.
Page 623 ADVIA 2120/2120i Operator’s Guide Methods %CSF Mono The reportable CSF differential percentage monocyte cell count. CSF RBC The reportable CSF red blood cell count (RBC/µL). CSF WBC The reportable CSF white blood cell count (WBC/µL). # CSF PMN The reportable CSF absolute polymorphonuclear cell count.
Page 624: Chemical Reactions
The sample and the ADVIA 2120/2120i CN-FREE HGB reagent or ADVIA 2120/2120i HGB reagent are mixed in the hemoglobin reaction chamber (colorimeter). The hemoglobin chemical reactions consist of two...
Page 625 Published studies show that each ferriheme combines with two cyanide ions. The mechanism and clinical performance of the Siemens Hemoglobin method have been described. The presence of carboxyhemoglobin (even up to 100%) has no effect on the reaction rate or transformation to the cyanated product.
Page 626 The hemoglobin transmission histogram is divided into five parts: ADVIA 2120/2120i SHEATH/RINSE reading from previous cycle. Draining of the ADVIA 2120/2120i SHEATH/RINSE, and refilling with the reaction solution consisting of sample and ADVIA 2120/ 2120i HGB reagent Reaction solution readings (15.5s to 18.0s). Sample Mean...
Page 627 ADVIA 2120/2120i Operator’s Guide Methods Calculating Parameters Reported Parameters The following parameters are for patient reporting: Parameter Explanation log (Sample Mean Baseline Mean) x Hgb Cal Factor x 50.0 (HGB RBC) x 10 MCHC (HGB [RBC x MCV]) x 1000...
Page 628 Cytochemical Reactions The peroxidase cytochemical reactions consist of three steps: 1. Red blood cells are lysed using ADVIA 2120/2120i PEROX 1 reagent and the high temperature in the reaction chamber. 2. White blood cells are fixed using ADVIA 2120/2120i PEROX 1 reagent.
Page 629 NOTE: For more detailed information on the contents of ADVIA 2120/2120i PEROX 1 reagent, see Chapter 8. ADVIA 2120/2120i PEROX 1 is the first reagent added to whole blood sample in the heated peroxidase reaction chamber. Its function is to lyse the red blood cells and fix the white blood cells.
Page 630 NOTE: For more detailed information on the contents of ADVIA 2120/2120i PEROX 1 reagent, see Chapter 8. ADVIA 2120/2120i PEROX 2 and ADVIA 2120/2120i PEROX 3 are added to the peroxidase reaction chamber in the second reagent addition. The 4-Chloro-1-naphthol in ADVIA 2120/2120i PEROX 2 serves as a...
Page 631 ADVIA 2120/2120i Operator’s Guide Methods PEROX Y histogram displays the light scattering values for all events, including those from the noise area. Noise-Lymph histogram provides the light scattering values for the noise, lymphocyte, and large unstained cells (LUC) areas. Information is excluded from the monocyte, neutrophil, and eosinophil areas.
Page 632 Methods ADVIA 2120/2120i Operator’s Guide Perox Y Histogram The PEROX Y histogram displays the light scattering values for all events, including those from the noise area. Noise Lymphocytes Large Unstained Cells (LUC) Increasing channel numbers on the PEROX Y histogram correspond to greater cell size (volume).
Page 633 ADVIA 2120/2120i Operator’s Guide Methods Mode channel Peak channel Valley channel PEROX Cytogram The PEROX cytogram is divided into 100 counting channels on each axis. The cells absorb light proportional to the amount of peroxidase stain present, and this is represented on the y axis.
Page 634 Methods ADVIA 2120/2120i Operator’s Guide The following PEROX cytograms were obtained from a representative patient specimen. Noise Large Unstained Cells Nucleated Red Blood Monocytes Cells Platelet Clump Neutrophils Lymphocytes and Eosinophils Basophils Noise This area contains Platelets, RBC stroma, and debris that are excluded from the Total White Count (WBCP)and WBC differential.
Page 635 ADVIA 2120/2120i Operator’s Guide Methods Lymphocyte and Basophil Cluster Lymphocytes and Basophils display in the same cluster because they have similar size and no peroxidase activity. LUC Cluster The LUC cluster contains Large Unstained Cells (atypical lymphocytes, blasts, and other abnormal cells.) Monocyte Cluster Monocytes are medium-sized cells with some peroxidase activity.
Page 636 Methods ADVIA 2120/2120i Operator’s Guide Abnormal Cell Locations If abnormal cells are present, their size and peroxidase activity determine their location on the PEROX cytograms. nRBCs Location on the Perox Cytogram Nucleated Red Blood Cells (nRBCs) appear in the NRBC cluster, which is located between the Noise and Lymphocyte areas.
Page 637 ADVIA 2120/2120i Operator’s Guide Methods Atypical Lymphocytes Location on the PEROX Cytogram Atypical lymphocytes are large cells with no peroxidase activity that can appear in the LUC area. LUC area Blasts Location on the PEROX Cytogram Similarly, blasts and other large abnormal cells with no peroxidase activity also appear in the LUC area.
Page 638 Methods ADVIA 2120/2120i Operator’s Guide Immature Granulocytes Location on the PEROX Cytogram Immature Granulocytes are large cells with high peroxidase activity that can appear in the neutrophil area. Neutrophil area Bands Location on PEROX the Cytogram Bands display within the Neutrophil population due to their similar size and degree of peroxidase staining.
Page 639 ADVIA 2120/2120i Operator’s Guide Methods Platelet Clumps Location on the PEROX Cytogram Platelet Clumps form a separate cluster that originates in the Noise area on the PEROX cytogram. Separate cluster Unlysed RBCs Location on PEROX Cytogram Unlysed RBCs with no peroxidase activity appear along the left side of the PEROX cytogram.
Page 640: Additional Parameters
Methods ADVIA 2120/2120i Operator’s Guide Calculating Parameters Reported Parameters Parameter Explanation WBCP RawWBC x (PeroxCalFactor ÷ [1-FracDT]) %NEUT (100 x Neutrophil Count) ÷ PHA Cells #NEUT (%NEUT ÷ 100) x WBC %LYMPH ([100 x Lymphocyte Count] ÷ PHA Cells) - %BASO #LYMPH (%LYMPH ÷...
Page 641 ADVIA 2120/2120i Operator’s Guide Methods MPXI X Mean of Sample Neut - Expected staining index * 100 Expected staining index NEUT X Mean X channel for neutrophil cluster NEUT Y Mean Y channel for neutrophil cluster Noise/Lymph Valley Valley channel between Noise and Lymph...
Page 642 Methods ADVIA 2120/2120i Operator’s Guide Neutrophil Count The number of events in the Neutrophil area of the PEROX cytogram. Neutrophil area %LYMPH The percentage of lymphocytes. #LYMPH The absolute count of lymphocytes. Lymphocyte Count The number of events in the Lymphocyte area of the PEROX cytogram.
Page 643 ADVIA 2120/2120i Operator’s Guide Methods %MONO The percentage of monocytes. #MONO The absolute count of monocytes. Monocyte Count The number of events in the Monocyte area of the PEROX cytogram. Monocyte area %EOS The percentage of eosinophils. #EOS The absolute count of eosinophils.
Page 644 Methods ADVIA 2120/2120i Operator’s Guide Eosinophil Count The number of events in the Eosinophil area of the PEROX cytogram. Eosinophil area %LUC The percentage of large unstained cells. #LUC The absolute count of large unstained cells. Large Unstained Cell Count The number of events in the the PEROX cytogram LUC area.
Page 645 ADVIA 2120/2120i Operator’s Guide Methods PeroxCalFactor View the PEROX WBC calibration factors, by selecting System Logs>Cal/Gain Logs. Fractional Down Time The fraction of time that the channel is busy processing flowcell events. While the perox channel is busy identifying a particular flowcell event, the perox channel is unable to process any additional events that might occur (dead time).
Page 646 Methods ADVIA 2120/2120i Operator’s Guide Clumps Count The number of events in the PEROX cytogram Platelet Clumps area. Platelet Clumps area Valley Count The number of events in the PEROX cytogram NRBC area. NRBC area 10708774 Rev.04...
Page 647 A constant volume of the cell suspension from the RBC reaction chamber passes through the flowcell where the low-angle light scatter (2°–3°) and high-angle light scatter (5°–15°) signatures of each cell are measured. ADVIA 2120/2120i SHEATH/RINSE encases the sample stream as the two fluids pass through the flowcell. 10708774 Rev.04...
Page 648 Methods ADVIA 2120/2120i Operator’s Guide The two light-scatter signals are detected, electronically amplified, and split into four signals: • A pair of low-angle light scatter (2°–3°) and high-angle light scatter (5° – 15°) signals are used to analyze red blood cells.
Page 649 ADVIA 2120/2120i Operator’s Guide Methods Using the Mie theory of light scattering for homogeneous spheres, the low- angle, high-gain light scatter measurement is converted into cell volume and the high-angle, high-gain light scatter measurement is converted into refractive index (n).
Page 650 Methods ADVIA 2120/2120i Operator’s Guide RBC Volume Histogram The RBC Volume histogram represents the red blood cells distribution by cell volume. The histogram has a range of 0–200 fL. RBC Volume histogram (normal sample) Normal samples have a bell-curve shaped distribution with a mode channel between 60–20 fL.
Page 651 ADVIA 2120/2120i Operator’s Guide Methods RBC Volume histogram (microcytic sample) In samples with increased numbers of microcytic red blood cells, the histogram curve shifts to the left, indicating an increase in the percentage of the cells with volumes that are less than 60 fL.
Page 652 Methods ADVIA 2120/2120i Operator’s Guide RBC Volume histogram (anisocytosis) RDW is monitored as an indication of anisocytosis, and the results are flagged if the RDW exceeds 16%. Note that two specimens with the same RDW value can have different degrees of microcytosis and macrocytosis.
Page 653 ADVIA 2120/2120i Operator’s Guide Methods RBC HC Histogram (normal sample) Normal samples have a bell-curve shaped Hgb concentration distribution with a mean channel between 28–41 g/dL. The cell hemoglobin concentration mean (CHCM) and the hemoglobin distribution width (HDW) are obtained from this histogram. The CHCM is the mean of the RBC HC histogram.
Page 654 Methods ADVIA 2120/2120i Operator’s Guide RBC HC Histogram (hypechromic sample) In samples with increased numbers of hyperchromic RBCs, the histogram curve shifts to the right, indicating an increase in the percentage of the cells with hemoglobin concentrations greater than 41 g/dL.
Page 655 ADVIA 2120/2120i Operator’s Guide Methods RBC CH Histogram The RBC CH (cellular hemoglobin) histogram represents the red blood cells distribution by the amount of hemoglobin that is present in each cell, independent of the cell volume. The histogram has a range of 0–100 picograms.
Page 656 Methods ADVIA 2120/2120i Operator’s Guide Using the Mie theory of light scattering for homogeneous spheres, the low- angle and high-angle light scatter signals for each cell are transformed into the volume and hemoglobin concentration values. The RBC map shows the relationship between the light-scatter measurements and the volume and hemoglobin concentration cell-by-cell characteristics.
Page 657 ADVIA 2120/2120i Operator’s Guide Methods On the y-axis, RBC volume markers are set at 60 fL and 120 fL. Red blood cells with a volume less than 60 fL are microcytic, while cells with a volume greater than 120 fL are macrocytic.
Page 658 Methods ADVIA 2120/2120i Operator’s Guide Platelet Y Histogram The Platelet Y histogram is a 100–channel display of the low-angle (2° – 3°), high-gain light scatter measurements that corresponds to the y–axis on the PLT Scatter cytogram. Platelet VOL Histogram The Platelet VOL histogram of the two-dimensional PLT analysis shows the cells distribution by volume.
Page 659 ADVIA 2120/2120i Operator’s Guide Methods Platelet PC Histogram The Platelet PC histogram of the two-dimensional PLT analysis shows the platelets distribution according to the refractive index (platelet component concentration [PC]). The histogram has a range of 0–5.0 pg. PLT Scatter Cytogram The PLT Scatter cytogram is the graphical representation of two light- scatter measurements: the high-angle (5°–15°), high-gain light scatter is...
Page 660 Methods ADVIA 2120/2120i Operator’s Guide Using the Mie theory of light scattering for homogeneous spheres, the low- angle and high-angle light scatter signals for each cell are transformed into volume and refractive index (n) values. The PLT map shows the relationship between the light-scatter measurements and the cell-by-cell characteristics of volume and refractive index.
Page 661 ADVIA 2120/2120i Operator’s Guide Methods PLT Volume / PC Cytogram The Platelet Volume / Refractive Index (PC) cytogram is a 100 by 100 channel display. Platelets Red blood cells A x-axis refractive index (concentration of the platelet component [PC]) range: 0–40 g/dL y-axis platelet volume range: 0–69fL...
Page 662: Additional Parameters
Methods ADVIA 2120/2120i Operator’s Guide The Platelet VOL histogram contains platelets and large platelets with volumes up to 60 fL. Calculating RBC Parameters Reported Parameters Parameter Explanation Number of Red Cells x RBC Cal Factor x Dilution Factor x Coincidence-correction Factor Mean of RBC Volume histogram 100 x (SD of RBC Volume histogram ÷...
Page 663 ADVIA 2120/2120i Operator’s Guide Methods Parameter Explanation RBC % Dead Time 100 x (Measured Dead Time ÷ Measured Sample Time) RBC Coin Level Number of coincidence events to be trimmed RBC Coin Count Coincidence Count in RBC Scatter cytogram RBC R Count...
Page 664 Methods ADVIA 2120/2120i Operator’s Guide Cellular Hemoglobin Content Hemoglobin Concentration Distribution Width Hematocrit Number of red cells (RBC R Count x RBC Valid Cells) ÷ (RBC R Count + RBC P Count) RBC R Count Number of cells in the RBC area of the RBC Scatter Cytogram.
Page 665 ADVIA 2120/2120i Operator’s Guide Methods RBC P Count The number of cells in the platelet area of the RBC Scatter Cytogram. Platelet area CHDW Cell Hemoglobin Distribution Width RBC % Dead Time Percent of analysis time when the channel is busy and cannot detect...
Page 666 Methods ADVIA 2120/2120i Operator’s Guide # MICRO Number of microcytic red blood cells % HYPER Percent of hyperchromic red blood cells % HYPO Percent of hypochromic red blood cells %Micro / %Hypo Ratio This parameter is reported to be of use in differentiating two types of microcytic anemia.
Page 667 ADVIA 2120/2120i Operator’s Guide Methods Parameter Explanation PLT N Mean of refractive index values for platelets only PLT X Mean of high-angle, high-gain values for platelets only PLT Y Mean of low-angle, high-gain values for platelets only PMDW SD of Platelet PM histogram...
Page 668 Methods ADVIA 2120/2120i Operator’s Guide RBC Fragments This count includes events in the RBC Fragment area of the PLT Scatter cytogram that have volumes less than 30fL and refractive indexes greater than 1.400. RBC Ghost area Platelet area RBC Fragment area The RBC Fragments Count is in the same units selected for RBC on the Unit Set Configuration window of the System Setup tab.
Page 669 ADVIA 2120/2120i Operator’s Guide Methods RBC Ghosts This count includes events in the RBC Ghost area of the PLT Scatter cytogram that have refractive indexes lower than 1.350. RBC Ghost area Platelet area RBC Fragment area The RBC Ghosts Count is in the same units selected for RBC on the Unit Set Configuration window of the System Setup tab.
Page 670 (R Count-2D x RBC Valid Cells) ÷ (R Count-2D + P Count-2D) NRBC Analysis NRBC Method Description The ADVIA 2120/2120i NRBC analysis method reports NRBC counts for whole blood samples with either 200 or more nRBC/µL or with at least 2% nRBCs with a WBC count of at least 3000/µL.
Page 671 ADVIA 2120/2120i Operator’s Guide Methods PEROX Cytogram Baso Cytogram with location of nRBC and with location of nRBC Unstained line The method identifies nRBCs by nuclear size in the Peroxidase channel, and by nuclear density in the Basophil/Lobularity channel. In the unstained region of the Peroxidase Channel cytogram, nRBC nuclei are located between the noise and lymphocytes.
Page 672 Methods ADVIA 2120/2120i Operator’s Guide Residual count The NRBC count from subtracting the noise and lymphocytes from the unstained region of the Peroxidase Channel Y-axis histogram Barox count The NRBC count from the equation #nRBC = #PMNs - #NEUTs - #EOS The system then uses selection rules to determine which, if any, of the NRBC counts to report.
Page 673 NOTE: For more detailed information on the contents of the ADVIA 2120/2120i autoRETIC reagent, see Chapter 8. The ADVIA 2120/2120i autoRETIC reagent contains a zwitterionic detergent (surfactant) that isovolumetrically spheres the red cells. It also contains a cationic dye, Oxazine 750, that stains cells according to their RNA content.
Page 674 (2°–3°), the high-angle light scatter (5°–15°), and the absorption signatures for each cell are measured. ADVIA 2120/2120i SHEATH/RINSE encases the sample stream as the two fluids pass through the flowcell. The low-angle and high-angle light scatter signatures are proportional to the cell size and hemoglobin concentration.
Page 675 “Gated“ refers to the total population of RBCs containing both mature RBCs and reticulocytes. Negative versus Positive Cell Populations Since reticulocytes have RNA and stain with ADVIA 2120/2120i autoRETIC reagent, they are the positive cell population. Mature RBCs do not stain, and they are the negative cell population.
Page 676 Methods ADVIA 2120/2120i Operator’s Guide RETIC Abs Flatness Histogram The RETIC Abs Flatness histogram data consists of 50 points, one taken every 200 milliseconds. Each point represents the mean absorption for the last 200 milliseconds. This histogram provides a visual indication of the absorption signal “flatness“...
Page 677 ADVIA 2120/2120i Operator’s Guide Methods RETIC Volume Histogram The RETIC Volume histogram represents the overlaid distributions of mature RBCs and reticulocytes by cell size only. The histogram has a range from 0 fL to 200 fL. The displayed data includes the MCVg calibration factor.
Page 678 Methods ADVIA 2120/2120i Operator’s Guide RDWm Red cell Distribution Width is the coefficient of variation (CV) of the RETIC Volume histogram (channels 15 to 99) for the mature RBC population. RDW Delta RDWr - RDWm 10708774 Rev.04...
Page 679 ADVIA 2120/2120i Operator’s Guide Methods RETIC HC Histogram The RETIC hemoglobin concentration (RETIC HC) histogram represents the overlaid distributions of mature RBCs and reticulocytes by cellular hemoglobin concentration only. The histogram has a range from 0–50 g/dL. The data includes the CHCMg calibration factor.
Page 680 Methods ADVIA 2120/2120i Operator’s Guide HDWr Hemoglobin Distribution Width is the standard deviation (SD) of the RETIC HC histogram for the reticulocyte population. HDWm Hemoglobin Distribution Width is the standard deviation (SD) of the RETIC HC histogram for the mature RBC population.
Page 681 ADVIA 2120/2120i Operator’s Guide Methods RETIC CH Histogram The RETIC cellular hemoglobin (RETIC CH) histogram represents the overlaid distributions of mature RBCs and reticulocytes by the actual weight or mass of hemoglobin present in each cell. The histogram has a range from 0 pg to 100 pg.
Page 682 Methods ADVIA 2120/2120i Operator’s Guide CHDWm Cellular Hemoglobin Distribution Width is the standard deviation (SD) of the RETIC CH histogram for the mature RBC population. CHDWg Cellular Hemoglobin Distribution Width is the standard deviation (SD) of the RETIC CH histogram for the gated cells.
Page 683 ADVIA 2120/2120i Operator’s Guide Methods RETIC Scatter Abs Cytogram The RETIC Scatter ABS cytogram is the graphical representation of the absorption and light-scatter measurements: the high-gain, absorption (cell maturation) is plotted along the x axis and the high-angle, low-gain light scatter (cell size) is plotted along the y axis.
Page 684 Methods ADVIA 2120/2120i Operator’s Guide The RTC threshold (3) on the x axis is set as follows: 1. The analyzer scans for the absorption mode between channels 2 and 25 on the x axis. 2. The standard deviation (SD) of the six channels on either side of the mode is calculated.
Page 685 ADVIA 2120/2120i Operator’s Guide Methods RETIC Scatter Cytogram The RETIC Scatter cytogram is the graphic representation of two light- scatter measurements: the high-angle, low-gain light scatter is plotted along the x axis, and the low-angle, low-gain light scatter is plotted along the y axis.
Page 686: System Parameters
Methods ADVIA 2120/2120i Operator’s Guide Calculating Retic Parameters Reported Parameters Parameter Explanation #Retic RBC x (%Retic ÷ 100) x 1000 %Retic 100 x (RETIC Count ÷ #RTC Gated Cells) x % RETIC Cal Factor Mean of the RETIC CH histogram for the reticulocyte population.
Page 687 ADVIA 2120/2120i Operator’s Guide Methods Additional Parameters The following parameters are for research or laboratory use only and are not for patient reporting: Parameter Explanation CHCMr Mean of the Retic HC histogram for the reticulocyte population MCVm Mean of the RETIC Volume histogram for the...
Page 688 Methods ADVIA 2120/2120i Operator’s Guide CHCMr Reticulocyte Cellular Hemoglobin Concentration Mean Reticulocyte Cellular Hemoglobin Content MCVm Mean Cell Volume of mature population MCVr Mean Cell Volume of reticulocyte population The system always uses the RBC count from the RBC / Platelet method, even when a sample has been run with a retic only selectivity.
Page 689 ADVIA 2120/2120i Operator’s Guide Methods RTC Gated Cells The number of cells between the RTC platelet and RTC coincidence thresholds in the Retic Scatter Abs cytogram excluding channel 99. Neg RBC The number of cells in the mature RBC area of the Retic Scatter Abs cytogram.
Page 690 Methods ADVIA 2120/2120i Operator’s Guide Number of Reticulocytes in the gated population The total number of cells in the three reticulocyte areas of the Retic Scatter Abs cytogram. Retic Count Count of reticulocytes in the gated population Number of Low Absorption Reticulocytes The number of gated cells between the RTC and Low/Medium RTC thresholds of the Retic Scatter Abs cytogram.
Page 691 ADVIA 2120/2120i Operator’s Guide Methods %LRetic Percent of low absorption reticulocytes Number of Medium Absorption Reticulocytes The number of gated cells between the Low/Medium RTC and Medium/ High RTC thresholds of the Retic Scatter Abs cytogram. Number of medium reticulocytes...
Page 692 PD fluids. The TNC count is an absolute count of the nucleated blood cells, mesothelial cells, and other non-hematopoietic cells found in these fluids. The ADVIA 2120/2120i Body Fluid Application TNC and RBC counts are used in conjunction with other diagnostic information and the attending healthcare professional’s evaluation of the...
Page 693 TNC and RBC counts. No additional Body Fluid Application specific reagents are needed. Instrument The Body Fluid Application can be run on the ADVIA 2120 and the ADVIA 2120i hematology analyzers. See the ADVIA 2120/2120i Operator’s Guide for detailed instructions on hematology analyzer requirements, calibration procedures and service/ maintenance requirements.
Page 694 Methods ADVIA 2120/2120i Operator’s Guide The Basophil/Lobularity and Peroxidase channel cytograms were modified specifically for the light scatter characteristics of the cells in body fluid samples. The RBC cytogram is the same as the cytogram for whole blood analysis. The reported TNC count derives from the Basophil/Lobularity channel. The TNC count is the sum of the events above the Baso Y-channel Noise threshold and the events left of Baso X-channel 49.
Page 695 ADVIA 2120/2120i Operator’s Guide Methods Manual Sample ID Window The Manual Sample ID window includes the following sections: • Body Fluid Sample button • A selection box with a drop-down arrow • Body Fluid Rinse button When selected, the Body Fluid Sample button places the system in the...
Page 696 RBC counts are not reported and the Body Fluid Application sample/ system flags are not applied. For ADVIA 2120/2120i systems connected to the Autoslide, slides are not made for body fluid samples with workorders. A stained cytocentrifuge preparation is the recommended method for differentiating cells in body fluid samples.
Page 697: Instrument Setup
Instrument Setup This section includes important software information for configuring the Data Manager and the ADVIA 2120/2120i system software for analysis of body fluid samples. NOTE: Back up the current Data Manager settings. Perform a backup by selecting End of Day in Tools Modify. Access Tools Modify from System Setup or Analyzer Setup in the Customize menu.
Page 698 Methods ADVIA 2120/2120i Operator’s Guide 3. Under Characteristics, enter the following information: Units Body Code Type Conventional Test Host Fluid Pleural pITNC PLF cells/µL x 10e6 cells/L Fluid Pleural plRBC PLF x 10e3 cells/ìL x 10e9 cells/L Fluid Peritoneal prTNC PRF cells/µL...
Page 699 ADVIA 2120/2120i Operator’s Guide Methods Body Fluid Configuration Window–Setup The Body Fluid Configuration window must be set up so the system software recognizes and handles the body fluid types configured in the Data Manager. NOTE: The Body Fluid Configuration window is grayed out when the Body Fluid Application TNC and RBC tests were not created in the Data Manager.
Page 700 Methods ADVIA 2120/2120i Operator’s Guide Optional: Table Dictionary–Create Body Fluid Profile The steps in this section are optional when workorders are downloaded from the LIS but are necessary when workorders are created using the Data Manager. Create body fluid Profiles and Types if the laboratory uses Order Entry under the Data Manager: 1.
Page 701 3. Add comments by typing a code in the Code box and the descriptive text in the Name box. Optional: Creating Reports See the ADVIA 2120/2120i Operator’s Guide for more detailed information on editing report format files for the Report.par, Print.par, and Prevalid.par. 1. At the Tools–Modify menu under the Data Manager, double-select Format.
Page 702 Methods ADVIA 2120/2120i Operator’s Guide Optional: Creating an Image Configuration File The Image Configuration file displays the appropriate cytograms, and/or histograms for the Review and Edit function, and display on the Data Manager report printouts. Creation of the Image Configuration file includes placing cytograms and/or histograms in a grid composed of 9 boxes.
Page 703: Background Count
ADVIA 2120/2120i Operator’s Guide Methods Background Count Obtaining background counts by running a clean saline or phosphate buffered saline (PBS) sample as a body fluid sample after selecting the Body Fluid Sample button on the Manual Sample ID screen. Acceptable background counts are: •...
Page 704: Materials Provided
5. Load the rack on the Autosampler and select Start/Stop on the analyzer touchpad. Materials Provided The Body Fluid Method is available for ADVIA 2120/2120i Hematology Systems using version 5.6.6 Software or higher. 10708774 Rev.04...
Page 705: Materials Required But Not Provided
Performance Characteristics Accuracy The Body Fluid Application TNC count was evaluated by comparing the manual chamber count to the ADVIA 2120/2120i count for 434 body fluid samples. The Body Fluid Application RBC count was evaluated by comparing the manual chamber count to the ADVIA 2120/2120i count for 78 body fluid samples.
Page 706 –6.76 x 10 cells/µL. Carryover The ADVIA 2120/2120i Body Fluid Application utilizes a Body Fluid Rinse cycle approximately 40-seconds in duration using the ADVIA 120/2120/2120i Sheath/Rinse reagent to cleanse the hydraulic pathways and reaction chambers for samples run using the MOTS and MCTS.
Page 707 4. If the sample is form a patient under treatment with a sclerosing agent, then confirm the TNC and RBC counts by an alternate method. Sample/System Flags The Body Fluid Application uses a selected group of ADVIA 2120/2120i sample/system flags for identifying samples that can have invalid TNC or RBC counts.
Page 708 Methods ADVIA 2120/2120i Operator’s Guide Table 4: Sample/System Flags Modified for the Body Fluid Application Sample/System Flag Triggering Conditions WBC-CE TNC 100–2000 cells/µL AND WBC Comparison %Perox Noise > 50 and the ABS (WBCB-WBCP) > 40% Error WBCB TNC < 2000 cells/µL AND %Perox Noise <...
Page 709 ADVIA 2120/2120i Operator’s Guide Methods Table 5: Sample/System Flags Not Modified for the Body Fluid Application Sample/System Flag Triggering conditions LAS-PL (PL) The laser light intensity in the RBC/Baso/Retic channel is less than 150. Laser Power Low PX-PL (PX) The light intensity in the Perox channel is less than...
Page 710 227 (peritoneal fluid). The range of expected values for PD specimens, Table 6, was determined on ADVIA 2120 system from 73 PD specimens and confirmed by manual methods to be free of inflammation.The generally accepted reference interval is WBC < 100/µL and < 50% polymorphonuclear neutrophils.
Page 711: Software Limitations
Data Manager code. 5. When ordering body fluid sample tests at the LIS, do not include whole blood tests with body fluid tests in the same workorder as the ADVIA 2120/2120i Data Manager can select the incorrect sample type. 10708774 Rev.04...
Page 712 Methods ADVIA 2120/2120i Operator’s Guide 6. At the Review and Edit screen TNC and RBC results reported as “< 20 cells/µL” or “< 10 x 10 cells/µL” are not highlighted if they are out-of-range based on the Review Range criteria.
Page 713: Sample Collection And Preparation
NOTE: The performance data for the methods were obtained using ADVIA 2120/2120i instruments, software, reagents, standards, and controls designed for or intended for use on the ADVIA 2120/2120i Hematology System. Use the quality control material in accordance with the quality control instructions for use.
Page 714 B or C viruses, HIV, or other infectious agents are absent, these products should be handled according to established good laboratory practices. Siemens supplies control and calibrator products for use with the ADVIA 2120/2120i Hematology System. Refer to the current Price List, and the product package insert for additional information.
Page 715 ADVIA 2120/2120i Operator’s Guide Regulatory ADVIA 120 TESTpoint 3-in 1 Hematology Control Abnormal 1 Symbol Contents Amount (mL) Hematology Control Abnormal 1 4 x 4.0 mL Hematology Control Abnormal 1 4.0 mL ADVIA 120 TEST Point 3-in-1Hematology Control Abnormal 2...
Page 716 Regulatory ADVIA 2120/2120i Operator’s Guide ADVIA 120 TESTpoint Hematology Control High Symbol Contents Amount (mL) Hematology Control High 4 x 4.0 mL Hematology Control High 4.0 mL ADVIA 120 TESTpoint Hematology Control Low Symbol Contents Amount (mL) Hematology Control Low 4 x 4.0 mL...
Page 717 The System Specific Target Values (SSV) are assigned for ADVIA 120 SETpoint Hematology Calibrator and ADVIA 120 TESTpoint Hematology Control products by assaying each lot on an ADVIA 2120/ 2120i Hematology System that was calibrated using fresh, normal, whole- blood samples that were assigned RBC, WBC, Plt, Hct, and Hgb values using the methods described above.
Page 718 OPTIpoint value assignment, the temperature of the effluent is monitored so the correct index of refraction can be used to standardize the ADVIA 2120/2120i system. When stored at 2°C to 8°C, unopened ADVIA OPTIpoint , ADVIA SETpoint Calibrator, and ADVIA TESTpoint Reticu are stable until the last day of the month (expiration date) printed on the product label, unless otherwise stated.
Page 719: Operation
In no event shall Siemens be liable for errors that are introduced by, or result from, any modification or alteration of the computer disks and/or the programs contained on such disks by the user, or for any direct or indirect consequences resulting from such modification or alteration.
Page 720 Siemens products do not make diagnoses on patients. Siemens intends its diagnostic products (systems, reagents, software, hardware) to be used to collect data reflecting the patient's chemical, hematological, or immunological status at a certain point in time.
Page 721 The principal wastes associated with the use of the ADVIA 2120/2120i Hematology system are reagent containers, sample containers, system waste, and customer-replaceable system components. AllADVIA 2120/2120i reagent containers are manufactured of recyclable high-density polyethylene.
Page 722 TOC (g/dL) Cyanide 2.38 0.33 <0.05 Formaldehyde adsorbable organic halide 0.59 1.97 2.32 purgeable organic halide 0.52 1.03 2.52 (mg/dL of Cl) DMF was calculated by Siemens based on the dilution of the autoRETIC reagent in the system waste. 10708774 Rev.04...
Page 723 ADVIA 2120/2120i Operator’s Guide Regulatory Volatile Organic Compounds in System Waste Concentrations are ?g/L (ppb) unless otherwise noted. < indicates that the concentration is below the limit of detection Determinand CBC/Diff CBC/Diff/Retic Dichloromethane <25 <25 <25 Chloroform 1,1,1 - Trichloroethane <5...
Page 724 Regulatory ADVIA 2120/2120i Operator’s Guide Determinand CBC/Diff CBC/Diff/Retic 1,1,2 - Trichloroethane <1 <1 <1 1,1 - Dichloroethene <1 <1 <1 trans 1,2 - Dichloroethene <1 <1 <1 cis 1,2 - Dichloroethene <1 <1 <1 Tert-butyl methyl ether <1 <1 <1 Vinyl chloride <25...
Page 725 Where necessary, appropriate safety equipment should be used (e.g., eye protection, gloves, lab coats). Users should follow the handling and safety precautions specified in the Material Safety Data Sheets available from Siemens. Reagent and Reagents and calibrators are ready-to-use and require Calibrator no preparation.
Page 726 System Method Information: Quality Control This section specifies recommended quality control material and quality control frequency for the individual method. In general, Siemens recommends that ADVIA 2120/2120i Hematology Systems be monitored using the ADVIA TESTpoint Hematology Controls (Low, Normal, and High) and ADVIA TESTpoint Reticulocyte Control (Low and High).
Page 727 Calibration Procedure The system must be calibrated for all parameters except % Retics. Siemens recommends the use of ADVIA SETpoint Calibrator. Calibration should be performed in each of the following cases: •...
Page 728 Regulatory ADVIA 2120/2120i Operator’s Guide Siemens makes every effort to identify in the Troubleshooting, Maintenance, and Bibliography topics of the Product Labeling, the potential effects caused by instruments, reagents, or endogenous substances, including known interferences on patient results. However, it is impossible to provide information that would be applicable to every clinical situation.
Page 729: Intended Use
Some specimens may exceed the range of the method and produce error flags or messages. CBC Method Intended Use The ADVIA 2120/2120i Hematology System Complete Blood Count (CBC) method is intended to quantitatively measure the following hematological parameters: • White Blood Cell count (WBC) •...
Page 730 2 different gain settings. On the ADVIA 2120/2120i Hematology system the platelet signals are amplified considerably more than the RBC signals. Coincidence correction is made to each of the counts so that accurate counts are made over a wide range of each cell type.
Page 731 Blood 68:506-513 (1986) Reagents The following ready-to-use reagents are required to perform the CBC method and maintain the ADVIA 2120/2120i Hematology System. You must use either the CBC TIMEPAC or the CN-FREE CBC TIMEPAC along with the SHEATH/RINSE. 10708774 Rev.04...
Page 732 Regulatory ADVIA 2120/2120i Operator’s Guide ADVIA 120 CBC TIMEPAC Symbol Contents Amount (mL) CBC TIMEPAC DEFOAMER 1 x 75 mL RBC/PLT 2 x 2700 2 x 1100 BASO 2 x 1100 ADVIA 120 CN-FREE CBC TIMEPAC Symbol Contents Amount (mL)
Page 733 ADVIA 2120/2120i Operator’s Guide Regulatory ADVIA 120 SHEATH/RINSE Symbol Contents Amount (mL) SHEATH/RINSE 10 L SHEATH/RINSE 20 L Amount Contains ADVIA 120 DEFOAMER 1 x 75 mL Silicone emulsion, 100% Shake before use. For in vitro diagnostic use. ADVIA 120 RBC / PLT...
Page 734: Storage And Stability
The gain adjustment procedure is used to adjust the amplification signals in a channel to properly position the cell signatures within a cytogram. Calibration The system must be calibrated for all parameters except %RETIC. Siemens recommends the use of the ADVIA SETpoint Calibrator. Calibration procedure should be performed: •...
Page 735 ADVIA 2120/2120i Operator’s Guide Regulatory • Anytime control results and/or moving averages are out of range and has been verified that the out-of-control condition is instrument- related. Sample Handling Sample Collection Collect whole blood in an evacuated blood collection tube containing EDTA as an anticoagulant.
Page 736: Materials Required But Not Provided
Materials Required but not Provided For the ADVIA 2120/2120i Hematology System, the other materials required to perform the ADVIA 2120/2120i CBC method are the various control materials, calibrators, ADVIA OPTIpoint, and other reagents or equipment specified in the Methods Introduction section.
Page 737: Limitations Of The Procedure
ADVIA 2120/2120i Operator’s Guide Regulatory Limitations of the Procedure The limitations of the procedure are as listed: 1. Some irreversibly sickled cells that occur in cases of sickle cell anemia may not completely sphere in the system, resulting in an elevated RDW and therefore an underestimation of the MCV.
Page 738 Regulatory ADVIA 2120/2120i Operator’s Guide The following range of expected values is based upon the duplicate assay of 60 whole-blood specimens obtained from an adult population of assumed healthy individuals. Parameter Room Temperature Refrigerated Temperature Stability (hours) Stability (hours) WBC (10³/µL) RBC (10 /µL)
Page 739 ADVIA 2120/2120i Operator’s Guide Regulatory Parameter Mean Standard Deviation CV (%) PLT (10³/µL) MPV (fL) 0.11 Performance Characteristics: Correlation Data The performance of the ADVIA 120 Hematology System was compared with the performance of the Technicon H•3 RTC System (x) using whole blood from 60 normal donors and 70 hospital donors with each sample run in duplicate (n = 260).
Page 740 0.2% 0.1% Performance Characteristics: Analytical Range The analytical range for the ADVIA 2120/2120i Hematology System was established by making serial dilutions of samples specially prepared to have high analyte values. Multiple assays of the specially prepared pools were performed on the system.
Page 741 ADVIA 2120/2120i Operator’s Guide Regulatory Reagent ADVIA 120 CSF Reagent Symbol Contents Amount (mL) CSF Reagent 1 x 8 mL CSF Reagent 8 mL ADVIA 120 CSF Reagent contains a sphering agent and fixatives in a buffered aqueous solution. The ADVIA 120 CSF Reagent (PN T01-4610-01) contains one 8 mL bottle with sufficient reagent to analyze 25 samples or controls.
Page 742: Sample Handling
Sample Stability CSF patient samples are not stable and should be assayed as soon as possible after collection. Siemens recommends that CSF patient samples be prepared with CSF reagent within one hour after collection. Once prepared with CSF reagent, the prepared sample is stable between 4 minutes and 4 hours when stored at 18ºC–30ºC.
Page 743 5. After appropriate incubation, mix the sample again by inverting the sample 5–10 times or vortexing the sample for 5 seconds immediately before aspirating on the ADVIA 2120 / 2120i Hematology System. Cells in prepared CSF samples will settle quickly due to low viscosity.
Page 744 Regulatory ADVIA 2120/2120i Operator’s Guide 2. Using the Order Entry tab, create a workorder for each CSF sample preparation to be analyzed. Include sample physical characteristics in the Ocom field and, if necessary, the dilution factor in the Dil field.
Page 745 ADVIA 2120/2120i Operator’s Guide Regulatory 9. After appropriate incubation, mix the sample again, either by inverting the sample 5–10 times or by vortexing the sample for 5 seconds immediately before aspiration on the ADVIA 120 Hematology System. Cells in prepared CSF samples will settle quickly due to low viscosity.
Page 746 Regulatory ADVIA 2120/2120i Operator’s Guide Quality Control Quality control of the CSF Assay is performed using ADVIA 120 TESTpoint CSF Controls containing one each of Level 1 and Level 2 controls. These controls are intended to be integrated into a clinical laboratory's own quality control program and procedures.
Page 747 (samples prepared 1:1 with CSF reagent) and displays them on the Run Screen. ADVIA 2120/2120i results should be reviewed for conditions listed in the Limitations of the Procedure. If a sample displays any of the listed conditions, the results should be confirmed.
Page 748: Interfering Substances
Regulatory ADVIA 2120/2120i Operator’s Guide 3. The ADVIA 2120/2120i Hematology System provides no morphology flagging to indicate the presence of abnormal cells in the CSF Assay. Only High / Low flagging and the Laser Power Low system flag are enabled for the CSF Assay.
Page 749 ADVIA 2120/2120i Operator’s Guide Regulatory Expected Values The range of expected values for the CSF Assay can vary depending on patient age or other patient characteristics. It is recommended that each laboratory establish its own range of expected values based on their patient population.
Page 750 Regulatory ADVIA 2120/2120i Operator’s Guide Parameter Mean # Lymph 33.8 11.7 # Mono 30.1 # MN 37.9 12.4 # PMN 29.9 Performance Characteristics: Accuracy The accuracy of the CSF WBC count was evaluated by comparing manual counts from 90 samples to counts from the ADVIA 120 Hematology System.
Page 751 ADVIA 2120/2120i Operator’s Guide Regulatory CSF WBC Differential Relative Percentages The accuracy of the CSF WBC Differential relative percentages was evaluated by comparing manual counts from 50 CSF patient samples to counts from the ADVIA 120 Hematology System. The WBC differential relative percentages were analyzed by paired t-test and Rümke analysis as...
Page 752: Intended Use
WBC DIFF Method Intended Use The ADVIA 2120/2120i Hematology System White Blood Cell Differential (WBC DIFF) methods, consisting of both the Peroxidase method and the Basophil/Lobularity method, are intended to quantitatively measure the following WBC hematological parameters: •...
Page 753 ADVIA 2120/2120i Operator’s Guide Regulatory • Monocytes: percentage of WBC (%MONO) and absolute count (#MONO) • Eosinophils: percentage of WBC (%EOS) and absolute count (#EOS) • Large Unstained Cells: percentage of WBC (%LUC) and absolute count (#LUC) • Basophils: percentage of WBC (%BASO) and absolute count (#BASO)
Page 754 Regulatory ADVIA 2120/2120i Operator’s Guide A constant volume of the cell suspension from the Perox reaction chamber passes through the flowcell. The two fluids flow as independent, concentric streams (no mixing), with the ADVIA 120 PEROX SHEATH stream encasing the sample stream. The absorbance and the forward light- scattering signatures of each blood cell are measured.
Page 755 Bibliography Cremins J and Orlik J: Leukocyte differential method. US Patent 5,518,928 (1996) Reagents The following ready-to-use reagents are required to perform the DIFF methods and maintain the ADVIA 2120/2120i Hematology System: DIFF TIMEPAC Symbol Contents Amount (mL) DIFF TIMEPAC...
Page 756 Regulatory ADVIA 2120/2120i Operator’s Guide ADVIA 120 SHEATH/RINSE Symbol Contents Amount (mL) SHEATH/RINSE 10 L SHEATH/RINSE 20 L Amount Contains ADVIA 120 PEROX 1 2 x 650 mL Sodium dodecyl sulfate, 0.36 mmol/L For in vitro diagnostic use. Sorbitol, 620 mmol/L Sodium chloride, 8.35 mmol/L...
Page 757: Storage And Stability
Calibration The system must be calibrated for all parameters except %RETIC. Siemens recommends the use of ADVIA SETpoint Calibrator (Prod. No. T03-3685-01). Please refer to page 4 for product descriptions.
Page 758: Sample Collection
Regulatory ADVIA 2120/2120i Operator’s Guide Calibration procedure should be performed: • Upon installation of the system. • If there is a significant shift in control values after the replacement of a critical hardware component or reagent lot, after checking gains.
Page 759: Materials Required But Not Provided
ADVIA OPTIpoint, and other reagents or equipment specified in the “Methods Introduction“ section. Procedure To run samples on your ADVIA 2120/2120i Hematology system, refer to the Daily Routine. Quality Control It is recommended that the system be controlled using ADVIA TESTpoint Hematology Controls.
Page 760: Limitations Of The Procedure
Regulatory ADVIA 2120/2120i Operator’s Guide Limitations of the Procedure The limitations of the procedure are as listed: 1. Certain abnormal specimens such as those from hemodialysis patients, patient specimens with nucleated RBCs (particularly neonates), as well as specimens exhibiting platelet clumping or incomplete lysis of RBCs, may interfere with white cell differential.
Page 761 ADVIA 2120/2120i Operator’s Guide Regulatory Performance Characteristics: Imprecision A total of 18 runs were performed using multiple samples. The following estimates of imprecision were obtained by taking 25 aspirations from each whole-blood sample. Each of the sample selectivity modes was tested by sampling from opened tubes and with the manual and automated closed-tube samplers.
Page 762 Regulatory ADVIA 2120/2120i Operator’s Guide Reticulocyte Method Intended Use The ADVIA 2120/2120i Hematology System Reticulocyte Count (RETIC) method is intended to quantitatively measure the following reticulocyte parameters: • Reticulocyte concentration (#RETIC) • Reticulocyte percentage (%RETIC) • Reticulocyte Cell Hemoglobin Content (CHr)
Page 763 Fishbane S, et al: Reticulocyte hemoglobin content in the evaluation of iron status in hemodialysis patients. Kidney Intl 52:217-222 (1997) Reagents The following ready-to-use reagents are required to perform the Reticulocyte method and maintain the ADVIA 2120/2120i Hematology System: • ADVIA 120 autoRETIC •...
Page 764 Regulatory ADVIA 2120/2120i Operator’s Guide ADVIA 120 EZ Wash Symbol Contents Amount (mL) EZ Wash 4 x 810 mL EZ Wash 810 mL For a product description of SHEATH/RINSE, see page Chapter 1, Reagents, SHEATH / RINSE, Wash, and DEFOAMER.
Page 765 ADVIA 2120/2120i Operator’s Guide Regulatory Calibrators and Controls ADVIA 120 RETIC Control High Symbol Contents Amount (mL) Reticulocyte Control High 4 x 4.0 mL Reticulocyte Control High 4.0 mL ADVIA 120 RETIC Control Low Symbol Contents Amount (mL) Reticulocyte Control Low 4 x 4.0 mL...
Page 766: Equilibrate Slowly To Room Temperature (15°C To 30°C) Before Mixing
NCCLS or ICSH, or laboratories using certain flow cytometric methods, could observe a significant bias when comparing to the ADVIA 2120/2120i Hematology system method. In this event, use the calibration procedure to obtain agreement with the other method.
Page 767: Stability Of The Least Stable Primary Parameter
ADVIA OPTIpoint, and other reagents or equipment specified in the “Methods Introduction“ section. Procedure To run samples on your ADVIA 2120/2120i Hematology System, refer to the Daily Routine. Quality Control It is recommended that the system be controlled using ADVIA TESTpoint Reticulocyte Controls.
Page 768 Regulatory ADVIA 2120/2120i Operator’s Guide Results The system automatically performs all calculations necessary for obtaining final results. Through the use of complex flagging algorithms, laboratory personnel are alerted to suspected abnormal conditions. These conditions are indicated by the appropriate flag (such as *, +, and/or color highlighting). Whenever flags occur, the user should review the results and take appropriate action.
Page 769 ADVIA 2120/2120i Operator’s Guide Regulatory Performance Characteristics: Imprecision A total of 18 runs were performed using multiple samples. The following estimates of imprecision were obtained by taking 25 aspirations from each whole-blood sample. Each of the sample selectivity modes was tested by sampling from opened tubes, and with the manual and automated closed-tube samplers.
Page 770 ADVIA 2120/2120i Operator’s Guide Performance Characteristics: Carryover Sample carryover for the ADVIA 2120/2120i Hematology System is expressed as the percentage of a high-level sample lost to a trailing low- level sample. Carryover was determined for the RBC count obtained from the reticulocyte channel (RTC RBC).
Page 771: Wbc (10³/Μl)
WBC Data Summary CBC Method Data The sample stability, expected ranges, and performance characteristics for the CBC method are listed in the following Table. Sample Stability Imprecision Correlation Data Analytical Range Room Refrigerated Expected Mean Sy.x Observed Range Deviation Temp Values Carryover 36 h...
Page 772 Sample Stability Imprecision Correlation Data Analytical Range Room Refrigerated Expected Mean Sy.x Observed Range Deviation Temp Values Carryover 48 h 48 h 140 to 360 1.08x - 2.0 0.991 0.1 % 5 to 208 10 x 10³/µL 205 to 4.9 % (10³/µL) 3983 MPV (fL)
Page 773 WBC DIFF Method Data The sample stability, expected ranges, and performance characteristics for the WBC DIFF method are listed in the following Table. Sample Stability Imprecision Correlation Data Room Refrigerated Expected Mean Sy.x Temp Values %NEUT 36 h 72 h 40 to 77 55.0 1.02x - 0.6...
Page 774 Regulatory ADVIA 2120/2120i Operator’s Guide 10708774 Rev.04...
Page 775 Overview The optional ADVIA® Autoslide Slide Maker Stainer (SMS) is connected to the ADVIA 2120/2120i Hematology System and controlled by the ADVIA 2120/2120i system software. The SMS can automatically prepare and stain a high-quality blood smear on a microscope slide for white blood cell (WBC) differential analysis and microscope examination.
Page 776 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide Slide Dispenser WARNING Do not try to empty the slide dispenser! Finger injuries can occur if slides are removed from the slide-dispenser assembly. Avoid any unnecessary contact with the slide-dispenser mechanical device. The slide-dispenser cover cannot be removed without using a tool. Do not disable the sensors.
Page 777 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer Stain Overflow Collection Tray WARNING Staining reagents contain methanol that is flammable and toxic. To avoid personal injury and damage to the system, inspect the reagent overflow tray periodically to verify that there are no leaks of the reagents.
Page 778 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide Autoslide Rear Panel Rinse Buffer Water Water Sensor Stain Waste Sensor Methanol Stain 1 Stain 2 Stain Waste CAUTION Do not obstruct the fan. Obstructing the fan can cause damage to the instrument.
Page 779 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer Use only main supply cable delivered with the instrument or cable with same characteristics: • Europe cord type: HO5VV-F • USA cord type: SVT,AWG 18-3 Main supply voltage fluctuations not to exceed ± 10% of the nominal voltage.
Page 780 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide Autoslide Safety Information and Warnings NOTE: Installation, connection, and disconnection of the ADVIA Autoslide Slide Maker Stainer must be performed by authorized personnel only. Before moving the instrument, contact your local technical support provider for assistance.
Page 781 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer METHANOL SAFETY Toxic! Toxic: danger of very serious irreversible effects through inhalation, in contact with skin, and if swallowed. Keep container tightly closed. Avoid contact with skin. Wear suitable protective clothing and gloves. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Page 782: Theory Of Operation
Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide Theory of Operation Slide dispenser Reagent syringes Drop needle Rack out queue Slide carrier 10 Verticalizer Sampling syringes 11 Rack transfer Smearer 12 Slide transfer/grabber Printer 13 Manual inlet Stainer assembly 14 Rack loader...
Page 783 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer Slide Process Drop needle Verticalizer Smearing assembly Stainer Slide printer Rack 1. The drop needle dispenses a drop of the sample on a slide. The drop needed dispenses the rest of the sample into the drop needle rinse bath and then is rinsed with rinse.
Page 784 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide 5. If staining is required, the slide moves into the stainer, a rotating tray containing 32 staining wells. A needle plate supports needles at adjustable locations to dispense liquids into the wells or to aspirate liquid from the wells.
Page 785: Startup Tab
ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer Setup and Use Operations Menu Startup Tab This window displays the status of the reagents and supplies necessary for operating the Autoslide. The background color of each field changes depending on the alarm criteria that have been defined.
Page 786 NOTE: Use the Autoslide to stain slides that have been manually smeared. a. Ensure the ADVIA 2120/2120i system is not processing samples. b. At the Operations menu, select Autoslide Control. c. In the Autoslide Control tab, select the Manual/Inlet Door option, then select Start.
Page 787 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer 6. Replace, tighten, or adjust the inventory of the smearing tape: a. To replace or tighten the smearing tape: • Select the Smearing Tape and Replace Tape options. • Follow the instructions that display in the window.
Page 788 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide Logs Menu Autoslide Reagent Log Select Reagent Inventory to view the window and enter the lot number and expiration date for each new installed reagent container. Select Reagent Installation to view the window that identifies every reagent container used in the system.
Page 789 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer 2. At the Logs menu, select Reagent Log and then select Autoslide Reagent Log. The software opens to the Reagent Inventory window. 3. Select Reagent Installation. 4. For each new reagent, enter the Reagent Volume, Lot #, and Expiration Date.
Page 790 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide Cumulative Inventory Window Determine the number of reagent containers used over a specific period of time: 1. In the Start Date box, enter the beginning date directly or select the down arrow, then select the date from the calendar.
Page 791 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer Search for a slide: 1. Select Slide Search to open the Searching for Slides dialog box. 2. Select the searching method to use and enter the necessary search parameters. 3. Select OK. Viewing Slide Details Each entry in the Slide Log contains additional information that does not display in the main list.
Page 792 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide Deleting Slide Log Entries You can delete all the entries in the slide log or selected entries. 1. From the pull-down menu next to the Delete button, select the deletion method to use: All or Selected.
Page 793 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer Utilities Menu Hydraulic Function Tab Use the Hydraulic Functions tab to start one of the Autoslide hydraulic operations: WARNING Staining reagents contain methanol that is flammable and toxic. To avoid personal injury and damage to the instrument, you must check the reagent overflow tray periodically to verify there are no reagent leaks.
Page 794 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide Reagent Prime Primes the selected reagent line Methanol Fill Fills all staining wells with methanol Methanol Drain Drains methanol from all staining wells Performing a hydraulic function 1. Select the function you want to perform.
Page 795 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer Staining Wells Opens a window that indicates the status of each staining well. Error Info Opens a window that displays a list of Autoslide errors and the context in which they occurred. Autoslide Status Opens a window that displays a list of Autoslide errors and the context in which they occurred.
Page 796 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide Auto System Wash The system wash cleans the analyzer’s hydraulic pathways, chambers, and flowcells with EZ Wash followed by a SHEATH/RINSE. Set the analyzer to automatically perform up to three full system wash cycles up to four time a day.
Page 797: Auto Standby
ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer Auto Rinse The AutoRinse feature sets the analyzer to automatically rinse the hydraulic pathways, chambers, and flowcells whenever it is idle for a specified period of time after processing a sample. Scheduling an automatic rinse cycle: 1.
Page 798: Auto Startup
Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide Auto Startup The Auto Startup feature sets the analyzer for automatically performing the system startup process at a specific time every day. The Auto Startup feature also sets the system to automatically print a copy of the Startup tab upon completion of the startup process.
Page 799: Setup Tab
ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer Setup Tab Slide Options The Slide Options window allows for setting the criteria that determines whether the system automatically prepares a slide for a sample. Enable Slide Requests Selecting this check box enables preparation of the slides.
Page 800 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide • Enable Criteria on Manual Samples Select this option causes the system to add a "Slide" test to the workorder of any manually aspirated sample that meets the slide by exception criteria. This criteria displays necessary slide production.
Page 801 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer Ranges This window allows selecting all or individual test result ranges for triggering slide production by exception. 1. Select the check box for each range using as a trigger for slide making. Clear a selection by selecting it again.
Page 802 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide 4. Select Cancel to undo changes and return the settings to the last saved configuration. 5. After selecting the appropriate settings, select Save before exiting the window. Location This window allows triggering of slide production for specific locations.
Page 803 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer 3. Select Cancel to undo changes and return the settings to the last saved configuration. 4. Clear an age range by entering 0 in both the From and To fields. 5. After selecting the appropriate settings, select Save before exiting the window.
Page 804 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide Smear Characteristics Characteristic Thickness Length Spreader Angle 40° Thin Long Spreader Angle 45° Thick Short Smearing Speed Slow Thin Long Smearing Speed Fast Thick Short Drop Volume Small Thin Short Drop Volume Large...
Page 805: Staining Protocol
NOTE: Change staining protocol to be used only when the ADVIA 2120/2120i system is in a configuration or diagnostics mode and not aspirating samples and the Autoslide is idle. Make appropriate hardware changes (Example: needle positions) when selecting a different staining protocol for use.
Page 806 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide The five dispensing needles include: • DN1 dispenses undiluted stain #1 • DN2 dispenses buffer • DN3 dispenses diluted stain #2 • DN4 dispenses Methanol • DN7 dispenses buffer or water (if configured) The four aspirating needles include: •...
Page 807 3. After selecting the settings, select Save before exiting the window. Autoslide Alarm / Stop CAUTION To avoid damage to the system, Siemens Healthineers strongly recommends that you do not change the Autoslide Alarm/Stop settings from the default values. This window specifies the alarm stop criteria for the following conditions: •...
Page 808: Loading Slides
Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide Loading Slide Racks • Load at least 2 racks into the rack loader. The rack loader can hold up to 9 racks at a time. • Load the racks as shown below, with the slide grooves facing upwards.
Page 809 Slide Maker Stainer Slides should be allowed to come to Lab temperature, before they are opened. • Use slides provided by Siemens approved vendors only. Size: 76 x 26 x 1mm Description: ground edges, rounded corners • Avoid touching slide surfaces with your fingers.
Page 810 Staining Manually Smeared Slides You can use the Autoslide module to stain slides that have been manually smeared away from the system. 1. Ensure the ADVIA 2120/2120i system is not processing samples and is idle. 2. At the Operations menu, select Autoslide Control.
Page 811 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer 3. In the Autoslide Control tab, select the Manual/STAT Door option, then select Start. 4. When the door opens, insert the slide into the holder door with blood smear on the right with the frosted end up.
Page 812 NOTE: Ensure that no slides are present in the Autoslide Stainer. 2. Place the ADVIA 2120/2120i system in Standby. 3. Select Off on the control panel to shut off the ADVIA 2120/2120i system. This prevents any further system function. The system displays the following message, communication error with Autoslide.
Page 813 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer 4. Unscrew the Autoslide sample blood line. Blood line 5. Pry open the protective conduit tubing where the vacuum and pressure lines reside. Vacuum line Pressure line 10708774 Rev.04...
Page 814 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide 6. Push the metal lever and pull the vacuum line to disconnect. Vacuum line 7. Push the metal lever and pull the pressure line to disconnect. Pressure line 8. Loosen the 2 screws where the slots are on the Autoslide cart bracket so you can move the support structure.
Page 815 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer 9. Move the support structure forward up to 0.3 m (1 foot), ensuring that the electrical cables are not stretched to far. Blood sample line Pressure line Ground strap Vacuum line NOTE: The ground strap and interface cable remain connected to the Autoslide.
Page 816: Intended Use
The ADVIA Autoslide Slide Maker Stainer is a smart, fully automated system. The stainer is capable of producing stained smears on all specimens run on the ADVIA 2120/2120i or only on samples with predefined criteria (i.e. flags or parameter threshold). The Autoslide module also provides a direct access port to the stainer for pre-smeared slides.
Page 817 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer 10. Dispensing rinsing solution (distilled water) (DN7). 11. Draining (AN7). 12. Draining (AN4). 13. Dispensing methanol (DN4). 14. Slide removal. Needle Position Action DN1 (Undil. Stain 1) DN2 (Buffer) DN3 (Dil. Stain 2)
Page 818 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide Cell Type Cell Component Color Cytoplasm Blue-Gray Granules Fine Pink to Violet Eosinophils Nuclei Pale Purplish-Blue Cytoplasm Bluish Pink Granules Orange Basophils Nuclei Purplish-Blue Cytoplasm Faint Pink Granules Deep Purple Platelets Cytoplasm Light Blue...
Page 819 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer Reagents and Supplies For in vitro diagnostic use. Reagents are ready to use and require no preparation. Contents Symbols May-Grünwald Stain 1 Giemsa Stain 2 May-Grünwald Giemsa Buffer Methanol ADVIA Autoslide Rinse Stain 1 May-Grünwald Stain •...
Page 820 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide Stain 2 - Giemsa Stain • Methanol, 56 % • Glycerol, 43 % • Methylene blue - Eosin, 1 % Toxic! Toxic: danger of very serious irreversible effects through inhalation, in contact with skin, and if swallowed. Keep container tightly closed.
Page 821 15°C and 30°C. Once opened, reagents are stable for 45 days. Do not refrigerate. Refer to the online ADVIA 2120/2120i Operator's Guide for information on specimen collection. Material required but not provided Microscope slides (26 x 76 x 1 mm)
Page 822 The ADVIA Autoslide Slide Maker Stainer is a smart, fully automated system that is capable of producing stained smears on all specimens run on the ADVIA 2120 / 2120i or only on samples with predefined criteria (i.e. flags or parameter threshold). The Autoslide provides also a direct access port to the stainer for pre-smeared slides.
Page 823 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer 6. Dispensing rinsing solution (distilled water) (DN7). 7. Draining (AN7). 8. Dispensing rinsing solution (distilled water) (DN7). 9. Draining (AN4). 10. Dispensing methanol (AN7). 11. Slide removal. Needle Position Action DN1 (Undil. Stain 1) DN2 (Buffer) DN3 (Dil.
Page 824 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide Cell Type Cell Component Color Monocytes Nuclei Light purple with distinct spaces between chromatin strands Cytoplasm Dull blue-gray with lightly stained Granules Fine pink to violet and evenly distributed Eosinophils Nuclei Pale purplish-blue and well...
Page 825 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer Reagents and Supplies For in vitro diagnostic use. Reagents are ready to use and require no preparation. Contents Symbol Modified Wright Stain Modified Wright Buffer Methanol ADVIA Autoslide Rinse Modified Wright Stain •...
Page 826 15°C and 30°C. Once opened, reagents are stable for 45 days. Do not refrigerate. Refer to the online ADVIA 2120/2120i Operator's Guide for information on specimen collection. Material required but not provided Microscope slides (26 x 76 x 1 mm)
Page 827 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer Limitations Modified Wright reagents produce high quality stained blood films. However, there may be personal preferences in shades and intensities of staining not satisfied by the system. Results When used with the ADVIA Autoslide Slide Maker Stainer, the Modified Wright reagents will produce a consistent stain quality to facilitate accurate and reliable interpretation.
Page 828 The ADVIA Autoslide Slide Maker Stainer is a smart, fully automated system that is capable of producing stained smears on all specimens run on the ADVIA 2120/2120i or only on samples with predefined criteria (i.e. flags or parameter threshold). The Autoslide also provides a direct access port to the stainer for pre-smeared slides.
Page 829 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer NOTE: Needles AN2 and DN2 in position 22 are unused in this protocol. Methanol Volume (mL) Stain Volume (mL) Dilution Ratio (%) Duration Long 10708774 Rev.04...
Page 830 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide Principle of the Procedure The stained smear is examined microscopically; the nucleus and the cytoplasm of neutrophils, lymphocytes, monocytes, eosinophils, and basophils show a characteristic blue or red coloration. Cells are then manually differentiated and quantified.
Page 831 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer Cell Component Color Cell Type Granules Dark blue to reddish blue Promyelocyte Cytoplasm Purplish-red Auer Rods Causes of RBC Artifacts • Post-stain rinsing too short • Reagents expired • Stain open for extended period •...
Page 832 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide Wright Giemsa Stain • Methanol, > 99 % • Polychrome methylene blue-eosin stain Toxic! Toxic: danger of very serious irreversible effects through inhalation, in contact with skin, and if swallowed. Keep container tightly closed. Avoid contact with skin. Wear suitable protective clothing and gloves.
Page 833 15°C and 30°C. Once opened, reagents are stable for 45 days. Do not refrigerate. Refer to the online ADVIA 2120/2120i Operator's Guide for information on specimen collection. Material required but not provided Microscope slides (26 x 76 x 1 mm)
Page 834: Periodic Maintenance
Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide 2. White WL, Erickson MM, Stevens SC. Practical Automation for the Clinical Laboratory. St. Louis, MO, CV Mosby Co., pp 476-487 (1972) 3. Moss ED. Automated Slide Staining in Haematology. Can. J. Med. Tech.
Page 835 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer Removing glass particle waste Small particles of glass may be chipped off the slides during transfer from the slide dispenser to the slide carrier. These particles are collected in a tray beneath the slide dispenser.
Page 836 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide Cleaning the Stain Overflow TraY BIOHAZARD All products or objects that come in contact with human or animal body fluids should be handled, before and after cleaning, as if capable of transmitting infectious diseases. Wear facial protection, gloves, and protective clothing.
Page 837 FLAMMABLE Methanol is flammable. Keep away from sources of ignition - no smoking. 1. On the ADVIA 2120/2120i software menu, select Procedures, then select Clean Autoslide. The Clean Autoslide window opens. 2. Select Weekly Shutdown and follow the instructions displayed on the window to perform a weekly shutdown.
Page 838 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide Materials required: • Methanol • Laboratory tray • Paper towels Time: 15 minutes Analyzer mode: Standby NOTE: It is recommended that the stainer wells be replaced once a year. Check the Appendix C, Orderable Parts section of the Operator's Guide for ordering information.
Page 839 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer 3. While manually rotating the stainer plate, remove the stainer wells by gently sliding each out of the stainer. CAUTION Do not put too much force on the staining wells, when removing them.
Page 840 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide NOTE: If the wells are very loose they will slide on or off the stainer plate with little or no resistance, replace the well. 5. After the wells have soaked in the methanol for ten minutes, remove them from the tray and dry them with paper towels.
Page 841: Cleaning The Fan Filter
ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer Cleaning the Fan Filter 1. At the back of the Autoslide, turn off the power. 2. Remove the fan cover and separate the filter. 3. Using a vacuum or air canister, clean all dust and dirt from the filter.
Page 842 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide 5. Using the black magnetic knob, open the tape holder plate. 6. Remove the upper and lower bobbins from their holders. 7. Each time the smearing tape is replaced, wipe the wedge use an alcohol swab and soft cloth to remove any debris.
Page 843 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer 9. Close the tape holder plate. 10. At the Autoslide Control window, select Start to tighten the tape. Replacing the Printer Ribbon Materials required: New print cartridge Time: 2 minutes Analyzer mode: Standby...
Page 844 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide 3. Holding both sides of the printer cartridge, press toward the inside of the Autoslide and pull the cartridge out of its holder. Be careful not to tangle the ribbon when removing the ribbon.
Page 845 30 seconds for a response from the system. This is due to communication restraints between the ADVIA 2120/2120i system and the Autoslide. It is important to allow time for the system to respond - DO NOT keep clicking the mouse.
Page 846 Details for additional information about the problem. TIP # 5 If, for any reason, the power needs to be cycled to the Autoslide, allow 30 seconds between cycling for the instrument reconnect the communication link with the ADVIA 2120/2120i system, do not interrupt. 10708774 Rev.04...
Page 847 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer The table below Event/cause/suggested actions supercedes the table currently displayed in the Operator's Guide. Event Text Severity Probable Cause Suggested Action ASL - Aspiration Failure Aspiration needle Reset the needle retract failed to retract in...
Page 848 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide Event Text Severity Probable Cause Suggested Action Perform Mechanical ASL - Failure An invalid parameter Initialization to reset Configuration / was used to Autoslide, and report Parameter error configure the the problem to your Autoslide.
Page 849 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer Event Text Severity Probable Cause Suggested Action ASL - EEPROM IO Failure An error occurred when Perform Mechanical reading or updating the Initialization to reset EEPROM. Autoslide, Cycle power to Autoslide if problem...
Page 850 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide Event Text Severity Probable Cause Suggested Action ASL - Internal Failure Autoslide internal Perform a mechanical synchronization synchronization error. initialization. If error problem persists, contact your local technical support provider for assistance. If you have any...
Page 851 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer Event Text Severity Probable Cause Suggested Action ASL - Rack Error - Failure General rack problem, Replenish racks in Stop could be low on racks. dispenser, check for jammed rack or full ejection tray. Run a reset smearer.
Page 852 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide Event Text Severity Probable Cause Suggested Action Failure Perform the smearing ASL - Smearing Smearing film empty, film replacement tape is loose broken or incorrectly procedure. Be sure to threaded. check smearing wedge for cleanliness.
Page 853 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer Event Text Severity Probable Cause Suggested Action Failure Autoslide reported Contact your local ASL - Unknown technical support an error error provider for assistance. unrecognized by the If you have any ADVIA 2120/2120i questions or concerns system.
Page 854 Slide Maker Stainer ADVIA 2120/2120i Operator’s Guide Event Text Severity Probable Cause Suggested Action Autoslide Daily Warning An error occurred that Check message log for Shutdown prevented the errors, correct any Incomplete Autoslide daily problems, and retry the shutdown from daily shutdown.
Page 855 ADVIA 2120/2120i Operator’s Guide Slide Maker Stainer Event Text Severity Probable Cause Suggested Action Warning Check message log for Autoslide Weekly An error occurred Shutdown during the weekly other errors, correct Incomplete shutdown problem, reset Autoslide, and repeat weekly shutdown.
Page 856 From 100V to 240V ± 10% Maximum: 200 VA Slide Requirements rounded corner, ground edges 76 mm x 26 mm x 1 mm Use slides provided by Siemens approved vendors only. Sample Requirement NOTE: 75 µL whole blood 10708774 Rev.04...
Page 857: Appendix A: Legal Information
Limited Instrument Warranty and Service Delivery Policy Siemens and its authorized distributors may provide customers who acquire new Siemens instruments with a limited warranty either in a specific agreement or in standard language on their invoices. This limited warranty is designed to protect customers from the cost associated with repairing instruments that exhibit malfunctions due to defects in materials and/or workmanship during the warranty period.
Page 858 1 year thereafter, unless otherwise specifically agreed to by and between Siemens (or its authorized distributors) and customer in a writing signed by duly authorized representatives of both parties (sales representatives are generally not authorized representatives of Siemens for these purposes).
Page 859 Maintenance Items for any specific model of instrument. Design Changes and Retrofitting of Instruments Siemens reserves the right to change the design or construction of specific models of instruments at any time without incurring any obligation to make such changes available to individual customers or instruments.
Page 860: Warranty And Service Exclusions
4. Customer did not purchase the instrument from Siemens or one of its authorized distributors. 5. The instrument has not been installed within 90 days of shipment to the customer's facility unless otherwise specified.
Page 861 EQUIPMENT AND SHALL NOT INCLUDE ANY DAMAGES OF ANY KIND, WHETHER DIRECT, INDIRECT, INCIDENTAL, CONTINGENT, OR CONSEQUENTIAL. SIEMENS SHALL NOT BE LIABLE FOR DELAY FROM ANY CAUSE IN PROVIDING REPAIR OR EXCHANGE SERVICE. ANY LIMITATIONS OR OTHER PROVISIONS NOT CONSISTENT WITH...
Page 862 Legal Information ADVIA 2120/2120i Operator’s Guide 10708774 Rev.04...
Page 863: Appendix B: Safety
To avoid damage to the eyes, never look directly at the laser beam or at its reflection from a shiny surface. All field service procedures must be followed precisely. Only Siemens-trained field service personnel should perform procedures related to laser assemblies.
Page 864: Safety Information
Regular strength household bleach is 5% sodium hypochlorite. Extra- strength household bleach is 6% sodium hypochlorite. Either strength may be used with the ADVIA 2120/2120i Hematology System. When handling bleach, which can be used as a cleaning and antiviral agent, wear protective clothing, gloves, and safety glasses.
Page 865 CE mark requirements The ADVIA 2120/2120i conforms to the following standard, and therefore meets the EMC conformity requirements for CE marking according to the European IVDD Directive 98/79 EC. EN27779: 1989...
Page 866: System Symbols
System Symbols This section describes the symbols that can appear on the exterior of the ADVIA 2120/2120i system or on the system packaging. The symbols on the system provide you with the location of certain components and with warnings for proper operation. The symbols on the system packaging provide you with other important information.
Page 867 ADVIA 2120/2120i Operator’s Guide Safety Symbol Description Biohazard. The biohazard warning label mounted on the front of the analyzer alerts you to the possibility of exposure to a biohazard during the sampling process or through contamination of the analyzer. This symbol identifies a product that may contain an infectious agent.
Page 868 Safety ADVIA 2120/2120i Operator’s Guide Symbol Description This symbol indicates the manufacturer's authorized representative within the European community. This symbol indicates that the product complies with the applicable directives of the European Union. This symbol indicates that the product was IEC 61010-1 safety tested by TUV for conformity to global markets including Canada, US, and EU.
Page 869 ADVIA 2120/2120i Operator’s Guide Safety Symbol Description This symbol indicates the switch position for emptying the waste container. This symbol indicates the need to empty the waste container. This symbol indicates the open and closed positions for a spigot. This symbol indicates liquid waste.
Page 870 Safety ADVIA 2120/2120i Operator’s Guide Symbol Description This is the eject rack symbol. This is the network symbol. This is the monitor symbol. This symbol indicates the maximum level. This is the knob symbol. This is the fuse symbol. This is the fan symbol.
Page 871 ADVIA 2120/2120i Operator’s Guide Safety Symbol Description This symbol indicates that moving the component can cause injury. This symbol identifies a product that contains recyclable material. This symbol indicates the top of the package. This symbol indicates the acceptable temperature range for storage of the product.
Page 872 Safety ADVIA 2120/2120i Operator’s Guide Symbol Description This is the CSF Control 1 symbol. This is the CSF Control 2 symbol. This is the ADVIA RETIC High Control symbol. This is the ADVIA RETIC Low Control symbol. This is the DEFOAMER symbol.
Page 873: Interpretation Of Results
Siemens products do not make diagnoses on patients. Siemens intends its diagnostic products (systems, reagents, software, hardware) to be used to collect data reflecting the patient's chemical, hematological, or immunological status at a certain point in time.
Page 874 Safety ADVIA 2120/2120i Operator’s Guide 5. Always replace a fuse with one of the same type and rating. If the fuse label is missing or illegible, refer to the fuse information table. Be sure to select the fuse requirements appropriate for your system's input voltage.
Page 875 ADVIA 2120/2120i Operator’s Guide Safety Protecting yourself from lasers The ADVIA 2120/2120i Hematology System is classified as a Class I laser product as defined by the National Center for Devices and Radiological Health (CDRH) regulations 21 CFR 1040 and by EN-60825.
Page 876 All field service procedures must be followed precisely to prevent possible eye injury from the laser radiation. Only Siemens-trained field service personnel should perform procedures related to the ADVIA 2120/2120i laser optics bench.
Page 877: Appendix C: Orderable Parts
Appendix C: Orderable Parts Consumable Parts Part Description Siemens Material Number (SMN) 1000µL Syringe NEW 3M 11220533 50µL Syringe NEW 3M 11220532 Clot Filter 10310090 Defoamer Straw 10309253 Drain Filter 10311260 Filter 0.22mm RBC Sheath 10311257 Filter 0.45mm PEROX Sheath...
Page 878: Customer Replaceable Parts
Orderable Parts ADVIA 2120/2120i Operator’s Guide Customer Replaceable Parts Part Description Siemens Material Number (SMN) Autoslide Consumables 10325262 Autoslide Rinse 1 x 10L Matsunami slides (3000 slides) 10699575 Siemens (1440 slides) 10317614 Thermo Scientific (5000 slides) 10714500 Methanol May Gruenwald Stain Wright Stain 4 x 3.8L 10327567...
Page 879 ADVIA 2120/2120i Operator’s Guide Orderable Parts Part Description Siemens Material Number (SMN) Gray Flared Tube Assy to Perox Dil 3 .040 ID 11222926 Green Flared Tube Assy to Perox Dil 1 .040 ID 11222924 Flared Sample Tubing Clot Filter to UFC .025 ID...
Page 880 Orderable Parts ADVIA 2120/2120i Operator’s Guide 10708774 Rev.04...
Page 881: Appendix D: Specifications
Appendix D: Specifications Autosampler Sample Capacity 150 samples 15 racks of 10 tubes Tube Sizes 10–13 mm diameter 50–100 mm height Tube Types Some of the allowable tube types: • Standard VACUTAINER® • HEMOGARD • Center puncture Monovette • Venoject® II Barcode reader Reads up to 14 digits Automatic label code discrimination...
Page 882 Specifications ADVIA 2120/2120i Operator’s Guide • Quality control 3D bar graph Levey-Jennings plot SDI graph Table format • Remote QC • ILQC programs • Patient moving average • User assistance Context sensitive help Operator's guide Procedure wizards Problem solving diagnostics...
Page 883 ADVIA 2120/2120i Operator’s Guide Specifications Morphology Results (user definable): WBC: Left Shift, Atypical Lymph, Blasts , Immature Granulocytes, Myeloperoxidase Deficiency RBC: ANISO, MICRO, MACRO, HC VAR, HYPO, HYPER, RBC Fragments, RBC Ghosts, NRBC, Platelet Clumps, Large Platelets Performance Specifications Analytical Ranges (Linearity) 0.02–400 x 10...
Page 884 Azide-free reagents drain into waste container with automatic level-sensor shutoff. Waste per CBC/diff/retic cycle, including rinse: 23 mL The ADVIA 2120/2120i Hematology System is for indoor use only. Operation of the instrument at altitudes of over 2000 meters (6000 feet) is not recommended.
Page 885 ADVIA 2120/2120i Operator’s Guide Specifications Personal Computer Monitor Weight 10.6 cm (4.2 in) 7.0 kg (15.43 lbs) Height 38.9 cm (15.3 in) Extended in Landscape: 54.6 cm (21.50 in) Compressed in Portrait: 38.0 cm (14.96 in) Width 38.9 cm (15.3 in) 37.6 cm (14.8 in)
Page 886 Specifications ADVIA 2120/2120i Operator’s Guide 10708774 Rev.04...
Page 887: Appendix E Revision History
Appendix E Revision History New Topics • Working with the Software • Orderable Parts appendix • Serious Incident Reporting Updated Topics • Added IVDR information to the Overview chapter • Added Quality Control statement to the Overview in the Regulatory chapter •...
Page 888 Revision History ADVIA 2120/2120i Operator’s Guide 10708774 Rev.04...

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Advia 2120i