Neogen Reveal Q+ Manual

For t-2/ht-2, for use with neogen's line of accuscan readers including accuscan pro and accuscan gold

Advertisement

Available languages

Available languages

Product 8285
Read instructions carefully before starting test
for T-2/HT-2
For use with Neogen's line of AccuScan
readers,
®
including AccuScan Pro and AccuScan Gold
THE TOXIN
T-2/HT-2 toxins are trichothecene mycotoxins produced by several species of
Fusarium molds. Since T-2 toxin is readily metabolized to HT-2 toxin and the toxins
have been shown to produce numerous adverse effects on many animals, these
two mycotoxins are frequently evaluated together.
Animals affected by the toxins include swine, dairy cattle, poultry, dogs, cats
and horses. Effects of the toxins include digestive disorders, hemorrhage,
edema, oral lesions, dermatitis, and blood disorders. Damage caused by the
toxins to the digestive track is ir reversible. In the most severe cases, these
toxins will cause death. T-2 toxin is the principal causal toxin in the human
disease alimentary toxic aleukia.
Poultry studies have shown T-2 intoxication has led to a reduction in weight
gain and other problems such as beak lesions, poor feathering, motor function
impairment and increased susceptibility to Salmonella spp.
The best protection against these mycotoxins is monitoring for their presence in
feeds and foods. That means testing all along the pathway from initial harvest of
grains to the finished product.

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the Reveal Q+ and is the answer not in the manual?

Questions and answers

Summary of Contents for Neogen Reveal Q+

  • Page 1 Product 8285 Read instructions carefully before starting test for T-2/HT-2 For use with Neogen’s line of AccuScan readers, ® including AccuScan Pro and AccuScan Gold THE TOXIN T-2/HT-2 toxins are trichothecene mycotoxins produced by several species of Fusarium molds. Since T-2 toxin is readily metabolized to HT-2 toxin and the toxins have been shown to produce numerous adverse effects on many animals, these two mycotoxins are frequently evaluated together.
  • Page 2 1. Sample collection cups with lids (NEOGEN item 9428) 2. Agri-Grind grinder or equivalent (NEOGEN item 9401, 9453) 3. Scale capable of weighing 5–50 g ± 0.1 g (NEOGEN item 9427) 4. Timer (NEOGEN item 9426) 5. Reveal sample cup rack (NEOGEN item 9475) 6.
  • Page 3 8. Filter syringe (NEOGEN item 9420) Centrifuge, mini (NEOGEN item 9330) Microcentrifuge tubes (NEOGEN item 9172) 9. Sample collection tubes with caps (NEOGEN item 9421, 9421B) 10. Pipettor, 100 µL (NEOGEN item 9272, 9278) 11. Pipette tips, 100 µL (Neogen item 9407, 9410, 9417) 12.
  • Page 4: Sample Preparation

    SAMPLE PREPARATION The sample to be tested should be collected according to accepted sampling techniques (see FGIS sampling protocol or contact your NEOGEN representative). Obtain a representative sample (minimum 100 g). Grind the sample so at least 95% of the ground material passes through a 20- mesh sieve (about the particle size of fine espresso).
  • Page 5 2. Readings should be made within 30 seconds of the 6 minute incubation time. Readings after 6.5 minutes may be inaccurate due to over-development of the device. 3. The strips must be read using NEOGEN’s AccuScan Pro or AccuScan Gold readers. Reveal Q+ for T-2/HT-2...
  • Page 6: Warranty

    Training on this product, and all NEOGEN test kits, is available. SDS INFORMATION AVAILABLE Safety data sheets (SDS) are available for this test kit on NEOGEN’s website at NEOGEN.com, or by calling NEOGEN at 800.234.5333 or 517.372.9200. TERMS AND CONDITIONS For NEOGEN’s full terms and conditions, please visit www.NEOGEN.com/en/...
  • Page 7 NOTES: ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ ________________________________________________________________ Reveal Q+ for T-2/HT-2...
  • Page 8 +52 (55) 5254-8235 +91 484 2306598, 2301582 informacion@neogenlac.com info@neogenindia.com NEOGEN.com www.neogenindia.com ©NEOGEN Corporation, 2020. NEOGEN, AccuScan and Reveal are registered trademarks of NEOGEN Corporation. All other brand and product names are trademarks or registered trademarks of their respective companies. https://www.neogen.com/en/patents 16288F 8285RevealQ+T2HT2...
  • Page 9 Producto 8285 Lea las instrucciones con cuidado antes de comenzar la prueba para T-2/HT-2 Para uso con la línea de lectores AccuScan® de NEOGEN, incluyendo AccuScan Pro y AccuScan Gold LA TOXINA Las toxinas T-2/HT-2 son micotoxinas de la familia de los tricotecenos, producidas por varias especies de mohos Fusarium.
  • Page 10: Materiales Incluidos

    USO/USUARIO PREVISTO Reveal® Q+ para T-2/HT-2 está destinado para el análisis cuantitativo de las toxinas T-2/HT-2 en maíz y productos de maíz. El kit de prueba está diseñado para ser utilizado por personal de control de calidad y otras personas familiarizadas con productos posiblemente contaminados con las toxinas T-2/HT-2.
  • Page 11 Centrífuga, mini (artículo de NEOGEN 9330) Tubos para microcentrífuga (artículo de NEOGEN 9172) 9. Tubos de recolección de muestra con tapa (artículos de NEOGEN 9421 y 9421B) 10. Pipeta, 100 µL (artículos de NEOGEN 9272 y 9278) 11. Puntas para pipeta, 100 μL (artículos de NEOGEN 9407, 9410 y 9417) 12.
  • Page 12 La muestra por analizar se debe recolectar conforme a las técnicas de muestreo aceptadas (consulte el protocolo de muestreo del FGIS o contacte a su representante de NEOGEN). Obtenga una muestra representativa (mínimo 100 g). Muela la muestra de forma que al menos 95 % del material molido pase a través de un tamiz de malla 20 (alrededor del tamaño de partícula de...
  • Page 13 Procedimiento de prueba 1. Coloque el número apropiado de recipientes rojos de dilución de muestra y recipientes de muestra transparentes en una gradilla de recipientes de muestra. Etiquete los recipientes conforme sea necesario 2. Agregue 100 μL de extracto de muestra al recipiente rojo para muestra. 3.
  • Page 14 NOTA: Las muestras mayores a 600 ppb se deben diluir y volver a evaluar. Los resultados inferiores al margen de cuantificación se deben informar como menores a 50 ppb. Matrices validadas/verificadas NEOGEN continúa validando nuevos productos. Contacte a un representante para consultar la lista más reciente de productos validados. Reveal Q+ para T-2/HT-2...
  • Page 15: Servicio Al Cliente

    NEOGEN. INFORMACIÓN DE SDS DISPONIBLE Hay fichas de datos de seguridad (SDS) disponibles para este kit de prueba en el sitio de internet de NEOGEN en NEOGEN.com o llamando a NEOGEN al 800.234.5333 o al 517.372.9200. TÉRMINOS Y CONDICIONES Para ver los términos y las condiciones completas de NEOGEN, visite www.
  • Page 16 NEOGEN.com ©NEOGEN Corporation, 2020. NEOGEN, AccuScan y Reveal son marcas comerciales registradas de NEOGEN Corporation. Todas las demás marcas y nombres de producto son marcas comerciales o marcas comerciales registradas de sus respectivas compañías. https://www.NEOGEN.com/en/patents 16288F 8285RevealQ+T2HT2...

This manual is also suitable for:

8285

Table of Contents