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Summary of Contents for Sartorius rLine 5-200ul LS
- Page 1 Sartorius rLine Technical User Manual Includes rLine models: rLine 1-ch 5-200ul LS rLine 1-ch 50-1000ul LS rLine 1-ch 100-5000ul APPROVED April 18...
- Page 2 Version history Version Date / Author Reason 14.12.2017 / Mhe Approved first version 04.04.2018 / Mhe Added RBxxx command for new firmwares APPROVED April 18...
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Page 3: Table Of Contents
1 Contents INTRODUCTION ........................... 5 BASICS OF LIQUID HANDLING ...................... 6 RLINE TERMINOLOGY ........................8 Step ............................8 Address ........................... 8 Position ..........................8 Drive cycle ..........................9 Level sensing, LS ........................9 TECHNICAL SPECIFICATIONS ...................... 10 Liquid handling specifications ....................10 Aspirating and dispensing speeds .................. - Page 4 PC SOFTWARE ........................... 26 ORDER INFORMATION ......................27 APPROVED April 18...
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Page 5: Introduction
2 Introduction Sartorius rLine is a unique liquid handling dispenser module designed for various liquid handling automation applications. All rLine models are extremely small and lightweight. They use disposable tips for zero carry-over and thanks to novel electronic tip ejector the tip can be disposed anywhere – or even returned back for reuse. -
Page 6: Basics Of Liquid Handling
3 Basics of liquid handling The liquid handling of the rLine module is based on the air displacement technique where a piston movement creates a vacuum in the air chamber between the piston and the disposable tip thus aspirating the fluid. Due to that air cushion certain standard procedures should be followed in order to secure good performance. - Page 7 Residual (Empty) When the aliquots of the dispensing or reverse pipetting operations have been dispensed a small amount of fluid is left in the tip (in order to ensure accurate dispensing of the last aliquot). The removal of the fluid with a blowout is called Residual. Note: when using residual, it is recommended to use a starting point below standard home position equal to the amount used as residual.
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Page 8: Rline Terminology
4 rLine terminology Step Piston movements are defined in steps for all rLine family modules. Positional control of the piston is handled by detecting the angular movement of a 7-segment wheel (Figure 2). The number of steps is converted to linear movement by the screw pitch. Table 1 illustrates the conversion between step and volume in each rLine models. -
Page 9: Drive Cycle
Level sensing, LS Sartorius rLine models 5-200ul LS and 50-1000ul LS integrates capacitive liquid level detection (cLLD) or another word, level sensing (LS). To use that feature, conductive tips must be used to generate detection circuit capacitance change when tip is connected and tip is in touch with liquid. -
Page 10: Technical Specifications
5 Technical Specifications Liquid handling specifications Sartorius rLine liquid handling performance is defined using gravimetric testing with distilled water performed in accordance with ISO 8655. Table 2 illustrates the performance of each rLine models. Table 2. rLine performance in different test volumes... -
Page 11: Repeatability
Repeatability Mechanical and physical variables of the rLine module, air, liquid, tip used and environment will cause small variations in both the amount of liquid aspirated and time taken. Illustrated typical drive profiles are shown in Figure 4. Speed 6 dispense braking Speed 6 aspirate braking Dispense acceleration Aspirate acceleration... -
Page 12: Mechanical
Mechanical Mounting The housing has two holes for two 4 mm mounting screws. Figures 5-6 illustrates the key mechanical dimensions of the rLine models. The weight of the modules is approx. 140 g. Figure 5. rLine models dimensions without tips Figure 6. - Page 13 Locating There are two positioning holes (ø 2,01 ± 0,01 tolerance) as indicated in Figure 7. Another option for locating is the precision machined groove at the bottom of the aluminum base. Figure 7. rLine location guide dimensions Tip pick up force For the rLine models, it is recommended to use specific force to pick up a tip from the tray.
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Page 14: Electrical Specifications
Electrical specifications Operating voltage 9 -12 VDC Required power supply min. 10 VA Current consumption, idle < 100 mA Average current consumption < 500 mA Current consumption, peak Communication protocols RS232 Connector Tyco 609-1427 Serial communication 9600-115200 kbps, 8, 1, none Please notice following: Electronics ground is not in contact with the housing. -
Page 15: Liquid Level Detection (Ls Models Only)
Liquid level detection (LS models only) Liquid level detection is based on a capacitance change. The detection signal is created using an oscillator with a nominal frequency at approx. 50 kHz. From the change in frequency it is possible to detect if the tip is in liquid. However, the sensor is sensitive to all changes in the environment, including the type of the of tip rack, attachment fixtures etc. -
Page 16: Messaging And Initialization
6 Messaging and initialization rLine uses custom, proprietary real-time firmware. Most user-configurable settings must be set after each power-up (such as speed if not default) but some settings can be stored in permanent memory. Firmware upgrade cannot be performed without removing the module and requires special programming tools. -
Page 17: Messaging
Messaging The protocol is intended foremost to be simple with a reasonable measure of reliability. It is based on a synchronous master/slave model in which all communication occurs between the master and a single command rLine. Messages from the master to the rLine are categorized as either a to do something, query or a... -
Page 18: Communicating With The Module
Communicating with the module Communicating with rLine is based on commands and queries. Commands will be acknowledged when received but not on completion. Queries will be answered. Therefore the completion of a command must be checked with a query. Response message structure is identical to send messages. General rLine will respond to every message it receives and recognizes as addressed to it. -
Page 19: Configuration Commands
Configuration commands The following sets of commands are used to configure each module and are usually carried out before installing the module. Set rLine slave address (default: 1) n=1-9 Example: *A2 Response: “ok” or “er1-4” Set RS232 baud rate (default 0) n=0: 9600 bps n=1: 19.2 kbps n=2: 28.8 kbps... -
Page 20: Initialization Commands
Initialization commands After every power-up, each module must be initialized. Run to the Zero position RZ initializes the piston and parameters (position = 0). Response: “ok” or “er1-4” Locates the mechanical lower limit of piston travel and returns the piston to the pre- programmed zero position. -
Page 21: Drive Commands
Drive commands Inward speed (1-6) Selects the aspirating speed. 1 represents the slowest speed and 6 the fastest speed. Drive must be stopped to change the speed. Response: “ok” or “er1-4” Outward speed (1-6) Selects the dispensing speed. 1 represents the slowest speed and 6 the fastest speed. Drive must be stopped to change the speed. -
Page 22: Status Commands
Status commands These queries will only be answered at idle. Display version Return message: nnnn, where nnnn is software version as a string On error: er1-4 Display model Return message: nnnn, where nnnn is rLine model as a string On error: er1-4 Display cycles Return message:... - Page 23 Most common return messages are: no errors, module ready for commands Running + Drive busy, module is active general error. Drive has not successfully completed last command Display errors Displays error registers as a list. Error registers are reset after response. Return message: nnn, where nnn is the error list On error:...
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Page 24: Error Handling
7 Error handling For all recognized error events rLine will respond with an error message. er1, er2, er3 and er4 For all messages there are four possible immediate error responses, “er1” will be sent when the command has not been understood by the module. Example: “RPx200”... -
Page 25: Maintenance
It's recommended to either replace the lower parts or at a minimum lubricate the cylinder seals and screw every 750000 drive cycles or 12 months, whichever comes first. Lubricant used is a specially formulated high-performance grease and should not be substituted without consulting Sartorius. Autoclaving All rLine models are not autoclavable. - Page 26 10 PC Software The easiest way to get connected to rLine and tests it’s features is to use Sartorius rLine Pipette Control PC application. It is standalone application running on Windows 7 and newer platforms. More information about the software can be found from software user guide.
- Page 27 11 Order information rLine models and all accessories can be purchased using the following order codes: rLines Model Order codes / CAT numbers rLine 1-ch 5-200ul LS 710993 rLine 1-ch 50-1000ul LS 710995 rLine 1-ch 100-5000ul 710969 Tips Model Order codes / CAT numbers 5-200ul optifit tips 790200 5-200ul filter tips...
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