NEW BRUNSWICK SCIENTIFIC I-2500KC Manual To Operations
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NEW BRUNSWICK SCIENTIFIC I-2500KC Manual To Operations

Digital refrigerated incubator shaker
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I-2500KC
Digital Refrigerated
Incubator Shaker
with PROGRAMMABLE TIMER
MANUAL No. M1284-1054
Revision B
July 12, 2002
NEW BRUNSWICK SCIENTIFIC CO., INC.
BOX 4005 • 44 TALMADGE ROAD • EDISON, NJ 08818-4005
Telephone: 1-732-287-1200 • 1-800-631-5417
Fax: 732-287-4222 • Telex: 4753012 NBSCO
Internet: http://www.nbsc.com • E-mail: bioinfo@nbsc.com

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  • Page 1 PROGRAMMABLE TIMER MANUAL No. M1284-1054 Revision B July 12, 2002 NEW BRUNSWICK SCIENTIFIC CO., INC. BOX 4005 • 44 TALMADGE ROAD • EDISON, NJ 08818-4005 Telephone: 1-732-287-1200 • 1-800-631-5417 Fax: 732-287-4222 • Telex: 4753012 NBSCO Internet: http://www.nbsc.com • E-mail: bioinfo@nbsc.com...
  • Page 2 I-2500KC • M1284-1054 User’s Guide...
  • Page 3 This equipment must be operated as described in this manual. If operational guidelines are not followed, equipment damage and personal injury can occur. Please read the entire User’s Guide before attempting to use this unit. New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 4 THIS PAGE INTENTIONALLY BLANK I-2500KC • M1284-1054 User’s Guide...
  • Page 5 Reproduction, adaptation, or translation without prior written permission from New Brunswick Scientific is prohibited. Disclaimer Notice New Brunswick Scientific Co., Inc. reserves the right to change information in this document without notice. Updates to information in this document reflect our commitment to continuing product development and improvement.
  • Page 6 THIS PAGE INTENTIONALLY BLANK I-2500KC • M1284-1054 User’s Guide...
  • Page 7 I Series Shaker that has been repaired or altered outside the NBS factory without prior authorization from New Brunswick Scientific. For a period of two years (or more) after the shipment date, the I Series warranty will be in effect as long as the shaker has not been in operation for a total of 10,000 hours.
  • Page 8 THIS PAGE INTENTIONALLY BLANK I-2500KC • M1284-1054 User’s Guide...

  • Page 9: Table Of Contents

    OPERATION ..............................50 ..........................50 TARTING THE HAKER ..........................50 ONTINUOUS .........................51 HECKING ETPOINT ..........................51 ERIES IMER ...........................52 LARM UNCTIONS ......................53 RIMARY EMPERATURE ETPOINT ........................53 ROGRAMMED ........................54 ROGRAMMED HAKE .......................54 EMPERATURE FFSET ALIBRATION 7.10 ..........................55 OTAL UNNING New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 10 ONTROL OARD EPLACEMENT SPECIFICATIONS............................66 REPLACEMENT PARTS & ACCESSORIES .....................68 10.1 ..............68 EPLACEMENT ARTS ESCRIPTIONS AND UMBERS 10.2 & P ..................69 CCESSORY ESCRIPTIONS UMBERS DRAWINGS ..............................72 11.1 ..........................72 ONTROL CHEMATIC 11.2 ...........................78 IST OF RAWINGS INDEX................................80 I-2500KC • M1284-1054 User’s Guide...

  • Page 11: Overview

    The shaker may be operated either continuously or in a timed mode via a programmable timer for shaking periods of 0.1 hour to 99.9 hours. The I-2500KC is equipped with audible and visible alarms that are activated when an alarm condition exists, as follows: •...
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  • Page 13: Inspection & Verification

    Fisher sales representative. Do not discard any of the packing material. Unpacking & Verification Verify against your packing list that you have received the correct materials. Report any errors or missing material to your local Fisher sales representative. New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 14 THIS PAGE INTENTIONALLY BLANK I-2500KC • M1284-1054 User’s Guide...

  • Page 15: Preparing The Location

    Space Requirements The surface where you place the I-2500KC should be smooth, level and sturdy. It is essential that the instrument be situated in an area where there is sufficient space for the shaker and service lines (see Figure 1 below).

  • Page 16: Environment

    Environment The I-2500KC operates properly under the following conditions: 0°C - 35°C, 90% humidity, non-condensing. I-2500KC • M1284-1054 User’s Guide...

  • Page 17: Features

    Figure 2: I-2500KC Front Panel CONTROL ELECTRONIC PANEL KEYPAD TIMER ON/OFF SWITCH FOOT PEDAL New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 18: Keypad

    This key is used to change the displayed parameter. SELECT • These keys are used to adjust the setpoint of a (UP), (DOWN) displayed parameter up or down. They also allow the user to enter the for setpoint SET MODE changes. I-2500KC • M1284-1054 User’s Guide...
  • Page 19 Used to display current incubator temperature and set primary temperature setpoint. • * Used to display current incubator temperature and set alternate or secondary temperature setpoint, which is used with the electronic timer. New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 20: Electronic Timer

    KEY OUTPUT m KEY ON/OFF SWITCH MODE PROG 2 CHECK WRITE SWITCH AUTO AUTO TIME ADJ DRAWER 1 2 MAN TIMER CYCLE/PULSE COPY MODE BOOT PULSE 1 2 AUTO DRAWER COVER (OPEN) COPY CYCLE/PULSE I-2500KC • M1284-1054 User’s Guide...
  • Page 21 KEY. SHIFT KEY Moves the cursor ( ) to specify a day. Allows the user to adjust the time. TIME ADJ WRITE Sets in the specific time in RUN MODE or PROGRAMMING MODE. New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 22: Electronic Timer Display Detail

    When the MODE SWITCH is in the RUN position, this indicates that there are programmed operations for Circuit Indicators PROG1 or PROG2. Indicates the position of the MODE SWITCH when it is in the P1 or P2 position. I-2500KC • M1284-1054 User’s Guide...

  • Page 23: Platform Assemblies

    Platform Assemblies The I-2500KC can be used with a wide variety of NBS 18" x 30" (46 cm x 76 cm) platforms which will accept a variety of clamps for flasks and test tubes. Quick Change Platform Kit This easy-load accessory (NBS #M1193-9931) enables the user to snap-in platforms without tools or hardware.

  • Page 24: Refrigeration System

    Controls the refrigeration system. Vent The I-2500KC is provided with a vent to exhaust gas or allow small amounts of ambient air into the chamber. The vent is located in the left side of the cabinet. When the knob is tight in a clockwise position then the vent holes are sealed.

  • Page 25: Getting Started

    Leveling the Unit The I-2500KC should be installed on a smooth, sturdy surface, and must be leveled before operation. The eight feet can be adjusted for leveling by loosening the locking nuts on the threaded studs attached to the feet and retighten when the level condition is attained.

  • Page 26: Quick Change Platform Option

    It should snap down into place and be retained by the springs. Check that the rear edge of the platform is engaged under the bend of the rear retainer. I-2500KC • M1284-1054 User’s Guide...

  • Page 27: Initial Startup

    Initial Startup Before initially turning on the unit, verify the following: • The lid is in the closed position. • Set the to the position and to the OUTPUT ON/OFF SWITCH PROG1 PROG2 position. New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 28: Electronic Timer Program On/Off Switches

    KEYPAD SELECT KEY is lit. 3. If the word appears in the display, the shaker is 4. If a numeric value appears in the display, press the to stop the START/STOP KEY shaker. I-2500KC • M1284-1054 User’s Guide...

  • Page 29: The Electronic Timer

    The is equipped ELECTRONIC TIMER with a built-in battery that stores the day/time settings in memory when the I-2500KC is turned To change the day/time settings of the , perform the following: ELECTRONIC TIMER 1.

  • Page 30: Rdinary Imer Peration

    8:30 am and stop at 5:15 pm from Monday PROG1 through Friday. To accomplish this, perform the following: 1. Enter the set by setting the in the position. The PROG1 SET MODE MODE SWITCH initial display will appear: PROG1 I-2500KC • M1284-1054 User’s Guide...
  • Page 31 . In this case, the timer will display the time the PROG1 operation will stop. MO N WE D - : - - 8 : 3 0 - : - - New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 32 , and OUTPUT SWITCH AUTO PROG1 PROG2 set the to the position. When the is set to MODE SWITCH MODE SWITCH position, the timer does not operate automatically and the output of the timer turns off. I-2500KC • M1284-1054 User’s Guide...

  • Page 33: Sample Program

    8. If it does not display 37, press the or the until it says 37. 9. Make sure that the is off. HOUR TIMER 10. Press the on the until the SELECT KEY CONTROL PANEL KEYPAD HRS FUNCTION illuminates. INDICATOR New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 34 5. Press until appears. 6. Press the to enter your choices into memory. WRITE KEY 7. Press the once, then press the until the cursor ( ) arrives at SET KEY SHIFT KEY Monday. I-2500KC • M1284-1054 User’s Guide...
  • Page 35 7. Press the once, then press the until the cursor ( ) arrives at SET KEY SHIFT KEY Friday. 8. Press the . A bar will appear in the Friday position. SET KEY New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 36 This is for viewing only. Each time the key is pressed, the pre-set operations are displayed in sequence, beginning with . After scrolling through all the PROG1 operations in , the display will scroll through operations. Finally, PROG1 PROG2 it will return to the real time (current) display. I-2500KC • M1284-1054 User’s Guide...

  • Page 37: Multiday Operation

    8 : 3 0 - : - - - : - - 3. Press the to enter this operation in memory. The timer will display the WRITE KEY next operation to be programmed. New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 38 MO N WE D 8 : 30 0 : 0 0 MO N WE D FR I - : - - - : - - - : - - NOTE: I-2500KC • M1284-1054 User’s Guide...

  • Page 39: Cyclic Operation

    3. Using the , select the day of the week that the cycle operation is SHIFT SET KEYS to start. In this example, the cycle is to be performed on Monday. Using the New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 40 0:00. The timer will display the complete operation for approximately one second, then the display will return to the initial , with the number of programmable operation steps PROG1 SET MODE DISPLAY remaining displayed in the lower right hand corner. I-2500KC • M1284-1054 User’s Guide...

  • Page 41: Viewing Programs

    WRITE KEY necessary keys and press the to enter these values into memory. WRITE KEY Each time the is pressed, the programmed times are displayed in WRITE KEY the order that they were set. New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 42 In the following example, the current day of the week is Wednesday, and the timer operations to be performed on Thursday will be checked. MO N WE D I0 : 3 0 8 : 3 0 5 : l 5 I-2500KC • M1284-1054 User’s Guide...
  • Page 43 SHIFT KEY the Sunday position. In all other days of the week the will be blinking. MO N WE D FR I I0 : 3 0 8 : 30 5 : l 5 New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 44: Day Override

    When you use the day override function, the operation that is transposed to another takes precedence. For example, consider the following control scheme: I-2500KC • M1284-1054 User’s Guide...
  • Page 45 Sunday’s. To transpose Sunday’s operations onto Wednesday and Thursday, do the following: 1. Verify that the timer is in . If it is not, place the RUN MODE MODE SWITCH position. New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 46 4. If no override has been previously set, the following display will appear: MO N WE D 5. Using the , select both Wednesday and Thursday as the SHIFT SET KEYS days to be overridden. MO N WE D I-2500KC • M1284-1054 User’s Guide...
  • Page 47 WE D I0 : 3 0 8 : 3 0 5 : l 5 2. Open the lower drawer of the timer, and hold down the for one COPY KEY second or more. New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 48: Clearing/Canceling Programs

    2. Open the lower drawer of the timer, and press the . The display will CLR KEY revert to the initial , and the number of steps PROG1 SET MODE DISPLAY remaining will be increased by the number of steps cleared in Program 1. I-2500KC • M1284-1054 User’s Guide...
  • Page 49 - : - - - : - - - : - - NOTE: This procedure is identical for . To perform this procedure for PROG2 PROG2 set the in the position. MODE SWITCH New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 50: Operation

    The I-2500KC can be operated in four different modes depending upon the application. The modes incorporate the various features that are unique to the I-2500KC, and are dependent upon timed operation. They are as follows: Control Output On/Off Switch Keypad...

  • Page 51: Checking Any Setpoint

    KEYPAD, I SERIES TIMER MODE be used for timed experiments. 1. Verify that the on the is in OUTPUT ON/OFF SWITCH ELECTRONIC TIMER PROG1 position and in the position for PROG2 New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 52: Alarm Functions

    START/STOP KEY TIME INDICATOR Alarm Functions The I-2500KC is equipped with an audible and visual alarm that is activated at predetermined times. It may be deactivated in the following way: 1. Press until the is illuminated.

  • Page 53: Primary Temperature Setpoint

    3. To reactivate the temperature controller, press the until the desired temperature setpoint is displayed. Programmed Temp Mode This I-2500KC allows a secondary or alternate temperature setpoint to be entered. of the programmable determines whether the I-2500KC PROG1 ELECTRONIC TIMER operates at a primary or alternate/secondary temperature setpoint.

  • Page 54: Programmed Shake Mode

    KEYS 9. Press the to start the shaker. START/STOP KEY NOTE: The I-2500KC can be operated in both PROGRAMMED SHAKE simultaneously. To operate the unit in this mode, PROGRAMMED TEMP MODES follow the procedures for both modes. Set for both...

  • Page 55: Total Running Time

    START/STOP KEY 7.10 Total Running Time The control module of the I-2500KC totals the time the shaker has been to track hours of usage. The total running time of the unit is independent of the keypad function. To display the accumulated running time: New Brunswick Scientific Co., Inc.

  • Page 56: Maint Indicator

    3. Press the 7.12 Power Failure In the event of a power failure, the I-2500KC is equipped with an automatic restart function. If the shaker was in operation prior to the power interruption, the Shaker will begin to operate at its last entered setpoint, and the electronic timer will resume its operation as programmed after the power recovery.
  • Page 57 THIS PAGE INTENTIONALLY BLANK New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 58: Preventive Maintenance

    Every 3 months of usage, the back covers should be removed (after removing the power plug), and the coil and surrounding area should be vacuumed. If the unit is in a dusty environment, this should be done more often. I-2500KC • M1284-1054 User’s Guide...

  • Page 59: Changing Fuses

    One is to protect the control circuitry, and the other is to protect the motor circuit. The third fuse holder, marked "lamp", is not used on the I-2500KC. To remove either fuse, insert a small flat-bladed screwdriver and turn counterclockwise until it disengages and the fuse holder springs free.

  • Page 60: Motor Assembly Replacement

    5. Remove the 2 hex nuts and washers and lift out the motor and plate assembly. 6. Position the new motor assembly back onto the unit. 7. Reinstall the 2 hex nuts with hardware. Do not tighten. I-2500KC • M1284-1054 User’s Guide...

  • Page 61: Esd Precautions

    2. Open the lid and remove the two 3/8 inch hex nuts. These nuts are located on the back side of the front panel. 3. Carefully push the now exposed two threaded studs so that the control panel eases out from the lid. New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 62 8. Remove the two screws that fasten the heat sink to the front panel bracket. 9. Lift the board out of the bracket assembly and disconnect the keypad connector from NOTE: Be careful not to lose the five ¼ inch spacers or the gray insulator I-2500KC • M1284-1054 User’s Guide...

  • Page 63: Temperature Control Board Replacement

    2. Open the lid and remove the two 3/8 inch hex nuts. These nuts are located on the back side of the front panel. 3. Carefully push the now exposed two threaded studs so that the control panel eases out from the lid. New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 64 (keys mate and no pins remain exposed). 10. Reinstall the panel and secure with the two 3/8 inch hex nuts. 11. Connect the line cord to the rear of the shaker. I-2500KC • M1284-1054 User’s Guide...
  • Page 65 THIS PAGE INTENTIONALLY BLANK New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 66: Specifications

    Timer counts down and digital display indicates remaining time. Can be deactivated for continuous operation. Additionally, unit will display total accumulated running time for service information. I-2500KC • M1284-1054 User’s Guide...
  • Page 67 510 lb. (223 kg) Platform Dimensions 18" x 30" (46 cm x 74.6 cm) Inside Chamber Dimensions 34-3/4" (88.3 cm) wide x 22 1/4" (56.6 cm) deep x 19 1/4" (48.9 cm) above the platform. New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 68: Replacement Parts & Accessories

    6" Fan Evaporator M002 P0620-2561 Glass Window M1193-9902 Gas Springs P0640-0380 Fuse Drawer P0380-6640 45 Amp Solid State Relay SSR002 P0400-3151 Condenser M1164-0100 Automatic Expansion Valve P0220-2081 Filter Dryer P0200-1210 Evaporator Coil M1193-9949 Compressor 115V P0620-0811 I-2500KC • M1284-1054 User’s Guide...

  • Page 69: Accessory Descriptions & Part Numbers

    10.2.1 Easy-Load Platform Kit A quick-change platform option is available for the I-2500KC. This easy-load accessory enables the user to snap in platforms without tools or hardware. The kit includes a subplatform with spring clips, an extra counterweight, and hardware.
  • Page 70 NBS flask clamps are used on a variety of shaker platforms. Flat head screws of different lengths and thread pitch are used to secure the clamp. The tables below identify the proper screw for your shaker application by reference to the head style. I-2500KC • M1284-1054 User’s Guide...
  • Page 71 Phillips (+) head aluminum, phenolic screw and stainless steel platforms. 10-32 x 5/16 (7.9 mm) S2117-3050 all stainless steel flat slotted (-) head screw platforms NOTE: 2800 ml Fernbach Flask Clamp applicable to above chart New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 72: Drawings

    11.1 Control Schematic Figure 12: Control Schematic, Overview I-2500KC • M1284-1054 User’s Guide...
  • Page 73 Figure 13: Control Schematic, Quadrant A New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 74 Figure 14: Control Schematic, Quadrant B I-2500KC • M1284-1054 User’s Guide...
  • Page 75 Figure 15: Control Schematic, Quadrant C New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 76 Figure 16: Control Schematic, Quadrant D I-2500KC • M1284-1054 User’s Guide...
  • Page 77 CHARGING CYLINDER. SET PRESSURE ON LOW SIDE TO 27 PSI (+1, -0 PSI). Item NBS Part Number Description Qty/Per P0840-0120 Refrigerant R134A A-150 Sight Glass P0200-1210 Filter/Dryer M1193-9949 Evaporator P0220-2081 Valve, Auto Expansion P0620-0851 Compressor New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 78: List Of Drawings

    Quick Change Platform Installation Electronic Timer Program On/Off Switches Belt Replacement Motor Assembly Replacement Control Board Replacement 61, 63 Control Schematic—Overview Control Schematic—Quadrant A Control Schematic—Quadrant B Control Schematic—Quadrant C Control Schematic—Quadrant D Refrigeration Schematic I-2500KC • M1284-1054 User’s Guide...
  • Page 79 THIS PAGE INTENTIONALLY BLANK New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 80: Index

    LED Display · 18 Cyclic Operation · 39 Status Indicators · 19 Day Override · 43, 46 User Interface Keys · 18 Day/Time Settings · 29 Keypad Timer Display Features · 22 Cancelling · 51 Features · 21 I-2500KC • M1284-1054 User’s Guide...
  • Page 81 Power Failure · 55 Visual Alarm Programmed Shake Mode · 53 Disabling · 51 Programmed Temp. Mode · 52 WARNING Quick Change Platform · 68 Symbol for · 5 Installation · 26, 27 Warranty · 7 New Brunswick Scientific Co., Inc. User’s Guide...

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