Important user information Reading this entire manual is necessary for full under- standing and safe use of this product. Should you have any comments on this manual, we will be pleased to receive them at: Pharmacia Biotech AB S-75182 Uppsala Sweden Pharmacia Biotech reserves the right to make changes in the specifications...
1. Introduction MultiTemp external electrophoresis equipment. The liquid temperature range is -10 to +90 °C. The unit contains KLEA 134a*, an ozone friendly HFC-type coolant. MultiTemp III both supplies coolant for high voltage techni- ques and provides temperature control at higher temperature (e.g.
7. Do not use the circulator beyond its specifications or for other than its intended use. 8. Do not touch the stainless steel bath cover and tubings when the unit is used at a high working temperature. Under these conditions, the surface temperature can rise up to approximately 70 °C.
1. Introduction and 2. Safety information General cut-off 2.3 Safety The heater, the compressor and the circulation pump can be features switched off by a relay built into the unit. This general cut- off will be activated if a malfunction is detected by the safety sensors.
3. Installation 1. Check the contents against the packing list supplied. Inspect 3.1 Unpacking for any damage that may have occurred during transit. Report any damage immediately to your local Pharmacia Biotech installation representative and to the transport company concerned. 2.
Silicone tubing (inner diameter 8 mm) can be used over the 3.2. Tubing entire working temperature range of the unit. PVC tubing connec- can only be used in the temperature range of 10 to 60 °C. tions 1. Connect the inlet and outlet tubing to the nozzles on the rear of the circulator (Fig.
3. Installation liquid pour into a large vessel using hydrostatic pressure. The bath contains 3 litres of liquid, plus the volume in tubing and connected equipment. The last millilitres can be removed by opening the screw on the front of the unit, (see Fig. 1). Warning! Do not drain hot water.
4. Operation 4.1 Start-up Fig. 2. Front panel of MultiTemp III. 1. Mode selector 2. Set value down 3. Set value up 4. Enter 5. Reset Before switching on, confirm that the outlet and inlet nozzles are either firmly connected to external equipment or to each other and that the tubing is secured with hose clamps.
the external system and the level in the bath will fall. Add more liquid until the level is once again 2 cm below the top plate. If the safety circuit is triggered, please top up with more liqu- id and restart the unit using necessary.
2. Use rature. The factory pre-set value is 90.0 °C. 3. Confirm with al temperature value. Note: The new value is not saved until sed. 1. Press 4.4 Tempera- ture setting 2. Use 3. Confirm with returns to the actual temperature value. Note: The new set value is not saved until pressed.
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4. Operation 5. Confirm with before the display returns to the actual temperature value. Note: The new value is not saved until has been pres- sed.
5. Trouble shooting 5.1 Error messages Message on display Cause The bath temperature has exceeded the safety temperature limit. Too low water level. The winding of the pump motor is too hot. The temperature sensor is broken. The bath liquid has cooled down below the temperature limit.
6. Maintenance 6. Maintenance Warning! Always disconnect MultiTemp III from the mains 6.1 General (Pull out the mains plug) before maintenance and/or service. Service should be carried out by authorised personnel only. To maintain efficient operation of the compressor cooling unit, have the air grid and liquifier (heat exchanger) checked and cleaned by service personnel two to four times a year, depending on the amount of dust in the air.
The circuit breaker (thermal fuses) for MultiTemp III are loca- 6.2 Fuses ted on the rear of the unit. Note: If the circuit breaker has been triggered, there may be Ratings for the circuit breaker: for 198 to 253 V version for 95 to 132 V version Fuses for the electronics located inside the unit must be repla- ced by authorised personnel only:...
7. Detailed Description MultiTemp III regulates temperature by balancing the effects 7.1 General of constant cooling and controlled pulses of heating. operating Constant cooling is provided by a built-in unit that includes a principle compressor, an air cooled liquifier and an evaporator (a coo- ling coil as heat exchanger) located in the chamber.
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Mains Switch. Starts the cooling compressor and the pump motor that circulates the temperature controlled liquid. Activates the heater if the temperature set is greater than the actual temperature. Change the temperature value. Values are changed in an accelerating manner when either key is held down. Changes between showing the actual temperature and the various program modes.
General cut-off 7.4 Description The heater, the compressor and the circulation pump can be of safety switched off by a relay built into the unit. This general cut- features off will be activated if a malfunction is detected by the safety sensors.
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Accurate Setting of Temperature Limit If a more accurate temperature limit is required, for example 50 °C, follow the procedure below. Set the temperature limit switch to its highest value. Set the working temperature to 50 °C according to Section 4.4. Run the unit and wait until it reaches exactly 50 °C.
Note: Although this feature activates the general cut-off, it is Restart after power failure MultiTemp III automatically restarts after a power failure. 7.5 Manual Make sure that this automatic restart will not cause a dange- restart in rous situation to arise. operation The unit can be restarted manually instead by pressing mode 8...
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To start MultiTemp III in 8 mode ON, follow the instructions below: Check that all tubings are firmly connected and secured with hose clamps. Switch the power on with the mains switch. The display flashes Press for start. The version number of the software is briefly shown on the display.
8. Technical Technical specifications (ambient temperature 20 °C) Working temperature range Temperature control constancy at 20 °C Range of temperature limit switch Heater capacity, Cooling capacity, Cooling capacity, Compressor coolant Bath volume Bath opening/depth Pump capacity, pressure, max. Pump capacity, flow rate, max. Noise level at 1 m in front of the unit Mains requirements Power consumption...
9. Ordering information Designation MultiTemp III 100-120 V MultiTemp III 220-240 V Accessories Item Cooling Tubing, 8/12 mm, silicone Tubing Connector Set female and male Hose clamps Insulation for cooling tubing 14/27 mm 3-way Valve Set Code No. 18-1102-77 18-1102-78 Quantity Code No.
Short instructions Note: The following short instructions are intended as a guide to users who are fully familiar with safety procedures and operating instructions described earlier in this manual. Check to make sure that all tubings are firmly connected and secured with hose clamps.