Harvard Apparatus Pump 33 DDS User Manual
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Harvard Apparatus Pump 33 DDS User Manual

Dual drive system
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Pump 33 DDS
Dual Drive System
User's Manual
Pump 33 DDS
70-3333
Publication 5419-013 REV1.0

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Summary of Contents for Harvard Apparatus Pump 33 DDS

  • Page 1 Pump 33 DDS Dual Drive System User’s Manual Pump 33 DDS 70-3333 Publication 5419-013 REV1.0...

  • Page 2: Table Of Contents

    Pump Chain Commands Technical Specifications Command Conventions and Error Messages 40-41 Operating Humidity System Commands Setting Up the Pump 33 DDS (Dual Drive System) Syringe Commands Physical Overview Run Commands Syringe Loading Rate Commands Navigating the Pump 33 DDS Graphical...

  • Page 3: Warranty & Repair Information

    If practical, enclose a sample part or sketch. We offer Warranty a complete reconditioning service. Harvard Apparatus warranties this instrument for a period of two Serial Numbers years from date of purchase. At its option, Harvard Apparatus will repair or replace the unit if it is found to be defective as to All inquiries concerning our products should refer to the serial workmanship or materials.

  • Page 4: Safety Information

    Do not use the equipment in an explosive environment. and make sure line cord is certified for country of use. The operating voltage range for the Pump 33 DDS is 100-240 VAC, 50-60 Hz. OBSERVE ALL WARNING LABELS ON PRODUCT GROUND THE PRODUCT Read all labels on product to ensure proper usage.

  • Page 5: Product Overview- Theory Of Operation

    Any (mode Infuse/Withdraw, syringe 0.5 µl - 60 ml dependent) The Pump 33 DDS motors generate up to 70 lbs of linear Withdraw/Infuse capability force in operation. This force is sufficient to damage delicate, low-volume syringes, as well as many standard...

  • Page 6: Power Connections And Pump Startup

    1. Please ensure that the power switch is in the off position as shown in the Pump 33 DDS Rear View Connections graphic. 2. Plug the power cord in to line voltage. The Pump 33 DDS has a DC power input connector on the rear of the unit. Plug the barrel connector into the DC Input on the rear of the unit.

  • Page 7: Technical Specifications

    Certifications WEEE Modes of Operation: Twin Both syringes of the same size operate *NOTE: Some larger syringes may be compatible with the Pump 33 DDS. Condition identically (flow rate, direction & volume) Please contact Technical Support for more information. Independent...

  • Page 8: Operating Humidity

    Operating Humidity The following is a chart of operating humidity versus temperature for the Pump 33 DDS. Please note as operating temperature increases, the maximum allowable operating humidity decreases. Operating Humidity vs. Temperature Chart Publication 5419-013 REV1.0...

  • Page 9: Setting Up The Pump 33 Dds (Dual Drive System)

    Syringe Loading The Pump 33 DDS syringe mechanisms accommodate syringe sizes 0.5 ul to 60 ml. The pump includes brackets and clamps to hold different sizes and types of syringes for various applications. The following uses P1 as the mechanism example. Both syringe pump mechanisms operate in the same manner.

  • Page 10: Syringe Loading

    Pump Channel 2 (P2) High Force Pump Syringe Clamp Channel 1 (P1) Example Low Force Syringe Clamp Example Pump 33 DDS Showing Syringes Installed with Low Force (P1) and High Force (P2) Syringe Clamps Publication 5419-013 REV1.0...

  • Page 11: Navigating The Pump 33 Dds Graphical

    Navigating the Pump 33 DDS Graphical User Interface The Pump 33 DDS employs a button enabled Graphical User Then the unit will display the Condition Select Screen. If the interface controlled with a large 7” LCD Color Touchscreen display for Condition Select screen does not appear, please press the back quick and easy setup.

  • Page 12: Graphical User Interface Icon Glossary

    Graphical User Interface Button Glossary The following buttons are part of the Pump 33 DDS (Dual Drive System) graphical user interface. Rate Select – Sets Flow Rate Mode Select – Sets Pump Channel Mode Syringe Select – Sets Syringe Type & Size Target Select –...
  • Page 13 Graphical User Interface Button Glossary Minimum Value Allowed – Used to select the minimum value allowed for the parameter entry. Maximum Value Allowed – Used to select the maximum value allowed for the parameter entry. Accept – Used to accept the parameters/settings on a screen and advance to the next menu.

  • Page 14: Independent Condition Setup

    Independent Condition Setup Independent Condition allows the Pump 33 DDS to operate as two separate syringe pumps named P1 & P2. P1 is syringe position 1 (Channel 1), closest to the touch screen interface and P2 is syringe position 2 (Channel 2) and is toward the backside of the unit.
  • Page 15 Independent Condition Setup The bottom of the screen contains the Run, Fast Forward / Fast Reverse and System settings buttons. This manual addresses the Run functions after each condition setup section. The Settings section of this manual covers the system setting functions. Independent Condition Setup Screen The following is a summary of the Independent Condition Setup Screen with detailed explanation of each button function.
  • Page 16 Withdraw Only - The syringe will operate in withdraw mode only. Facing the Pump 33 DDS, the pusher block will pull the syringe plunger from right to left at the set flow rate and volume/time.
  • Page 17 Independent Condition Setup 3) RATE SELECT – Select the flow rate for Pump Channel 1 & Pump The Pump 33 DDS also supports syringes not specifically listed in the syringe table through the Custom Syringe Selection. Select Custom Channel 2. Independent Condition requires the user to enter a flow rate for the selected mode on both P1 &...
  • Page 18 Independent Condition Setup Units - Set the units for the desired flow rate by touching the units found on the right of the number entry box. The following Select Units popup box will appear. Select the volume units from the left hand side of the Select Units popup and time units from the right hand side.
  • Page 19 Independent Condition Setup Select the Target Time entry box to enter the target time. The time can be entered in HH:MM:SS or sec. The user can toggle between HH:MM:SS or sec by pressing the time units to the right of the target time entry box.
  • Page 20 Independent Condition Setup Pump Channel 1 (P1) Fast Forward Feature Pump Channel 2 (P2) Fast Forward Feature Pump Channel 1 (P1) & 2 (P2) Fast Forward Feature Pump Channel 1 (P1) Fast Reverse Feature Pump Channel 2 (P2) Fast Reverse Feature Pump Channel 1 (P1) &...

  • Page 21: Independent Condition Run

    This operation can be performed from the setup or run screens. Refer to the setup screen button guide shown below (defined at the start of this section). To start the Pump 33 DDS from the setup screen press the Pump Channel 1 (P1) Run/Stop button (5) to start P1 only.
  • Page 22 Press again to unlock using the set password. 21) RUN/STOP PUMP CHANNELS 1 (P1) and 2 (P2) – Press to When the Pump 33 DDS screen is locked, all setting buttons will be start/stop both P1 and P2 at the same time.

  • Page 23: Reciprocating Condition Setup

    P1 will infuse first while P2 is withdrawing. Facing Select “Reciprocating” from the Condition Select Screen. the Pump 33 DDS, the pusher block for the infusing pump channel will push the syringe plunger from left to right at the...

  • Page 24: Reciprocating Condition Run

    Reciprocating Condition Setup 2) SYRINGE SELECT - Select the syringe size and type for Pump Channel 1 (P1) & Pump Channel 2 (P2). The Syringe Select Methods for Reciprocating Condition is similar to Independent Condition. Since both P1 and P2 are the same size in Reciprocating Condition, the user only needs to set the syringe for P1 and the syringe for P2 will set automatically.

  • Page 25: Twin Condition Setup

    The volume or time of the cycle is set Select for the P1 Pump Channel and the P2 Pump Channel will be in the Target Select selection. Facing the Pump 33 DDS, the automatically set. The second difference is the syringe ganging P1 and P2 pusher blocks will pull the syringe plungers from feature, which will be discussed under SYRINGE SELECT, below.
  • Page 26 Twin Condition Setup The x1 selection allows both syringes to operate as two identical 4) TARGET SELECT – Select the Target Volume or Time depending on individual syringes with the same mode, syringe, flow rate and target the Mode selected. The Infuse, Withdraw, Infuse/Withdraw or volume or time.
  • Page 27 Twin Condition Setup The Rate Select screen for the x1 Gang selection will look the When the user runs the setup for this example the Twin Run screen will display the pump status along with the set flow rate same as the Rate Select screen for both Independent and and target volume.
  • Page 28 Twin Condition Setup Example x2 Ganging: The user wants both 2.5ml syringes to infuse at the same rate and volume. The syringe outputs will need to be connected together by the user. In this case, the user will select the Gang x2 toggle selection located in the top right hand corner of the Select Syringe menu along with the proper 2.5 ml syringe.

  • Page 29: Twin Condition Run

    Twin Condition Run The Twin Condition Run screen is similar to both the Independent and Reciprocating Run screens. Note: The user needs to select and highlight both P1 and P2 to move both P1 and P2 pusher blocks at the same time using the Fast Forward and Fast Reverse buttons. Please see below for a summary of the Twin Condition Run screen.

  • Page 30: Settings

    Settings Menu Please see the Independent Condition Run section of this manual The Pump 33 DDS Settings menu controls basic system operations. for instructions on setting the Screen Lock/Unlock. The instructions This section describes the options available in this Settings screen of apply to all three operating conditions (Independent, Reciprocating the application software.

  • Page 31: Setting Time/Date

    Setting Time/Date The Pump 33 DDS displays the date and time on each screen in the user interface. Built-in battery backups maintain the date and time even if the pump loses power or is unplugged for a period of time.

  • Page 32: Pump Address

    Set Password Screen Baud Rate For attaching the Pump 33 DDS to an external device using RS- 232 serial communications, you can easily adjust the baud rate to optimize communications. TO ADJUST THE PUMP BAUD RATE: 1.

  • Page 33: Force Setting

    Force Setting The Pump 33 DDS Pump Channel 1 (P1) and Pump Channel 2 (P2) motors generate up to 70 lbs of linear force per syringe in operation. This force is sufficient to damage delicate, low-volume syringes, as well as many standard syringes if pumping viscous fluids.

  • Page 34: Alarms

    USB Pinout USB Virtual Driver Installation When you connect the Pump 33 DDS to a Windows computer for the first time, a driver will need to be installed for the Windows PC to communicate with the Pump 33 DDS. The following section gives an overview on the installation of the Virtual CommPort Driver supplied with the Pump 33 DDS using the Windows 7 operating system.

  • Page 35: Usb Virtual Commport Driver Installation

    The following section gives an overview on the installation of the Virtual CommPort Driver supplied with the Pump 33 DDS. When you connect the Pump 33 DDS to a Windows computer for the first time, the installation of a USB driver is required on the Windows PC for it to communicate with the Pump 33 DDS.
  • Page 36 3. Windows security may prompt you to authorize the installation of the driver. Enable the check box to the left of the text ’Always trust software from “Harvard Bioscience”.’ and click the “Install” button. 4. Once the Pump 33 DDS Windows driver installs, click the Finish button to close the installer. Publication 5419-013 REV1.0...

  • Page 37: Upgrade Software

    There are two components of the pump software: the Boot Loader, which controls how the system manages updates, and the main Application. Harvard Apparatus may occasionally provide updates to each portion of the software, either individually or as a pair, to add new features and/or improve functionality.
  • Page 38 External Connections 7. Click the Start Update button (see below). 8. After the update is complete, press Reboot to power cycle the pump (see below). Publication 5419-013 REV1.0...

  • Page 39: Pinout

    External Connections RS-232 Pinout Baud Rates: 9600 19200 38400 57600 115200 Word size – 8 Pin # Signal Parity – None Stop Bits – 1 Pump 1, 4, 6-9 RS-485 Pinout CAUTION: Do not connect to firewire ports on a PC. Damage may occur to pump and/or PC.

  • Page 40: Input/Output Connectors

    External Connections Input/Output Connectors The Pump 33 DDS includes two input/output (I/O) All Inputs are pulled high to +5 Vdc through a 10k resistor connectors. There is one for Pump Channel 1 (P1) All Outputs are at TTL Logic Levels. Pulse duration should and Pump Channel 2 (P2).

  • Page 41: Pump Chain Commands

    If the pump is in Poll ON mode, an XON character is added after a single USB or RS-232 interface on a computer. the prompt. Pump 33 DDS commands are communicated to the pump via the In the following list of commands: USB or RS-232 port interface through a terminal program. In using the Pump Chain commands, you will need to assign each pump in •...

  • Page 42: System Commands

    Pump Chain Commands ARGUMENT ERRORS SYSTEM COMMANDS Argument errors are displayed when a command argument ADDRESS is unrecognized or missing. The argument in question will be displayed except in the case of missing arguments. Sets or displays the pump address. Valid range is 0 to 99. The argument error has the following format: Command format: <lf>[<addr>]Argument error: [bad argument]...
  • Page 43 Pump Chain Commands BRIGHT ECHO Sets or displays the backlight brightness level in percent. Valid Sets or displays the RS232/USB echo state. Valid states are on range is 0 to 100. Note that specifying 0 will turn the backlight or off. off so that the screen will not be visible.
  • Page 44 Pump Chain Commands FORCE FTSWITCH Sets or displays the infusion force level in percent. Valid range Sets or displays the footswitch setting. The footswitch may be is 1 to 100. used in a toggle on or off mode (momentary), a press to run mode (falling), or a release to run mode (rising).
  • Page 45 Pump Chain Commands POLL TIME Sets or displays the date and time. Sets or displays the polling mode state. When polling mode is off, prompts are displayed when an event Command format: time[<sp>{mm/dd/yy|dd/mm/yy|yy/mm/dd}{<sp>} happens, such as a target being reached. {hh:mm:ss}] When polling mode is on, prompts are not displayed when an event happens, and an XON character is output when the pump is...
  • Page 46 If the Command format: {on|off} parameter is omitted, the current On/Off setting is returned. Query response: Command format: touch[<sp>{on|off}] <lf>[<addr>]Pump 33 DDS #.#.# <lf>[<addr>]<prompt> Query response for “touch”: VERBOSE <lf>[<addr>]{On|Off} <lf>[<addr>]<prompt> Specify the level of detail displayed in error messages.

  • Page 47: Syringe Commands

    Pump Chain Commands SYRINGE COMMANDS Command format (Independent Condition): syr<sp>{a|b|ab}[<sp>[?|<code>[<sp>{?|index}]]] Example 1: syr<sp>ab Sets or displays the selected syringe setting for one or both axes. Query response to syr<sp>{a|b|ab}: Quick start only. Valid 3-character manufacturer codes are shown (Show syringe that has been selected for axis a, or b, or list in the table below.
  • Page 48 Pump Chain Commands SVOLUME DIAMETER Sets or displays syringe volume. Sets or displays the syringe diameter in mm. Command format (Independent Condition): Command format (Independent Condition): svolume<sp>{a|b|ab}[<sp>{syringe volume} {ul|ml}] diameter<sp>{a|b|ab}[<sp>{syringe diameter}] Query response to svolume<sp>{a|b|ab} Query response to diameter<sp>{a|b|ab}: <lf>[<addr>]<axis># xl<cr> (displays one line per requested axis) <lf>[<addr>]<axis><sp># (displays one line per requested axis) <lf>[<addr>]<prompt>...

  • Page 49: Run Commands

    Pump Chain Commands RUN COMMANDS RATE COMMANDS IRUN NOTE: In the descriptions for Rate commands, the text “xl/xxx” is used as a placeholder to indicate the actual flow rates. For Runs the pump(s) in the infuse direction if the current commands requiring rate units, use the following format: settings allow.
  • Page 50 Pump Chain Commands IRATE ITRATE Sets or displays the infusion rate. The rate argument may be Sets or displays the target infusion rates for ramping. The rate argument may be replaced with “max” or “min” to set the replaced with “max” or “min” to set the rate to the maximum or minimum rate, respectively.
  • Page 51 Pump Chain Commands RATE TRATE Sets or displays the rate for the current direction (infuse or Sets or displays the target infusion rates for ramping for the withdraw) for the specified axis. It can be used in place of current direction for the specified axis. It can be used in place “IRATE”...
  • Page 52 Pump Chain Commands WRATE WTRATE Sets or displays the withdraw rate. The rate argument may be Sets or displays the target withdraw rates for ramping. The rate replaced with “max” or “min” to set the rate to the maximum argument may be replaced with “max” or “min” to set the rate to or minimum rate, respectively.

  • Page 53: Volume Commands

    Pump Chain Commands VOLUME COMMANDS WVOLUME Displays the withdrawn volume. Command format (Independent Condition): CIVOLUME wvolume<sp>{a|b|ab} Clears the infused volume. Query response: Command format (Independent Condition): <lf>[<addr>]<axis> # xl civolume<sp>{a|b|ab} (displays one line per requested axis) <lf>[<addr>]<prompt> Command format (Reciprocating or Twin Condition): civolume Command format (Reciprocating or Twin Condition): wvolume...

  • Page 54: Time Commands

    Pump Chain Commands TIME COMMANDS TTIME Sets or displays the target time. CITIME Command format (Independent Condition): ttime[<sp>{a|b|ab}<sp>{target time}] Clears the infused time. Query response to ttime<sp>{a|b|ab}: Command format (Independent Condition): <lf>[<addr>]<axis> Target time not set citime<sp>{a|b|ab} Command format (Reciprocating or Twin Condition): citime <lf>[<addr>]<axis>...

  • Page 55: Digital I/O Commands

    Pump Chain Commands DIGITAL I/O COMMANDS INTERNAL COMMANDS INPUT STATUS Reads and displays the trigger input port status. Displays the raw status for use with a controlling computer Command format (Independent Condition): Command format (Independent Condition: input[<sp>{a|b|ab}] status Query Response: Query response to “input<sp>{a|b|ab}”: <lf>[<addr>]<axis><sp>Low|High <lf>[<addr>]<RATEA><sp><TIMEA><sp><VOLA><sp><MDA><LA><SA><IA><DPA><TA>...

  • Page 56: Maintenance, Battery Replacement

    Maintenance, Battery Replacement, and Troubleshooting Maintenance Pump 33 DDS series pumps require no special maintenance other To clean the exterior surfaces, use a lint-free cloth to remove loose than keeping them clean by avoiding accidental spills of pumped dust. Use care to avoid scratching the clear display window. For material.

  • Page 57: Valve Boxes

    Harvard Apparatus offers Continuous Flow and Auto-Fill valve For additional technical details, please contact Technical Support boxes as accessories to the Pump 33 DDS. The Continuous Flow at the email address support@hbiosci.com to request the Harvard valve box is compatible with Reciprocating Condition while the Apparatus Valve Box manual.

  • Page 58: Syringe Manufacturer Volume/Diameter

    Syringe Manufacturer Volume/Diameter Reference Table Air–Tite CMA Sherwood–Monoject Hoshi HSW Norm-Ject Microsyringes Plastic Size Diameter Size Diameter Size Diameter Size Diameter 1 ml 4.69 mm 1 ml 4.608 mm 1 ml 6.50 mm 1 ml 4.674 mm 9.65 7.285 8.865 12.45 10.3 12.6 15.9 14.567 12.6 15.621 Hamilton 20.05 15.1 20.142 700 Series...

  • Page 59: Nominal Flow Rates For Syringe Size

    82.03 nl/min 85.1 ml/min Ordering Information Ordering Information Order # Product 70-3333 Pump 33 DDS (Dual Drive Syringe Pump) Accessories 70-2215 Footswitch (with Phono Plug) 70-4000 RS-485 Cable for Pump-to-Pump Communication, 0.5m 70-4001 RS-485 Cable for Pump-to-Pump Communication, 2.0m 70-4020 RS-485 Cable for Pump-to-Pump Communication, 9.0m...

  • Page 60: Contact Information

    E-mail support@hbiosci.com www.harvardapparatus.com Spain Panlab S.L.U. Canada C/ Energia, 112 Harvard Apparatus, Canada 08940 Cornellà (Barcelona), Spain 6010 Vanden Abeele Street Phone +46 8 470 10 00 Saint Laurent, Quebec, H4S 1R9 +46 8 470 10 50 Phone (514) 335-0792 E-mail info@panlab.com...

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