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Summary of Contents for Invitrogen iPrep
- Page 1 ™ iPrep Purification Instrument For automated purification of nucleic acids using magnetic beads Catalog no. IS-10000 Version B 12 January 2007 25-0927 User Manual...
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Page 3: Table Of Contents
Methods ........................13 General Guidelines.............................13 Getting Started ............................15 ™ Using the iPrep Purification Instrument....................24 Performing Instrument Tests ........................34 ™ Cleaning and Maintaining the iPrep Instrument.................37 Troubleshooting............................39 ™ iPrep Instrument Error Codes ........................42 Appendix........................44 Replacing the Fuse .............................44 Technical Support............................45... -
Page 5: Iprep ™ Experienced Users Procedure
Protocol Card in the slot in the correct orientation (arrow on the ™ card is at the top and card label is facing your left side). Close the iPrep Card Slot. 4. Using the Power Switch located on the left side of the instrument, turn ON the instrument. - Page 6 Discard the used cartridges, tips, and tubes into biohazard waste. Do not reuse the cartridges. ™ To purify more samples using the same iPrep Card, load the racks with new cartridges, tips, tubes, and samples, and start the protocol as described above.
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Page 7: Product Contents
Fuse (6.3 A and 3.15 A) 1 each Instruction Manual ™ See page 5 for specifications and description of the iPrep Purification Instrument, and page 15 to install the instrument. Upon Receiving Examine the unit carefully for any damage incurred during transit. Any damage the Instrument claims must be filed with the carrier. -
Page 8: Safety Information
Safety Information Safety ™ Follow the instructions in this section to ensure safe operation of the iPrep ™ Purification Instrument. The iPrep Purification Instrument is designed to meet EN61010-1 Safety Standards. To ensure safe, reliable operation, always operate ™ the iPrep Purification Instrument according to the instructions in this manual. -
Page 9: Product Specifications
The iPrep Purification Instrument including the Bottom Tray, Cartridge Rack, ™ Tip and Tube Rack is compatible with the iPrep reagents and standard nonhazardous laboratory reagents. *The listed temperature is for the heating block and may not reflect the actual temperature of the sample/solution in a tube. - Page 10 The protection provided by the instrument may be impaired if the instrument is used in a manner not specified by Invitrogen. The UL mark indicates certification by Underwriters Laboratories, Inc and certification by Underwriters Laboratories for Canada—a testing facility...
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Page 11: Unpacking The Iprep Purification Instrument
™ Unpacking the iPrep Purification Instrument Unpacking ™ Follow the instructions below to unpack the iPrep Purification Instrument. The Instructions weight of the instrument is 113 pounds (51 kg). 1. Cut plastic tapes and remove and outer top cardboard. 2. Remove the packages containing the plastic disposables and instrument accessories. - Page 12 Follow the instructions below to remove the protectors added to the instrument Protectors to prevent any damage to the instrument components during transportation. ™ 1. Remove the adhesive tapes near the door (see figure below) and the iPrep Card Slot. ™ 2. Open the iPrep Door as described on page 6 to remove the platform stopper by removing the 3 screws using a small Philips head screwdriver.
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Page 13: Iprep ™ Purification Instrument
™ iPrep Digital Door Display Card Inlet Rear and Side ™ The rear and side view showing various parts of the iPrep Purification ™ View of iPrep Instrument are shown below. Instrument Rear View Side View Fuse Power... - Page 14 ™ iPrep Purification Instrument, Continued User Interface of ™ The user interface of the iPrep Purification Instrument is used to operate the ™ iPrep Instrument instrument and perform maintenance/service by a service engineer, and consists of: • The Digital Display that shows the protocol that is in use and various steps of the protocol (see next page for details).
- Page 15 ™ iPrep Purification Instrument, Continued Digital Display The digital display consists of 4 lines with 20 columns of each line. For the Main menu, Test menu, and Manual menu: • The first line shows the current level • The second and third line show the executable commands on this level •...
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Page 16: Accessory Products
Accessory Products ™ iPrep Spare Parts ™ The following iPrep Spare Parts are available separately from Invitrogen. For more information, visit www.invitrogen.com or contact Technical Support (page 45). Product Quantity Catalog no. ™ iPrep Tip and Tube Rack IS-10101 ™... -
Page 17: Introduction
13 samples (12 samples + 1 positive control) at a time using magnetic bead based technology. The instrument is a simple, user friendly unit with small footprint and is controlled by the iPrep ™ Protocol Card. See page 5 for details. - Page 18 Kits. ™ ™ 2. Insert the appropriate iPrep Protocol Card into the card slot on the iPrep Instrument to start the purification protocol. 3. Assemble the reaction cartridges containing the purification reagents, and ™ the iPrep Tips and Tubes on the racks.
- Page 19 Overview, Continued Features ™ Important features of the iPrep Purification System are listed below: • ™ User-friendly iPrep Instrument design with an integrated heat block and magnetic unit for fast, automated nucleic acid purification within 20-30 minutes • Ability to perform simultaneous purification from up to 12 samples and 1 positive control •...
- Page 20 Purpose of the This manual provides the following information: Manual • ™ Details and specifications on the iPrep Purification Instrument and ™ iPrep Kits • ™ Unpacking and installing the iPrep Purification Instrument •...
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Page 21: Description Of Parts
A pre-programmed iPrep Protocol Card controls the purification parameters ™ such as buffer volumes, mixing steps, and incubation time. The iPrep Instrument is designed to run for 8-10 hours continuously without any cooling period required between runs. See page xiii for a front and rear view of the device. - Page 22 Tip and Tube Rack ™ ™ The iPrep Tip and Tube Rack allows easy placement of iPrep Tips, Sample tubes with samples and Elution tubes to collect the eluate. See page 8 for details. User Interface The User Interface is located on the front of the instrument and contains the digital display, light LED, and keypad.
- Page 23 Protocol ™ ™ The iPrep Protocol Card is a flash, 512 KB memory card. Each iPrep Protocol Card Card is pre-programmed with the purification protocol that directs the volume of reagents used, mixing steps, and incubation time. ™ The following iPrep Protocol Cards are available from Invitrogen (page xvi): •...
- Page 24 ™ ™ Important: The iPrep Cartridge Rack is designed to hold iPrep Reagent Cartridges ™ only. Do not load reagent cartridges from other manufacturer’s on the iPrep Cartridge Rack. ™ iPrep Tip and The iPrep ™...
- Page 25 Description of Parts, Continued Barcode Reader ™ A hand held barcode reader is included with the iPrep Instrument. The Barcode reader allows you to read the barcode on sample and elution tubes to allow you to track samples. The barcode reader is designed to read most standard barcodes including EAN, Code 39, and Code 128.
- Page 26 5.9 inches (l) x 1.2 inches (w) x 0.7 inches (d) ™ iPrep Tips and ™ ™ The iPrep Tips and Tip Holders are included with the iPrep Kits and are Holders ™ placed on the iPrep Tip and Tube Rack as described on page 26. While ™...
- Page 27 ™ iPrep Tubes ™ ™ Two sets of iPrep Tubes are required for the purification protocol. The iPrep ™ sample and elution tubes are included with each iPrep Kit and placed in their ™ respective positions on the iPrep Tip and Tube Rack as described on page 26.
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Page 28: Experimental Overview
Experimental Overview Experimental ™ The iPrep Purification Instrument performs nucleic acid purification as Outline described below. Sample Lysis Sample Lysis DNA binds to magnetic ® DNA binds to ChargeSwitch Magnetic beads at pH <6.0 beads Magnetic separation of beads in tips... -
Page 29: Methods
Kits available from Invitrogen that are compatible for use ™ iPrep Kits ™ with the iPrep Purification Instrument. Ordering information for the kits is on page xvi. Note: Do not use any other kits with the iPrep ™ Instrument. ™ iPrep Sample Type Purification Protocol Time ™... - Page 30 To pause or stop the protocol, see page 32. • ™ ™ Always load the iPrep Cartridge Rack first followed by the iPrep Tip and Tube Rack as described on page 25. Changing the order of loading the ™ iPrep Racks may cause the instrument to stop during a protocol run.
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Page 31: Getting Started
™ ™ Do not start the iPrep Instrument until the iPrep Protocol Card is inserted ™ into the instrument. See next page for details on using the iPrep Card. Important ™ Using the iPrep ™ If you are using the iPrep... - Page 32 Protocol Card in the slot in the correct orientation (arrow on the card is at the top and card label is facing your left side) as shown below. ™ 4. Ensure that card is fully inserted before closing the iPrep Slot and turning the instrument ON.
- Page 33 Getting Started, Continued Using the Setup The Setup screen allows you to set the date and time on the display. Various Screen options are displayed on the screen as shown below that allows you to set the date and time. To return to the previous screen, press ESC. 1.
- Page 34 Getting Started, Continued Date Setup 1. Perform the following steps to set the desired date. The cursor is at SMN or move the cursor to SMN as described on the previous page. Setup>Sys>>Date Cur:CMN CD, CYYY Set:SMN SD, SYYY Key:Up,Dn,RET,ESC 2.
- Page 35 Getting Started, Continued Time Setup 1. From the Main screen, press Setup>Sys>Date/Time to display the ™ Date/Time screen. The iPrep Instrument uses the 24 hour clock. 2. Press 2 to display the Time screen. Setup>Sys>>Time Cur:CH:CM:CS Set:SH:SM:SS Key:Up,Dn,RET,ESC • Row Curr displays the current hour on CH, current minute on CM, and current second on CS respectively, and updates every new minute.
- Page 36 Getting Started, Continued Removing the Important: Do not remove the iPrep ™ Card while the instrument is ON. This stops the ™ automated purification protocol and may cause corruption of liquid handling files. iPrep Card Remove the card as described below.
- Page 37 Instrument to a computer using a RS232C serial cable supplied with the barcode reader. ™ 1. Connect the barcode reader to the BCR inlet on the rear of the iPrep Instrument. For computer For Barcode Reader 2.
- Page 38 1. Insert the CommViewer Barcode software CD-ROM included with the ™ instrument into the CD-ROM drive of the computer (connected to the iPrep Instrument using the RS232C serial cable as described on the previous page). 2. Open the CommViewer folder. Double click the setup.exe file to begin installation of the CommViewer Barcode software.
- Page 39 When reading the barcodes using the barcode reader connected to the ™ ™ iPrep Instrument, the digital display on iPrep screen prompts you to Press 1 to output data. On pressing 1, the barcode data read using the barcode reader appears in the Receive Data window.
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Page 40: Using The Iprep Purification Instrument
™ Using the iPrep Purification Instrument Introduction ™ Instructions are provided in this section to prepare the iPrep Purification ™ ™ Instrument with the iPrep disposables supplied with each iPrep Kit for nucleic acid purification. Materials Needed You will need the following items. Ordering information is on page xvi. - Page 41 Cartridge ™ the iPrep Cartridge Rack. Rack ™ 1. Open the instrument door and remove the iPrep Cartridge Rack and ™ iPrep Tip and Tube Rack from the instrument. ™ 2. Remove the Reaction Cartridges from the iPrep Kit box. You need one Reaction Cartridge per sample.
- Page 42 ™ Using the iPrep Purification Instrument, Continued Assembling the ™ Instructions are provided below to assemble the tips and tubes on the iPrep ™ iPrep Tip and Tip and Tube Rack. Tube Rack ™ ™ 1. Remove the iPrep Sample Tubes from the iPrep Kit box.
- Page 43 You can read the barcode on a set of 3 samples at a time. ™ 1. Connect the barcode reader to the BCR port on the rear of the iPrep Instrument (page 21). This is not needed if you are only recording the sample numbers.
- Page 44 • To only record the sample number without using the barcode ™ reader, type in the number of the sample using the iPrep keypad (0-9). The number keypad allows you to type in the respective number and the Up and Down arrow keys allow you to scroll through the alphabet, in case you wish to read letters.
- Page 45 Successful barcode reading is indicated by an audible tone and a ™ green spot on the code. The barcode appears on line 3 of the iPrep screen 2 as shown on the previous page. 4. To record the elution tube numbers only without using the barcode reader, see Step 7, previous page for details.
- Page 46 Barcode Data ™ barcode data to a computer using the RS232C serial cable connecting the iPrep Instrument to a computer (see page 21 for details). 1. After reading the last set of elution tube barcodes, you have the option to output data.
- Page 47 Ensure that the sample tube without lids (containing the sample) and elution tubes without ™ caps are placed in the correct positions on the iPrep Tip and Tube Rack (see page 26 for details).
- Page 48 To process more samples using another iPrep Protocol Card, close the Purification ™ instrument door and turn the power switch to OFF. Remove the iPrep Protocol, continued ™ Protocol Card as described on page 20. Insert the new iPrep Protocol Card, load the racks with new reagent cartridges, tips, tubes, and sample, and start the protocol as described above.
- Page 49 ™ Using the iPrep Purification Instrument, Continued Manual Screen To manually move the axis or return the tip to the origin after a manual stop: 1. Press 1 to go to the Manual screen from the Main screen. 2. From the Manual screen, you can select 1 to move all axes to the original positions, 2 to return the Tips to Tip Holders and move all axes to original positions, or 3 to clean.
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Page 50: Performing Instrument Tests
Reaction Cartridges Performing Tests ™ 1. Insert the iPrep Protocol Card (available separately, page xvi) into the card slot on the instrument as described on page 16. 2. Turn ON the instrument. The Main screen is displayed. 3. Press 3 to display the Test screen. - Page 51 1. Press 1 from the Test screen. ™ 2. Load the iPrep Cartridge Rack with 13 empty reaction cartridges supplied with the instrument. Place the loaded rack on the iPrep ™ Platform as described on page 25. ™...
- Page 52 Performing Instrument Tests, Continued Temperature Test, 5. Compare the Now Temp value with the desired Set Temp value displayed continued on the screen. The Now Temp value should reach the Set Temp value after a few minutes of starting the test. After reaching the set value, the Alarm value, displayed on the screen should turn to 00.
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Page 53: Cleaning And Maintaining The Iprep Instrument
™ Cleaning and Maintaining the iPrep Instrument Introduction ™ Refer to the instructions below to clean and maintain the iPrep Instrument. Maintenance ™ Two types of maintenance are performed on the iPrep Instrument as listed in Schedule the table. For details on each type of maintenance, see below:... - Page 54 ™ One set of 13 D-Rings are included with the iPrep Instrument. To obtain more D-Rings, contact Technical Support (page 45). 1. Using a small forceps or pliers, remove each D-Ring from the nozzle by pulling out the D-Ring and then sliding out the D-Ring from the nozzle.
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Page 55: Troubleshooting
Support (page 45) for a service engineer. turned on) ™ ™ No display (fan iPrep Card is not Turn OFF the instrument and re-insert the iPrep Card in the ™ turns on when inserted correctly proper orientation into the iPrep Card slot and be sure to the power is on) insert it completely into the slot by manually pushing the card. - Page 56 Rack described on page 25. ™ While loading the tubes on the iPrep Tip and Tube Rack, be sure the tubes are uncapped. If the plasticware are incorrectly loaded, the purification protocol still starts as there are no sensors on the instrument to detect incorrect placements.
- Page 57 Remove any magnetic beads using a magnetic separator ™ containing DNA in the eluate (MagnaRack is available from Invitrogen, see page xvi) or centrifuge the sample in a microcentrifuge for 1 minute at is discolored maximum speed. DNA contaminated Minimize the amount of blood or blood-stained sample used (≤...
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Page 58: Iprep ™ Instrument Error Codes
Instrument Error Codes Introduction ™ This section describes the error codes displayed by the iPrep Instrument when it encounters a problem. 1. Make a note of the error code including the line number and contact Technical Support (page 45). Note that the error codes listed in the table below cannot be fixed by the user. - Page 59 ™ iPrep Instrument Error Codes, Continued Code Problem Code Problem Parameter buffer overflowed Protocol failed to pick up tip Parameter storage buffer pointer Protocol failed to eject tip overflowed Label storage buffer overflowed Protocol failed to aspire Label pointer buffer overflowed...
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Page 60: Appendix
Instructions are provided below to replace the 3.15 A fuse for the main power socket. ™ Note: The 6.3 A fuse included with the iPrep Instrument are for internal parts (CPU, motor driver, heater) of the instrument. Do not attempt to change 6.3 A fuse for these internal parts. -
Page 61: Technical Support
Access to the Invitrogen Online Catalog • Additional product information and special offers Contact Us For more information or technical assistance, call, write, fax, or email. Additional international offices are listed on our Web page (www.invitrogen.com). Corporate Headquarters: Japanese Headquarters: European Headquarters: Invitrogen Corporation... -
Page 62: Purchaser Notification
If the purchaser is not willing to accept the limitations of this limited use statement, Invitrogen is willing to accept return of the product with a full refund. For information on purchasing a license to this product for purposes other than research, contact Licensing Department, Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, California 92008. - Page 63 Purification Instrument (“Instrument”) will be free from defects in materials and Warranty workmanship for a period of one (1) year from the date of delivery. Invitrogen agrees, as its sole responsibility under this limited warranty, and upon prompt notice of a defect, to repair, replace or refund purchase price, at its discretion, any Instrument discovered to be defective within the warranty period.
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