Advertisement

Quick Links

Operation Manual
1400FLi Series
Covering Models:
1482FLi & 1486FLi

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the 1400FLi Series and is the answer not in the manual?

Questions and answers

Summary of Contents for Van Guard 1400FLi Series

  • Page 1 Operation Manual 1400FLi Series Covering Models: 1482FLi & 1486FLi...
  • Page 2: Table Of Contents

    Table of Contents are the flagship of the Vanguard microscope line. Combining top performance with highly-advanced features and optics, the 1400FLi Series models produce fluorescence images of the Introduction: ............2 utmost in clarity. Choose from brightfield or phase contrast/ brightfield/darkfield.
  • Page 3: Parts & Accessories

    Parts & Accessories Included Parts: Model 1482FLi Model 1486FLi Trinocular Head (1 ea.) Trinocular Head (1 ea.) Stand (1 ea.) Stand (1 ea.) Brightfield Condenser (1 ea.) Phase Contrast/Brightfield/Darkfield Condenser (1 ea.) Epi-Illumination Assembly (1 ea.) Phase Contrast Centering Telescope (1 ea.) External Power Supply (1 ea.) Epi-Illumination Assembly (1 ea.) 10X High Eyepoint Eyepieces (2 ea.)
  • Page 4: Optional Accessories

    Parts & Accessories Optional Accessories: Digital Camera Systems: Part Number: Description: 1400-CDPC-10 USB Color Digital Camera System, 10 Megapixels 1400-CDPC-5 USB Color Digital Camera System, 5 Megapixels 1400-CDPC-3 USB Color Digital Camera System, 3 Megapixels Other Accessories: Part Number: Description: Call for Part Numbers Scale Reticle Call for Part Numbers...
  • Page 5: Microscope Diagrams

    1400FLi Series Parts Camera Port Fluorescence Illumination Filter Slider Tube Slider Camera Port Head Assembly Dust Cap Eyepieces with Prism Slider Rubber Eyecups Lamp Focusing Diopter Head Set Knob Adjustment Screw (upper) Mechanism Epi-Illuminator Housing Lamp Lamp Door Focusing Set Knob...
  • Page 6 1400FLi Series Parts 1400FLi Series Base, Left Side View Substage Adjustment Knob Up-Stop Mechanism Variable Lighting Control Power Switch 1400FLi Series Stand, Rear View Carrying Handle Power Cord Jack Pictures and descriptions of the external power supply are shown on page 16.
  • Page 7: Setup

    Setup The next 4 pages are dedicated to assembling a working microscope. The following section, “Using your 1400FLi Series Microscope” (starting on page 13) explains the various features of the microscope and how to use them. Assembly All Models 1. After removing the microscope parts from the protective foam packaging and checking it over for all components and accessories (a list is provided on page 3), you can begin assembly.
  • Page 8 Setup Assembly (continued) 6. Attach the UV shield to the bottom front of the epi-illuminator housing assembly with the UV shield set screw (see figure 4a). Figure 4 7. Slide the eyepieces into the eyetubes and attach eyecups (see figure 5). Figure 5 8.
  • Page 9 Setup Assembly (continued) 11. Slide the neck of the condenser up through the silver ring of the condenser mount until it will go no further (see figure 8). Make sure that the text “NA 1.25” is facing upward. If the condenser will not slide freely through the silver ring do not force, simply wiggle the condenser while lightly pushing up.
  • Page 10: Alignment Of Mercury Lamp

    Setup Assembly (continued) 17. When the large end is in the flexible mounting socket, manipulate the flexible mounting socket to get the small end into the stationary mounting socket (see figure 12). 18. Push the small end in as far as it will go and then tighten the set screw on the stationary mounting socket.
  • Page 11: Aligning The Condenser

    Setup Aligning the Condenser All Models Adjustments to the substage condensing system are crucial for proper illumination and performance. There are three basic adjustments which need to be made: Centering, Vertical Focusing, and Aperture Adjustment. 1. Centering: The condenser must be centered in the light path to ensure proper light control. A simple method for centering is as follows: ...
  • Page 12: Aligning The Phase Contrast Annulus Rings

    Setup Aligning the Phase Contrast Annulus Rings 1486FLi Model Phase contrast is a system which utilizes a series of light baffling annulus rings. Proper alignment of these rings is absolutely necessary to achieve optimal phase contrast. 1. Begin by turning on the substage illuminator with the power switch located on the lower left side of the instrument.
  • Page 13: Using Your 1400Fli Series Microscope

    Using your 1400FLi Series Microscope Focusing and Mechanical Stage Mechanisms All Models 1. Focusing adjustment is achieved by turning the coarse/fine focus controls (see figures 19a and 20a). The large knob is used for coarse adjustment, the smaller knob for fine adjustment. The coaxial arrangement allows for easy, precise adjustment without stage drift.
  • Page 14: Interpupillary & Diopter Adjustments

    Using your 1400FLi Series Microscope Interpupillary and Diopter Adjustments All Models 1. Interpupillary adjustment (the distance between eyepieces) is made through a “folding” action. The Seidentopf design allows for a folding adjustment which is quickly and easily done for each user (see figure 21).
  • Page 15: Using The Camera Port

    (see figure 24). Note: The 1400FLi Series Microscopes utilize a 70/30 split sliding prism. This split prism diverts 70% of the light to the camera port and the remaining 30% to the eyepieces.
  • Page 16: External Power Supply

    Using your 1400FLi Series Microscope External Power Supply All Models 1. Follow the instructions on page 10 to setup the external power supply. WARNING: DO NOT attempt to operate the power supply unless the illuminator unit is connected and has a 100W HBO Mercury lamp in place.
  • Page 17: Fluorescence Viewing

    Using your 1400FLi Series Microscope Fluorescence Viewing All Models WARNING: Avoid prolonged exposure to unfiltered ultraviolet light. 1. Select the desired magnification by choosing the appropriate objective. It is usually easiest to focus initially by using brightfield or phase contrast.
  • Page 18: Maintenance

    Maintenance Replacing the Lower Lamp All Models 1. Before attempting to replace or remove the lamp, UNPLUG THE MICROSCOPE FROM ANY POWER SOURCE AND ALLOW SUFFICIENT TIME FOR THE LAMP TO COOL. 2. Lamp replacement is done by laying the microscope on its back and opening the trap door located on the bottom of the base by pulling on the release knob (see figure 28).
  • Page 19: Specifications

    Specifications 1400FLi Microscopes Viewing Head: Trinocular Viewing Head Type: Seidentopf Head Rotation: 360° Head Inclination: 30° Sliding Prism: 70/30 Split Interpupillary Adjustment: 55-75mm Dioptric Adjustment: -5 to +5 Eyepiece Magnification: 10X Widefield, High Eyepoint Eyepiece Field Diameter: 18.5mm Nosepiece: Quintuple/Reversed [0.10 N.A., 17.0mm W.D., 4.5mm F.O.V.] Fluorescence Objectives (All Models): Achromatic, Infinity...
  • Page 20: Warranty

    Warranty & Service 5 Year Limited Warranty If you experience problems setting up your microscope or have service related questions VanGuard microscopes are warranted by VEE GEE Scientific LLC please contact your dealer or VanGuard to be free from defects in material and workmanship for a period Microscopes directly at 1-800-423-8842.
  • Page 21 www.veegee.com | 800-423-8842 sales@veegee.com | techsupport@veegee.com veegee.com/pages/technical-support-service VGMNL081319-1480FLi Printed in the USA...

This manual is also suitable for:

1482fli1486fli

Table of Contents