Temperature Step - Agilent Technologies SureCycler 8800 Setup And User Manual

4 PCR Programs

Temperature Step

Temperature Step
This program step holds the thermal block at a specified temperature for
a specified time. The user- defined parameters for this step also allow you
to set temperature or time increments (or decrements) with each cycle
and set the rate at which the thermal block reaches the set temperature.
Processing Enter the temperature for the step (in °C).
Temperature
Hold for Enter the duration of the step. Units are hours : minutes : seconds
Temperature Select the ramp rate for the instrument. The Fast ramp rate is the default
Ramp ramp rate for the instrument and is optimal in most PCR protocols. The
Standard ramp rate is provided as an option and could improve
amplification in some cases. Note that the Fast ramp rate improves
performance for some enzymes, including Agilent's Herculase II DNA
Polymerase and PfuUltra II DNA Polymerase.
Step Type Select the type of step from the drop- down. The options are denaturation,
annealing and elongation.
46 Agilent SureCycler 8800 Setup and User's Guide