Optimization Test Guidelines - Agilent Technologies PlateLoc User Manual

Thermal microplate sealer
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Optimization test guidelines

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Test guidelines
Related topics
This topic presents the guidelines to consider when planning seal-
quality optimization tests.
When you plan optimization tests, consider the following:
Always run an optimization test for each type of plate material and
plate design. Plates made from the same material but with different
designs can require different sealing parameters.
Always use spare plates in the optimization tests.
Use a new plate for each seal cycle. The characteristics of a plate
can change through repeated seal cycles, therefore changing the
seal results.
Consider the plate content and fluid level in the plate.
Consider the downstream application. For example, plates used in a
thermal cycler will require different sealing parameters than plates
placed in the freezer.
Consider the downstream uses of the plate. For example, plates that
will be sealed one time and discarded can have a more permanent
seal than a plate that will be reused and resealed repeatedly before
being discarded.
Start an optimization test with a low-temperature and short-duration
setting. Adjust the parameters in small increments.
Check the quality of the seal after each seal cycle to determine
which sealing parameters to adjust and whether you need to use an
insert.
Record the optimal sealing parameters.
For seal selection, insert selection, and other optimization test
guidelines, see the PlateLoc Thermal Plate Sealer Seal Selection Guide.
You can locate the guide on the Velocity11 website at
www.velocity11.com.
For more information about...
Reasons for running optimization
tests
Plate requirements
Seal types
Insert types
Adjusting sealing parameters
Chapter 5: Optimizing seal quality
See...
"Reasons for running optimization
tests" on page 78
"Plate requirements" on page 9
"Seal material" on page 13
"Inserts" on page 10
"Adjusting the sealing parameters" on
page 80
79
PlateLoc User Guide

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