Biotek MicroFlo select Operator's Manual

Biotek MicroFlo select Operator's Manual

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Summary of Contents for Biotek MicroFlo select

  • Page 1 Dispenser MicroFlo Select ™ Operator’s Manual...
  • Page 3 MicroFlo Select™ Microplate Dispenser Operator’s Manual February 2010 © 2010 Part Number 7171000 Revision C ® BioTek Instruments, Inc.
  • Page 4: Notices

    BioTek. Changes made to the information in this document will be incorporated in new editions of the publication. No responsibility is assumed by BioTek for the use or reliability of software or equipment that is not supplied by BioTek or its affiliated dealers.
  • Page 5: Table Of Contents

    Canadian Department of Communications Class A ....xiv User Safety ................xiv Safety Symbols................. xv Introduction................1 The MicroFlo Select Microplate Dispenser ........2 Software ................3 Compatibility with the BioStack Microplate Stacker..... 3 Hardware Features ..............4 Package Contents............... 5 Optional Accessories ..............
  • Page 6 | MicroFlo Select™ Installation ................9 1: Unpack and Inspect the Instrument .........10 Accessories Checklist ............10 2. Remove the shipping hardware..........12 3: Install the MicroFlo’s Components ..........14 Install the Tubing Cassette ............14 Tubing Cassette Diagram............15 Insert the Prime Trough Drain Tube ........18 Prime Trough Insert..............19...
  • Page 7 Test Solutions ..............76 Performing the Precision and Accuracy Tests .........77 Procedure: ................77 Documenting Test Results .............79 MicroFlo Select Dispense Precision & Accuracy Test Worksheet 1 µL Cassette Test...............80 MicroFlo Select Dispense Precision & Accuracy Test Worksheet 5 µL Cassette Test...............81 MicroFlo Select Dispense Precision &...
  • Page 8 | MicroFlo Select™ MicroFlo Select Dispense Precision & Accuracy Test Worksheet 1536 Cassette Test...............86 Troubleshooting..............87 Performance Issues ..............88 Error Codes ................89 Contact BioTek Technical Assistance Center ........90 Service/Technical Assistance Center (TAC) .......90 European Coordination Center/ Authorized European Representative..............90 Error Codes ................
  • Page 9: Contacting Biotek Instruments, Inc

    Contacting BioTek Instruments, Inc. | vii Contacting BioTek Instruments, Inc.  BioTek Instruments, Inc. Highland Park, P.O. Box 998 Winooski, Vermont 05404-0998 USA Customer Service and Sales Internet: www.biotek.com Phone: 888-451-5171 (toll free in the U.S.) 802-655-4740 (outside the U.S.)
  • Page 10: Revision History

    Document Conventions The intent of this operator’s manual is to instruct the new user how to set up and operate BioTek’s MicroFlo Select Microplate Dispenser. To help you read and understand this manual, certain document conventions have been used. This manual uses the following typographic conventions:...
  • Page 11: Intended Use Statement

    The dispense tubing cassettes are autoclavable for those applications requiring a sterile fluid path. The MicroFlo Select™ can operate with standard robotic systems, such as BioTek’s BioStack™ Microplate Stacker. This product may be used for In Vitro Diagnostic, research and development, or other non-clinical purposes.
  • Page 12: Warranty & Product Registration

    MicroFlo Select dispense cassettes are backed by BioTek's Confidence-Plus lifetime warranty. For the lifetime of the cassette, BioTek guarantees the cassette components (Tip Holder, Center Holder, Tube Tensioner, and Tube Organizer) will withstand steam autoclave conditions of 121°C and 1 bar (750 mmHg) without adversely affecting dispense performance.
  • Page 13: Precautions

    Precautions The following precautions are provided to help avoid damage to the instrument: Caution: Service. The instrument should be serviced by BioTek authorized service personnel. Only qualified technical personnel should perform troubleshooting and service procedures on internal components. Caution: Environmental Conditions.
  • Page 14: Ce Mark

    Caution: Do not run the MicroFlo Select without a cassette installed on the pump. Caution: Electromagnetic Environment. Per IEC 61326-2-6 it is the user’s responsibility to ensure that a compatible electromagnetic environment for this instrument is provided and maintained in order that the device will perform as intended.
  • Page 15: Directive 2004/108/Ec: Electromagnetic Compatibility

    Directive 2002/96/EC: Waste Electrical and Electronic Equipment Disposal Notice: This instrument contains printed circuit boards and wiring with lead solder. Dispose of the instrument according to Directive 2002/96/EC, “on waste electrical and electronic equipment (WEEE) or local ordinances.” MicroFlo Select Operator's Manual...
  • Page 16: Directive 2002/95/Ec: Reduction Of Hazardous Substances (Rohs)Xiv

    | MicroFlo Select™ Directive 2002/95/EC: Reduction of Hazardous Substances (RoHS) This instrument is exempt from RoHS requirement per Article 2, Category 9. Directive 98/79/EC: In Vitro Diagnostics  Product registration with competent authorities  Traceability to the U.S. National Institute of Standards and Technology (NIST)
  • Page 17: Safety Symbols

    Off (Supply) Caution (refer to accompanying documents) Arrêt (alimentation) Attention (voir documents Aus (Trennung vom Netz) d’accompanement) Desconectado Achtung siehe Begleitpapiere Aperto (sconnessione dalla Atención (vease los documentos rete di alimentazione) incluidos) Attenzione, consultare la doc annessa MicroFlo Select Operator's Manual...
  • Page 18 | MicroFlo Select™ Warning, risk of electric Warning, risk of crushing or pinching shock Attention, risque d’écrasement et Attention, risque de choc pincement électrique Warnen, Gefahr des Zerquetschens Gefährliche elektrische und Klemmen schlag Precaución, riesgo del machacamiento Precaución, riesgo de y sejeción...
  • Page 19: Introduction

    Chapter 1 Introduction This chapter introduces the MicroFlo Select Microplate Dispenser, and describes its hardware and software features. The MicroFlo Select Microplate Dispenser ........2 Software ................3 Compatibility with the BioStack Microplate Stacker .....3 Hardware Features..............4 Package Contents ..............5 Optional Accessories ..............6 Specifications ................7...
  • Page 20: The Microflo Select Microplate Dispenser

    The intuitive menu-driven software allows you to create and store up to 99 dispense protocols.  The MicroFlo has a low-maintenance design, the result of BioTek’s long history with liquid-handling instruments.  In addition to operating on a stand-alone basis, the MicroFlo is compatible with BioTek’s BioStack™...
  • Page 21: Software

    If you have purchased the BioStack to operate with the MicroFlo, refer to the BioStack Operator’s Manual for instructions on configuring the dispenser to run with the BioStack. If you are interested in purchasing the BioStack, contact your local BioTek dealer for more information. MicroFlo Select Operator's Manual...
  • Page 22: Hardware Features

    Stepper motors provide accurate and repeatable carrier and dispense head positioning.  Plate Carrier and Priming Trough are easily removed for cleaning.  Prime Trough Insert is supplied as an accessory to capture expensive reagents for reuse. BioTek Instruments, Inc.
  • Page 23: Package Contents

     10 cc syringe, and a self-adhesive strip for securing the bag to the back of the instrument.  Part numbers are subject to change over time. Please contact BioTek Customer Care if you have any questions. MicroFlo Select Operator's Manual...
  • Page 24: Optional Accessories

    5 µL steel tip 7170014 7172128 10 µL molded tip 7170010 7172039 7170007 7170020 10 µL steel tip 7170013 7172127  Part numbers are subject to change over time. Please contact BioTek Customer Care if you have any questions. BioTek Instruments, Inc.
  • Page 25: Specifications

    1 x 8, 1 x 12 Standard Microstrips Flat, round, “V” bottom, PCR, or half-area Microwells 12 mm X 75 mm Test tube rack accessory available from BioTek Test tubes is required (PN 7170520) 13 mm X 100 mm Hardware & Environmental Cassette...
  • Page 26: Dispense Precision And Accuracy

    ± 2% @ 10L per well* < 4%CV @ 10L per well ± 4% @ 10L per well 10µL < 2%CV @ 20L per well* ± 2% @ 20L per well*  * These specifications are for these dispense volumes and higher. BioTek Instruments, Inc.
  • Page 27: Installation

    Chapter 2 Installation This chapter includes instructions for setting up the MicroFlo Select Microplate Dispenser and installing its components. Instructions for repackaging the instrument for shipment are also included. 1: Unpack and Inspect the Instrument........10 Accessories Checklist..............10 2. Remove the shipping hardware ..........12 Required Materials ..............12...
  • Page 28: 1: Unpack And Inspect The Instrument

    BioTek. Inspect the shipping box, packaging, instrument, and accessories for signs of damage. If the MicroFlo Select Microplate Dispenser is damaged, notify the carrier and your BioTek representative. Keep the shipping cartons and packing material for the carrier’s inspection.
  • Page 29 Hex wrench for removing shipping hardware Velcro strips for attaching plastic pouch on rear panel ® Optional accessories shipped separately: Liquid Handling Control™ (LHC) software MicroFlo Select Qualification and Maintenance Package Cassette Calibration kit Carrier test tube rack Angled Dispense Kit MicroFlo Select Operator's Manual...
  • Page 30: Remove The Shipping Hardware

    Attach the shipping brackets to the back of the instrument for storage. They will be needed again if the dispenser must be shipped in the future. BioTek Instruments, Inc.
  • Page 31: Remove The Rubber Band

    Use the Velcro strips to attach the plastic storage pouch to the rear panel of the instrument. Select a position that does not block the vent holes. Now, you’re ready to complete the installation by installing its components. MicroFlo Select Operator's Manual...
  • Page 32: 3: Install The Microflo's Components

    Take a moment to identify the MicroFlo’s parts that are named in the instructions for installing the tubing cassette. Pump Pump Cover Dispense arm Stainless steel plate Figure 3: Dispenser ready for cassette installation.  The Pump Cover must be closed to operate the dispenser. BioTek Instruments, Inc.
  • Page 33: Tubing Cassette Diagram

    The tube tensioner’s scale is useful when calibrating the cassette. : At the opposite end of the cassette from the tip holder, the Tube Organizer tube organizer holds the 8 tubes together for inserting into the fluid vessel. MicroFlo Select Operator's Manual...
  • Page 34 Slide the Tip Holder into the dispense arm. Do not twist the tubing. The Tip Holder’s front plate with the BioTek logo faces the Center Holder. Make sure the tip holder is level and snapped into place. BioTek Instruments, Inc.
  • Page 35 When installing the cassettes, guide the steel plate first to the right to align with the tensioner, then, push both pieces clockwise around the pump. Figure 7: Align the Tube Tensioner with the steel plate. MicroFlo Select Operator's Manual...
  • Page 36: Insert The Prime Trough Drain Tube

    Figure 9: Put the Tube Organizer in the fluid supply vessel. Return the Pump Cover to its position covering the pump. Insert the Prime Trough Drain Tube The MicroFlo ships with a tube to drain the priming trough. You must supply a vessel to catch the fluid. BioTek Instruments, Inc.
  • Page 37: Prime Trough Insert

    Figure 11: Prime Trough Insert for capturing precious fluids. The MicroFlo ships with an extra reservoir to capture expensive reagent after priming, rather than discarding it. The Prime Trough Insert (PN 7172062) fits inside the prime trough. MicroFlo Select Operator's Manual...
  • Page 38: Power Supply And Cords

    20 | Installation Power Supply and Cords The MicroFlo Select dispenser uses an external 24-volt power supply that is compatible with 100 - 250 V~; 50-60 Hz. Connect the power cord to the power supply, then connect the power supply’s output plug to the 24-VDC connector on the left side of the instrument.
  • Page 39: 4: Verify Performance

    4: Verify Performance | 21 4: Verify Performance Before using the MicroFlo Select for the first time, verify that it is operating properly by turning it on. The power switch is located on the dispenser’s left side. Each time the dispenser is turned on, it automatically performs a system self-test. This test checks the carrier, cassette status, pump positioning and operation.
  • Page 40: Repacking And Shipping The Microflo

    22 | Installation Repacking and shipping the MicroFlo If you need to ship the MicroFlo Select Microplate Dispenser to BioTek for service or repair, be sure to use the original packing materials. Other forms of commercially available packaging are not recommended and can void the warranty.
  • Page 41 Installation instructions advise attaching the shipping brackets to the back of the dispenser. They should be screwed to the left back corner of the MicroFlo. Contact BioTek Customer Care if you need replacement brackets. Plate carrier shipping bracket Dispense arm shipping bracket Figure 12: Shipping hardware stored on rear panel.
  • Page 42 The bracket will hold the dispense arm just above the plate carrier. Close the Pump Cover and secure it in place with the rubber band. BioTek Instruments, Inc.
  • Page 43: Repacking Illustration

    Securely tape the boxes closed.  Write the RMA in large, clear letters on the outside of the outer box and return to: BioTek Instruments, Inc. ATTN: RMA# xxxxx 100 Tigan Street Highland Park Winooski, VT 05404-0998 USA Illustration 3 MicroFlo Select Operator's Manual...
  • Page 44 26 | Installation BioTek Instruments, Inc.
  • Page 45: Essential Concepts

    Chapter 3 Essential Concepts This chapter expands on the information about the MicroFlo Select Microplate Dispenser provided in the Introduction. It describes hardware and software features. How it works ................28 How to optimize performance ............29 Quick Dispense or Protocol? ............31 Quick Dispense (Main Menu) ...........31...
  • Page 46: How It Works

    28 | Essential Concepts How it works The MicroFlo Select dispenses fluid using a peristaltic pump. It works by expelling a portion of fluid trapped between advancing rollers. The shape of the tubing between the rollers is similar to sausage links in a continuous strand.
  • Page 47: How To Optimize Performance

    For users of 1536-well microplates, especially those designed for imaging, with wells recessed deep within the microplate: you can better position the tips inside the plate, close to the wells' rims by removing the two outer shields from the Tip Holder and lowering the Dispense Height. MicroFlo Select Microplate Dispenser...
  • Page 48 You can use your thumb and forefinger to snap the tab off and away from the tips, but the job is easier when a small set of pliers, a wrench, or similar tool is used to get a good grip on the tab. You can remove the outer shields if necessary. BioTek Instruments, Inc.
  • Page 49: Quick Dispense Or Protocol

    SELECT DISP PROGRAM Second line: Program Name Name: 25U TO 96 Use Options button to scroll In contrast to the Quick Dispense programs, the MicroFlo permanently saves Protocols. Up to 99 Protocols can be created and stored onboard. MicroFlo Select Microplate Dispenser...
  • Page 50 Repeat temporarily changed values, if applicable). BioTek recommends using Protocols for specific applications, regularly run assays, and to comply with GLP. Especially when the MicroFlo is being used by multiple people, protocols will give you more confidence than a Quick Dispense that the desired dispense parameters have been preserved.
  • Page 51: Handling Special Plates And Test Tube Racks

    Conversely, dispense tubes can (and sometimes, must) be removed from the supply vessel (or from the cassette) to prevent them from missing the wells. Testing at BioTek found the following capabilities: Plate Type Columns/Rows Tubes per Well...
  • Page 52: Test Tube Racks

    Test Tube Racks The MicroFlo supports two types of test tubes that fit in a test tube rack (PN 7170520) available from BioTek as an accessory item:  20 - 12 mm X 75 mm, 6 mL capacity tubes. Select when using the keypad.
  • Page 53: Chemical Compatibility

    It is best if the dispenser is rinsed with deionized water after contact with any strong acid, base, or solvent. The following materials are used in the MicroFlo Select dispenser and tubing cassettes. Materials listed as 1-10 in Table 1...
  • Page 54: Table 2. Chemical Compatibility Ratings

    The magnitude of this effect will vary with concentration and exposure. Re-calibration of the cassette may be required.  Please contact BioTek with questions about compatibility of any chemicals not described here. BioTek Instruments, Inc.
  • Page 55: Operation

    Chapter 4 Operation This chapter provides instructions for operating the MicroFlo Select Microplate Dispenser using the keypad. When using the Liquid Handling Control™ (LHC) software refer to its Help system to learn how to control the MicroFlo. Basic Operation ...............38 Turn On/Off the MicroFlo ............38...
  • Page 56: Basic Operation

    LCD display. Press Options to scroll through options or the alphabet. Press Shift+Options to scroll backward through options or the alphabet when Figure 13: Keypad naming a Protocol. BioTek Instruments, Inc.
  • Page 57: Main Menu

    The two line display shows a Quick Dispense in the top line and menu options in the second line. BioTek ships the dispenser from the factory with one Quick Dispense program (described below) installed. It is displayed in the top line: ...
  • Page 58: Dispenser Run Menu

     : press the Softkey under the arrow to move to the next menu: Dispenser Action Menu Dispenser Action Menu From the main menu press the arrow key one time for this menu: Top line DISPENSER ACTION MENU Second line Define Util Setup     Softkeys BioTek Instruments, Inc.
  • Page 59 Util The Utility option offers System Self Check and CheckSum: see Utility Menu Options on page 54. Setup The Setup menu holds configuration and other critical details: see Setup Menu Options on page 53. MicroFlo Select Microplate Dispenser...
  • Page 60: Run A Quick Dispense

    Whenever you define/change dispense parameters and run the routine, the program is saved for reuse. New programs displace the oldest ones. These programs are not named, which distinguishes them from the Protocols. Read a comparison of the Quick Dispense and Protocols in the Essential Concepts chapter. BioTek Instruments, Inc.
  • Page 61: About Dispense Protocols

    Use the table below to learn more about each parameter. Use the Softkeys to select to the last prompt: OK to save program? Return to the Dispenser Run Menu (press Previous Screen ) to run the program. MicroFlo Select Microplate Dispenser...
  • Page 62 Using the LHC: When creating a protocol using the LHC, enter a unique name in the Protocol Name field. And, while they can be different, BioTek recommends using the same name when saving the protocol file. The file will be saved with a .LHC filename extension.
  • Page 63 The MicroFlo assigns the first available number to a newly created protocol. The dispenser ships without any pre-defined protocols, so the first one you create will be numbered 01. The second will be numbered 02, and so on. MicroFlo Select Microplate Dispenser...
  • Page 64 A protocol name must be unique. If the name already exists, the dispenser will require you to change it. This protects programs from inadvertently being overwritten. Clear Entry To delete an entry, press the CLEAR key. BioTek Instruments, Inc.
  • Page 65: Run A Dispense Protocol

    1 µL cassette, is essential for achieving accurate dispense volumes. Press the Prime button on the dispenser in between runs (or add a Prime step to an LHC protocol). Learn ‘How to optimize performance’ in the Essential Concepts chapter. MicroFlo Select Microplate Dispenser...
  • Page 66: Prime The Microflo

    Pre-priming before a dispense run to condition the tips is a standard operating procedure. MicroFlo’s Quick Dispense programs include 2 pre-prime cycles before each run. You cannot change the prime settings of the Quick Dispenses. But, MicroFlo’s Protocols let you define the pre-priming volume and number of cycles. BioTek Instruments, Inc.
  • Page 67: Purge The Microflo

    Press and hold the key until all the fluid in the tubing is evacuated. Using the LHC You can use BioTek’s Liquid Handling Control™ (LHC) software to purge the MicroFlo before or after a dispense run by adding a Purge Step to the protocol. Consult the LHC’s Help to learn more.
  • Page 68: Define The Cassette

    It will hang loosely. Lift the Center Holder out of its slot on the right side of the pump. Hold the Center Holder in one hand while you slide the Tip Holder out of the dispense arm. BioTek Instruments, Inc.
  • Page 69: Define The Plate Type And Plate Map

    (optional accessory). The optimal dispense height and horizontal position are defined onboard for each plate type. The MicroFlo permits adjustments to these values when necessary for special situations. For general use, the predefined parameters allow instant dispensing to all plate types. MicroFlo Select Microplate Dispenser...
  • Page 70 Tools>Instrument Utilities to set the fill pattern Test Tube Racks BioTek provides a test tube rack for use with the MicroFlo as optional accessory. It supports dispensing to two common test tube sizes. Contact your BioTek representative to purchase one.
  • Page 71: Setup Menu Options

    : offers controls for using the BioStack to process plates with the MicroFlo. See Using the BioStack on page 55.  Pattern : is only applicable when processing 384- and 1536-well plates. It lets you select the fill pattern: Column . Select Row for faster throughput. MicroFlo Select Microplate Dispenser...
  • Page 72: Utility Menu Options

    , record the code displayed and press Enter to return to the Checksum options. Repeat for . When running a BioStack, select STKR to obtain the BioStack’s version information. Serial Number Select SN to view the instrument’s serial number. BioTek Instruments, Inc.
  • Page 73: Using The Biostack

    Using the BioStack | 55 Using the BioStack   Dispenser Action Menu> BIOSTACK If you have purchased BioTek’s BioStack™ Microplate Stacker to operate with the MicroFlo, follow the instructions in your BioStack Operator’s Manual to set it up to interact with the dispenser.
  • Page 74 56 | Operation BioTek Instruments, Inc.
  • Page 75: Preventive Maintenance

    Chapter 5 Preventive Maintenance This chapter provides step-by-step instructions for maintaining the MicroFlo Select Microplate Dispenser in top condition, to ensure that it continues to perform to specification. Overview ................58 Recommended Maintenance Schedule.........58 Flush the Tubing Cassette ............59 Record the number of plates processed........60 Clean Exterior Surfaces ............61...
  • Page 76: Overview

    This is necessary to determine when the cassette has reached its expected lifetime and is due for replacement or recalibration. Replacement Tubing Kits, as well as new cassettes are available from BioTek Instruments. Monthly maintenance requires overall cleaning of the dispenser and its accessories, and verifying performance to determine if the cassette needs recalibration.
  • Page 77: Flush The Tubing Cassette

    DI water. Hold the Prime button on the keypad:  1µL cassette = 5 seconds  5µL cassette = 12 seconds  10 µL cassette = 6 seconds. MicroFlo Select Microplate Dispenser...
  • Page 78: Record The Number Of Plates Processed

    You may want to create a form similar to this table to keep track of the volume dispensed with each cassette: Cassette serial #: 2178 Date # Plates Plate Type Volume/Well Total Daily Vol. Total Cassette Vol 10/10/07 5 µL 49920 µL 50 mL 10/11/07 10 µL 31680 µL 82 mL BioTek Instruments, Inc.
  • Page 79: Clean Exterior Surfaces

    Slide the prime trough towards you and away from the dispenser. Soak the trough, trough insert and drain tube in hot sudsy water for at least a half hour. Rinse them thoroughly, and wipe the exterior surfaces with a clean, dry cloth. MicroFlo Select Microplate Dispenser...
  • Page 80: Verify Performance

     Use the Cassette Calibration Kit described in the PDF on the Operator’s Manual CD titled: 7171009_(current Rev)_Calibration Kit Instructions.PDF  Use the Gravimetric Calibration method described in Appendix C of the Operator’s Manual. BioTek Instruments, Inc.
  • Page 81: Unclogging The Dispense Tips

    Thoroughly flush the tubing after/in-between usage, especially when using liquids that crystallize or harden. In case the need arises, BioTek ships a 10 cc plastic syringe with special tubing and fitting for use unclogging tips. Installation instructions recommend storing it in the pouch on the back of the instrument.
  • Page 82 Reinsert the tube into the Tip Holder. Seat the flared edges of the tip into the molded slots.  Replace the Tip Holder cover with its two screws. The etched BioTek label identifies the top of the cover (except for 1536 cassettes’ steel cover plate).
  • Page 83: Replace The Tubing

    (8½” x 14”). Recalibrate the Cassette Cassette Calibration Kit BioTek offers an accessory to the MicroFlo, a Calibration Kit that speeds up the process of recalibrating a cassette. It is also useful to verify performance, one of the recommended monthly maintenance routines.
  • Page 84: Decontamination

    Centers for Disease Control and Prevention (CDC). Neither BioTek nor the CDC assumes any liability for the adequacy of these solutions and methods. Each laboratory must ensure that decontamination procedures are adequate for the Biohazard(s) they handle.
  • Page 85: Tools And Supplies

    BioTek Service Department. Do not soak the keypad. This will cause damage. Wipe the keypad with a damp cloth. Turn off and unplug the MicroFlo Select. Unload the cassette and the prime trough drain tube. Autoclave the cassette. Prepare an aqueous solution of 0.5% sodium hypochlorite (bleach). As an alternative, 70% isopropyl alcohol may be used if the effects of bleach are a concern.
  • Page 86 Soak the drain tube in hot soapy water or the remaining bleach solution for twenty minutes or more. Rinse well and set aside to dry. Discard the used gloves and cloths using a Biohazard trash bag and an approved Biohazard container. BioTek Instruments, Inc.
  • Page 87: Instrument Qualification

    Chapter 6 Instrument Qualification Testing is conducted on every MicroFlo Select Microplate Dispenser to verify dispense accuracy and precision before it leaves BioTek. IQ/OQ/PQ................70 Recommended Qualification Schedule .........71 System Self-Check and Checksum Test........72 System Self-Check..............72 Checksum Test................72 Dispense Precision and Accuracy Tests........73 Materials ................75...
  • Page 88: Iq/Oq/Pq

    OQ tests to monitor for trends.  The successful completion of the OQ procedure, in combination with results that are comparable to previous PQ and OQ tests, confirms that the dispenser is performing consistently over time. BioTek Instruments, Inc.
  • Page 89: Recommended Qualification Schedule

    Recommended Qualification Schedule Task/Test Initially & Initially Monthly Annually Installation and Setup  System Self-Check    Checksum Test    Dispense Precision and Accuracy Test   Run Assay   MicroFlo Select Microplate Dispenser...
  • Page 90: System Self-Check And Checksum Test

    72 | Instrument Qualification System Self-Check and Checksum Test System Self-Check Each time it is turned on, the MicroFlo Select automatically runs the System Self-Check to verify system components. The self-check verifies movement of the dispense arm and plate carrier.
  • Page 91: Dispense Precision And Accuracy Tests

     * These specifications are for these dispense volumes and higher.  Note: The above specifications are for performance. For IQ/PQ/OQ purposes, we have added 1.0% additional tolerance to the %CV to accommodate various test solutions, off-peak wavelengths, reader errors, and pipette errors. MicroFlo Select Microplate Dispenser...
  • Page 92: Performing The Precision And Accuracy Tests

    The tests are fundamentally the same for all cassettes except the 1536: Tare an empty plate on a balance. Use the MicroFlo Select to dispense a quantity of fluid with a known dye concentration to the wells. Weigh the plate to obtain the weight of the fluid dispensed.
  • Page 93: Materials

    READ IN RAPID MODE?”  BioTek blue dye solution, PN 7773001, or equivalent to create the test solutions described on the following page. See also the important notes below:  BioTek determined the pass/fail specifications for the following tests using the test solutions described.
  • Page 94: Test Solutions

     Liquid Test Worksheet templates can be found at the end of this chapter for recording data reduction results. If your tests are failing, this information will be useful for BioTek TAC to help diagnose any problems. Test Solutions Unique concentrations of the test fluid are described here, each one corresponds to a specific dispense volume, except the 1536 Solution for 1536-well testing.
  • Page 95: Performing The Precision And Accuracy Tests

    Make a copy of the applicable worksheet beginning on page 80. Procedure: Install the cassette to be tested. Turn on the MicroFlo Select and make sure the cassette type setting is correct. Turn on the balance. Fill a beaker or other vessel with the test solution.
  • Page 96 Read the plate in an absorbance reader using the dual-wavelength method: read the plate at 630 nm and 450 nm.  If you are using one of BioTek’s keypad-based absorbance readers, such as the ELx800™ or ELx808™, ensure that the reader is not running in Rapid mode.
  • Page 97: Documenting Test Results

    Alternatively, you can purchase the MicroFlo Select Qualification & Maintenance Procedures Kit from BioTek, which contains additional tools for conducting test procedures and recording the results, including logbooks and Microsoft Excel ® ®...
  • Page 98: Microflo Select Dispense Precision & Accuracy Test Worksheet 1 Μl Cassette Test

    80 | Chapter 6: Instrument Qualification MicroFlo Select Dispense Precision & Accuracy Test Worksheet 1 µL Cassette Test 1 µL Dispense Precision Test Standard Deviation (SD):  Mean Absorbance (sum of all wells  % CV (SD Mean x 100) % CV must be <...
  • Page 99: Microflo Select Dispense Precision & Accuracy Test Worksheet 5 Μl Cassette Test

    Performing the Precision and Accuracy Tests | 81 MicroFlo Select Dispense Precision & Accuracy Test Worksheet 5 µL Cassette Test 5 µL Dispense Precision Test Standard Deviation (SD):  Mean Absorbance (sum of all wells  % CV (SD Mean x 100) % CV must be <...
  • Page 100: Microflo Select Dispense Precision & Accuracy Test Worksheet 1 Μl Cassette Test

    82 | Chapter 6: Instrument Qualification MicroFlo Select Dispense Precision & Accuracy Test Worksheet 1 µL Cassette Test 10 µL Dispense Precision Test Standard Deviation (SD):  Mean Absorbance (sum of all wells  % CV (SD Mean x 100) % CV must be <...
  • Page 101: 10 Μl Cassette Test

    Performing the Precision and Accuracy Tests | 83 MicroFlo Select Dispense Precision & Accuracy Test Worksheet 10 µL Cassette Test 10 µL Dispense Precision Test Standard Deviation (SD):  Mean Absorbance (sum of all wells  % CV (SD Mean x 100) % CV must be <...
  • Page 102: Microflo Select Dispense Precision & Accuracy Test Worksheet 5 Μl Cassette Test

    84 | Chapter 6: Instrument Qualification MicroFlo Select Dispense Precision & Accuracy Test Worksheet 5 µL Cassette Test 50 µL Dispense Precision Test Standard Deviation (SD):  Mean Absorbance (sum of all wells  % CV (SD Mean x 100) % CV must be <...
  • Page 103: 10 Μl Cassette Test

    Performing the Precision and Accuracy Tests | 85 MicroFlo Select Dispense Precision & Accuracy Test Worksheet 10 µL Cassette Test 100 µL Dispense Precision Test Standard Deviation (SD):  Mean Absorbance (sum of all wells  % CV (SD Mean x 100) % CV must be <...
  • Page 104 86 | Chapter 6: Instrument Qualification MicroFlo Select Dispense Precision & Accuracy Test Worksheet 1536 Cassette Test 1536 Dispense Precision Test Standard Deviation (SD):  Mean Absorbance (sum of all wells  % CV (SD Mean x 100) % CV must be < 6.0% ...
  • Page 105: Troubleshooting

    Chapter 7 Troubleshooting This chapter offers suggestions for troubleshooting performance problems with the MicroFlo Select Microplate Dispenser. Performance Issues..............88 Error Codes ................89 Contact BioTek Technical Assistance Center.........90...
  • Page 106: Performance Issues

    Viscous fluids sticking to tips Clean the Tips (See the Preventive Maintenance chapter.) Check the cabling (See next page.) Cannot communicate with Select the correct COM Port computer Turn on dispenser; display Main Menu BioTek Instruments, Inc.
  • Page 107: Error Codes

    Appendix A provides a complete list of the error codes with a brief technical description. If this section does not cover the error code displayed by your dispenser, contact BioTek TAC (next page) for assistance. Corrective Action Homing the MicroFlo can reset its internal sensors and clear many errors.
  • Page 108: Contact Biotek Technical Assistance Center

    BioStack. The BioStack Alignment Kit includes the required serial cable for this purpose. Contact BioTek Technical Assistance Center If you cannot resolve technical or performance issues with the MicroFlo Select from the information provided, contact BioTek: Service/Technical Assistance Center (TAC) Phone: 800-242-4685 (toll free in the U.S.)
  • Page 109: Error Codes

    Appendix A Error Codes This section lists the MicroFlo’s error codes with a brief description of how to resolve them, if possible. Contact BioTek TAC for additional assistance. Instrument Error Codes ............92 Software Error Codes ...............94...
  • Page 110: Error Codes

    Upon restarting the dispenser, you should be able to enter commands using the keypad or the LHC. If not, contact BioTek TAC for support.  Use the table below to decipher the error code. For example, error code 304 means the “Pump Cover is not in the safe position (closed...
  • Page 111 DEV_NOT_AVAIL_ERR A100 <device> not available CODE_VERSION_ERR A200 Version strings for multiple uProcessors do not match POWER_SUPPLY_ERR A300 <test type> power supply level error MALLOC_ERR A400 Malloc failed DISPLAY_IN_USE A500 Multiple tasks attempted to use display simultaneously MicroFlo Select Microplate Dispenser...
  • Page 112: Software Error Codes

    Unauthorized to open the COM port 6057 Out of range parameter for the open port function 6058 Unable to open the COM port 6059 Unable to clear the transmission buffer 6060 Unable to close the port 6061 Port is no longer available BioTek Instruments, Inc.
  • Page 113: Dispensing To Test Tubes

    Dispensing to Test Tubes This section provides detailed instructions for operating the MicroFlo Select with the optional test tube rack for dispensing to 12 mm X 75 mm, 6 mL capacity tubes or 13 mm X 100 mm, 9 mL capacity tubes.
  • Page 114: Install The Test Tube Rack

    Reinstall the priming trough and drain tube.  Note: Only four tubes are used to dispense to the test tube rack. Tubes 1, 3, 5, 7 must be removed from the fluid vessel before beginning a dispense. BioTek Instruments, Inc.
  • Page 115: Dispensing To The Test Tube Rack

    Dispensing to the test tube rack | 97 Dispensing to the test tube rack  Important: Read the Special Plates section in the Essential Concepts chapter before dispensing to test tubes. The MicroFlo supports two types of test tubes in the test tube rack: ...
  • Page 116 98 | Dispensing to Test Tubes BioTek Instruments, Inc.
  • Page 117: Gravimetric Calibration Instructions

    Appendix C Gravimetric Calibration Instructions This appendix provides an overview and step-by-step instructions for gravimetrically calibrating a cassette. This procedure can also be used to meet the monthly requirement to “Verify Performance.” Calibrating the Tubing Cassette Gravimetrically......100 About Calibrating Cassettes Gravimetrically .........105...
  • Page 118: Calibrating The Tubing Cassette Gravimetrically

    To ensure precise and accurate dispensing, the tubing cassette must be calibrated. This procedure is one of several options for meeting this requirement. You can purchase calibrated cassettes from BioTek, rather than recalibrate a used cassette. Or, you can purchase a MicroFlo accessory from BioTek: the Calibration Kit, PN 7170017.
  • Page 119: Procedure

    Calibrating the Tubing Cassette Gravimetrically | 101 Figure 1: A MicroFlo Select in preparation for the gravimetric cassette calibration, and a digital precision balance with 0.0001 g resolution. Procedure: Install the cassette to be calibrated. Turn on the MicroFlo Select and make sure the cassette type setting is correct.
  • Page 120 Put the tube organizer into the test fluid supply vessel. Prime the tubing until any visible air bubbles are removed. the dispense protocol. Figure 3: Measure and record the weight of each of the strips after dispensing. MicroFlo Select Microplate Dispenser...
  • Page 121 Figure 4: Use the screwdriver shipped with the MicroFlo to adjust the tension in the tubing. Repeat the process, beginning at Step 5, until the desired Accuracy % is achieved. BioTek Instruments, Inc.
  • Page 122: Gravimetric Cassette Calibration Worksheet

    Gravimetric Cassette Calibration Worksheet Cassette Type: _________ (1µL, 5µL, 10µL) Correction Factor (k): ____________ Density (): ____________ (g/µL) Number of Wells: __________ Volume per well (V): ___________ (µL) Number of Runs: __________         ...
  • Page 123: About Calibrating Cassettes Gravimetrically

    The gravimetric calibration method uses known values (the expected mass of a volume of water/fluid) to set the accuracy of each dispense tube. BioTek ships a 12 X 8 strip plate with the MicroFlo for this purpose. In this procedure, each strip is tared before dispensing and weighted again after dispensing.
  • Page 124 Guidelines for Adjusting the Tension Screw It is a trial and error process determining the correct position of a tube’s tension screw. BioTek developed these guidelines to help, but hands-on experience is the only way to become proficient at it.
  • Page 125  When working with a newly built cassette, use the numbered scale on the Tube Tensioner’s face to evenly line up the blocks. Calibration will be easier when all the tubes begin at the same point. BioTek Instruments, Inc.
  • Page 126 108 | Gravimetric Calibration Instructions MicroFlo Select Microplate Dispenser...
  • Page 127: Index

    CLEAR key ........46 Bio-Stack Columns compatibility .......1 Dispensing to......51 Bio-Stack Communication compatibility .......3 with computer ......20 BioTek Contacting BioTek ......vii Contacting ........ vii Customer Service ......vii European Representative ..vii Service........vii Bleach solution .........67...
  • Page 128 ......7 European Coordination Centervii, 90 LCD............ 38 Letters, selecting via keypad ..46 Liabilities..........ii Fill Pattern.........52 Liquid Handling Control software.. 6 Flow Rate ..........44 Fluid How to reclaim ......49 Main Menu ......27, 31, 38 MicroFlo Select Microplate Dispenser...
  • Page 129 Power Supply .......9, 20 prime.......... 19 precious fluids ........19 Revision history ......viii Pre-Prime...........48 RMA number........22 preserve expensive reagent ....49 RS-232 Preventative maintenance Port..........20 Tools and supplies ....59 Ryton ..........35 Previous Screen key......40 Prime BioTek Instruments, Inc.
  • Page 130 Utility Menu ........54 TAC ..........vii, 90 Technical assistance Verify Performance....21, 62 Europe........90 Technical Assistance....... vii Technical Assistance XE "Technical Warnings..........x assistance:Europe" XE Worksheets "Support:Technical" XE Liquid Test ........ 80 "Service:Technical" Center..90 Technical support......vii MicroFlo Select Microplate Dispenser...

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